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In our previous work, ginseng root and horse chestnut seed extracts were identified as potent stimulators of pancreatic lipase. Here, we expanded this investigation to additional Panax and Aesculus species and to saponin-rich extracts from Acer pseudoplatanus, Herniaria glabra, and Polypodium vulgare. Extracts were evaluated for effects on pancreatin lipolysis in relation to equilibrium surface tension, surface rheology, and olive-oil emulsion stability. Antiradical and antioxidant activities, total phenolic content, and flavonoid content were also determined. White ginseng root extract (Panax ginseng) produced the strongest stimulation, followed by horse chestnut seed extract (Aesculus hippocastanum), whereas Aesculus marylandica seed peel extract inhibited lipase activity. Several extracts showed concentration-dependent switching from weak inhibition to stimulation. Antioxidant and antiradical activities correlated strongly with total phenolic content, while emulsion stabilization did not correlate with lipase stimulation. Molecular modeling showed that cholate, escinescin Ia, osladin, and ginsenoside Rg1 did not persistently occlude the catalytic pocket, whereas several other saponins showed active-site blocking. Together, the results support a mechanism in which saponin-rich extracts regulate lipolysis through combined interfacial effects and compound-specific enzyme interactions.<\/jats:p>","DOI":"10.3390\/molecules31152600","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:49:27Z","timestamp":1785131367000},"page":"2600","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Interfacial and Molecular Mechanisms of Pancreatic Lipase Modulation by Saponin-Rich Extracts with Antioxidant Activity"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6618-017X","authenticated-orcid":false,"given":"Zbigniew","family":"Sroka","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, Borowska 211a, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8466-4098","authenticated-orcid":false,"given":"Karina","family":"Kapusta","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Physics and Atmospheric Sciences, Jackson State University, Jackson, MS 39217, USA"},{"name":"Department of Chemistry and Physics, Tougaloo College, Tougaloo, MS 39174, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9166-0527","authenticated-orcid":false,"given":"Senal D.","family":"Liyanage","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics, Tougaloo College, Tougaloo, MS 39174, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5169-7192","authenticated-orcid":false,"given":"Ta\u2019Miyia","family":"Tobias","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics, Tougaloo College, Tougaloo, MS 39174, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0276-7968","authenticated-orcid":false,"given":"Victoria","family":"Petrosyan","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics, Tougaloo College, Tougaloo, MS 39174, USA"},{"name":"St. Andrew\u2019s Episcopal School, Ridgeland, MS 39157, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7754-8969","authenticated-orcid":false,"given":"Wojciech","family":"Kolodziejczyk","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Physics and Atmospheric Sciences, Jackson State University, Jackson, MS 39217, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Imie\u0142owska","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Wroclaw Medical University, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Gle\u0144sk","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, Borowska 211a, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"\u017bbikowska","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, Borowska 211a, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2206-6591","authenticated-orcid":false,"given":"Andrzej","family":"Gamian","sequence":"additional","affiliation":[{"name":"Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7693-9469","authenticated-orcid":false,"given":"Kamil","family":"Wojciechowski","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Warmia and Mazury in Olsztyn, 10-721 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"142151","DOI":"10.1016\/j.foodchem.2024.142151","article-title":"Controlled Lipid Digestion in the Development of Functional and Personalized Foods for a Tailored Delivery of Dietary Fats","volume":"466","author":"Niu","year":"2025","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"102045","DOI":"10.1016\/j.cis.2019.102045","article-title":"Bile Salts in Digestion and Transport of Lipids","volume":"274","author":"Macierzanka","year":"2019","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_3","first-page":"57","article-title":"Chronic Pancreatitis","volume":"43","author":"Lucocq","year":"2025","journal-title":"Surgery"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"S44","DOI":"10.1002\/ppul.27013","article-title":"Pancreas and Pancreatitis: Exocrine Pancreatic Insufficiency","volume":"59","author":"Ramsey","year":"2024","journal-title":"Pediatr. Pulmonol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"goaf019","DOI":"10.1093\/gastro\/goaf019","article-title":"Etiologies of Exocrine Pancreatic Insufficiency","volume":"13","author":"Tang","year":"2025","journal-title":"Gastroenterol. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1023\/A:1018863227013","article-title":"Role of Gastrin\/CCK-B Receptor in the Regulation of Gastric Acid Secretion in Rat","volume":"42","author":"Tari","year":"1997","journal-title":"Dig. Dis. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1053\/j.gastro.2008.05.021","article-title":"Control of Gastric Acid Secretion in Health and Disease","volume":"134","author":"Schubert","year":"2008","journal-title":"Gastroenterology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"111804","DOI":"10.1016\/j.foodres.2022.111804","article-title":"Solubilization of Lipolysis Products in Mixed Micelles Is Enhanced in Presence of Bile Salts and Tween 80 as Revealed by a Model Study (Oleic Acid) and Emulsified Chia-Oil","volume":"161","author":"Naso","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"101785","DOI":"10.1016\/j.bpg.2022.101785","article-title":"Congenital Disorders of Intestinal Digestion and Absorption (Sugars, Proteins, Lipids, Ions)","volume":"56","author":"Peretti","year":"2022","journal-title":"Best Pract. Res. Clin. Gastroenterol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"80","DOI":"10.15407\/fz70.04.080","article-title":"Pathophysiological Features of Acute Liver Failure Caused by Cholestasis","volume":"70","author":"Kolosovych","year":"2024","journal-title":"Fiziol. Zh."},{"key":"ref_11","first-page":"6217","article-title":"Saponins as Natural Emulsifiers: Challenges, Regulatory Landscape, and Future in Biomedical and Cosmetic Fields","volume":"64","author":"Tom","year":"2025","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"102145","DOI":"10.1016\/j.cis.2020.102145","article-title":"Surface Activity and Foaming Properties of Saponin-Rich Plants Extracts","volume":"279","author":"Wojciechowski","year":"2020","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1078\/0065-1281-00534","article-title":"Hemolysis of Human Erythrocytes with Saponin Affects the Membrane Structure","volume":"102","author":"Baumann","year":"2000","journal-title":"Acta Histochem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"101642","DOI":"10.1016\/j.fochx.2024.101642","article-title":"Optimization of Saponin Extraction from the Leaves of Panax notoginseng and Panax quinquefolium and Evaluation of Their Antioxidant, Antihypertensive, Hypoglycemic and Anti-Inflammatory Activities","volume":"23","author":"Liang","year":"2024","journal-title":"Food Chem. X"},{"key":"ref_15","first-page":"221","article-title":"Significance and Efficacy of Triterpene Saponin Herbal Drugs with Expectorant Action in Cough Therapy","volume":"68","author":"Petrovic","year":"2022","journal-title":"Agric. For."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"20220337","DOI":"10.1515\/rams-2022-0337","article-title":"Nanoemulsions of Essential Oils Stabilized with Saponins Exhibiting Antibacterial and Antioxidative Properties","volume":"62","author":"Makiej","year":"2023","journal-title":"Rev. Adv. Mater. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Zong, J.-F., Hong, Z.-B., Hu, Z.-H., and Hou, R.-Y. (2025). Two New Triterpenoid Saponins with Antifungal Activity from Camellia sinensis Flowers. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26031147"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.foodchem.2018.07.145","article-title":"Inhibitory Effect of Quinoa and Fenugreek Extracts on Pancreatic Lipase and \u03b1-Amylase under in Vitro Traditional Conditions or Intestinal Simulated Conditions","volume":"270","author":"Herrera","year":"2019","journal-title":"Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1080\/09168451.2014.877830","article-title":"Effects of Two Sulfated Triterpene Saponins Echinoside A and Holothurin A on the Inhibition of Dietary Fat Absorption and Obesity Reduction","volume":"78","author":"Wang","year":"2014","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ejphar.2013.02.032","article-title":"Antiobesity Potential of Ursolic Acid Stearoyl Glucoside by Inhibiting Pancreatic Lipase","volume":"709","author":"Kazmi","year":"2013","journal-title":"Eur. J. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Rani, N., Vasudeva, N., and Sharma, S.K. (2012). Quality Assessment and Anti-Obesity Activity of Stellaria media (Linn.) Vill. BMC Complement. Altern. Med., 12.","DOI":"10.1186\/1472-6882-12-145"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1039\/C4FO00785A","article-title":"Lowering of Cholesterol Bioaccessibility and Serum Concentrations by Saponins: In Vitro and in Vivo Studies","volume":"6","author":"Vinarova","year":"2015","journal-title":"Food Funct."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1515\/znc-1997-3-415","article-title":"Stimulation of Pancreatic Lipase Activity by Saponins Isolated from Medicago sativa L.","volume":"52","author":"Sroka","year":"1997","journal-title":"Z. Naturforschung C"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Sroka, Z., Kapusta, K., Sucha\u0144ska, K., Ko\u0142odziejczyk, W., \u017bbikowska, B., Gamian, A., Strzelczyk, K., Gle\u0144sk, M., and Wojciechowski, K. (2025). Antiradical and Antioxidant Activity and Stimulation of Pancreatic Lipase by Extracts Obtained from Saponin-Rich Raw Materials: Experimental and In Silico Study. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms262110254"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zhang, C., Zuo, T., Wang, X., Wang, H., Hu, Y., Li, Z., Li, W., Jia, L., Qian, Y., and Yang, W. (2019). Integration of Data-Dependent Acquisition (DDA) and Data-Independent High-Definition MSE (HDMSE) for the Comprehensive Profiling and Characterization of Multicomponents from Panax japonicus by UHPLC\/IM-QTOF-MS. Molecules, 24.","DOI":"10.3390\/molecules24152708"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Yang, F., Chen, B., Jiang, M., Wang, H., Hu, Y., Wang, H., Xu, X., Gao, X., and Yang, W. (2022). Integrating Enhanced Profiling and Chemometrics to Unveil the Potential Markers for Differentiating among the Leaves of Panax ginseng, P. quinquefolius, and P. notoginseng by Ultra-High Performance Liquid Chromatography\/Ion Mobility-Quadrupole Time-of-Flight Mass Spectrometry. Molecules, 27.","DOI":"10.3390\/molecules27175549"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s13361-018-2089-5","article-title":"Various Multicharged Anions of Ginsenosides in Negative Electrospray Ionization with QTOF High-Resolution Mass Spectrometry","volume":"30","author":"Zhao","year":"2019","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"18007","DOI":"10.1074\/jbc.271.30.18007","article-title":"Lipase Activation by Nonionic Detergents","volume":"271","author":"Hermoso","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref_29","first-page":"1","article-title":"A Brief Review of Phenolic Compounds Identified from Plants: Their Extraction, Analysis, and Biological Activity","volume":"17","author":"Zhang","year":"2022","journal-title":"Nat. Prod. Commun."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"82","DOI":"10.3923\/ijp.2014.82.89","article-title":"The Mechanism Underlying the Spasmolytic and Bronchodilatory Activities of the Flavonoid-Rich Red Onion \u201cAllium cepa L.\u201d Peel Extract","volume":"10","author":"Mandukhail","year":"2014","journal-title":"Int. J. Pharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"29004","DOI":"10.1038\/s41598-025-13832-4","article-title":"Accelerated Wound Healing through Tannin-Rich Jaft Extract, Concentration-Dependent Efficacy and Mechanistic Insights from Quercus brantii Ointment Formulations","volume":"15","author":"Samavati","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"03000605231167314","DOI":"10.1177\/03000605231167314","article-title":"Proanthocyanidins May Be Potential Therapeutic Agents for the Treatment of Carotid Atherosclerosis: A Review","volume":"51","author":"Huang","year":"2023","journal-title":"J. Int. Med. Res."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Ma\u0144kowska, D., and Dems-Rudnicka, K. (2025). Antioxidant Activity and Phenolic Compounds in Medicinal Plants: A Comparison of Organic and Conventional Mentha piperita, Melissa officinalis, Salvia officinalis, and Urtica dioica. Molecules, 30.","DOI":"10.3390\/molecules30244812"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.biopha.2016.11.112","article-title":"Adaptogenic Herb Ginseng (Panax) as Medical Food: Status Quo and Future Prospects","volume":"85","author":"Patel","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"108827","DOI":"10.1016\/j.pharmthera.2025.108827","article-title":"The Anti-Inflammatory Effects of Saponins from Natural Herbs","volume":"269","author":"Zheng","year":"2025","journal-title":"Pharmacol. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1055\/s-2008-1068628","article-title":"Wirkung von Ro\u00dfkastaniensamenextrakt Auf Die Transkapill\u00e4re Filtration Bei Chronischer Ven\u00f6ser Insuffizienz","volume":"111","author":"Bisler","year":"2008","journal-title":"DMW Dtsch. Med. Wochenschr."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Bustos, K.A.G., Mu\u00f1oz, S.S., da Silva, S.S., Alarcon, M.A.D.F., dos Santos, J.C., Andrade, G.J.C., and Hilares, R.T. (2024). Saponin Molecules from Quinoa Residues: Exploring Their Surfactant, Emulsifying, and Detergent Properties. Molecules, 29.","DOI":"10.3390\/molecules29204928"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.foodhyd.2016.03.018","article-title":"On the Role of Bile Salts in the Digestion of Emulsified Lipids","volume":"60","author":"Sarkar","year":"2016","journal-title":"Food Hydrocoll."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"118363","DOI":"10.1016\/j.ejmech.2025.118363","article-title":"Recent Advances in Saponin-Lipase-Microbiota Triad Effect on Obesity Management, a Comprehensive Review","volume":"302","author":"Abdelfattah","year":"2026","journal-title":"Eur. J. Med. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0944-7113(99)80018-X","article-title":"Lipoprotein Lipase Activation by Red Ginseng Saponins in Hyperlipidemia Model Animals","volume":"6","author":"Inoue","year":"1999","journal-title":"Phytomedicine"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"290","DOI":"10.3181\/00379727-36-9204P","article-title":"Effects of Saponin and Digitonin on Lipase and Phosphatase Action","volume":"36","author":"Gould","year":"1937","journal-title":"Exp. Biol. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.colsurfa.2010.11.027","article-title":"Dynamic Interfacial Tensions of Dietary Oils","volume":"382","author":"Dopierala","year":"2011","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1007\/s11743-017-2016-y","article-title":"Surface Tension and Adsorption Studies by Drop Profile Analysis Tensiometry","volume":"20","author":"Kairaliyeva","year":"2017","journal-title":"J. Surfactants Deterg."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3537","DOI":"10.1021\/acs.langmuir.2c03511","article-title":"Surfactant Adsorption Layers: Experiments and Modeling","volume":"39","author":"Kovalchuk","year":"2023","journal-title":"Langmuir"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.colsurfa.2015.12.001","article-title":"Surface Properties of Adsorption Layers Formed from Triterpenoid and Steroid Saponins","volume":"491","author":"Pagureva","year":"2016","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"5738","DOI":"10.1039\/c3sm27950b","article-title":"Remarkably High Surface Visco-Elasticity of Adsorption Layers of Triterpenoid Saponins","volume":"9","author":"Golemanov","year":"2013","journal-title":"Soft Matter"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"100400","DOI":"10.1016\/j.colcom.2021.100400","article-title":"Surface Activity of the Oat, Horse Chestnut, Cowherb, Soybean, Quinoa and Soapwort Extracts\u2014Is It Only Due to Saponins?","volume":"42","author":"Stochmal","year":"2021","journal-title":"Colloid Interface Sci. Commun."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1002\/jsfa.2740340212","article-title":"Saponin Content of Food Plants and Some Prepared Foods","volume":"34","author":"Fenwick","year":"1983","journal-title":"J. Sci. Food Agric."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"11226","DOI":"10.1021\/jf901707z","article-title":"Quantification of Soyasaponins I and \u0392g in Italian Lentil Seeds by Solid-Phase Extraction (SPE) and High-Performance Liquid Chromatography\u2212Mass Spectrometry (HPLC-MS)","volume":"57","author":"Sagratini","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1292\/jvms.67.235","article-title":"Mild Hepatic Fibrosis in Cholesterol and Sodium Cholate Diet-Fed Rats","volume":"67","author":"Jeong","year":"2005","journal-title":"J. Vet. Med. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Ichimura-Shimizu, M., Watanabe, S., Kashirajima, Y., Nagatomo, A., Wada, H., Tsuneyama, K., and Omagari, K. (2022). Dietary Cholic Acid Exacerbates Liver Fibrosis in NASH Model of Sprague\u2013Dawley Rats Fed a High-Fat and High-Cholesterol Diet. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23169268"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3521","DOI":"10.1007\/s11033-020-05442-2","article-title":"Concentration-Dependent Effects of Sodium Cholate and Deoxycholate Bile Salts on Breast Cancer Cells Proliferation and Survival","volume":"47","author":"Fontana","year":"2020","journal-title":"Mol. Biol. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.ijpharm.2018.08.031","article-title":"Bile Salt Enhancers for Inhalation: Correlation between in Vitro and in Vivo Lung Effects","volume":"550","author":"Hougaard","year":"2018","journal-title":"Int. J. Pharm."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"100897","DOI":"10.1016\/j.jgr.2025.08.008","article-title":"The Role of Ginseng and Ginsenosides in ROS and Cardiovascular Disease","volume":"50","author":"Vijayakumar","year":"2026","journal-title":"J. Ginseng Res."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Xu, Z.-M., Li, C.-B., Liu, Q.-L., Li, P., and Yang, H. (2018). Ginsenoside Rg1 Prevents Doxorubicin-Induced Cardiotoxicity through the Inhibition of Autophagy and Endoplasmic Reticulum Stress in Mice. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19113658"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1007\/s11626-025-01070-x","article-title":"Ginsenoside Rg1 Mitigates Myocardial Ischemia\/Reperfusion Injury by Inhibiting NLRP3-Mediated Pyroptosis","volume":"61","author":"Li","year":"2025","journal-title":"In Vitro Cell. Dev. Biol. Anim."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"106805","DOI":"10.1016\/j.intimp.2020.106805","article-title":"Ginsenoside Rg1 Prevent and Treat Inflammatory Diseases: A Review","volume":"87","author":"Gao","year":"2020","journal-title":"Int. Immunopharmacol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.envpol.2018.01.058","article-title":"What Is the Aquatic Toxicity of Saponin-Rich Plant Extracts Used as Biopesticides?","volume":"236","author":"Jiang","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/0009-8981(66)90215-4","article-title":"A Specific Method for Serum Lipase Determination","volume":"13","author":"Tietz","year":"1966","journal-title":"Clin. Chim. Acta"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"105003","DOI":"10.1016\/j.arabjc.2023.105003","article-title":"Micro-Titer Plate Assay for Measurement of Total Phenolic and Total Flavonoid Contents in Medicinal Plant Extracts","volume":"16","author":"Sari","year":"2023","journal-title":"Arab. J. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.foodchem.2016.12.008","article-title":"Combined Microplate-ABTS and HPLC-ABTS Analysis of Tomato and Pepper Extracts Reveals Synergetic and Antagonist Effects of Their Lipophilic Antioxidative Components","volume":"223","author":"Guffond","year":"2017","journal-title":"Food Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3470","DOI":"10.1021\/jf703723s","article-title":"High-Throughput Methods To Assess Lipophilic and Hydrophilic Antioxidant Capacity of Food Extracts in Vitro","volume":"56","author":"Giuffrida","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S0927-7757(96)03857-5","article-title":"Determination of Equilibrium Surface Tension Values by Extrapolation via Long Time Approximations","volume":"122","author":"Makievski","year":"1997","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Loglio, G., Pandolfini, P., Miller, R., Makievski, A.V., Ravera, F., and Liggieri, L. (2001). Drop and Bubble Shape Analysis as a Tool For Dilational Rheological Studies of Interfacial Layers. Studies in Interface Science, Elsevier.","DOI":"10.1016\/S1383-7303(01)80038-7"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"4291","DOI":"10.1021\/acs.jctc.1c00302","article-title":"OPLS4: Improving Force Field Accuracy on Challenging Regimes of Chemical Space","volume":"17","author":"Lu","year":"2021","journal-title":"J. Chem. Theory Comput."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2380","DOI":"10.1021\/acs.jctc.3c00044","article-title":"Epik: P K a and Protonation State Prediction through Machine Learning","volume":"19","author":"Johnston","year":"2023","journal-title":"J. Chem. Theory Comput."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"6177","DOI":"10.1021\/jm051256o","article-title":"Extra Precision Glide: Docking and Scoring Incorporating a Model of Hydrophobic Enclosure for Protein\u2212Ligand Complexes","volume":"49","author":"Friesner","year":"2006","journal-title":"J. Med. Chem."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1021\/ci300425v","article-title":"MM\/GBSA Binding Energy Prediction on the PDBbind Data Set: Successes, Failures, and Directions for Further Improvement","volume":"53","author":"Greenidge","year":"2013","journal-title":"J. Chem. Inf. Model."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Bowers, K.J., Chow, D.E., Xu, H., Dror, R.O., Eastwood, M.P., Gregersen, B.A., Klepeis, J.L., Kolossvary, I., Moraes, M.A., and Sacerdoti, F.D. (2006). Scalable Algorithms for Molecular Dynamics Simulations on Commodity Clusters. Proceedings of the SC\u201906: 2006 ACM\/IEEE Conference on Supercomputing, IEEE.","DOI":"10.1145\/1188455.1188544"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"W513","DOI":"10.1093\/nar\/gkae303","article-title":"ProTox 3.0: A Webserver for the Prediction of Toxicity of Chemicals","volume":"52","author":"Banerjee","year":"2024","journal-title":"Nucleic Acids Res."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/15\/2600\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T04:15:45Z","timestamp":1785212145000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/15\/2600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,25]]},"references-count":70,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["molecules31152600"],"URL":"https:\/\/doi.org\/10.3390\/molecules31152600","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,25]]}}}