{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T08:30:44Z","timestamp":1786609844346,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Talent Introduction Plan of Xihua University","award":["ZX20250068"],"award-info":[{"award-number":["ZX20250068"]}]},{"award":["ZX20250068"],"award-info":[{"award-number":["ZX20250068"]}],"id":[{"id":"https:\/\/ror.org\/04gwtvf26","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Abelmoschus manihot (L.) flowers are widely used in traditional Chinese medicine, whereas the roots are often discarded as agricultural waste. The compact cellular and tissue architecture of the roots hinders the release of intracellular metabolites, thus impeding their reutilization. Here, enzyme-assisted ultrasonic extraction was developed to obtain a polysaccharide-rich crude extract from A. manihot roots. Orthogonal optimization identified pH 4.5, a temperature of 60 \u00b0C and a cellulase dosage of 6% (w\/w) as the optimal extraction conditions, giving an extraction yield of 21.59%. The phenol\u2013sulfuric acid assay indicated a total carbohydrate content of 75.09% (glucose equivalents). Structural analysis by Fourier transform infrared (FT\u2212IR) confirmed the presence of characteristic polysaccharide functional groups, while monosaccharide composition analysis via high-performance liquid chromatography (HPLC) revealed the major components of glucose, glucuronic acid, rhamnose, galacturonic acid, etc. Inspired by the traditional use of A. manihot extract in papermaking, its preliminary hair care-related properties were further evaluated. At a concentration of 30 mg\/mL, the extract solution inhibited Malassezia furfur growth by 84%, reduced wet and dry combing work by 16.93% and 30.86%, and increased tensile strength and tensile fracture energy by 13.04% and 14.84%, respectively. Scanning electron microscope (SEM) images suggested smoother hair fiber surfaces after treatment. These results highlight the extract\u2019s great potential for hair care cosmetic products.<\/jats:p>","DOI":"10.3390\/molecules31162817","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T08:06:16Z","timestamp":1786608376000},"page":"2817","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enzyme-Assisted Ultrasonic Extraction of a Polysaccharide-Rich Extract from Abelmoschus manihot (L.) Root and Preliminary Evaluation of Hair Care-Related Properties"],"prefix":"10.3390","volume":"31","author":[{"given":"Junjie","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Chemistry, School of Science, Xihua University, Chengdu 610039, China"},{"name":"Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Memorial Sun Yat-Sen), Nanjing 210014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Memorial Sun Yat-Sen), Nanjing 210014, China"},{"name":"Nanjing University of Chinese Medicine, Nanjing 210023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dian","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Memorial Sun Yat-Sen), Nanjing 210014, China"},{"name":"Nanjing University of Chinese Medicine, Nanjing 210023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Ren","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Memorial Sun Yat-Sen), Nanjing 210014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Science, Xihua University, Chengdu 610039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingqiong","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Science, Xihua University, Chengdu 610039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zenglai","family":"Xu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Memorial Sun Yat-Sen), Nanjing 210014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Memorial Sun Yat-Sen), Nanjing 210014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1038\/s41573-020-00114-z","article-title":"Natural products in drug discovery: Advances and opportunities","volume":"20","author":"Atanasov","year":"2021","journal-title":"Nat. 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