{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:46:26Z","timestamp":1791009986977,"version":"4.1.0"},"reference-count":206,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["AP23488557"],"award-info":[{"award-number":["AP23488557"]}]},{"award":["AP23488557"],"award-info":[{"award-number":["AP23488557"]}],"id":[{"id":"https:\/\/ror.org\/05tyne317","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Recent advances in vaccinology have accelerated the shift from conventional live-attenuated and inactivated vaccines toward subunit and nucleic acid-based platforms. Although these next-generation vaccines offer improved safety, rapid adaptability, and manufacturing flexibility, their relatively low intrinsic immunogenicity often requires efficient adjuvants and delivery systems. Nanoparticle-based vaccine platforms have therefore emerged as versatile tools capable of protecting antigens, improving targeted delivery, and modulating both innate and adaptive immune responses. This review summarizes the major classes of nanovaccine platforms, including lipid and polymeric nanoparticles, self-assembling protein nanostructures such as virus-like particles and ferritin nanocages, saponin-based self-assembling complexes, and inorganic nanomaterials. Particular attention is given to how vaccine performance is determined not only by material composition but also by nanoparticle physicochemical properties, biodistribution, cellular uptake, and mechanisms of immune activation. We further discuss the major challenges limiting clinical translation, including scalable manufacturing, safety evaluation, quality control, regulatory requirements, and long-term biocompatibility. Finally, emerging strategies involving hybrid and personalized nanovaccine platforms are highlighted, illustrating how nanotechnology and immunoengineering are transforming vaccine development for both prophylactic and therapeutic applications.<\/jats:p>","DOI":"10.3390\/molecules31142501","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T02:32:08Z","timestamp":1784514728000},"page":"2501","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Nanoparticle Engineering in Modern Vaccinology: From Delivery Platforms to Immune-Programming Architectures"],"prefix":"10.3390","volume":"31","author":[{"given":"Andrey","family":"Bogoyavlenskiy","sequence":"first","affiliation":[{"name":"Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2648-8975","authenticated-orcid":false,"given":"Vladimir","family":"Berezin","sequence":"additional","affiliation":[{"name":"Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madina","family":"Alexyuk","sequence":"additional","affiliation":[{"name":"Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3638-3341","authenticated-orcid":false,"given":"Pavel","family":"Alexyuk","sequence":"additional","affiliation":[{"name":"Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elmira","family":"Omirtayeva","sequence":"additional","affiliation":[{"name":"Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Brumbaugh, K.Q., Gellert, F., and Mokdad, A.H. (2025). Understanding Vaccine Hesitancy: Insights and Improvement Strategies Drawn from a Multi-Study Review. Vaccines, 13.","DOI":"10.3390\/vaccines13101003"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Rodrigues, C.M.C., and Plotkin, S.A. (2020). Impact of Vaccines; Health, Economic and Social Perspectives. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01526"},{"key":"ref_3","first-page":"S1","article-title":"The Importance of Immunization as a Public Health Instrument","volume":"99","year":"2022","journal-title":"J. Pediatr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/s41577-020-00479-7","article-title":"A Guide to Vaccinology: From Basic Principles to New Developments","volume":"21","author":"Pollard","year":"2021","journal-title":"Nat. Rev. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s43556-025-00301-7","article-title":"Mucosal Immunity and Vaccination Strategies: Current Insights and Future Perspectives","volume":"6","author":"Zhang","year":"2025","journal-title":"Mol. Biomed."},{"key":"ref_6","first-page":"55","article-title":"Nanotechnology-Based Strategies for Vaccine Development: Accelerating Innovation and Delivery","volume":"6","author":"Cheng","year":"2025","journal-title":"Biomater. Transl."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Saleh, M., El-Moghazy, A., Elgohary, A.H., Saber, W.I.A., and Helmy, Y.A. (2025). Revolutionizing Nanovaccines: A New Era of Immunization. Vaccines, 13.","DOI":"10.3390\/vaccines13020126"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Ghattas, M., Dwivedi, G., Lavertu, M., and Alameh, M.-G. (2021). Vaccine Technologies and Platforms for Infectious Diseases: Current Progress, Challenges, and Opportunities. Vaccines, 9.","DOI":"10.3390\/vaccines9121490"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"24650","DOI":"10.1021\/acsnano.3c10797","article-title":"Nanoplatform Based Intranasal Vaccines: Current Progress and Clinical Challenges","volume":"18","author":"Bai","year":"2024","journal-title":"ACS Nano"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41565-020-0739-9","article-title":"Advances in Nanomaterial Vaccine Strategies to Address Infectious Diseases Impacting Global Health","volume":"16","author":"Fries","year":"2021","journal-title":"Nat. Nanotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1080\/14760584.2021.1984890","article-title":"Nanovaccine: An Emerging Strategy","volume":"20","author":"Das","year":"2021","journal-title":"Expert. Rev. Vaccines"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"He, J., Xiao, W., Hua, D., Liu, M., Guo, H., Xu, L., Xiao, M., Du, Y., and Li, J. (2025). Nanovaccines: Innovative Advances from Design Strategies to Clinical Translation. Vaccines, 13.","DOI":"10.3390\/vaccines13090900"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1038\/nbt1332","article-title":"Exploiting Lymphatic Transport and Complement Activation in Nanoparticle Vaccines","volume":"25","author":"Reddy","year":"2007","journal-title":"Nat. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1038\/nri2868","article-title":"Vaccine Delivery: A Matter of Size, Geometry, Kinetics and Molecular Patterns","volume":"10","author":"Bachmann","year":"2010","journal-title":"Nat. Rev. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3657","DOI":"10.1016\/j.biomaterials.2010.01.065","article-title":"Effects of Particle Size and Surface Charge on Cellular Uptake and Biodistribution of Polymeric Nanoparticles","volume":"31","author":"He","year":"2010","journal-title":"Biomaterials"},{"key":"ref_16","first-page":"5577","article-title":"The Role of Surface Charge in Cellular Uptake and Cytotoxicity of Medical Nanoparticles","volume":"7","year":"2012","journal-title":"Int. J. Nanomed."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7155","DOI":"10.1021\/nn201950e","article-title":"Nanoparticle Size Is a Critical Physicochemical Determinant of the Human Blood Plasma Corona: A Comprehensive Quantitative Proteomic Analysis","volume":"5","author":"Tenzer","year":"2011","journal-title":"ACS Nano"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1007\/s11095-008-9626-z","article-title":"Shape Induced Inhibition of Phagocytosis of Polymer Particles","volume":"26","author":"Champion","year":"2009","journal-title":"Pharm. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.nantod.2014.04.008","article-title":"Challenges Associated with Penetration of Nanoparticles across Cell and Tissue Barriers: A Review of Current Status and Future Prospects","volume":"9","author":"Barua","year":"2014","journal-title":"Nano Today"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1208\/s12248-015-9780-2","article-title":"A Review of Clinical Translation of Inorganic Nanoparticles","volume":"17","author":"Anselmo","year":"2015","journal-title":"AAPS J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3169","DOI":"10.1021\/acsnano.5b00147","article-title":"Elasticity of Nanoparticles Influences Their Blood Circulation, Phagocytosis, Endocytosis, and Targeting","volume":"9","author":"Anselmo","year":"2015","journal-title":"ACS Nano"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1146\/annurev.immunol.15.1.235","article-title":"Neutralizing Antiviral B Cell Responses","volume":"15","author":"Bachmann","year":"1997","journal-title":"Annu. Rev. Immunol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/nature12202","article-title":"Self-Assembling Influenza Nanoparticle Vaccines Elicit Broadly Neutralizing H1N1 Antibodies","volume":"499","author":"Kanekiyo","year":"2013","journal-title":"Nature"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1073\/pnas.1112648109","article-title":"Enhancing Humoral Responses to a Malaria Antigen with Nanoparticle Vaccines That Expand Tfh Cells and Promote Germinal Center Induction","volume":"109","author":"Moon","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/s41578-019-0110-7","article-title":"Material Design for Lymph Node Drug Delivery","volume":"4","author":"Schudel","year":"2019","journal-title":"Nat. Rev. Mater."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1038\/nbt.3371","article-title":"In Vivo Characterization of the Physicochemical Properties of Polymer-Linked TLR Agonists That Enhance Vaccine Immunogenicity","volume":"33","author":"Lynn","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3912","DOI":"10.1021\/nn305466z","article-title":"pH-Responsive Nanoparticle Vaccines for Dual-Delivery of Antigens and Immunostimulatory Oligonucleotides","volume":"7","author":"Wilson","year":"2013","journal-title":"ACS Nano"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.jconrel.2015.12.032","article-title":"Materials for Non-Viral Intracellular Delivery of Messenger RNA Therapeutics","volume":"240","author":"Kauffman","year":"2016","journal-title":"J. Control Release"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1038\/nature18300","article-title":"Systemic RNA Delivery to Dendritic Cells Exploits Antiviral Defence for Cancer Immunotherapy","volume":"534","author":"Kranz","year":"2016","journal-title":"Nature"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Apostolopoulos, V., Bojarska, J., Chai, T.-T., Elnagdy, S., Kaczmarek, K., Matsoukas, J., New, R., Parang, K., Lopez, O.P., and Parhiz, H. (2021). A Global Review on Short Peptides: Frontiers and Perspectives. Molecules, 26.","DOI":"10.3390\/molecules26020430"},{"key":"ref_31","first-page":"163\u2013175","article-title":"Anti-PEG Antibodies: Properties, Formation, Testing and Role in Adverse Immune Reactions to PEGylated Nano-Biopharmaceuticals","volume":"154\u2013155","author":"Kozma","year":"2020","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Sun, L., Miao, Y., Jiang, D., and Liu, C. (2026). Tools for Antigen Delivery: From Traditional Nanocarriers and Biomimetic Platforms to Emerging Physical, Bioengineered and Computational Approaches. Vaccines, 14.","DOI":"10.3390\/vaccines14060516"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.actbio.2024.12.039","article-title":"Impact of Nanoparticle Properties on Immune Cell Interactions in the Lymph Node","volume":"193","author":"Farooq","year":"2025","journal-title":"Acta Biomater."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Pati, R., Shevtsov, M., and Sonawane, A. (2018). Nanoparticle Vaccines Against Infectious Diseases. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.02224"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Lozano, D., Larraga, V., Vallet-Reg\u00ed, M., and Manzano, M. (2023). An Overview of the Use of Nanoparticles in Vaccine Development. Nanomaterials, 13.","DOI":"10.3390\/nano13121828"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"114829","DOI":"10.1016\/j.addr.2023.114829","article-title":"Nanoparticle-Based Immunotherapeutics: From the Properties of Nanocores to the Differential Effects of Administration Routes","volume":"197","author":"Polo","year":"2023","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Eslami, M., Fadaee Dowlat, B., Yaghmayee, S., Habibian, A., Keshavarzi, S., Oksenych, V., and Naderian, R. (2025). Next-Generation Vaccine Platforms: Integrating Synthetic Biology, Nanotechnology, and Systems Immunology for Improved Immunogenicity. Vaccines, 13.","DOI":"10.3390\/vaccines13060588"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1038\/s41578-021-00358-0","article-title":"Lipid Nanoparticles for mRNA Delivery","volume":"6","author":"Hou","year":"2021","journal-title":"Nat. Rev. Mater."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1038\/nrd.2017.243","article-title":"mRNA Vaccines\u2014A New Era in Vaccinology","volume":"17","author":"Pardi","year":"2018","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"11109","DOI":"10.1021\/acs.chemrev.5b00109","article-title":"Synthetic Nanoparticles for Vaccines and Immunotherapy","volume":"115","author":"Irvine","year":"2015","journal-title":"Chem. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Mohsen, M.O., Gomes, A.C., Vogel, M., and Bachmann, M.F. (2018). Interaction of Viral Capsid-Derived Virus-Like Particles (VLPs) with the Innate Immune System. Vaccines, 6.","DOI":"10.3390\/vaccines6030037"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Cao, S., Ma, D., Ji, S., Zhou, M., and Zhu, S. (2024). Self-Assembled Ferritin Nanoparticles for Delivery of Antigens and Development of Vaccines: From Structure and Property to Applications. Molecules, 29.","DOI":"10.3390\/molecules29174221"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Kim, S.A., Kim, S., Kim, G.B., Goo, J., Kim, N., Lee, Y., Nam, G.-H., Lim, S., Kim, T., and Chang, K.H. (2022). A Multivalent Vaccine Based on Ferritin Nanocage Elicits Potent Protective Immune Responses against SARS-CoV-2 Mutations. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23116123"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/308457a0","article-title":"Iscom, a Novel Structure for Antigenic Presentation of Membrane Proteins from Enveloped Viruses","volume":"308","author":"Morein","year":"1984","journal-title":"Nature"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1586\/erv.11.25","article-title":"ISCOM Technology-Based Matrix MTM Adjuvant: Success in Future Vaccines Relies on Formulation","volume":"10","author":"Morein","year":"2011","journal-title":"Expert. Rev. Vaccines"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1007\/s00705-019-04273-2","article-title":"Adjuvant Activity of Multimolecular Complexes Based on Glycyrrhiza Glabra Saponins, Lipids, and Influenza Virus Glycoproteins","volume":"164","author":"Alexyuk","year":"2019","journal-title":"Arch. Virol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1007\/s00705-020-04619-1","article-title":"Effect of the Nanocapsulated Adjuvant Sapomax on the Expression of Some Immune Response Genes","volume":"165","author":"Turmagambetova","year":"2020","journal-title":"Arch. Virol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3817","DOI":"10.1007\/s00705-017-3560-5","article-title":"Adjuvant Activity of Saponins from Kazakhstani Plants on the Immune Responses to Subunit Influenza Vaccine","volume":"162","author":"Turmagambetova","year":"2017","journal-title":"Arch. Virol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2256","DOI":"10.1039\/C1CS15166E","article-title":"Gold Nanoparticles in Biomedical Applications: Recent Advances and Perspectives","volume":"41","author":"Dykman","year":"2012","journal-title":"Chem. Soc. Rev."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.nano.2013.09.011","article-title":"Gold Nanoparticle Mediated Cancer Immunotherapy","volume":"10","author":"Almeida","year":"2014","journal-title":"Nanomedicine"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1016\/j.addr.2008.03.012","article-title":"Mesoporous Silica Nanoparticles as Controlled Release Drug Delivery and Gene Transfection Carriers","volume":"60","author":"Slowing","year":"2008","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"11023","DOI":"10.1021\/acsnano.5b04378","article-title":"Polymer-Grafted Mesoporous Silica Nanoparticles as Ultrasound-Responsive Drug Carriers","volume":"9","author":"Paris","year":"2015","journal-title":"ACS Nano"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1021\/acs.molpharmaceut.7b01103","article-title":"Iron Oxide Nanoparticles-Based Vaccine Delivery for Cancer Treatment","volume":"15","author":"Zhao","year":"2018","journal-title":"Mol. Pharm."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"100262","DOI":"10.1016\/j.cobme.2021.100262","article-title":"Recent Advances in Carbon Nanomaterials for Biomedical Applications: A Review","volume":"17","author":"Riley","year":"2021","journal-title":"Curr. Opin. Biomed. Eng."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Nazarizadeh, A., Staudacher, A.H., Wittwer, N.L., Turnbull, T., Brown, M.P., and Kempson, I. (2022). Aluminium Nanoparticles as Efficient Adjuvants Compared to Their Microparticle Counterparts: Current Progress and Perspectives. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23094707"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.biomaterials.2016.07.005","article-title":"Dual Stimulation of Antigen Presenting Cells Using Carbon Nanotube-Based Vaccine Delivery System for Cancer Immunotherapy","volume":"104","author":"Hassan","year":"2016","journal-title":"Biomaterials"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Zare-Zardini, H., Saberian, E., Jen\u010da, A., Jen\u010da, A., Petr\u00e1\u0161ov\u00e1, A., and Jen\u010dov\u00e1, J. (2024). A Narrative Review on the Promising Potential of Graphene in Vaccine Design: Evaluating the Benefits and Drawbacks of Carbon Nanoplates in Nanovaccine Production. Vaccines, 12.","DOI":"10.3390\/vaccines12060660"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Gregory, A.E., Titball, R., and Williamson, D. (2013). Vaccine Delivery Using Nanoparticles. Front. Cell. Infect. Microbiol., 3.","DOI":"10.3389\/fcimb.2013.00013"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1038\/nmat4644","article-title":"Biomimetic Proteolipid Vesicles for Targeting Inflamed Tissues","volume":"15","author":"Molinaro","year":"2016","journal-title":"Nat. Mater."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1038\/nmat2960","article-title":"Interbilayer-Crosslinked Multilamellar Vesicles as Synthetic Vaccines for Potent Humoral and Cellular Immune Responses","volume":"10","author":"Moon","year":"2011","journal-title":"Nat. Mater."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"662","DOI":"10.3390\/vaccines3030662","article-title":"Nanoparticle Drug Delivery Systems Designed to Improve Cancer Vaccines and Immunotherapy","volume":"3","author":"Fan","year":"2015","journal-title":"Vaccines"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1172\/JCI81083","article-title":"Beyond Antigens and Adjuvants: Formulating Future Vaccines","volume":"126","author":"Moyer","year":"2016","journal-title":"J. Clin. Investig."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"6465","DOI":"10.1021\/acsnano.5b02153","article-title":"Polyelectrolyte Multilayers Assembled Entirely from Immune Signals on Gold Nanoparticle Templates Promote Antigen-Specific T Cell Response","volume":"9","author":"Zhang","year":"2015","journal-title":"ACS Nano"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Sabourian, P., Yazdani, G., Ashraf, S.S., Frounchi, M., Mashayekhan, S., Kiani, S., and Kakkar, A. (2020). Effect of Physico-Chemical Properties of Nanoparticles on Their Intracellular Uptake. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21218019"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1038\/nnano.2012.207","article-title":"Biomolecular Coronas Provide the Biological Identity of Nanosized Materials","volume":"7","author":"Monopoli","year":"2012","journal-title":"Nat. Nanotechnol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"81","DOI":"10.2217\/nnm.15.188","article-title":"The Impact of Nanoparticle Protein Corona on Cytotoxicity, Immunotoxicity and Target Drug Delivery","volume":"11","author":"Corbo","year":"2016","journal-title":"Nanomedicine"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"3137","DOI":"10.2147\/IJN.S129300","article-title":"Protein Corona: A New Approach for Nanomedicine Design","volume":"12","author":"Nguyen","year":"2017","journal-title":"Int. J. Nanomed."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"101463","DOI":"10.1016\/j.omtm.2025.101463","article-title":"Lipid Nanoparticles: Composition, Formulation, and Application","volume":"33","author":"Xu","year":"2025","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.jconrel.2012.01.043","article-title":"PLGA-Based Nanoparticles: An Overview of Biomedical Applications","volume":"161","author":"Danhier","year":"2012","journal-title":"J. Control Release"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.3390\/polym3031377","article-title":"Poly Lactic-Co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier","volume":"3","author":"Makadia","year":"2011","journal-title":"Polymers"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1016\/S0142-9612(00)00115-0","article-title":"The Manufacturing Techniques of Various Drug Loaded Biodegradable Poly(Lactide-Co-Glycolide) (PLGA) Devices","volume":"21","author":"Jain","year":"2000","journal-title":"Biomaterials"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.colsurfb.2009.09.001","article-title":"Biodegradable Polymeric Nanoparticles Based Drug Delivery Systems","volume":"75","author":"Kumari","year":"2010","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1021\/js9900654","article-title":"Protein Behavior at the Water\/Methylene Chloride Interface","volume":"88","author":"Sah","year":"1999","journal-title":"J. Pharm. Sci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1163\/156856206775997322","article-title":"Synthesis and Characterization of PLGA Nanoparticles","volume":"17","author":"Astete","year":"2006","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1002\/jps.21082","article-title":"Engineering Biodegradable Polyester Particles with Specific Drug Targeting and Drug Release Properties","volume":"97","author":"Mohamed","year":"2008","journal-title":"J. Pharm. Sci."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s13205-014-0214-0","article-title":"Nanoemulsion: An Advanced Mode of Drug Delivery System","volume":"5","author":"Jaiswal","year":"2015","journal-title":"3 Biotech"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"e70025","DOI":"10.1002\/wnan.70025","article-title":"Emerging Biomimetic Drug Delivery Nanoparticles Inspired by Extracellular Vesicles","volume":"17","author":"Sundaram","year":"2025","journal-title":"Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1042\/bj20031253","article-title":"Size-Dependent Internalization of Particles via the Pathways of Clathrin- and Caveolae-Mediated Endocytosis","volume":"377","author":"Rejman","year":"2004","journal-title":"Biochem. J."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1089\/nat.2018.0721","article-title":"Lipid Nanoparticles Enabling Gene Therapies: From Concepts to Clinical Utility","volume":"28","author":"Kulkarni","year":"2018","journal-title":"Nucleic Acid. Ther."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1016\/j.ymthe.2017.03.013","article-title":"Lipid Nanoparticle Systems for Enabling Gene Therapies","volume":"25","author":"Cullis","year":"2017","journal-title":"Mol. Ther."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.jconrel.2011.06.001","article-title":"Targeted Drug Delivery to Tumors: Myths, Reality and Possibility","volume":"153","author":"Bae","year":"2011","journal-title":"J. Control Release"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"44826","DOI":"10.1074\/jbc.M308643200","article-title":"Chloride Accumulation and Swelling in Endosomes Enhances DNA Transfer by Polyamine-DNA Polyplexes","volume":"278","author":"Sonawane","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1586\/14760584.2015.986104","article-title":"Lipid-Based mRNA Vaccine Delivery Systems","volume":"14","author":"Midoux","year":"2015","journal-title":"Expert. Rev. Vaccines"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"4341","DOI":"10.1016\/j.vaccine.2011.09.061","article-title":"The Mechanism of Action of MF59\u2014An Innately Attractive Adjuvant Formulation","volume":"30","author":"Ott","year":"2012","journal-title":"Vaccine"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"3363","DOI":"10.1016\/j.vaccine.2013.05.007","article-title":"The Adjuvant Effect of MF59 Is Due to the Oil-in-Water Emulsion Formulation, None of the Individual Components Induce a Comparable Adjuvant Effect","volume":"31","author":"Calabro","year":"2013","journal-title":"Vaccine"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"11169","DOI":"10.1073\/pnas.1107941108","article-title":"Adjuvanticity of the Oil-in-Water Emulsion MF59 Is Independent of Nlrp3 Inflammasome but Requires the Adaptor Protein MyD88","volume":"108","author":"Seubert","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"27066","DOI":"10.3402\/jev.v4.27066","article-title":"Biological Properties of Extracellular Vesicles and Their Physiological Functions","volume":"4","author":"Siljander","year":"2015","journal-title":"J. Extracell. Vesicles"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"eaau6977","DOI":"10.1126\/science.aau6977","article-title":"The Biology, Function, and Biomedical Applications of Exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"26316","DOI":"10.3402\/jev.v4.26316","article-title":"Extracellular Vesicle in Vivo Biodistribution Is Determined by Cell Source, Route of Administration and Targeting","volume":"4","author":"Wiklander","year":"2015","journal-title":"J. Extracell. Vesicles"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/nbt.1807","article-title":"Delivery of siRNA to the Mouse Brain by Systemic Injection of Targeted Exosomes","volume":"29","author":"Seow","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1038\/nri3488","article-title":"Applications of Nanotechnology for Immunology","volume":"13","author":"Smith","year":"2013","journal-title":"Nat. Rev. Immunol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.immuni.2005.06.008","article-title":"Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modification and the Evolutionary Origin of RNA","volume":"23","author":"Buckstein","year":"2005","journal-title":"Immunity"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1016\/j.addr.2012.05.009","article-title":"Cationic Lipids Activate Intracellular Signaling Pathways","volume":"64","author":"Lonez","year":"2012","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"103479","DOI":"10.1016\/j.isci.2021.103479","article-title":"The mRNA-LNP Platform\u2019s Lipid Nanoparticle Component Used in Preclinical Vaccine Studies Is Highly Inflammatory","volume":"24","author":"Ndeupen","year":"2021","journal-title":"iScience"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1073\/pnas.0804897106","article-title":"Uptake of Particulate Vaccine Adjuvants by Dendritic Cells Activates the NALP3 Inflammasome","volume":"106","author":"Sharp","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1038\/ni.1631","article-title":"Silica Crystals and Aluminum Salts Activate the NALP3 Inflammasome through Phagosomal Destabilization","volume":"9","author":"Hornung","year":"2008","journal-title":"Nat. Immunol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1146\/annurev-biochem-013118-111902","article-title":"Exosomes","volume":"88","author":"Pegtel","year":"2019","journal-title":"Annu. Rev. Biochem."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"443","DOI":"10.2174\/156720105774370212","article-title":"Complement Activation-Related Pseudoallergy Caused by Amphiphilic Drug Carriers: The Role of Lipoproteins","volume":"2","author":"Szebeni","year":"2005","journal-title":"Curr. Drug Deliv."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1038\/s41565-019-0591-y","article-title":"The Onpattro Story and the Clinical Translation of Nanomedicines Containing Nucleic Acid-Based Drugs","volume":"14","author":"Akinc","year":"2019","journal-title":"Nat. Nanotechnol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"100766","DOI":"10.1016\/j.nantod.2019.100766","article-title":"Three Decades of Messenger RNA Vaccine Development","volume":"28","author":"Verbeke","year":"2019","journal-title":"Nano Today"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1586\/erv.11.192","article-title":"Development and Evaluation of AS03, an Adjuvant System Containing \u03b1-Tocopherol and Squalene in an Oil-in-Water Emulsion","volume":"11","author":"Vaughn","year":"2012","journal-title":"Expert. Rev. Vaccines"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"3286","DOI":"10.3390\/molecules14093286","article-title":"Squalene Emulsions for Parenteral Vaccine and Drug Delivery","volume":"14","author":"Fox","year":"2009","journal-title":"Molecules"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.jconrel.2014.06.027","article-title":"Designing and Building the next Generation of Improved Vaccine Adjuvants","volume":"190","author":"Brito","year":"2014","journal-title":"J. Control Release"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"e1706759","DOI":"10.1002\/adma.201706759","article-title":"Cell Membrane Coating Nanotechnology","volume":"30","author":"Fang","year":"2018","journal-title":"Adv. Mater."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1016\/S1359-6446(03)02864-2","article-title":"Vaccine Adjuvants: Role and Mechanisms of Action in Vaccine Immunogenicity","volume":"8","author":"Marciani","year":"2003","journal-title":"Drug Discov. Today"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1016\/j.vaccine.2009.01.091","article-title":"Advances in Saponin-Based Adjuvants","volume":"27","author":"Sun","year":"2009","journal-title":"Vaccine"},{"key":"ref_107","first-page":"1","article-title":"Saponins as Vaccine Adjuvants","volume":"13","author":"Kensil","year":"1996","journal-title":"Crit. Rev. Ther. Drug Carr. Syst."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.addr.2004.09.006","article-title":"ISCOMATRIX Adjuvant for Antigen Delivery","volume":"57","author":"Pearse","year":"2005","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.1016\/j.vaccine.2016.02.033","article-title":"Matrix-M Adjuvant Enhances Antibody, Cellular and Protective Immune Responses of a Zaire Ebola\/Makona Virus Glycoprotein (GP) Nanoparticle Vaccine in Mice","volume":"34","author":"Bengtsson","year":"2016","journal-title":"Vaccine"},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Welsby, I., Detienne, S., N\u2019Kuli, F., Thomas, S., Wouters, S., Bechtold, V., De Wit, D., Gineste, R., Reinheckel, T., and Elouahabi, A. (2016). Lysosome-Dependent Activation of Human Dendritic Cells by the Vaccine Adjuvant QS-21. Front. Immunol., 7.","DOI":"10.3389\/fimmu.2016.00663"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/0167-5699(87)90008-9","article-title":"The ISCOM: An Immunostimulating Complex","volume":"8","author":"Morein","year":"1987","journal-title":"Immunol. Today"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"2189885","DOI":"10.1080\/21645515.2023.2189885","article-title":"The Matrix-MTM Adjuvant: A Critical Component of Vaccines for the 21st Century","volume":"19","author":"Stertman","year":"2023","journal-title":"Hum. Vaccin. Immunother."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1038\/s41541-021-00369-6","article-title":"Distinguishing Features of Current COVID-19 Vaccines: Knowns and Unknowns of Antigen Presentation and Modes of Action","volume":"6","author":"Heinz","year":"2021","journal-title":"npj Vaccines"},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1631\/jzus.2007.B0153","article-title":"Adjuvant Effects of Saponins on Animal Immune Responses","volume":"8","author":"Rajput","year":"2007","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Jiang, M., Hong, C., Zou, W., Ye, Z., Lu, L., Liu, Y., Zhang, T., and Ding, Y. (2024). Recent Advances in the Anti-Tumor Activities of Saponins through Cholesterol Regulation. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1469392"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1586\/erv.10.154","article-title":"Combination Adjuvants: The next Generation of Adjuvants?","volume":"10","author":"Mutwiri","year":"2011","journal-title":"Expert Rev. Vaccines"},{"key":"ref_117","doi-asserted-by":"crossref","unstructured":"Verstraeten, S.L., Lorent, J.H., and Mingeot-Leclercq, M.-P. (2020). Lipid Membranes as Key Targets for the Pharmacological Actions of Ginsenosides. Front. Pharmacol., 11.","DOI":"10.3389\/fphar.2020.576887"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"2743","DOI":"10.1080\/10717544.2022.2112997","article-title":"A Novel Saponin Liposomes Based on the Couplet Medicines of Platycodon Grandiflorum-Glycyrrhiza Uralensis for Targeting Lung Cancer","volume":"29","author":"Guo","year":"2022","journal-title":"Drug Deliv."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1016\/j.vaccine.2003.12.031","article-title":"ISCOMATRIX Adjuvant: A Potent Inducer of Humoral and Cellular Immune Responses","volume":"22","author":"Pearse","year":"2004","journal-title":"Vaccine"},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.tips.2018.03.005","article-title":"Elucidating the Mechanisms of Action of Saponin-Derived Adjuvants","volume":"39","author":"Marciani","year":"2018","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_121","doi-asserted-by":"crossref","unstructured":"Talukdar, P., Winegar, P.H., Hudson, G.A., Astolfi, M.C.T., Inman, J.L., Keasling, J.D., and Mukundan, H. (2025). From Bark to Bench: Innovations in QS-21 Adjuvant Characterization and Manufacturing. Front. Immunol., 16.","DOI":"10.3389\/fimmu.2025.1677995"},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"118223","DOI":"10.1016\/j.ejmech.2025.118223","article-title":"QS-21: Structural Insights, Immunological Mechanisms, and Prospects for Rational Design of Safer Vaccine Adjuvants","volume":"301","author":"Hu","year":"2026","journal-title":"Eur. J. Med. Chem."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1007\/s12026-018-8991-x","article-title":"Matrix-MTM Adjuvant Enhances Immunogenicity of Both Protein- and Modified Vaccinia Virus Ankara-Based Influenza Vaccines in Mice","volume":"66","author":"Magnusson","year":"2018","journal-title":"Immunol. Res."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1007\/s00044-024-03227-x","article-title":"Development of Semisynthetic Saponin Immunostimulants","volume":"33","author":"Bai","year":"2024","journal-title":"Med. Chem. Res."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1007\/s12033-012-9598-4","article-title":"Construction and Characterization of Virus-like Particles: A Review","volume":"53","author":"Zeltins","year":"2013","journal-title":"Mol. Biotechnol."},{"key":"ref_126","doi-asserted-by":"crossref","unstructured":"Brune, K.D., and Howarth, M. (2018). New Routes and Opportunities for Modular Construction of Particulate Vaccines: Stick, Click, and Glue. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.01432"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.csbj.2015.11.001","article-title":"Self-Assembling Protein Nanoparticles in the Design of Vaccines","volume":"14","author":"Malito","year":"2016","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1080\/14760584.2019.1578216","article-title":"Immunological Basis for Enhanced Immunity of Nanoparticle Vaccines","volume":"18","author":"Kelly","year":"2019","journal-title":"Expert. Rev. Vaccines"},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1016\/j.cell.2021.01.035","article-title":"Two-Component Spike Nanoparticle Vaccine Protects Macaques from SARS-CoV-2 Infection","volume":"184","author":"Brouwer","year":"2021","journal-title":"Cell"},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1002\/bit.25159","article-title":"Bioengineering Virus-like Particles as Vaccines","volume":"111","author":"Lua","year":"2014","journal-title":"Biotechnol. Bioeng."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.vaccine.2012.10.083","article-title":"Virus-like Particles as a Highly Efficient Vaccine Platform: Diversity of Targets and Production Systems and Advances in Clinical Development","volume":"31","author":"Kushnir","year":"2012","journal-title":"Vaccine"},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"12041","DOI":"10.1038\/ncomms12041","article-title":"Presenting Native-like Trimeric HIV-1 Antigens with Self-Assembling Nanoparticles","volume":"7","author":"He","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s12951-021-00806-7","article-title":"Virus-like Particles: Preparation, Immunogenicity and Their Roles as Nanovaccines and Drug Nanocarriers","volume":"19","author":"Nooraei","year":"2021","journal-title":"J. Nanobiotechnol."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1021\/acscentsci.0c01405","article-title":"A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice","volume":"7","author":"Powell","year":"2021","journal-title":"ACS Cent. Sci."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"130288","DOI":"10.1016\/j.bbagen.2022.130288","article-title":"Ferritin Nanocages as Efficient Nanocarriers and Promising Platforms for COVID-19 and Other Vaccines Development","volume":"1867","author":"Reutovich","year":"2023","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/nature13404","article-title":"Accurate Design of Co-Assembling Multi-Component Protein Nanomaterials","volume":"510","author":"King","year":"2014","journal-title":"Nature"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1016\/j.cell.2020.10.043","article-title":"Elicitation of Potent Neutralizing Antibody Responses by Designed Protein Nanoparticle Vaccines for SARS-CoV-2","volume":"183","author":"Walls","year":"2020","journal-title":"Cell"},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"20220867","DOI":"10.1515\/biol-2022-0867","article-title":"Mesoporous Silica Nanoparticles as a Drug Delivery Mechanism","volume":"19","author":"Zhang","year":"2024","journal-title":"Open Life Sci."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"126192","DOI":"10.1016\/j.ijpharm.2025.126192","article-title":"Smart Inorganic Nanoparticles in Nanomedicine: Strategies for Synthesis and Functionalization","volume":"684","author":"Percoco","year":"2025","journal-title":"Int. J. Pharm."},{"key":"ref_140","doi-asserted-by":"crossref","unstructured":"Xia, C., Luo, Z., Feng, Z., Zhang, Q., and Xia, C. (2025). Mechanism and Application of Copper-Based Nanomedicines in Activating Tumor Immunity through Oxidative Stress Modulation. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1646890"},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.1039\/C1CS15237H","article-title":"The Golden Age: Gold Nanoparticles for Biomedicine","volume":"41","author":"Dreaden","year":"2012","journal-title":"Chem. Soc. Rev."},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.addr.2012.07.018","article-title":"Mesoporous Silica Nanoparticles in Medicine--Recent Advances","volume":"65","author":"Mamaeva","year":"2013","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_143","first-page":"H217","article-title":"Cancer Nanotheranostics: Improving Imaging and Therapy by Targeted Delivery across Biological Barriers","volume":"23","author":"Kievit","year":"2011","journal-title":"Adv. Mater."},{"key":"ref_144","doi-asserted-by":"crossref","unstructured":"Lin, X., and Huang, Q. (2023). Editorial: Smart Nanomaterials for Biosensing and Therapy Applications. Front. Bioeng. Biotechnol., 11.","DOI":"10.3389\/fbioe.2023.1137508"},{"key":"ref_145","doi-asserted-by":"crossref","unstructured":"Nguyen, M.D., Hoijang, S., Yarinia, R., Ariza Gonzalez, M., Mandal, S., Tran, Q.M., Chinwangso, P., and Lee, T.R. (2025). Magnetic Iron Oxide Nanoparticles: Advances in Synthesis, Mechanistic Understanding, and Magnetic Property Optimization for Improved Biomedical Performance. Nanomaterials, 15.","DOI":"10.3390\/nano15191500"},{"key":"ref_146","doi-asserted-by":"crossref","unstructured":"Mahor, A., Singh, P.P., Bharadwaj, P., Sharma, N., Yadav, S., Rosenholm, J.M., and Bansal, K.K. (2021). Carbon-Based Nanomaterials for Delivery of Biologicals and Therapeutics: A Cutting-Edge Technology. C, 7.","DOI":"10.3390\/c7010019"},{"key":"ref_147","doi-asserted-by":"crossref","unstructured":"Yang, R., Liu, Z., Chen, H., Zhang, X., Sun, Q., El-Mesery, H.S., Lu, W., Dai, X., and Xu, R. (2025). Advances in Nanozyme Catalysis for Food Safety Detection: A Comprehensive Review on Progress and Challenges. Foods, 14.","DOI":"10.3390\/foods14152580"},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"2050","DOI":"10.1073\/pnas.0608582104","article-title":"Understanding the Nanoparticle-Protein Corona Using Methods to Quantify Exchange Rates and Affinities of Proteins for Nanoparticles","volume":"104","author":"Cedervall","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev-bioeng-071811-150124","article-title":"The Effect of Nanoparticle Size, Shape, and Surface Chemistry on Biological Systems","volume":"14","author":"Albanese","year":"2012","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.jconrel.2011.09.098","article-title":"The Journey of a Drug-Carrier in the Body: An Anatomo-Physiological Perspective","volume":"161","author":"Bertrand","year":"2012","journal-title":"J. Control Release"},{"key":"ref_151","doi-asserted-by":"crossref","unstructured":"Graham, W., Torbett-Dougherty, M., Islam, A., Soleimani, S., Bruce-Tagoe, T.A., and Johnson, J.A. (2025). Magnetic Nanoparticles and Drug Delivery Systems for Anti-Cancer Applications: A Review. Nanomaterials, 15.","DOI":"10.3390\/nano15040285"},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"e1449","DOI":"10.1002\/wnan.1449","article-title":"Gold Nanoparticle-Mediated Photothermal Therapy: Applications and Opportunities for Multimodal Cancer Treatment","volume":"9","author":"Riley","year":"2017","journal-title":"Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol."},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"1700996","DOI":"10.1002\/adma.201700996","article-title":"Emerging Nanotechnology and Advanced Materials for Cancer Radiation Therapy","volume":"29","author":"Song","year":"2017","journal-title":"Adv. Mater."},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"e42738","DOI":"10.1016\/j.heliyon.2025.e42738","article-title":"Utilizing Gold Nanoparticles in Plasmonic Photothermal Therapy for Cancer Treatment","volume":"11","author":"Badir","year":"2025","journal-title":"Heliyon"},{"key":"ref_155","doi-asserted-by":"crossref","first-page":"2401","DOI":"10.1016\/j.fmre.2024.08.006","article-title":"Heating up the Immune Battle: Magnetic Hyperthermia against Cancer","volume":"5","author":"Jiao","year":"2025","journal-title":"Fundam. Res."},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"102540","DOI":"10.1016\/j.mtchem.2025.102540","article-title":"Regulator of Oxidative Balance: Research Progress of Nanozymes in ROS-Related Diseases","volume":"44","author":"Wang","year":"2025","journal-title":"Mater. Today Chem."},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"3965","DOI":"10.1021\/ja2108905","article-title":"Nanoparticle Hydrophobicity Dictates Immune Response","volume":"134","author":"Moyano","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"ref_158","doi-asserted-by":"crossref","first-page":"5717","DOI":"10.1021\/nn2013904","article-title":"The Impact of Silica Nanoparticle Design on Cellular Toxicity and Hemolytic Activity","volume":"5","author":"Yu","year":"2011","journal-title":"ACS Nano"},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1038\/nnano.2016.168","article-title":"Iron Oxide Nanoparticles Inhibit Tumour Growth by Inducing Pro-Inflammatory Macrophage Polarization in Tumour Tissues","volume":"11","author":"Zanganeh","year":"2016","journal-title":"Nat. Nanotechnol."},{"key":"ref_160","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Fern\u00e1ndez, S., Blanco-Agud\u00edn, N., Rodr\u00edguez, D., Fern\u00e1ndez-Vega, I., Merayo-Lloves, J., and Quir\u00f3s, L.M. (2025). Silver Nanoparticles: A Versatile Tool Against Infectious and Non-Infectious Diseases. Antibiotics, 14.","DOI":"10.3390\/antibiotics14030289"},{"key":"ref_161","doi-asserted-by":"crossref","first-page":"2400380","DOI":"10.1002\/gch2.202400380","article-title":"Silver Nanoparticle-Mediated Antiviral Efficacy against Enveloped Viruses: A Comprehensive Review","volume":"9","author":"Mosidze","year":"2025","journal-title":"Glob. Chall."},{"key":"ref_162","doi-asserted-by":"crossref","unstructured":"Svadlakova, T., Holmannova, D., Kolackova, M., Malkova, A., Krejsek, J., and Fiala, Z. (2022). Immunotoxicity of Carbon-Based Nanomaterials, Starring Phagocytes. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23168889"},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"042005","DOI":"10.1088\/1748-605X\/abe5fa","article-title":"Biocompatibility of Nanomaterials and Their Immunological Properties","volume":"16","author":"Kyriakides","year":"2021","journal-title":"Biomed. Mater."},{"key":"ref_164","doi-asserted-by":"crossref","first-page":"16014","DOI":"10.1038\/natrevmats.2016.14","article-title":"Analysis of Nanoparticle Delivery to Tumours","volume":"1","author":"Wilhelm","year":"2016","journal-title":"Nat. Rev. Mater."},{"key":"ref_165","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1038\/nbt.3330","article-title":"Principles of Nanoparticle Design for Overcoming Biological Barriers to Drug Delivery","volume":"33","author":"Blanco","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_166","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/s41591-020-0753-3","article-title":"Engineered Immunogen Binding to Alum Adjuvant Enhances Humoral Immunity","volume":"26","author":"Moyer","year":"2020","journal-title":"Nat. Med."},{"key":"ref_167","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.addr.2020.06.019","article-title":"Controlling Timing and Location in Vaccines","volume":"158","author":"Irvine","year":"2020","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_168","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1038\/s41565-020-00790-3","article-title":"Eliciting B Cell Immunity against Infectious Diseases Using Nanovaccines","volume":"16","author":"Singh","year":"2021","journal-title":"Nat. Nanotechnol."},{"key":"ref_169","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1038\/s41423-025-01316-4","article-title":"Eliciting Antitumor Immunity via Therapeutic Cancer Vaccines","volume":"22","author":"Peng","year":"2025","journal-title":"Cell. Mol. Immunol."},{"key":"ref_170","doi-asserted-by":"crossref","unstructured":"Elfatimi, E., Lekbach, Y., Prakash, S., and BenMohamed, L. (2025). Artificial Intelligence and Machine Learning in the Development of Vaccines and Immunotherapeutics-Yesterday, Today, and Tomorrow. Front. Artif. Intell., 8.","DOI":"10.3389\/frai.2025.1620572"},{"key":"ref_171","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1038\/s41573-021-00163-y","article-title":"Emerging Concepts in the Science of Vaccine Adjuvants","volume":"20","author":"Pulendran","year":"2021","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_172","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.coi.2017.06.008","article-title":"Germinal Center Enhancement by Extended Antigen Availability","volume":"47","author":"Cirelli","year":"2017","journal-title":"Curr. Opin. Immunol."},{"key":"ref_173","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1038\/nri3727","article-title":"Dendritic Cell-Targeted Vaccines--Hope or Hype?","volume":"14","author":"Kurts","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref_174","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/s41568-019-0186-9","article-title":"Improving Cancer Immunotherapy through Nanotechnology","volume":"19","author":"Goldberg","year":"2019","journal-title":"Nat. Rev. Cancer"},{"key":"ref_175","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.addr.2012.10.003","article-title":"Advanced Materials and Processing for Drug Delivery: The Past and the Future","volume":"65","author":"Zhang","year":"2013","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_176","doi-asserted-by":"crossref","first-page":"5483","DOI":"10.1002\/smll.201501284","article-title":"Nanoparticle-Based Manipulation of Antigen-Presenting Cells for Cancer Immunotherapy","volume":"11","author":"Fang","year":"2015","journal-title":"Small"},{"key":"ref_177","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.1007\/s11095-014-1419-y","article-title":"Biomaterials for Nanoparticle Vaccine Delivery Systems","volume":"31","author":"Sahdev","year":"2014","journal-title":"Pharm. Res."},{"key":"ref_178","doi-asserted-by":"crossref","first-page":"16071","DOI":"10.1038\/natrevmats.2016.71","article-title":"Designing Hydrogels for Controlled Drug Delivery","volume":"1","author":"Li","year":"2016","journal-title":"Nat. Rev. Mater."},{"key":"ref_179","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1021\/acs.nanolett.5b05030","article-title":"Enhanced Cancer Immunotherapy by Microneedle Patch-Assisted Delivery of Anti-PD1 Antibody","volume":"16","author":"Wang","year":"2016","journal-title":"Nano Lett."},{"key":"ref_180","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.addr.2013.09.006","article-title":"Multifunctional Nanoparticles for Brain Tumor Imaging and Therapy","volume":"66","author":"Cheng","year":"2014","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_181","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly Accurate Protein Structure Prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"ref_182","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1038\/s41586-021-03365-x","article-title":"Quadrivalent Influenza Nanoparticle Vaccines Induce Broad Protection","volume":"592","author":"Ellis","year":"2021","journal-title":"Nature"},{"key":"ref_183","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1126\/science.1219364","article-title":"Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy","volume":"336","author":"King","year":"2012","journal-title":"Science"},{"key":"ref_184","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1126\/science.aar7112","article-title":"Personalized Vaccines for Cancer Immunotherapy","volume":"359","author":"Sahin","year":"2018","journal-title":"Science"},{"key":"ref_185","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1038\/s41586-018-0792-9","article-title":"Neoantigen Vaccine Generates Intratumoral T Cell Responses in Phase Ib Glioblastoma Trial","volume":"565","author":"Keskin","year":"2019","journal-title":"Nature"},{"key":"ref_186","doi-asserted-by":"crossref","first-page":"eaay4014","DOI":"10.1126\/science.aay4014","article-title":"The Science and Medicine of Human Immunology","volume":"369","author":"Pulendran","year":"2020","journal-title":"Science"},{"key":"ref_187","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1038\/s41573-019-0024-5","article-title":"Applications of Machine Learning in Drug Discovery and Development","volume":"18","author":"Vamathevan","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_188","first-page":"218590","article-title":"Emerging Vaccine Informatics","volume":"2010","author":"He","year":"2010","journal-title":"J. Biomed. Biotechnol."},{"key":"ref_189","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1038\/s41573-020-0090-8","article-title":"Engineering Precision Nanoparticles for Drug Delivery","volume":"20","author":"Mitchell","year":"2021","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_190","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1002\/btm2.10003","article-title":"Nanoparticles in the Clinic","volume":"1","author":"Anselmo","year":"2016","journal-title":"Bioeng. Transl. Med."},{"key":"ref_191","first-page":"582","article-title":"The Nanomedicine Revolution","volume":"37","author":"Ventola","year":"2012","journal-title":"Pharm. Ther."},{"key":"ref_192","doi-asserted-by":"crossref","first-page":"7442","DOI":"10.1021\/nn404501g","article-title":"Facing the Truth about Nanotechnology in Drug Delivery","volume":"7","author":"Park","year":"2013","journal-title":"ACS Nano"},{"key":"ref_193","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.nano.2012.05.013","article-title":"The Big Picture on Nanomedicine: The State of Investigational and Approved Nanomedicine Products","volume":"9","author":"Etheridge","year":"2013","journal-title":"Nanomedicine"},{"key":"ref_194","doi-asserted-by":"crossref","first-page":"120826","DOI":"10.1016\/j.biomaterials.2021.120826","article-title":"Microfluidic Formulation of Nanoparticles for Biomedical Applications","volume":"274","author":"Shepherd","year":"2021","journal-title":"Biomaterials"},{"key":"ref_195","doi-asserted-by":"crossref","first-page":"119266","DOI":"10.1016\/j.ijpharm.2020.119266","article-title":"Using Microfluidics for Scalable Manufacturing of Nanomedicines from Bench to GMP: A Case Study Using Protein-Loaded Liposomes","volume":"582","author":"Webb","year":"2020","journal-title":"Int. J. Pharm."},{"key":"ref_196","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1038\/s41565-018-0246-4","article-title":"Minimum Information Reporting in Bio-Nano Experimental Literature","volume":"13","author":"Faria","year":"2018","journal-title":"Nat. Nanotechnol."},{"key":"ref_197","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1039\/c2ib20117h","article-title":"Common Pitfalls in Nanotechnology: Lessons Learned from NCI\u2019s Nanotechnology Characterization Laboratory","volume":"5","author":"Crist","year":"2013","journal-title":"Integr. Biol."},{"key":"ref_198","doi-asserted-by":"crossref","first-page":"2373","DOI":"10.1007\/s11095-016-1958-5","article-title":"Nanoparticle-Based Medicines: A Review of FDA-Approved Materials and Clinical Trials to Date","volume":"33","author":"Bobo","year":"2016","journal-title":"Pharm. Res."},{"key":"ref_199","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.jconrel.2013.05.025","article-title":"Understanding the Correlation between in Vitro and in Vivo Immunotoxicity Tests for Nanomedicines","volume":"172","author":"Dobrovolskaia","year":"2013","journal-title":"J. Control Release"},{"key":"ref_200","doi-asserted-by":"crossref","first-page":"a028894","DOI":"10.1101\/cshperspect.a028894","article-title":"Will Systems Biology Deliver Its Promise and Contribute to the Development of New or Improved Vaccines? From Data to Understanding through Systems Biology","volume":"10","author":"Hagan","year":"2018","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_201","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1038\/nri2629","article-title":"Learning Immunology from the Yellow Fever Vaccine: Innate Immunity to Systems Vaccinology","volume":"9","author":"Pulendran","year":"2009","journal-title":"Nat. Rev. Immunol."},{"key":"ref_202","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.addr.2016.04.028","article-title":"Toxicology of Graphene-Based Nanomaterials","volume":"105","author":"Lalwani","year":"2016","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_203","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1038\/nature22991","article-title":"An Immunogenic Personal Neoantigen Vaccine for Patients with Melanoma","volume":"547","author":"Ott","year":"2017","journal-title":"Nature"},{"key":"ref_204","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1038\/nature23003","article-title":"Personalized RNA Mutanome Vaccines Mobilize Poly-Specific Therapeutic Immunity against Cancer","volume":"547","author":"Sahin","year":"2017","journal-title":"Nature"},{"key":"ref_205","doi-asserted-by":"crossref","first-page":"e10060","DOI":"10.1002\/amp2.10060","article-title":"Rapid Development and Deployment of High-Volume Vaccines for Pandemic Response","volume":"2","author":"Kis","year":"2020","journal-title":"J. Adv. Manuf. Process"},{"key":"ref_206","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/j.xphs.2020.12.006","article-title":"Addressing the Cold Reality of mRNA Vaccine Stability","volume":"110","author":"Crommelin","year":"2021","journal-title":"J. Pharm. Sci."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/14\/2501\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T02:33:36Z","timestamp":1784514816000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/14\/2501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,17]]},"references-count":206,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["molecules31142501"],"URL":"https:\/\/doi.org\/10.3390\/molecules31142501","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,17]]}}}