{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:35:29Z","timestamp":1790019329345,"version":"4.0.1"},"reference-count":52,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bulgarian Ministry of Education and Science","award":["70-25-40\/23.01.2023"],"award-info":[{"award-number":["70-25-40\/23.01.2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The extreme Antarctic environment offers unique living conditions. The organisms inhabiting Antarctica have a specific metabolism through which they overcome the challenges of large temperature amplitudes, long periods of light or darkness, and intense UV radiation. Microorganisms are ubiquitous in Antarctica and have been the subject of intensive research in recent decades. In this manuscript, two yeast species were isolated from Antarctic habitats and identified as Dioszegia sp. AL105 (AL105) and Bannozyma sp. AL104 (AL104). The cultivation of these two species under submerged conditions were evaluated. The strain AL104 managed to accumulate over 7 g\/L biomass for 96 h. For the first time, the cytotoxic activity of the obtained yeast extracts on malignant cell lines has been studied. The obtained methanol and acetone extracts showed different cytotoxicity when used to treat different malignant cell lines, with maximal values of IC50 = 113 \u00b1 8.2 established for the HUT-78 line with AL104. The methanol extracts of both yeast species exhibited low antibacterial activity against Staphyloccocus aureus, MRSA, and Escherichia coli. Additionally, the methanol extract of AL105 inhibited the biofilm formation of MRSA to a higher extent than the methanol extract from AL104. Antioxidant activities were determined by using HPSA, HRSA, and NOSA tests. The chemical characterization of the extracts showed the presence of various lipids\u2014CoQ10, triglycerides, phosphatidylethanolamine, and ceramide. It is likely that the interaction between the different bioactive molecules can explain the observed cytotoxicity. The current new studies have proven that Antarctic yeasts are a potential source of biologically active molecules with the potential for future biomedical research.<\/jats:p>","DOI":"10.3390\/molecules31142486","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:55:59Z","timestamp":1784249759000},"page":"2486","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Unveiling the Chemical Composition and Biological Activity of Extracts from the Antarctic Yeast Dioszegia sp. AL105 and Bannozyma sp. AL104"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9976-2552","authenticated-orcid":false,"given":"Snezhana","family":"Rusinova-Videva","sequence":"first","affiliation":[{"name":"Department of Biotechnology, The Stephan Angeloff Institute of Microbiology\u2014Bulgarian Academy of Sciences (BAS), 139 Ruski Blvd., 4000 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya Margaritova","family":"Zaharieva","sequence":"additional","affiliation":[{"name":"Department of Infectious Microbiology, The Stephan Angeloff Institute of Microbiology\u2014Bulgarian Academy of Sciences (BAS), 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1952-9903","authenticated-orcid":false,"given":"Dimitrina","family":"Zheleva-Dimitrova","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7748-4133","authenticated-orcid":false,"given":"Vesela","family":"Lozanova","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry, Faculty of Medicine, Medical University of Sofia, 2 Zdrave Str., 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1254-2419","authenticated-orcid":false,"given":"Reneta","family":"Gevrenova","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Lozanov","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry, Faculty of Medicine, Medical University of Sofia, 2 Zdrave Str., 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0675-1652","authenticated-orcid":false,"given":"Stefka","family":"Nachkova","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry and Computer Chemistry, University of Plovdiv \u201cPaisii Hilendarski\u201d, 4000 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6569-8348","authenticated-orcid":false,"given":"Dimitar","family":"Bojilov","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, University of Plovdiv \u201cPaisii Hilendarski\u201d, 4000 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsvetislava","family":"Kamenova","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, The Stephan Angeloff Institute of Microbiology\u2014Bulgarian Academy of Sciences (BAS), 139 Ruski Blvd., 4000 Plovdiv, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3221-2545","authenticated-orcid":false,"given":"Hristo","family":"Najdenski","sequence":"additional","affiliation":[{"name":"Department of Infectious Microbiology, The Stephan Angeloff Institute of Microbiology\u2014Bulgarian Academy of Sciences (BAS), 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spiro","family":"Konstantinov","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Buzzini, P., and Margesin, R. (2014). Cold-adapted yeasts in Antarctic deserts. Cold-Adapted Yeasts. Biodiversity, Adaptation Strategies and Biotechnological Significance, Springer.","DOI":"10.1007\/978-3-642-39681-6"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Carrasco, M., Rozas, J.M., Barahona, S., Alcaino, J., Cifuentes, V., and Baeza, M. (2012). Diversity and extracellular enzymatic activities of yeasts isolated from King George Island, the sub-Antarctic region. BMC Microbiol., 12.","DOI":"10.1186\/1471-2180-12-251"},{"key":"ref_3","first-page":"50","article-title":"Purification and characterization of a novel \u03b1-glucosidase from an Antarctic yeast Dioszegia fristingensis isolate","volume":"1","author":"Carrasco","year":"2017","journal-title":"Amilase"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Yuivar, Y., Barahona, S., Alcaino, J., Cifuentes, V., and Baeza, M. (2017). Biochemical and thermodynamical characterization of glucose oxidase, invertase, and alkaline phosphatase secreted by Antarctic yeasts. Front. Mol. Biosci., 4.","DOI":"10.3389\/fmolb.2017.00086"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1007\/s00792-013-0577-x","article-title":"On the potential of mass spectrometry-based metabolite profiling approaches to the study of biochemical adaptation in psychrophilic yeast","volume":"17","author":"Dalluge","year":"2013","journal-title":"Extremophiles"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1016\/S0196-9781(01)00488-0","article-title":"The intracellular function of extracellular signaling peptides","volume":"22","author":"Lazazzera","year":"2001","journal-title":"Peptides"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1590\/S1517-83822011000300012","article-title":"The diversity, extracellular enzymatic activities and photoprotective compounds of yeasts isolated in Antarctica","volume":"42","author":"Vaz","year":"2011","journal-title":"Braz. J. Microb."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1039\/b310608j","article-title":"Constitutive and UV-inducible synthesis of photoprotective compounds (carotenoids and mycosporines) by freshwater yeasts","volume":"3","author":"Libkind","year":"2004","journal-title":"Photochem. Photobiol. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Buzzini, P., and Margesin, R. (2014). Production of pigments and photo-protective compounds by cold-adapted yeasts. Cold-Adapted Yeasts. Biodiversity, Adaptation Strategies and Biotechnological Significance, Springer.","DOI":"10.1007\/978-3-642-39681-6"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Da Silva, M., Da Silva, A., Fernandez, P., Montone, R., Alves, R., De Queiroz, A., De Oliveira, V., Santos, V., Putzke, J., and Rosa, L. (2022). Extracellular hydrolytic enzymes produced by yeasts from Antarctic lichens. An. Acad. Bras. Cienc., 94.","DOI":"10.1590\/0001-3765202220210540"},{"key":"ref_11","first-page":"24","article-title":"Application of Candida antarctica lipases in the preparation of diacylglycerol","volume":"45","author":"Hou","year":"2026","journal-title":"J. Food Sci. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kan, G., Zhang, Y., Zhang, H., Yu, K., Jiang, Y., Jiang, J., Qiao, L., and Shi, C. (2025). Proteomic analysis of Antarctic sea-ice yeast Rhodotorula mucilaginosa AN5 responding to hyper- and hyposalinity stress. World J. Microbiol. Biotechnol., 41.","DOI":"10.1007\/s11274-025-04472-x"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s10534-021-00350-9","article-title":"Metabolomics reveals the mechanism of Antarctic yeast Rhodotorula mucliaginosa AN5 to cope with cadmium stress","volume":"35","author":"Zhang","year":"2022","journal-title":"Biometals"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Rusinova-Videva, S., Ognyanov, M., Alipieva, K., Nachkova, S., Gerginova, D., Petrova, A., Marudova, M., Milenkova, S., Paunova-Krasteva, T., and Mateev, D. (2024). Biosynthetic capabilities of Antarctic yeast Sporobolomyces roseus AL103: Temperature influence on intracellular metabolites and characterization of the exopolysaccharide. Res. Microbiol., 17.","DOI":"10.1016\/j.resmic.2024.104247"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6103","DOI":"10.1007\/s00253-016-7611-8","article-title":"Rhodotorula glutinis-potential source of lipids, carotenoids, and enzymes for use in industries","volume":"100","author":"Kot","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.foodchem.2019.05.068","article-title":"Identification and quantification of new isomers of isopropyl-malic acid in wine by LC-IT and LC-Q-Orbitrap","volume":"294","author":"Ricciutelli","year":"2019","journal-title":"Food Chem."},{"key":"ref_17","unstructured":"Shen, L., Zhang, X., Yao, J., Zhong, Q., Huang, T., Kawano, S., and Hashi, Y. (2016). Targeted metabonomic study of plasma from rats with acute colitis using LCMS-IT-TOF based metabonomics. Pittcon, 1720\u20131722."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.aca.2018.07.002","article-title":"A rapid LC-MS method for qualitative and quantitative profiling of plant apocarotenoids","volume":"1035","author":"Mi","year":"2018","journal-title":"Anal. Chim. Acta"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"\u00c7i\u00e7ek, S., Mangoni, A., Hanschen, F.S., Agerbirk, N., and Zidorn, C. (2024). Essentials in the acquisition, nterpretation, and reporting of plant metabolite profiles. Phytochemistry, 220.","DOI":"10.1016\/j.phytochem.2024.114004"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1099\/ijs.0.015412-0","article-title":"Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica","volume":"60","author":"Conell","year":"2010","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1111\/1574-6968.12217","article-title":"Cold adaptation of fungi obtained from soil and lake sediment in the Skarvsnes ice-free area, Antarctica","volume":"346","author":"Tsudji","year":"2013","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"De Mendez, G., Lopes, F., Santos, K., Silva, M., Convey, P., Camara, P., and Rosa, L. (2024). Fungal diversity present in snow sampled in summer in the north-west Antarctic Peninsula and the South Shetland Islands, Maritime Antarctica, assessed using metabarcoding. Extremophiles, 28.","DOI":"10.1007\/s00792-024-01338-2"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.simyco.2015.12.002","article-title":"Phylogenetic classification of yeasts and related taxa within Pucciniomycotina","volume":"81","author":"Wang","year":"2015","journal-title":"Stud. Mycol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1002\/jctb.6321","article-title":"Antarctic yeast Cryptococcus laurentii (AL65): Biomass and exopolysaccharide production and biosorption of metals","volume":"95","author":"Nachkova","year":"2020","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Rusinova-Videva, S., Ognyanov, M., Georgiev, Y., Kambourova, M., Adamov, A., and Krasteva, V. (2022). Production and chemical characterization of exopolysaccharides by Antarctic yeasts Vishniacozyma victoriae and Tremellomycetes sp.. Appl. Sci., 12.","DOI":"10.3390\/app12041805"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1159\/000509281","article-title":"Pathogenesis and Therapy of Primary Cutaneous T-Cell Lymphoma: Collegium Internationale Allergologicum (CIA) Update 2020","volume":"181","author":"Bobrowicz","year":"2020","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/j.det.2015.05.011","article-title":"Extracorporeal Photopheresis in the Treatment of Mycosis Fungoides and S\u00e9zary Syndrome","volume":"33","author":"Zic","year":"2015","journal-title":"Dermatol. Clin."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Chiujdea, S., Ferro, M., Vartolomei, M.D., Lucarelli, G., Bekku, K., Matsukawa, A., Parizi, M.K., Klemm, J., Tsuboi, I., and Fazekas, T. (2024). Epirubicin and Non-Muscle Invasive Bladder Cancer Treatment: A Systematic Review. J. Clin. Med., 13.","DOI":"10.20944\/preprints202405.1432.v1"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1038\/nchembio.85","article-title":"Reconciling the chemistry and biology of reactive oxygen species","volume":"4","author":"Winterbourn","year":"2008","journal-title":"Nat. Chem. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6625","DOI":"10.1021\/jp912001c","article-title":"Mechanism of the OH radical scavenging activity of nordihydroguaiaretic acid: A combined theoretical and experimental study","volume":"114","author":"Galano","year":"2010","journal-title":"J. Phys. Chem. B"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1089\/jmf.2004.7.343","article-title":"The evaluation of nitric oxide scavenging activity of certain Indian medicinal plants in vitro: A preliminary study","volume":"7","author":"Jagetia","year":"2004","journal-title":"J. Med. Food"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1023\/A:1005963400984","article-title":"Significance of nitric oxide in carcinogenesis, tumor progression and cancer therapy","volume":"17","author":"Lala","year":"1998","journal-title":"Cancer Metastasis Rev."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Torrez-Benitez, A., Ortega-Valencia, J., Jara-Pinuer, N., Sanchez, M., Vargas-Arana, G., Gomez-Serranillos, M., and Simirgi-otis, M. (2023). Antioxidant and antidiabetic activity and phytoconstituents of lichen extracts with temperate and polar distri-bution. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1251856"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s00253-003-1309-4","article-title":"Cleavage of \u03b2, \u03b2-carotene to flavor compounds by fungi","volume":"62","author":"Zorn","year":"2003","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_35","first-page":"1127","article-title":"Antiproliferative and cytotoxic effects on malignant melanoma cells of essential oils from the aerial parts of Genista sessilifolia and G. tinctoria","volume":"5","author":"Rigano","year":"2010","journal-title":"Nat. Prod. Comm."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1007\/s00284-015-0928-1","article-title":"Tolerance to ultraviolet radiation of psychrotolerant yeasts and analysis of their carotenoid, mycosporine, and ergosterol content","volume":"72","author":"Villareal","year":"2016","journal-title":"Curr. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s12010-012-9983-2","article-title":"Production of metabolites with antioxidant and emulsifying properties by Antarctic Strain Sporobolomyces salmonicolor AL1","volume":"169","author":"Dimitrova","year":"2013","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Kot, A., Blazejak, S., Gientka, I., Kieliszek, M., and Brys, J. (2018). Torulene and torularhodin: \u201cnew\u201d fungal carotenoids for industry?. Microb. Cell Fact., 17.","DOI":"10.1186\/s12934-018-0893-z"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s002530000570","article-title":"Production of \u03b2-carotene by a mutant of Rhodotorula glutinis","volume":"55","author":"Bhosale","year":"2001","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Xie, Z.-T., Mi, B.-Q., Luq, Y.-J., Chen, M.-T., and Ye, Z.-W. (2024). Research progress on carotenoid production by Rhodosporidium toruloides. Appl. Microb. Biotechnol., 108.","DOI":"10.1007\/s00253-023-12943-0"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/0005-2760(92)90005-G","article-title":"Immunogenic properties of man-nose-containing ceramide disaccharide and immunochemical detection of its hapten in the two kinds of crustacean, Euphausia superba and Macrobrachium nipponense","volume":"1123","author":"Itonori","year":"1992","journal-title":"Biochim. Biophys. Acta (BBA)\u2014Lipids Lipid Metab."},{"key":"ref_42","first-page":"169","article-title":"Biological activity of ceramides and other sphingolipids","volume":"29","author":"Stepulak","year":"2012","journal-title":"Adv. Dermatol. Allergol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.4155\/fmc.13.107","article-title":"A review of ceramide analogs as potential anticancer agents","volume":"5","author":"Liu","year":"2013","journal-title":"Future Med. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1038\/s41418-022-00931-2","article-title":"Externalized phosphatidylinositides on apoptotic cells are eat-me signals recognized by CD14","volume":"29","author":"Kim","year":"2022","journal-title":"Cell Death Differ."},{"key":"ref_45","unstructured":"(2017). Biological Evaluation of Medical Devices\u2014Part 5: Tests for In Vitro Cytotoxicity (Standard No. ISO 10993-5:2009)."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_47","unstructured":"(2006). Clinical Laboratory Testing and In Vitro Diagnostic Test Systems\u2014Susceptibility Testing of Infectious Agents and Evaluation of Performance of Antimicro-bial Susceptibility Test Devices\u2014Part 1: Reference Method for Testing the In Vitro Activity of Antimicrobial Agents against Rapidly Growing Aerobic Bacteria Involved in Infectious Diseases (Standard No. ISO 20776\/1-2006). Technical Committee CEN\/TC 140, Technical Committee ISO\/TC 21."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1111\/j.1600-0463.2007.apm_630.x","article-title":"Quantification of biofilm in microtiter plates: Over-view of testing conditions and practical recommendations for assessment of biofilm production by staphylococci","volume":"115","author":"Stepanovic","year":"2007","journal-title":"APMIS"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Manolov, S., Ivanov, I., and Bojilov, D. (2022). Synthesis of New 1,2,3,4-Tetrahydroquinoline Hybrid of Ibuprofen and Its Biological Evaluation. Molbank, 2022.","DOI":"10.3390\/M1350"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1016\/j.foodchem.2011.02.030","article-title":"Antioxidant activities of extract and fractions from Tuber indicum Cooke & Massee","volume":"127","author":"Guo","year":"2011","journal-title":"Food Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1006\/bbrc.1994.1764","article-title":"The Nitric Oxide-Scavenging Properties of Ginkgo biloba Extract EGb 761","volume":"201","author":"Marcocci","year":"1994","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"W566","DOI":"10.1093\/nar\/gkv468","article-title":"Clustvis: A web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap","volume":"4","author":"Metsalu","year":"2015","journal-title":"Nucleic Acids Res."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/14\/2486\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:56:17Z","timestamp":1784249777000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/14\/2486"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,16]]},"references-count":52,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["molecules31142486"],"URL":"https:\/\/doi.org\/10.3390\/molecules31142486","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,16]]}}}