{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T06:02:34Z","timestamp":1785132154622,"version":"3.55.0"},"reference-count":36,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Sulforaphane (SF), the renowned hydrolysis product of glucoraphanin from broccoli, is known to undergo thermal degradation to 3-butenyl isothiocyanate (3-BITC) during gas chromatography\u2013mass spectrometry (GC-MS) analysis. In this study, the effects on SF degradation associated with exposure to oven temperature during column transit, passage through the split\/splitless injector, and through the transfer line to the mass spectrometer were investigated. Massive degradation was observed during column transit when the oven temperature exceeded 150 \u00b0C. Degradation during the passage through the injector increased with temperature, from 150 \u00b0C to 250 \u00b0C, and with decreasing split ratio, from 100:1 to 20:1. Conventional splitless conditions with the injector temperature set at 250 \u00b0C resulted in a degradation &gt; 60%. Operating the GC-MS system with oven temperature below 150 \u00b0C prior to SF elution, an injector at 225 \u00b0C and a split ratio of 20:1, SF degradation was reduced to less than 1%. Under these optimized analytical conditions, the concentration of free SF in two broccoli-based dietary supplements was estimated based on external calibration and using 2,6-dimethylphenyl isothiocyanate as an internal standard. The GC-MS method proved to be a valuable tool for the quality evaluation of SF-labeled supplements integrated with the official EU ISO 9167:2019 HPLC-PDA glucosinolates analysis.<\/jats:p>","DOI":"10.3390\/molecules31152598","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:49:27Z","timestamp":1785131367000},"page":"2598","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sulforaphane Determination by GC-MS Analysis: Mitigation of Thermal Degradation and Quality Evaluation of Two Broccoli-Based Supplements"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9071-5680","authenticated-orcid":false,"given":"Valentina","family":"Melini","sequence":"first","affiliation":[{"name":"Consiglio per la Ricerca in Agricoltura e l\u2019Analisi dell\u2019Economia Agraria (CREA), Centro di Ricerca Alimenti e Nutrizione, Via Ardeatina, 546, 00178 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3282-1742","authenticated-orcid":false,"given":"Irene","family":"Baiamonte","sequence":"additional","affiliation":[{"name":"Consiglio per la Ricerca in Agricoltura e l\u2019Analisi dell\u2019Economia Agraria (CREA), Centro di Ricerca Alimenti e Nutrizione, Via Ardeatina, 546, 00178 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7465-0172","authenticated-orcid":false,"given":"Francesca","family":"Masciola","sequence":"additional","affiliation":[{"name":"Consiglio per la Ricerca in Agricoltura e l\u2019Analisi dell\u2019Economia Agraria (CREA), Centro di Ricerca Alimenti e Nutrizione, Via Ardeatina, 546, 00178 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8507-2374","authenticated-orcid":false,"given":"Antonio","family":"Raffo","sequence":"additional","affiliation":[{"name":"Consiglio per la Ricerca in Agricoltura e l\u2019Analisi dell\u2019Economia Agraria (CREA), Centro di Ricerca Alimenti e Nutrizione, Via Ardeatina, 546, 00178 Roma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2916-8662","authenticated-orcid":false,"given":"Gina Rosalinda","family":"De Nicola","sequence":"additional","affiliation":[{"name":"Consiglio per la Ricerca in Agricoltura e l\u2019Analisi dell\u2019Economia Agraria (CREA), Centro di Ricerca Orticoltura e Florovivaismo, Via dei Fiori, 8, 51017 Pescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5440","DOI":"10.1002\/ptr.7176","article-title":"Sulforaphane: A review of its therapeutic potentials, advances in its nanodelivery, recent patents, and clinical trials","volume":"35","author":"Mangla","year":"2021","journal-title":"Phytother. 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