{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T04:27:27Z","timestamp":1787113647330,"version":"build-2736575974"},"reference-count":83,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003069","name":"Instituto Polit\u00e9cnico Nacional","doi-asserted-by":"publisher","award":["SIP-IN-20260540"],"award-info":[{"award-number":["SIP-IN-20260540"]}],"id":[{"id":"10.13039\/501100003069","id-type":"DOI","asserted-by":"publisher"}]},{"award":["SIP-IN-20260540"],"award-info":[{"award-number":["SIP-IN-20260540"]}],"id":[{"id":"https:\/\/ror.org\/059sp8j34","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Tilia americana var. mexicana is a medicinal species whose reported pharmacological effects primarily target the central nervous system, including anxiolytic, antidepressant, and anticonvulsant activities, attributed in part to flavonoids such as tiliroside (8). In this study, 14 compounds were isolated and identified from the methanolic extract (Ta-MeOH) and evaluated by molecular docking against the monoamine oxidases MAO-A and MAO-B, enzymes implicated in Parkinson\u2019s disease (PD) due to their involvement in dopamine metabolism. Chemical analysis identified six terpenes: \u03b1- and \u03b2-amyrine (1\u20132), \u03b2-sitosterol (3), stigmasterol (4), ursolic acid (5), and \u03b2-sitosterol glucoside (6); the novel diglycosylated monoterpene 4\u03b1-terpineol sambubioside (7), characterized through its hexaacetate derivative (7a); the flavonoids tiliroside (8) and rutin (9); sucrose (10); and four phenolic compounds: scopoletin (11), caffeic acid (12), coumaric acid (13), and chlorogenic acid (14). Molecular docking against monoamine oxidases A and B (MAO-A and MAO-B) was used as a computational strategy to prioritize isolated metabolites for biological evaluation. \u03b2-Sitosterol glucoside (6), 4\u03b1-terpineol sambubioside (7), tiliroside (8) and rutin (9) exhibited the most favorable docking scores toward MAO-B, with predicted binding energies of \u221210.8733, \u221211.1434, \u221212.16986 and \u221212.9474 kcal\/mol, respectively. Considering the docking results together with phytochemical and experimental criteria, (6) and (8) (1 mg\/kg) were prioritized for biological evaluation, together with Ta-MeOH extract (100 mg\/kg) and selected fractions (25 mg\/kg), in a reserpine-induced mouse model of parkinsonism using L-DOPA (150 mg\/kg) as a positive control. In the open field test, spontaneous locomotor activity was assessed by total crossings and expressed as AUC. Ta-MeOH significantly increased locomotor activity compared with the reserpine-treated group (AUC: 1281.8 vs. 551.7, respectively; p &lt; 0.05). Tiliroside (8) also increased locomotor activity (AUC: 858). In the Rota-Rod test, fine motor coordination was assessed by latency to fall and expressed as AUC. Ta-MeOH showed the greatest recovery, with AUC values of 431, 89, and 34 at 4, 10, and 20 rpm, respectively. In addition, treatments derived from T. americana attenuated behavioral alterations induced by reserpine in the Irwin test. Overall, this study demonstrates that integrating phytochemical isolation, molecular docking, and in vivo pharmacological evaluation provides a useful strategy for prioritizing bioactive metabolites from T. americana. These findings support further pharmacological investigation of this medicinal species and its isolated metabolites, while additional studies are required to establish their molecular targets and mechanisms of action.<\/jats:p>","DOI":"10.3390\/molecules31162874","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:35:08Z","timestamp":1786984508000},"page":"2874","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Characterization of Tilia americana var. mexicana, Exploratory Study of MAO Molecular Docking and Evaluation of Behavior in Mice in a Reserpine-Induced Parkinson Model"],"prefix":"10.3390","volume":"31","author":[{"given":"Maribel","family":"Osorio-Garc\u00eda","sequence":"first","affiliation":[{"name":"Centro de Desarrollo de Productos Bi\u00f3ticos, Instituto Polit\u00e9cnico Nacional, Carr. Yautepec-Jojutla s\/n-km. 85, San Isidro, Yautepec 62739, Morelos, Mexico"},{"name":"Facultad de Ciencias Qu\u00edmicas e Ingenier\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7748-4432","authenticated-orcid":false,"given":"Antonio Ruperto","family":"Jim\u00e9nez-Aparicio","sequence":"additional","affiliation":[{"name":"Centro de Desarrollo de Productos Bi\u00f3ticos, Instituto Polit\u00e9cnico Nacional, Carr. Yautepec-Jojutla s\/n-km. 85, San Isidro, Yautepec 62739, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9056-3568","authenticated-orcid":false,"given":"Maribel","family":"Herrera-Ruiz","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0783-8274","authenticated-orcid":false,"given":"Enrique","family":"Jim\u00e9nez-Ferrer","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2233-5958","authenticated-orcid":false,"given":"Alejandro","family":"Zamilpa","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca Eda","family":"Dom\u00ednguez-Mendoza","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7479-9332","authenticated-orcid":false,"given":"Gabriela","family":"Trejo-Tapia","sequence":"additional","affiliation":[{"name":"Centro de Desarrollo de Productos Bi\u00f3ticos, Instituto Polit\u00e9cnico Nacional, Carr. Yautepec-Jojutla s\/n-km. 85, San Isidro, Yautepec 62739, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3693-1670","authenticated-orcid":false,"given":"Manas\u00e9s","family":"Gonz\u00e1lez-Cortazar","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1007\/s00702-017-1686-y","article-title":"Epidemiology of Parkinson\u2019s disease","volume":"124","author":"Tysnes","year":"2017","journal-title":"J. Neural Transm."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"17013","DOI":"10.1038\/nrdp.2017.13","article-title":"Parkinson disease","volume":"3","author":"Poewe","year":"2017","journal-title":"Nat. Rev. Dis. Prim."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1016\/S0140-6736(14)61393-3","article-title":"Parkinson\u2019s disease","volume":"386","author":"Kalia","year":"2015","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1001\/jama.2019.22360","article-title":"Diagnosis and Treatment of Parkinson Disease: A Review","volume":"323","author":"Armstrong","year":"2020","journal-title":"JAMA"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2284","DOI":"10.1016\/S0140-6736(21)00218-X","article-title":"Parkinson\u2019s disease","volume":"397","author":"Bloem","year":"2021","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/jnc.13731","article-title":"Mitochondrial dysfunction in Parkinson\u2019s disease","volume":"139","author":"Bose","year":"2016","journal-title":"J. Neurochem."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Khan, M.S., Nasiripour, S., and Bopassa, J.C. (2025). Parkinson Disease Signaling Pathways, Molecular Mechanisms, and Potential Therapeutic Strategies: A Comprehensive Review. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26136416"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1146\/annurev.neuro.22.1.197","article-title":"Monoamine oxidase: From genes to behavior","volume":"22","author":"Shih","year":"1999","journal-title":"Annu. Rev. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"101957","DOI":"10.1016\/j.jchemneu.2021.101957","article-title":"The role of monoamine oxidase enzymes in the pathophysiology of neurological disorders","volume":"114","author":"Jones","year":"2021","journal-title":"J. Chem. Neuroanat."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"112787","DOI":"10.1016\/j.ejmech.2020.112787","article-title":"A comprehensive review of monoamine oxidase inhibitors as Anti-Alzheimer\u2019s disease agents: A review","volume":"206","author":"Manzoor","year":"2020","journal-title":"Eur. J. Med. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"100274","DOI":"10.1016\/j.mocell.2025.100274","article-title":"Current therapeutic strategies in Parkinson\u2019s disease: Future perspectives","volume":"48","year":"2025","journal-title":"Mol. Cells."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"425","DOI":"10.2174\/1871527316666170124165222","article-title":"Monoamine Oxidase B Inhibitors in Parkinson\u2019s Disease","volume":"16","author":"Dezsi","year":"2017","journal-title":"CNS Neurol. Disord. Drug Targets"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5607\/en.2011.20.1.1","article-title":"MAO-inhibitors in Parkinson\u2019s Disease","volume":"20","author":"Riederer","year":"2011","journal-title":"Exp. Neurobiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2799","DOI":"10.2174\/138161210793176527","article-title":"Monoamine oxidase inhibitors as neuroprotective agents in age-dependent neurodegenerative disorders","volume":"16","author":"Naoi","year":"2010","journal-title":"Curr. Pharm. Des."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"115410","DOI":"10.1016\/j.bbr.2024.115410","article-title":"Effects of MAO-B inhibitors in life quality of Parkinson\u2019s disease patients: A systematic review and meta-analysis","volume":"480","author":"Xiaohuan","year":"2025","journal-title":"Behav. Brain Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1038\/s41531-022-00339-2","article-title":"Effects of MAO-B inhibitors on non-motor symptoms and quality of life in Parkinson\u2019s disease: A systematic review","volume":"8","author":"Tsuboi","year":"2022","journal-title":"npj Park. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"81","DOI":"10.4103\/0973-7847.99898","article-title":"Neuroprotective potential of phytochemicals","volume":"6","author":"Kumar","year":"2012","journal-title":"Pharmacogn. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"814068","DOI":"10.1155\/2015\/814068","article-title":"Phytochemicals that regulate neurodegenerative disease by targeting neurotrophins: A comprehensive review","volume":"2015","author":"Venkatesan","year":"2015","journal-title":"Biomed. Res. Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0378-8741(94)90098-1","article-title":"Isolation of pharmacologically active benzodiazepine receptor ligands from Tilia tomentosa (Tiliaceae)","volume":"44","author":"Viola","year":"1994","journal-title":"J. Ethnopharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"9420035","DOI":"10.26599\/FMH.2025.9420035","article-title":"Comprehensive review of Tilia L.: Phytochemical profiles, edible value, therapeutic potentials, and ecological significance","volume":"2","author":"Zhou","year":"2025","journal-title":"Food Med. Homol."},{"key":"ref_21","first-page":"1337","article-title":"Atlas de las plantas de la medicina tradicional mexicana, I, II & III","volume":"175","author":"Argueta","year":"1994","journal-title":"Inst. Nac. Indig."},{"key":"ref_22","first-page":"113","article-title":"An endangered and potentially economic tree of Mexico: Tilia mexicana (Tiliaceae)","volume":"54","author":"Pavon","year":"2000","journal-title":"Econ. Bot."},{"key":"ref_23","first-page":"329172","article-title":"Anticonvulsant and antioxidant effects of Tilia americana var. mexicana and flavonoids constituents in the pentylenetetrazole-induced seizures","volume":"2014","author":"Sampieri","year":"2014","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.jep.2008.04.019","article-title":"Flavonoids from Tilia americana with anxiolytic activity in the plus-maze test","volume":"118","author":"Zamilpa","year":"2008","journal-title":"J. Ethnopharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1055\/s-2007-981593","article-title":"Bioactivity-guided isolation of beta-sitosterol and some fatty acids as active compounds in the anxiolytic and sedative effects of Tilia americana var. mexicana","volume":"73","author":"Moreno","year":"2007","journal-title":"Planta Medica"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.jep.2009.09.044","article-title":"HPLC\/MS analysis and anxiolytic-like effect of quercetin and kaempferol flavonoids from Tilia americana var. mexicana","volume":"127","author":"Moreno","year":"2010","journal-title":"J. Ethnopharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.jep.2015.01.029","article-title":"Interactions of a standardized flavonoid fraction from Tilia americana with serotoninergic drugs in the elevated plus maze","volume":"164","author":"Zamilpa","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_28","first-page":"1931","article-title":"Effect of standardized fractions and tiliroside from leaves of Tilia americana on depression tests in mice","volume":"18","author":"Tortoriello","year":"2019","journal-title":"Iran. J. Pharm. Res."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Ullah, A., Munir, S., Badshah, S.L., Khan, N., Ghani, L., Poulson, B.G., Emwas, A.H., and Jaremko, M. (2022). Important flavonoids and their role as therapeutic agents. Molecules, 25.","DOI":"10.3390\/molecules25225243"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Bellavite, P. (2023). Neuroprotective Potentials of Flavonoids: Experimental Studies and Mechanisms of Action. Antioxidants, 12.","DOI":"10.3390\/antiox12020280"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ayaz, M., Sadiq, A., Junaid, M., Ullah, F., Ovais, M., Ullah, I., Ahmed, J., and Shahid, M. (2019). Flavonoids as Prospective Neuroprotectants and Their Therapeutic Propensity in Aging Associated Neurological Disorders. Front. Aging Neurosci., 11.","DOI":"10.3389\/fnagi.2019.00155"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.2174\/1568026614666140929124445","article-title":"Structure-based virtual screening for drug discovery: Principles, applications and recent advances","volume":"14","author":"Lionta","year":"2014","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"13384","DOI":"10.3390\/molecules200713384","article-title":"Molecular docking and structure-based drug design strategies","volume":"20","author":"Ferreira","year":"2015","journal-title":"Molecules"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"133444","DOI":"10.1016\/j.molstruc.2022.133444","article-title":"Synthesis, molecular modeling, 3D-QSAR and biological evaluation studies of new benzimidazole derivatives as potential MAO-A and MAO-B inhibitors","volume":"1265","author":"Erol","year":"2022","journal-title":"J. Mol. Struct."},{"key":"ref_35","first-page":"033","article-title":"Terpenoids and Sterols from Hoya multiflora Blume","volume":"5","author":"Ebajo","year":"2015","journal-title":"J. App Pharm. Sci."},{"key":"ref_36","first-page":"399","article-title":"Chemical constituents from the roots of Calotropis procera (AIT) R. BR","volume":"3","author":"Mohammed","year":"2016","journal-title":"Eur. J. Biomed. Pharm. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.steroids.2003.09.012","article-title":"Gradient enhanced selective experiments in the 1H NMR chemical shift assignment of the skeleton and side-chain resonances of stigmasterol, a phytosterol derivative","volume":"69","author":"Forgo","year":"2004","journal-title":"Steroids"},{"key":"ref_38","first-page":"303","article-title":"Estudio fitoqu\u00edmico de hojas de Uncaria guianensis y evaluaci\u00f3n de actividad antibacteriana","volume":"41","author":"Patino","year":"2010","journal-title":"Acta Amaz."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1002\/mrc.1214","article-title":"Complete assignments of 1H and 13C NMR resonances of oleanolic acid, 18a-oleanolic acid, ursolic acid and their 11-oxo derivatives","volume":"41","author":"Seebacher","year":"2003","journal-title":"Magn. Reson. Chem."},{"key":"ref_40","first-page":"1","article-title":"Isolation and Characterization of \u03b2-Sitosterol-3-O-\u03b2-D-glucoside from the Extract of the Flowers of Viola odorata","volume":"16","author":"Peshin","year":"2017","journal-title":"J. Pharm. Res. Int."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1002\/cbdv.201100350","article-title":"Glycosidic inhibitors of melanogenesis from leaves of Momordica charantia","volume":"9","author":"Kikuchi","year":"2012","journal-title":"Chem. Biodivers."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1111\/1541-4337.12389","article-title":"Review on the Dietary Flavonoid Tiliroside","volume":"17","author":"Grochowski","year":"2018","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_43","first-page":"206","article-title":"Isolation and 1H-NMR identification of a tiliroside from Odontonema strictum (Acanthaceae)","volume":"5","author":"Lokadi","year":"2016","journal-title":"J. Pharmacogn. Phytochem."},{"key":"ref_44","first-page":"222","article-title":"Extraction of Flavonoids and Quantification of Rutin from waste Tobacco Leaves","volume":"3","author":"Fathiazad","year":"2006","journal-title":"Iran. J. Pharm. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.dib.2018.12.008","article-title":"Nuclear magnetic resonance spectroscopy data of isolated compounds from Acacia farnesiana (L) Willd fruits and two esterified derivatives","volume":"22","year":"2019","journal-title":"Data Brief."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Gao, X.Y., Li, X.Y., Zhang, C.Y., and Bai, C.Y. (2024). Scopoletin: A review of its pharmacology, pharmacokinetics, and toxicity. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1268464"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Yu, J., Xie, J., Sun, M., Xiong, S., Xu, C., Zhang, Z., Li, M., Li, C., and Lin, L. (2024). Plant-Derived Caffeic Acid and Its Derivatives: An Overview of Their NMR Data and Biosynthetic Pathways. Molecules, 29.","DOI":"10.3390\/molecules29071625"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3146","DOI":"10.3390\/molecules20023146","article-title":"Spectroscopic (FT-IR, FT-Raman, 1H-and 13C-NMR), Theoretical and Microbiological Study of trans o-Coumaric Acid and Alkali Metal o-Coumarates","volume":"20","author":"Lewandowska","year":"2015","journal-title":"Molecules"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S0021-9673(01)90742-3","article-title":"Chromatographic separation and identification of naturally occurring chlorogenic acids by 1H nuclear magnetic resonance spectroscopy and mass spectrometry","volume":"315","author":"Morishita","year":"1984","journal-title":"J. Chromatogr."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1002\/(SICI)1099-1565(199807\/08)9:4<177::AID-PCA404>3.0.CO;2-3","article-title":"Structure assignment of natural quinic acid derivatives using proton nuclear magnetic resonance techniques","volume":"9","author":"Pauli","year":"1998","journal-title":"Phytochem. Anal."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"128888","DOI":"10.1016\/j.foodchem.2020.128888","article-title":"Nutlets of Tilia cordata Mill. and Tilia platyphyllos Scop\u2014Source of bioactive compounds","volume":"346","author":"Siger","year":"2021","journal-title":"Food Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.neuropharm.2008.10.010","article-title":"Antinociceptive activity of Tilia americana var. mexicana inflorescences and quercetin in the formalin test and in an arthritic pain model in rats","volume":"56","author":"Moreno","year":"2009","journal-title":"Neuropharmacology"},{"key":"ref_53","first-page":"7836820","article-title":"Hypoglycemic Activity of Tilia americana, Borago officinalis, Chenopodium nuttalliae, and Piper sanctum on Wistar Rats","volume":"2019","year":"2019","journal-title":"J. Diabetes Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"113619","DOI":"10.1016\/j.jep.2020.113619","article-title":"A mixture of quercetin 4\u2019-O-rhamnoside and isoquercitrin from Tilia americana var. mexicana and its biotransformation products with antidepressant activity in mice","volume":"267","author":"Zamilpa","year":"2021","journal-title":"J.-Ethnopharmacol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Ju\u00e1rez Ram\u00edrez, V.A., Jim\u00e9nez-Beltr\u00e1n, M.I., Zamilpa, A., Herrera-Ruiz, M., Abarca-Vargas, R., Lombardo-Earl, G., Tortoriello, J., and Jim\u00e9nez-Ferrer, E. (2017). Pharmacokinetic Study of Biotransformation Products from an Anxiolytic Fraction of Tilia americana. Molecules, 22.","DOI":"10.3390\/molecules22081260"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1080\/14756366.2020.1800666","article-title":"Discovery of N-(1-(3-fluorobenzoyl)-1H-indol-5-yl)pyrazine-2-carboxamide: A novel, selective, and competitive indole-based lead inhibitor for human monoamine oxidase B","volume":"35","author":"Elkamhawy","year":"2020","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_57","first-page":"687","article-title":"Computational investigation of unsaturated ketone derivatives as MAO-B inhibitors by using QSAR, ADME\/Tox, molecular docking, and molecular dynamics simulations","volume":"46","author":"Bouachrine","year":"2021","journal-title":"Turk. J. Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1248\/cpb.c23-00763","article-title":"Synthesis and Biological Evaluation of 2-Azolylmethylene-3-(2H)-benzofuranone Derivatives as Potent Monoamine Oxidases Inhibitors","volume":"72","author":"Takao","year":"2024","journal-title":"Chem. Pharm. Bull."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"10230","DOI":"10.1080\/07391102.2021.1940286","article-title":"In-silico evaluations of the isolated phytosterols from polygonum hydropiper L against BACE1 and MAO drug targets","volume":"40","author":"Ayaz","year":"2022","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1007\/s00213-015-4118-4","article-title":"Behavioral and neurochemical effects induced by reserpine in mice","volume":"233","author":"Busanello","year":"2016","journal-title":"Psychopharmacology"},{"key":"ref_61","first-page":"49","article-title":"Behavioral analysis of the reserpine induced motor changes in a Parkinsonian mouse model","volume":"11","author":"Carrillo","year":"2011","journal-title":"Rev. Neuropsicol. Neuropsiquiatr. Neurocienc."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1037\/h0043788","article-title":"The relation between fear induced by novel stimulation and exploratory behavior","volume":"48","author":"Montgomery","year":"1955","journal-title":"J. Comp. Physiol. Psychol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/0091-3057(88)90413-3","article-title":"Thigmotaxis as a test for anxiolytic activity in rats","volume":"31","author":"Treit","year":"1988","journal-title":"Pharmacol. Biochem. Behav."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0014-2999(03)01272-X","article-title":"The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: A review","volume":"463","author":"Prut","year":"2003","journal-title":"Eur. J. Pharmacol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Sheta, R., B\u00e9rard, M., Musiol, D., Mart\u00ednez-Drudis, L., and Oueslati, A. (2024). Behavioral analysis of motor and non-motor impairment in rodent models of Parkinson\u2019s disease. Front. Aging Neurosci., 16.","DOI":"10.3389\/fnagi.2024.1464706"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"10544","DOI":"10.1038\/s41598-022-14823-5","article-title":"Neurogenic effects of rotarod walking exercise in subventricular zone, subgranular zone, and substantia nigra in MPTP-induced Parkinson\u2019s disease mice","volume":"12","author":"Leem","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2340","DOI":"10.1080\/14786419.2012.654608","article-title":"Anti-inflammatory effects of \u03b2-sitosterol-\u03b2-D-glucoside from Trachelospermum jasminoides (Apocynaceae) in lipopolysaccharide-stimulated RAW 264.7 murine macrophages","volume":"26","author":"Choi","year":"2012","journal-title":"Nat. Prod. Res."},{"key":"ref_68","first-page":"9","article-title":"Evaluation of Antimicrobial and Antioxidant Activity of \u03b2-Sitosterol-3-O-Glucoside Isolated from Lannea kerstingii Engl. & K. Krause (Anacardiaceae)","volume":"2","author":"Njinga","year":"2016","journal-title":"Int. J. Sci. Glob. Sustain."},{"key":"ref_69","first-page":"45","article-title":"\u03b2-Sitosterol \u03b2-D-glucoside (BSSG) triggers intestinal inflammation in zebrafish and mouse models prior to neurodegeneration onset","volume":"33","author":"Terrin","year":"2026","journal-title":"Int. J. Biomed. Sci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1038\/s41398-025-03312-8","article-title":"Subtle behavioral alterations in the spontaneous behaviors of MPTP mouse model of Parkinson\u2019s disease","volume":"15","author":"Zhong","year":"2025","journal-title":"Transl. Psychiatry"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.pathophys.2018.12.002","article-title":"\u0392eta-sitosterol enhances motor coordination, attenuates memory loss and demyelination in a vanadium-induced model of experimental neurotoxicity","volume":"26","author":"Adebiyi","year":"2019","journal-title":"Pathophysiology"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Alkholifi, F.K., Devi, S., Aldawsari, M.F., Foudah, A.I., Alqarni, M.H., Salkini, M.A., and Sweilam, S.H. (2023). Effects of Tiliroside and Lisuride Co-Treatment on thePI3K\/Akt Signal Pathway: Modulating Neuroinflammation and Apoptosis in Parkinson\u2019s Disease. Biomedicines, 11.","DOI":"10.3390\/biomedicines11102735"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0014-2999(02)02953-9","article-title":"Assessment of the anti-inflammatory activity and free radical scavenger activity of tiliroside","volume":"461","author":"Sala","year":"2003","journal-title":"Eur. J. Pharmacol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"3311","DOI":"10.1016\/j.bbagen.2014.08.008","article-title":"Tiliroside, a dietary glycosidic flavonoid, inhibits TRAF-6\/NF-\u03baB\/p38-mediated neuroinflammation in activated BV2 microglia","volume":"1840","author":"Velagapudi","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Cruz-Castillo, J.D., Gonz\u00e1lez-Cortazar, M., Hern\u00e1ndez-Hern\u00e1ndez, P., Zamilpa, A., Guti\u00e9rrez-Rom\u00e1n, A.S., G\u00f3mez-Rivera, A., L\u00f3pez-Rodr\u00edguez, R., Ruiz-Ramos, D., Nolasco-Rosales, G.A., and Tovilla-Z\u00e1rate, C.A. (2026). Phytochemical Analysis, Antioxidant Activity, and Inhibition of Digestive Enzymes of Carica papaya L. Leaf. Molecules, 31.","DOI":"10.3390\/molecules31132394"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"5739","DOI":"10.1073\/pnas.0710626105","article-title":"Structure of human monoamine oxidase A at 2.2-A resolution: The control of opening the entry for substrates\/inhibitors","volume":"105","author":"Son","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"5848","DOI":"10.1021\/jm070677y","article-title":"Structures of human monoamine oxidase B complexes with selective noncovalent inhibitors: Safinamide and coumarin analogs","volume":"50","author":"Binda","year":"2007","journal-title":"J. Med. Chem."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1021\/jm031087c","article-title":"Crystal structures of monoamine oxidase B in complex with four inhibitors of the N-propargylaminoindan class","volume":"47","author":"Binda","year":"2004","journal-title":"J. Med. Chem."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"609","DOI":"10.29356\/jmcs.v68i4.2284","article-title":"Design and Synthesis of Barbiturates and Hydantoins with Multitarget Antidiabetic Effect","volume":"68","year":"2024","journal-title":"J. Mex. Chem. Soc."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2439","DOI":"10.1007\/s00210-020-01923-0","article-title":"Effects of CATECHIN on reserpine-induced vacuous chewing movements: Behavioral and biochemical analysis","volume":"393","author":"Reinheimer","year":"2020","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Hubrecht, R.C., and Carter, E. (2019). The 3Rs and Humane Experimental Technique: Implementing Change. Animals, 9.","DOI":"10.3390\/ani9100754"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/BF02250921","article-title":"Role of D1 receptor mechanisms in the potentiation of motor responses to L-DOPA and apomorphine by MK 801 in the reserpine-treated mouse","volume":"8","author":"Kaur","year":"1994","journal-title":"J. Neural Transm. Gen. Sect."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"15185","DOI":"10.1038\/s41598-021-94175-8","article-title":"Dopamine-loaded nanoparticle systems circumvent the blood-brain barrier restoring motor function in mouse model for Parkinson\u2019s Disease","volume":"11","author":"Lima","year":"2021","journal-title":"Sci. Rep."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/16\/2874\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T04:15:09Z","timestamp":1787112909000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/16\/2874"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":83,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["molecules31162874"],"URL":"https:\/\/doi.org\/10.3390\/molecules31162874","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]}}}