{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T04:42:10Z","timestamp":1786250530059,"version":"build-2736575974"},"reference-count":54,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Department of Science and Technology-Science and Engineering Research Board (DST-SERB) of India","doi-asserted-by":"publisher","award":["CRG\/2023\/001796"],"award-info":[{"award-number":["CRG\/2023\/001796"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"award":["CRG\/2023\/001796"],"award-info":[{"award-number":["CRG\/2023\/001796"]}],"id":[{"id":"https:\/\/ror.org\/03ffdsr55","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council of Taiwan","doi-asserted-by":"crossref","award":["NSTC 112-2320-B-038-037-MY3"],"award-info":[{"award-number":["NSTC 112-2320-B-038-037-MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council of Taiwan","doi-asserted-by":"crossref","award":["NSTC 114-2320-B-038-033-MY3"],"award-info":[{"award-number":["NSTC 114-2320-B-038-033-MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University Grant Commission (UGC) of India under the JRF fellowship scheme","award":["220510291550"],"award-info":[{"award-number":["220510291550"]}]},{"award":["220510291550"],"award-info":[{"award-number":["220510291550"]}],"id":[{"id":"https:\/\/ror.org\/04p800546","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Natural products represent an important source of bioactive compounds with therapeutic potential in the fields of thrombotic and oxidative stress-related disorders. This study focuses on identifying active compounds from the ethanolic extract of Ocimum sanctum L. leaves (EEOSL) and validating its antiplatelet and hepatoprotective activities. GC-MS and LC-MS (both positive and negative ionization modes) analyses identified 155 and 113 compounds, respectively, constituting several bioactive compounds. Collagen-induced human platelet activation (CIHPA) and carbon tetrachloride (CCl4)-induced hepatotoxicity were used as rat models to assess the activity of EEOSL. EEOSL (1\u201310 mg\/mL) was shown to significantly and dose-dependently inhibit collagen-induced platelet aggregation. The extract also inhibited P-selectin expression, ATP release and intracellular Ca2+ mobilization, suggesting inhibition of major pathways involved in platelet activation. To understand the mechanisms, molecular docking was carried out with key platelet signaling molecules such as GPVI, SYK, PLC\u03b32, P2RY12, and PI3K\u03b2. Some phytoconstituents showed good binding affinities, with apigenin having the highest binding affinity for PI3K\u03b2 (\u22128.01 kcal\/mol). This binding was further validated by molecular dynamics simulations showing the formation of stable complexes. Intraperitoneal injection of CCl4 significantly increased the serum hepatic markers (SGOT, SGPT, LDH and SALP); EEOSL treatment significantly reduced these increases in vivo. The extract normalized antioxidant enzyme activities (catalase, SOD and glutathione peroxidase) and antioxidant isozyme patterns. Histopathological results revealed a significant level of protection against liver damage. Overall, the results showed that EEOSL has phytoconstituents that could provide strong antiplatelet and hepatoprotective properties, likely due to their ability to modulate platelet signaling pathways and enhance antioxidant defense mechanisms.<\/jats:p>","DOI":"10.3390\/molecules31162749","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T11:44:53Z","timestamp":1786103093000},"page":"2749","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective Effects of the Ethanolic Leaf Extract of Ocimum sanctum L. on Collagen-Induced Human Platelet Aggregation and CCl4-Induced Hepatotoxicity in Rats: Molecular Docking Analysis of Platelet Activation Signaling Targets"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4548-9994","authenticated-orcid":false,"given":"Mohammad","family":"Maaz","sequence":"first","affiliation":[{"name":"Environmental Toxicology and Molecular Phytotherapy Lab, Department of Ecology and Environmental Sciences, School of Life Sciences, Pondicherry University, Puducherry 605014, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agilan","family":"Balupillai","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry 605014, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2056-4607","authenticated-orcid":false,"given":"Saravanabhavan","family":"Periyakali","sequence":"additional","affiliation":[{"name":"Department of Zoology, Bharathiar University, Coimbatore 641046, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3992-5616","authenticated-orcid":false,"given":"Jasmin","family":"Padhan","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry 605014, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumar","family":"Kalavathy Murugan","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry 605014, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8265-3678","authenticated-orcid":false,"given":"Joen-Rong","family":"Sheu","sequence":"additional","affiliation":[{"name":"Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayakumar","family":"Thanasekaran","sequence":"additional","affiliation":[{"name":"Environmental Toxicology and Molecular Phytotherapy Lab, Department of Ecology and Environmental Sciences, School of Life Sciences, Pondicherry University, Puducherry 605014, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3415","DOI":"10.1242\/jcs.01325","article-title":"Platelet adhesion signalling and the regulation of thrombus formation","volume":"117","author":"Gibbins","year":"2004","journal-title":"J. Cell Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1111\/j.1538-7836.2009.03455.x","article-title":"Calcium signaling in platelets","volume":"7","author":"Braun","year":"2009","journal-title":"J. Thromb. Haemost."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2341","DOI":"10.1161\/ATVBAHA.110.207522","article-title":"Signaling during platelet adhesion and activation","volume":"30","author":"Li","year":"2010","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.blre.2014.10.003","article-title":"Platelet secretion: From haemostasis to wound healing and beyond","volume":"29","author":"Golebiewska","year":"2015","journal-title":"Blood Rev."},{"key":"ref_5","unstructured":"Michelson, A.D., Cattaneo, M., Frelinger, A.L., and Newman, P.J. (2019). Platelets, Academic Press. [4th ed.]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1161\/hc0902.104353","article-title":"Inflammation and atherosclerosis","volume":"105","author":"Libby","year":"2002","journal-title":"Circulation"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2373","DOI":"10.1056\/NEJMra052717","article-title":"Low-dose aspirin for the prevention of atherothrombosis","volume":"353","author":"Patrono","year":"2005","journal-title":"N. Engl. J. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2757","DOI":"10.1182\/blood-2005-03-1047","article-title":"Platelet NAD(P)H oxidase-generated ROS production regulates \u03b1IIb\u03b23-integrin activation independent of the NO\/cGMP pathway","volume":"106","author":"Begonja","year":"2005","journal-title":"Blood"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/B978-0-12-405881-1.00002-1","article-title":"Reactive oxygen species in the activation of MAP kinases","volume":"528","author":"Son","year":"2013","journal-title":"Methods Enzymol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/0163-7258(89)90050-8","article-title":"Mechanisms of carbon tetrachloride toxicity","volume":"43","author":"Recknagel","year":"1989","journal-title":"Pharmacol. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1080\/713611034","article-title":"Hepatotoxicity and mechanism of action of haloalkanes: Carbon tetrachloride as a toxicological model","volume":"33","author":"Weber","year":"2003","journal-title":"Crit. Rev. Toxicol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1111\/j.1440-1746.2010.06592.x","article-title":"Reactive oxygen and mechanisms of inflammatory liver injury","volume":"26","author":"Jaeschke","year":"2011","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_13","first-page":"125","article-title":"Therapeutic uses of Ocimum sanctum Linn. (Tulsi) with a note on eugenol and its pharmacological actions: A short review","volume":"49","author":"Prakash","year":"2005","journal-title":"Indian J. Physiol. Pharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"95","DOI":"10.4103\/0973-7847.65323","article-title":"Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview","volume":"4","author":"Pattanayak","year":"2010","journal-title":"Pharmacogn. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0944-7113(00)80015-X","article-title":"Antioxidant and cyclooxygenase inhibitory phenolic compounds from Ocimum sanctum Linn","volume":"7","author":"Kelm","year":"2000","journal-title":"Phytomedicine"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/cr.2010.178","article-title":"Crosstalk of reactive oxygen species and NF-\u03baB signaling","volume":"21","author":"Morgan","year":"2011","journal-title":"Cell Res."},{"key":"ref_17","first-page":"465","article-title":"Effect of Ocimum sanctum (OS) leaf extract on hepatotoxicity induced by antitubercular drugs in rats","volume":"47","author":"Ubaid","year":"2003","journal-title":"Indian J. Physiol. Pharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/s13237-022-00405-3","article-title":"Plant-derived natural products for drug discovery: Current approaches and prospects","volume":"65","author":"Nasim","year":"2022","journal-title":"Nucleus"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1008151","DOI":"10.37349\/eds.2026.1008151","article-title":"Phytochemical profiles and therapeutic applications of selected medicinal plants: An evidence-based narrative review","volume":"4","author":"Bhandare","year":"2026","journal-title":"Explor. Drug Sci."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Makhloufi, H., Pinon, A., Champavier, Y., Saliba, J., Millot, M., Fruitier-Arnaudin, I., Liagre, B., Chemin, G., and Mambu, L. (2024). In Vitro Antiproliferative Activity of Echinulin Derivatives from Endolichenic Fungus Aspergillus sp. against Colorectal Cancer. Molecules, 29.","DOI":"10.3390\/molecules29174117"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Chung, C.L., Chen, J.H., Huang, W.C., Sheu, J.R., Hsia, C.W., Jayakumar, T., Hsia, C.H., Chiou, K.R., and Hou, S.M. (2022). Glabridin, a bioactive flavonoid from licorice, effectively inhibits platelet activation in humans and mice. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231911372"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Huang, W.C., Shu, L.H., Kuo, Y.J., Lai, K.S., Hsia, C.W., Yen, T.L., Hsia, C.H., Jayakumar, T., Yang, C.H., and Sheu, J.R. (2024). Eugenol suppresses platelet activation and mitigates pulmonary thromboembolism in humans and murine models. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25042098"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.33425\/2641-4295.1091","article-title":"Plant-derived methyl-eugenol as a selective modulator of platelet function and a potential cardiovascular protective agent","volume":"8","author":"Chang","year":"2025","journal-title":"Food Sci. Nutr. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"25999","DOI":"10.1021\/acsomega.3c02031","article-title":"Pleurospermum candollei methanolic extract ameliorates CCl4-induced liver injury by modulating oxidative stress, inflammatory, and apoptotic markers in rats","volume":"8","author":"Fatima","year":"2023","journal-title":"ACS Omega"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.jhep.2023.03.017","article-title":"Global burden of liver disease: 2023 update","volume":"79","author":"Devarbhavi","year":"2023","journal-title":"J. Hepatol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Ullah, H., Khan, A., Baig, M.W., Ullah, N., Ahmed, N., Tipu, M.K., Ali, H., and Khan, S. (2020). Poncirin attenuates CCl4-induced liver injury through inhibition of oxidative stress and inflammatory cytokines in mice. BMC Complement. Med. Ther., 20.","DOI":"10.1186\/s12906-020-02906-7"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/j.bbadis.2018.08.037","article-title":"Animal models of drug-induced liver injury","volume":"1865","author":"McGill","year":"2019","journal-title":"Biochim. Biophys. Acta Mol. Basis Dis."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Cheuka, P.M., Mayoka, G., Mutai, P., and Chibale, K. (2017). The role of natural products in drug discovery and development against neglected tropical diseases. Molecules, 22.","DOI":"10.3390\/molecules22010058"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/S0140-6736(99)00219-6","article-title":"Serum aminotransferase concentration as evidence of hepatocellular damage","volume":"355","author":"Kew","year":"2000","journal-title":"Lancet"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Chen, R., Lian, Y., Wen, S., Li, Q., Sun, L., Lai, X., Zhang, Z., Zhu, J., Tang, L., and Xuan, J. (2022). Shibi tea (Adinandra nitida) and Camellianin A alleviate CCl4-induced liver injury in C57BL\/6J mice by attenuation of oxidative stress, inflammation, and apoptosis. Nutrients, 14.","DOI":"10.3390\/nu14153037"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"106708","DOI":"10.1016\/j.fbio.2025.106708","article-title":"Hepatoprotective activity of Taraxacum officinale extract against CCl4-induced liver injury in rats","volume":"68","author":"Salim","year":"2025","journal-title":"Food Biosci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1016\/j.fct.2006.06.025","article-title":"Antioxidant activity of the oyster mushroom, Pleurotus ostreatus, on CCl4-induced liver injury in rats","volume":"44","author":"Jayakumar","year":"2006","journal-title":"Food Chem. Toxicol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Shaban, N.Z., El-Kot, S.M., Awad, O.M., Hafez, A.M., and Fouad, G.M. (2021). The antioxidant and anti-inflammatory effects of Carica papaya Linn. seeds extract on CCl4-induced liver injury in male rats. BMC Complement. Med. Ther., 21.","DOI":"10.1186\/s12906-021-03479-9"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"262","DOI":"10.5530\/pres.13.4.16","article-title":"Phytochemical and GC\u2013MS analysis of hydroethanolic leaf extract of Ocimum sanctum (L.)","volume":"13","author":"Srivastava","year":"2021","journal-title":"Pharmacogn. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biopha.2017.04.011","article-title":"Chemical composition of Ocimum sanctum by LC-ESI\u2013MS\/MS analysis and its protective effects against smoke-induced lung and neuronal tissue damage in rats","volume":"91","author":"Venuprasad","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"6177","DOI":"10.1021\/jm051256o","article-title":"Extra precision Glide: Docking and scoring incorporating a model of hydrophobic enclosure for protein\u2013ligand complexes","volume":"49","author":"Friesner","year":"2006","journal-title":"J. Med. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1021\/jm030644s","article-title":"Glide: A new approach for rapid, accurate docking and scoring. 2. Enrichment factors in database screening","volume":"47","author":"Halgren","year":"2004","journal-title":"J. Med. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1021\/jm0306430","article-title":"Glide: A new approach for rapid, accurate docking and scoring. 1. Method and assessment of docking accuracy","volume":"47","author":"Friesner","year":"2004","journal-title":"J. Med. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"8505","DOI":"10.1080\/07391102.2023.2246583","article-title":"Structure-based virtual screening of natural compounds against wild and mutant (R1155K, A1156T and D1168A) NS3-4A protease of hepatitis C virus","volume":"42","author":"Samantaray","year":"2024","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"W438","DOI":"10.1093\/nar\/gky439","article-title":"COACH-D: Improved protein\u2013ligand binding sites prediction with refined ligand-binding poses through molecular docking","volume":"46","author":"Wu","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"12696","DOI":"10.1021\/acs.jctc.5c01316","article-title":"Glide WS: Methodology and initial assessment of performance for docking accuracy and virtual screening","volume":"21","author":"Friesner","year":"2025","journal-title":"J. Chem. Theory Comput."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: High performance molecular simulations through multi-level parallelism from laptops to supercomputers","volume":"1\u20132","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1002\/jcc.20291","article-title":"GROMACS: Fast, flexible, and free","volume":"26","author":"Lindahl","year":"2005","journal-title":"J. Comput. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1021\/ct900549r","article-title":"Implementation of the CHARMM force field in GROMACS: Analysis of protein stability effects from correction maps, virtual interaction sites, and water models","volume":"6","author":"Bjelkmar","year":"2010","journal-title":"J. Chem. Theory Comput."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6469","DOI":"10.1021\/acs.jcim.3c01053","article-title":"SwissParam 2023: A modern web-based tool for efficient small molecule parametrization","volume":"63","author":"Bugnon","year":"2023","journal-title":"J. Chem. Inf. Model."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/j.1432-1033.1974.tb03714.x","article-title":"Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase","volume":"47","author":"Marklund","year":"1974","journal-title":"Eur. J. Biochem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1126\/science.179.4073.588","article-title":"Selenium: Biochemical role as a component of glutathione peroxidase","volume":"179","author":"Rotruck","year":"1973","journal-title":"Science"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/0003-2697(72)90132-7","article-title":"Colorimetric assay of catalase","volume":"47","author":"Sinha","year":"1972","journal-title":"Anal. Biochem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/0003-2697(71)90370-8","article-title":"Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels","volume":"44","author":"Beauchamp","year":"1971","journal-title":"Anal. Biochem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1002\/1522-2683(200202)23:4<513::AID-ELPS513>3.0.CO;2-J","article-title":"Activity staining of glutathione peroxidase after electrophoresis on native and sodium dodecyl sulfate polyacrylamide gels","volume":"23","author":"Lin","year":"2002","journal-title":"Electrophoresis"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/0003-2697(71)90375-7","article-title":"An improved procedure using ferricyanide for detecting catalase isozymes","volume":"44","author":"Woodbury","year":"1971","journal-title":"Anal. Biochem."},{"key":"ref_54","unstructured":"Drury, R.A.B., and Wallington, E.A. (1980). Carleton\u2019s Histological Technique, Churchill Livingstone. [5th ed.]."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/16\/2749\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T04:24:59Z","timestamp":1786249499000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/16\/2749"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,7]]},"references-count":54,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["molecules31162749"],"URL":"https:\/\/doi.org\/10.3390\/molecules31162749","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,7]]}}}