{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:30:33Z","timestamp":1786696233557,"version":"build-2736575974"},"reference-count":22,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007224","name":"National Foundation for Science and Technology Development","doi-asserted-by":"publisher","award":["106.02-2021.43"],"award-info":[{"award-number":["106.02-2021.43"]}],"id":[{"id":"10.13039\/100007224","id-type":"DOI","asserted-by":"publisher"}]},{"award":["106.02-2021.43"],"award-info":[{"award-number":["106.02-2021.43"]}],"id":[{"id":"https:\/\/ror.org\/04rw64z44","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Phyllanthus amarus has long been used in traditional medicine and contains diverse bioactive compounds with potential pharmacological properties. This study aimed to purify the compounds from P. amarus and investigate their inhibitory effects on mast cell degranulation in vitro. It was found that the ethyl acetate fraction from P. amarus possesses potential inhibitory effects on mast cell degranulation. Subsequently, three compounds, including 3,3\u2032,4-tri-O-methylellagic acid (P1), ethyl brevifolincarboxylate (P2), and 4\u2032,4\u2032\u2032\u2032-di-O-methyl cupressuflavone (P3), were purified and identified. These compounds significantly inhibited mast cell degranulation via decreasing histamine release and IL-4 and TNF-\u03b1 production from the activated mast cells without any cytotoxicity. Furthermore, Western blot analysis confirmed that these compounds suppressed phosphorylation of Syk and PLC\u03b3, suggesting inhibition of the degranulation-related signaling cascade. These findings provide preliminary in vitro evidence that purified constituents from P. amarus may inhibit mast cell degranulation, potentially in association with modulation of Syk\/PLC\u03b3 signaling, and support further mechanistic and in vivo investigations.<\/jats:p>","DOI":"10.3390\/molecules31162835","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:08:29Z","timestamp":1786694909000},"page":"2835","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Purified Compounds from Phyllanthus amarus Schum. &amp; Thonn. Inhibit Mast Cell Degranulation via Modulation of Syk\/PLC\u03b3 Signaling"],"prefix":"10.3390","volume":"31","author":[{"given":"Thanh Sang","family":"Vo","sequence":"first","affiliation":[{"name":"Center for Hi-Tech Development, Nguyen Tat Thanh University, Saigon Hi-Tech Park, Ho Chi Minh City 700000, Vietnam"},{"name":"Interdisciplinary Science Institute, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vo Thi Ngoc","family":"My","sequence":"additional","affiliation":[{"name":"Faculty of Medical Laboratory, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phan Van Hoai","family":"Luan","sequence":"additional","affiliation":[{"name":"Center for Hi-Tech Development, Nguyen Tat Thanh University, Saigon Hi-Tech Park, Ho Chi Minh City 700000, Vietnam"},{"name":"Interdisciplinary Science Institute, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4025-1694","authenticated-orcid":false,"given":"Dai-Hung","family":"Ngo","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Thu Dau Mot University, Ho Chi Minh City 750000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.jep.2011.09.040","article-title":"Phyllanthus amarus: Ethnomedicinal uses, phytochemistry and pharmacology: A review","volume":"138","author":"Patel","year":"2011","journal-title":"J. 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