{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:05:13Z","timestamp":1785240313088,"version":"3.55.0"},"reference-count":65,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Helichrysum arenarium (L.) Moench is a European native species typically associated with dry, sunny, sandy, and nutrient-poor habitats. In European herbal practice, preparations from H. arenarium inflorescences are traditionally used for the symptomatic relief of digestive complaints. Polyphenolic constituents, particularly flavonoid and chalcone derivatives, are among the most characteristic compounds detected in the inflorescences. Intensive harvesting and degradation of natural habitats have increased pressure on wild populations. Since H. arenarium is legally protected in many countries, biotechnological approaches for plant biomass production represent a promising alternative. In the present study, shoot and callus cultures were established, and detailed extract profiling was performed using ultra-high-performance liquid chromatography coupled with diode-array detection and electrospray ionization multistage mass spectrometry (UHPLC-DAD-ESI-MS3). Cytotoxic, antioxidant, anti-inflammatory, wound-healing, anti-collagenase, and anti-hyaluronidase activities were also evaluated. In vitro-derived materials were compared with inflorescences and shoots of the maternal plant. The analyzed materials showed pronounced tissue-dependent metabolic differentiation. The highest callus growth rate was observed for the Ha-C-1 line, which also accumulated the highest levels of chlorogenic acid (25.80 \u00b1 3.09 mg\/g dry extract) and isochlorogenic acid A (55.83 \u00b1 13.78 mg\/g dry extract). Apigenin was detected in the inflorescence extract, reaching 3.55 \u00b1 1.42 mg\/g dry extract. The extracts did not markedly affect HaCaT viability at 15.63\u2013500 \u00b5g\/mL, while the strongest bioactivities were observed for inflorescence and maternal plant extracts.<\/jats:p>","DOI":"10.3390\/molecules31152625","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T11:45:26Z","timestamp":1785239126000},"page":"2625","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antioxidant and Anti-Aging Activity of Helichrysum arenarium (L.) Moench: Comparative Evaluation of Extracts from Shoots, Inflorescences, Plantlets and Callus Tissue"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6524-8046","authenticated-orcid":false,"given":"Joanna","family":"Jab\u0142o\u0144ska","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9029-5755","authenticated-orcid":false,"given":"Maciej","family":"Obr\u0119bski","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, Poland"},{"name":"MicrobiotaLab, Department of Pharmaceutical Microbiology and Bioanalysis, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0058-6433","authenticated-orcid":false,"given":"Rafa\u0142 M.","family":"Kie\u0142kiewicz","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irena","family":"Macias","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Bogusz","sequence":"additional","affiliation":[{"name":"Faculty of Biochemistry, Biophysics and Biotechnology at the Jagiellonian University, 7 Gronostajowa St., 30-387 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6960-2152","authenticated-orcid":false,"given":"Weronika","family":"Skowro\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9150-7872","authenticated-orcid":false,"given":"Katarzyna","family":"Syk\u0142owska-Baranek","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,28]]},"reference":[{"key":"ref_1","unstructured":"(2026, May 18). 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