{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:25:20Z","timestamp":1787030720531,"version":"build-2736575974"},"reference-count":70,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:00:00Z","timestamp":1786752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o Carlos Chagas de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro, Brazil","award":["E26\/210.537\/2025"],"award-info":[{"award-number":["E26\/210.537\/2025"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro, Brazil","award":["E-26\/202.101\/2025"],"award-info":[{"award-number":["E-26\/202.101\/2025"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro, Brazil","award":["E-26\/203.745\/2024"],"award-info":[{"award-number":["E-26\/203.745\/2024"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro, Brazil","award":["E-26\/200.891\/2021"],"award-info":[{"award-number":["E-26\/200.891\/2021"]}]},{"award":["E26\/210.537\/2025"],"award-info":[{"award-number":["E26\/210.537\/2025"]}],"id":[{"id":"https:\/\/ror.org\/051hcjr97","id-type":"ROR","asserted-by":"publisher"}]},{"award":["E-26\/202.101\/2025"],"award-info":[{"award-number":["E-26\/202.101\/2025"]}],"id":[{"id":"https:\/\/ror.org\/051hcjr97","id-type":"ROR","asserted-by":"publisher"}]},{"award":["E-26\/203.745\/2024"],"award-info":[{"award-number":["E-26\/203.745\/2024"]}],"id":[{"id":"https:\/\/ror.org\/051hcjr97","id-type":"ROR","asserted-by":"publisher"}]},{"award":["E-26\/200.891\/2021"],"award-info":[{"award-number":["E-26\/200.891\/2021"]}],"id":[{"id":"https:\/\/ror.org\/051hcjr97","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["313119\/2020-1"],"award-info":[{"award-number":["313119\/2020-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"award":["313119\/2020-1"],"award-info":[{"award-number":["313119\/2020-1"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES) Brazil","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"https:\/\/ror.org\/00x0ma614","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Pleurotus ostreatus (shimeji) is an edible mushroom widely recognized for its nutritional value and production of bioactive compounds. In addition to its fruiting body, mycelial biomass has emerged as a promising and sustainable alternative to produce bioactive compounds under controlled cultivation conditions. This study evaluated the nutritional composition, texture profile, biochemical composition, phenolic compounds, and antioxidant properties of the mycelium of P. ostreatus. Nutritional parameters were determined by FoodScan\u2122 (NIR), while biochemical composition was assessed through spectrophotometric quantification of soluble proteins, free amino acids, reducing sugars, and total carbohydrates. Texture Profile Analysis (TPA) was performed to characterize the mechanical and technological properties of the mycelial biomass. Total phenolic compounds were quantified using the Folin\u2013Ciocalteu method, and individual phenolics were identified by HPLC-DAD. Antioxidant activity was evaluated by ABTS, DPPH, TEAC, and hydroxyl radical scavenging assays. The mycelium exhibited high moisture content and relevant protein levels, as well as a rigid structural profile characterized by elevated hardness and gumminess. Biochemical characterization revealed considerable concentrations of soluble proteins and free amino acids, together with detectable levels of reducing sugars and total carbohydrates. The aqueous extract showed high total phenolic content and expressive antioxidant activity, with gallic acid identified as the predominant phenolic compound, followed by ferulic acid. The extracts also demonstrated efficient radical scavenging capacity in ABTS, DPPH, and hydroxyl radical assays. These findings highlight P. ostreatus mycelium as a sustainable source of bioactive compounds with potential applications as a functional ingredient in food, nutraceutical, and biotechnology sectors, reinforcing its role within circular bioeconomy strategies.<\/jats:p>","DOI":"10.3390\/molecules31162851","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T10:04:36Z","timestamp":1786961076000},"page":"2851","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biochemical Composition, Nutritional Profile, Bioactive Compound Content, Antioxidant Activity, and Technological Potential of Mycelium from Pleurotus ostreatus (Shimeji)"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6953-4201","authenticated-orcid":false,"given":"Ygor Velloso","family":"Tavares","sequence":"first","affiliation":[{"name":"Analytical and Molecular Laboratorial Center (CLAn), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-598, RJ, Brazil"},{"name":"Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Graduate Program in Biochemistry (PPGBq), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davi Vieira Teixeira","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Graduate Program in Food Science (PPGCAL), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz","family":"Torres Neto","sequence":"additional","affiliation":[{"name":"Analytical and Molecular Laboratorial Center (CLAn), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-598, RJ, Brazil"},{"name":"Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Graduate Program in Food Science (PPGCAL), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana J\u00falia Bento","family":"do Amaral","sequence":"additional","affiliation":[{"name":"Analytical and Molecular Laboratorial Center (CLAn), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-598, RJ, Brazil"},{"name":"Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Graduate Program in Food Science (PPGCAL), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2585-3336","authenticated-orcid":false,"given":"Adaelson Firmino","family":"da Silva Junior","sequence":"additional","affiliation":[{"name":"Analytical and Molecular Laboratorial Center (CLAn), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-598, RJ, Brazil"},{"name":"Laboratory of Advanced Analysis in Biochemistry and Molecular Biology 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Brazil"},{"name":"Graduate Program in Biochemistry (PPGBq), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Graduate Program in Food Science (PPGCAL), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Graduate Program in Chemistry (PGQu), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), Cidade Universit\u00e1ria, Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Department of Pharmaceutical Technology, Federal Fluminense University (UFF), Dr. Mario Vianna Street, 523, Niter\u00f3i 24210-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7337-2679","authenticated-orcid":false,"given":"Rosane Marina","family":"Peralta","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, State 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