{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T17:16:08Z","timestamp":1786122968580,"version":"build-2736575974"},"reference-count":44,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009615","name":"Doctoral School of the University of Agriculture in Krak\u00f3w","doi-asserted-by":"publisher","award":["A726"],"award-info":[{"award-number":["A726"]}],"id":[{"id":"10.13039\/100009615","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009615","name":"Doctoral School of the University of Agriculture in Krak\u00f3w","doi-asserted-by":"publisher","award":["A738"],"award-info":[{"award-number":["A738"]}],"id":[{"id":"10.13039\/100009615","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009615","name":"Doctoral School of the University of Agriculture in Krak\u00f3w","doi-asserted-by":"publisher","award":["AD12"],"award-info":[{"award-number":["AD12"]}],"id":[{"id":"10.13039\/100009615","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Synthetic plastics remain widely used in food packaging despite environmental concerns. This laboratory-scale study evaluated biodegradable chitosan-alginate films enriched with oregano or turmeric two-phase equilibrium condensates as active packaging films for highbush blueberries (Vaccinium corymbosum L.). Film hydration properties were assessed, and blueberries were stored at 4 \u00b0C or 22 \u00b0C for 7 or 14 days. Dry matter, proximate composition, total phenolic content (TPC), antioxidant activity, vitamin C, and total viable bacterial count (TVC) were measured. Simulated gastrointestinal film digestates were evaluated in Nthy-ori 3-1 thyroid epithelial and CCD 841 CoN colonic epithelial cells. Oregano-enriched films (OS3) showed greater water uptake and swelling than control and turmeric-enriched films. Blueberry responses depended on formulation and storage conditions. After 14 days at 4 \u00b0C, OS2- and OS3-packaged blueberries showed higher vitamin C contents than plastic controls, corresponding to approximately 20% lower vitamin C loss. TVC was primarily influenced by temperature, with no significant main effect of packaging type. Digestates did not significantly alter evaluated DNA-damage markers under short-term exposure conditions. Concentration-dependent cell-cycle changes occurred in Nthy-ori 3-1 cells after OS2 and OS3 exposure, whereas no significant changes were observed in CCD-841CoN cells.<\/jats:p>","DOI":"10.3390\/molecules31162751","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T13:20:16Z","timestamp":1786108816000},"page":"2751","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioactive Chitosan-Alginate Films with Oregano or Turmeric: Effects on Blueberry Shelf Life and In Vitro Genotoxicity Profile"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9316-6760","authenticated-orcid":false,"given":"Aleksandra","family":"Such","sequence":"first","affiliation":[{"name":"Department of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in Krakow, Balicka 122, 30-149 Krakow, Poland"},{"name":"Department of Chemistry, Faculty of Food Technology, University of Agriculture in Krakow, Balicka 122, 30-149 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4940-3615","authenticated-orcid":false,"given":"Ewelina","family":"W\u0119sierska","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Public Health, Faculty of Veterinary Medicine, R\u0119dzina 1 C, 30-248 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8266-1619","authenticated-orcid":false,"given":"Magdalena","family":"Surma","sequence":"additional","affiliation":[{"name":"Department of Plant Products Technology and Nutrition Hygiene, Malopolska Centre of Food Monitoring, Faculty of Food Technology, University of Agriculture in Krakow, Balicka 122, 30-149 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Winiarz","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in Krakow, Balicka 122, 30-149 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1653-2867","authenticated-orcid":false,"given":"Aneta","family":"Koronowicz","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in Krakow, Balicka 122, 30-149 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.ijbiomac.2021.03.084","article-title":"Influence of Replacing Oregano Essential Oil by Ground Oregano Leaves on Chitosan\/Alginate-Based Dressings Properties","volume":"181","author":"Sobczyk","year":"2021","journal-title":"Int. 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