{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:11:14Z","timestamp":1785431474218,"version":"3.56.0"},"reference-count":53,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22073039"],"award-info":[{"award-number":["22073039"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["22073039"],"award-info":[{"award-number":["22073039"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Major Longitudinal Project of Liaocheng University for Cultivation","award":["318062303"],"award-info":[{"award-number":["318062303"]}]},{"award":["318062303"],"award-info":[{"award-number":["318062303"]}],"id":[{"id":"https:\/\/ror.org\/03yh0n709","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Doctoral Starting up Foundation of Liaocheng University","award":["318052262"],"award-info":[{"award-number":["318052262"]}]},{"award":["318052262"],"award-info":[{"award-number":["318052262"]}],"id":[{"id":"https:\/\/ror.org\/03yh0n709","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Advanced glycation end products (AGEs) are implicated in the pathogenesis of chronic diseases. This study investigated the inhibitory effect of theaflavin (TA) on AGE formation in a human serum albumin (HSA)-fructose model system, both in the absence and presence of non-cytotoxic Mg2+ or Mn2+. The underlying mechanism was elucidated using multiple spectroscopic techniques and molecular docking. TA significantly inhibited AGE formation, with the enhancement by metal ions following the order: 1.5 \u03bcM Mn2+ &gt; 1.5 \u03bcM Mg2+ &gt; 1 mM Mg2+. This trend aligned with the binding affinity between HSA and TA derived from molecular interaction studies. Site marker competition and docking results revealed that TA binds preferentially within subdomain IIA of HSA. Furthermore, the conformational changes in HSA following glycation and inhibition were monitored, along with the influence of metal ions on the antioxidant activity of TA. By integrating the results, it was concluded that free radical scavenging contributes more significantly to AGE inhibition than does blocking the glycation sites on HSA. Overall, this study elucidates the influence of metal ions on the inhibitory effect of TA against AGE formation and the corresponding mechanism, providing insights for the prevention and management of chronic diseases.<\/jats:p>","DOI":"10.3390\/molecules31152648","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T17:01:46Z","timestamp":1785344506000},"page":"2648","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unveiling the Role of Mg2+ and Mn2+ in Theaflavin-Mediated Inhibition of Advanced Glycation End Products Formation: Mechanistic Insights from Intermolecular Interaction"],"prefix":"10.3390","volume":"31","author":[{"given":"Lixia","family":"Yuan","sequence":"first","affiliation":[{"name":"Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, Institute of BioPharmaceutical Research, Liaocheng University, Liaocheng 252059, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Liu","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, Institute of BioPharmaceutical Research, Liaocheng University, Liaocheng 252059, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Lu","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, Institute of BioPharmaceutical Research, Liaocheng University, Liaocheng 252059, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhining","family":"Yan","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, Institute of BioPharmaceutical Research, Liaocheng University, Liaocheng 252059, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1918-9072","authenticated-orcid":false,"given":"Min","family":"Liu","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, Institute of BioPharmaceutical Research, Liaocheng University, Liaocheng 252059, China"},{"name":"School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"ref_1","unstructured":"(2024, May 21). World Health Statistics 2024: Monitoring Health for the SDGs, Sustainable Development Goals. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789240094703."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Kataoka, H. (2025). Current developments in analytical methods for advanced glycation end products in foods. Molecules, 30.","DOI":"10.3390\/molecules30204095"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"137401","DOI":"10.1016\/j.jcis.2025.137401","article-title":"Development of an aminoguanidine hybrid hydrogel composites with hydrogen and oxygen supplying performance to boost infected diabetic wound healing","volume":"691","author":"Yang","year":"2025","journal-title":"J. Colloid. Interf. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Wei, X., Ma, Y.B., Li, Y., Zhang, W.Z., Zhong, Y.T., Yu, Y., Zhang, L.C., Wang, Z.B., and Tu, Y. (2022). Anti-apoptosis of podocytes and pro-apoptosis of mesangial cells for telmisartan in alleviating diabetic kidney injury. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.876469"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1007\/s40618-020-01231-7","article-title":"Serum levels of advanced glycation end products (AGEs) are increased and their soluble receptor (sRAGE) reduced in Hashimoto\u2019s thyroiditis","volume":"43","author":"Ruggeri","year":"2020","journal-title":"J. Endocrinol. Investig."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"143178","DOI":"10.1016\/j.foodchem.2025.143178","article-title":"Mechanistic insights into advanced glycation end products production in glucose-lysine model system involved with ascorbic acid","volume":"474","author":"Song","year":"2025","journal-title":"Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"145378","DOI":"10.1016\/j.ijbiomac.2025.145378","article-title":"Effects of flavonol B-ring structures on the inhibitions of advanced glycation end products in bovine serum albumin-methylglyoxal models","volume":"319","author":"Ling","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4621","DOI":"10.1021\/acs.jafc.8b00650","article-title":"Novel flavoalkaloids from white tea with inhibitory activity against the formation of advanced glycation end products","volume":"66","author":"Li","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4102","DOI":"10.1021\/jf300347p","article-title":"Inhibition of advanced glycation end product formation by Pu-erh tea ameliorates progression of experimental diabetic nephropathy","volume":"60","author":"Yan","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"139285","DOI":"10.1016\/j.foodchem.2024.139285","article-title":"Research progress on the functions and biosynthesis of theaflavins","volume":"450","author":"Liu","year":"2024","journal-title":"Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1007\/s12640-012-9332-9","article-title":"Therapeutic attenuation of neuroinflammation and apoptosis by black tea theaflavin in chronic MPTP\/probenecid model of Parkinson\u2019s disease","volume":"23","author":"Anandhan","year":"2013","journal-title":"Neurotox. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"151","DOI":"10.31665\/JFB.2018.3158","article-title":"Anti-diabetic effects of a theaflavin-enriched black tea extract in the obese ZDF rat model","volume":"3","author":"Gosslaua","year":"2018","journal-title":"J. Food Bioact."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1080\/09168451.2017.1282805","article-title":"Quercetin inhibits advanced glycation end product formation via chelating metal ions, trapping methylglyoxal, and trapping reactive oxygen species","volume":"81","author":"Bhuiyan","year":"2017","journal-title":"Biosci. Biotech. Biochem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"100676","DOI":"10.1016\/j.blre.2020.100676","article-title":"Magnesium: The overlooked electrolyte in blood cancers","volume":"44","author":"Gile","year":"2020","journal-title":"Blood Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"16077","DOI":"10.1039\/D5CP01244A","article-title":"Effect of magnesium(II) on the glycation-induced oxidative stress of human serum albumin: A spectroscopic investigation","volume":"27","author":"Brahma","year":"2025","journal-title":"Phys. Chem. Chem. Phys."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"115941","DOI":"10.1016\/j.jpba.2023.115941","article-title":"An immobilization of 2-(Aminomethyl) thiazole on multi-walled carbon nanotubes used for rapid extraction of manganese ions in hepatic patients","volume":"240","author":"Zarandi","year":"2024","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"131\u2212139","DOI":"10.1016\/j.lfs.2011.10.025","article-title":"Effects of zinc and manganese on advanced glycation end products (AGEs) formation and AGEs-mediated endothelial cell dysfunction","volume":"90","author":"Zhuang","year":"2012","journal-title":"Life Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"111750","DOI":"10.1016\/j.biopha.2021.111750","article-title":"Novel advances in inhibiting advanced glycation end product formation using natural compounds","volume":"140","author":"Song","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Alrehaili, J., and Anwer, R. (2026). Inhibition of glucose-induced glycation of HSA by pioglitazone: Multi-spectroscopic and bioinformatic evidence. Molecules, 31.","DOI":"10.3390\/molecules31091519"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/s43556-025-00287-2","article-title":"Fructose metabolism and its roles in metabolic diseases, inflammatory diseases, and cancer","volume":"6","author":"Li","year":"2025","journal-title":"Mol. Biomed."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"143278","DOI":"10.1016\/j.ijbiomac.2025.143278","article-title":"Probing the effect of glycation on the pseudo-esterase activity of Human Serum Albumin","volume":"310","author":"Chaki","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"6906","DOI":"10.1021\/jf201103t","article-title":"Modified fast procedure for the detection and screening of antiglycative phytochemicals","volume":"59","author":"Wang","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1002\/jcla.20048","article-title":"Rapid method for the preparation of an AGE-BSA standard calibrator using thermal glycation","volume":"19","author":"Bhatwadekar","year":"2005","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"126322","DOI":"10.1016\/j.molliq.2024.126322","article-title":"Inhibitory effect of caffeic acid and dihydrocaffeic acid on the formation of advanced glycation end products: Mechanism analysis based on intermolecular interaction","volume":"415","author":"Zhang","year":"2024","journal-title":"J. Mol. Liq."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.ijbiomac.2020.06.048","article-title":"Rosmarinic acid restrains protein glycation and aggregation in human serum albumin: Multi spectroscopic and microscopic insight\u2014Possible Therapeutics Targeting Diseases","volume":"161","author":"Shamsi","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"133295","DOI":"10.1016\/j.foodchem.2022.133295","article-title":"Inhibitory effects of polyphenols from black chokeberry on advanced glycation end-products (AGEs) formation","volume":"392","author":"Zhao","year":"2022","journal-title":"Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1515\/reveh-2019-0008","article-title":"An overview on role of some trace elements in human reproductive health, sperm function and fertilization process","volume":"34","author":"Mirnamniha","year":"2019","journal-title":"Rev. Environ. Health"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"9250107","DOI":"10.26599\/FSHW.2024.9250107","article-title":"Effect of ellagitannin metabolites ellagic acid and urolithins A-D on the non-enzymatic glycation of human serum albumin and inhibiting mechanisms","volume":"14","author":"Peng","year":"2025","journal-title":"Food Sci. Hum. Well."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"101313","DOI":"10.1016\/j.fbio.2021.101313","article-title":"Insights into oat polyphenols constituent against advanced glycation end products mechanism by spectroscopy and molecular interaction","volume":"43","author":"Qin","year":"2021","journal-title":"Food Biosci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"102857","DOI":"10.1016\/j.fochx.2025.102857","article-title":"Study on the inhibitory effects and mechanisms of four polyphenolic compounds from perilla extract on advanced glycation end products (AGEs)","volume":"29","author":"Han","year":"2025","journal-title":"Food Chem. X"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1080\/07391102.2023.2201856","article-title":"Inhibitory effect of baicalein against glycation in HSA: An in vitro approach","volume":"42","author":"Riaz","year":"2024","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/j.ijbiomac.2019.06.102","article-title":"Effect of divalent cations on bovine serum albumin (BSA) and tannic acid interaction and its influence on turbidity and in vitro protein digestibility","volume":"136","author":"Kaspchak","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"108022","DOI":"10.1016\/j.foodhyd.2022.108022","article-title":"pH-dependent interaction mechanisms between \u03b2-lactoglobulin and EGCG: Insights from multi-spectroscopy and molecular dynamics simulation methods","volume":"133","author":"Liu","year":"2022","journal-title":"Food Hydrocoll."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"135805","DOI":"10.1016\/j.molstruc.2023.135805","article-title":"Binding effects of piceatannol and oxyresveratrol on the structural and functional properties of soy protein isolate","volume":"1288","author":"Yuan","year":"2023","journal-title":"J. Mol. Struct."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"111398","DOI":"10.1016\/j.foodhyd.2025.111398","article-title":"Enhancing protein interactions in Zein-\u03b2-lactoglobulin complexes by (-)-Epigallocatechin-3-gallate: Expanding the potential of molecular glues in food application","volume":"167","author":"Jiao","year":"2025","journal-title":"Food Hydrocoll."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Xu, J.H., Wang, M.Y., Zheng, Y.Z., and Tang, L. (2019). Spectroscopic technique-based comparative investigation on the interaction of theaflavins with native and glycated human serum albumin. Molecules, 24.","DOI":"10.3390\/molecules24173171"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.saa.2018.08.028","article-title":"Mutual influence of piceatannol and bisphenol F on their interaction with pepsin: Insights from spectroscopic, isothermal titration calorimetry and molecular modeling studies","volume":"206","author":"Shi","year":"2019","journal-title":"Spectrochim. Acta Part A"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"106465","DOI":"10.1016\/j.foodhyd.2020.106465","article-title":"Hydrophobic interaction driving the binding of soybean protein isolate and chlorophyll: Improvements to the thermal stability of chlorophyll","volume":"113","author":"Cao","year":"2021","journal-title":"Food Hydrocoll."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2549","DOI":"10.1021\/acs.chemrestox.1c00311","article-title":"Extending the HSA-Cys34-adductomics pipeline to modifications at Lys525","volume":"34","author":"Grigoryan","year":"2021","journal-title":"Chem. Res. Toxicol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/j.saa.2016.10.023","article-title":"Competitive binding of (\u2212)-epigallocatechin-3-gallate and 5-fluorouracil to human serum albumin: A fluorescence and circular dichroism study","volume":"173","author":"Yuan","year":"2017","journal-title":"Spectrochim. Acta Part A"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.ijbiomac.2020.10.174","article-title":"Inhibitory mechanism of sinensetin on \u03b1-glucosidase and non-enzymatic glycation: Insights from spectroscopy and molecular docking analyses","volume":"166","author":"Liu","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Zhang, X.Y., Jiang, Q.N., Liu, J.J., Wang, H., Lu, H.Y., Liu, D.T., Wen, P.W., Tu, Z.C., and Hu, Y.M. (2025). Effect of different high-temperature heating methods on the glycation reaction and advanced glycation reaction products of \u03b2-lactoglobulin. Foods, 14.","DOI":"10.3390\/foods14213722"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"131385","DOI":"10.1016\/j.foodchem.2021.131385","article-title":"Antioxidant activity, stability, in vitro digestion and cytotoxicity of two dietary polyphenols co-loaded by \u03b2-lactoglobulin","volume":"371","author":"Ren","year":"2022","journal-title":"Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"8896","DOI":"10.1021\/jf0715166","article-title":"Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements","volume":"55","author":"Wolfe","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"496\u2212500","DOI":"10.5694\/j.1326-5377.1968.tb82970.x","article-title":"Interrelationship of magnesium and ionized calcium in human magnesium deficiency","volume":"2","author":"Zimmet","year":"1968","journal-title":"Med. J. Aust."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1826","DOI":"10.1038\/sj.cdd.4402187","article-title":"p38 MAPK and MSK1 mediate caspase-8 activation in manganese-induced mitochondria-dependent cell death","volume":"14","author":"Mchichi","year":"2007","journal-title":"Cell Death Differ."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"9505","DOI":"10.1073\/pnas.181319898","article-title":"Mitochondria play no roles in Mn(II)-induced apoptosis in HeLa cells","volume":"98","author":"Oubrahim","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1016\/j.ijbiomac.2019.11.080","article-title":"Mitigation of isoquercitrin on \u03b2-lactoglobulin glycation: Insight into the mechanisms by mass spectrometry and interaction analysis","volume":"155","author":"Ma","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Wei, Y.S., Gong, D.M., and Zhang, G.W. (2026). Mechanisms of isoliquiritigenin against protein glycation: A comparative study in PBS solution and crowding environment. Foods, 15.","DOI":"10.3390\/foods15101796"},{"key":"ref_50","first-page":"e-20250181","article-title":"Multi-spectroscopic and molecular docking insights into the effects of structural differences among three curcuminoids on \u03b2-lactoglobulin glycation","volume":"37","author":"Zhang","year":"2026","journal-title":"J. Braz. Chem. Soc."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Molkova, E.A., Sarimov, R.M., Matveeva, T.A., Simakin, A.V., Akopdzhanov, A.G., Sharafullin, P., Pichkur, P., Dorokhov, A.S., Izmaylov, A.Y., and Gudkov, S.V. (2026). Comparison of protein binding and fluorescence quenching by gold and iron oxide nanoparticles: pH and temperature dependence. Molecules, 31.","DOI":"10.3390\/molecules31122008"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"124090","DOI":"10.1016\/j.saa.2024.124090","article-title":"Multi-spectroscopic and molecular docking studies for the pH-dependent interaction of \u03b2-lactoglobulin with (\u2212)-epicatechin gallate and\/or piceatannol: Influence on antioxidant activity and stability","volume":"313","author":"Yuan","year":"2024","journal-title":"Spectrochim. Acta Part A"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"120986","DOI":"10.1016\/j.saa.2022.120986","article-title":"Multispectroscopic and synergistic antioxidant study on the combined binding of caffeic acid and (\u2212)-epicatechin gallate to lysozyme","volume":"272","author":"Liu","year":"2022","journal-title":"Spectrochim. Acta Part A"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/15\/2648\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:32:22Z","timestamp":1785403942000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/15\/2648"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,29]]},"references-count":53,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["molecules31152648"],"URL":"https:\/\/doi.org\/10.3390\/molecules31152648","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,29]]}}}