{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T09:06:59Z","timestamp":1789117619417,"version":"build-2803163510"},"reference-count":38,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"General Directorate of Academic Personnel Affairs, National Autonomous University of Mexico","award":["PAPIIT IN222023"],"award-info":[{"award-number":["PAPIIT IN222023"]}]},{"name":"General Directorate of Academic Personnel Affairs, National Autonomous University of Mexico","award":["IA205324"],"award-info":[{"award-number":["IA205324"]}]},{"award":["PAPIIT IN222023"],"award-info":[{"award-number":["PAPIIT IN222023"]}],"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}]},{"award":["IA205324"],"award-info":[{"award-number":["IA205324"]}],"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Chronic myeloid leukemia (CML) is driven by constitutive BCR\u2013ABL1 activity and remains clinically challenging due to disease persistence and resistance to tyrosine kinase inhibitors (TKIs). SIRT1, a NAD+-dependent deacetylase, has been implicated in leukemic cell survival and chemoresistance through negative regulation of p53. In this study, we evaluated the antileukemic effects of the Sechium hybrid H387 extract and its impact on the SIRT1\u2013p53 signaling axis in K562 CML cells. Cell proliferation was assessed using a crystal violet assay, while gene and protein expression were analyzed by RT-qPCR, immunofluorescence, and flow cytometry. Apoptosis was determined by Annexin V\/7-AAD staining. The extract significantly inhibited K562 cell proliferation and downregulated SIRT1 expression, reducing its nuclear localization. These effects were associated with increased p53 acetylation at lysine 382 (Ac-K382-p53) and enhanced apoptosis. Notably, a higher percentage of apoptotic cells was observed following extract treatment than with imatinib under the experimental conditions used, although the two agents have distinct mechanisms of action. These findings suggest that the antileukemic activity of Sechium hybrid H387 extract is associated with modulation of the SIRT1\u2013p53 axis and induction of apoptosis in K562 cells.<\/jats:p>","DOI":"10.3390\/molecules31172998","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T08:02:27Z","timestamp":1787817747000},"page":"2998","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antileukemic Activity of Sechium Hybrid H387 Extract Associated with SIRT1 Downregulation and p53 Acetylation in Human Chronic Myeloid Leukemia Cells"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1692-8287","authenticated-orcid":false,"given":"Itzen","family":"Agui\u00f1iga-S\u00e1nchez","sequence":"first","affiliation":[{"name":"Hematopoiesis and Leukemia Laboratory, Research Unit on Cell Differentiation and Cancer, Faculty of High Studies Zaragoza, National 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Implications","volume":"12","author":"Mojtahedi","year":"2021","journal-title":"Stem Cell Res. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e121","DOI":"10.1016\/S2352-3026(21)00370-7","article-title":"Ponatinib with Fludarabine, Cytarabine, Idarubicin, and Granulocyte Colony-Stimulating Factor Chemotherapy for Patients with Blast-Phase Chronic Myeloid Leukaemia (MATCHPOINT): A Single-Arm, Multicentre, Phase 1\/2 Trial","volume":"9","author":"Copland","year":"2022","journal-title":"Lancet Haematol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1038\/s41375-025-02664-w","article-title":"2025 European LeukemiaNet Recommendations for the Management of Chronic Myeloid Leukemia","volume":"39","author":"Apperley","year":"2025","journal-title":"Leukemia"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Eiring, A.M., Khorashad, J.S., Morley, K., and Deininger, M.W. (2011). Advances in the Treatment of Chronic Myeloid Leukemia. 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Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1158\/2643-3230.BCD-22-0098","article-title":"A Therapeutically Targetable NOTCH1\u2013SIRT1\u2013KAT7 Axis in T-Cell Leukemia","volume":"4","author":"Lancho","year":"2023","journal-title":"Blood Cancer Discov."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Romero-Trejo, D., Agui\u00f1iga-S\u00e1nchez, I., Velasco-Garc\u00eda, A., Rodr\u00edguez-Ter\u00e1n, K.M., Flores-Borja, F., Soto-Cruz, I., Legorreta-Herrera, M., Mac\u00edas-Zaragoza, V.M., Romero-L\u00f3pez, E., and Weiss-Steider, B. (2025). Sodium Caseinate Induces Apoptosis in Cytarabine-Resistant AML by Modulating SIRT1 and Chemoresistance Genes, Alone or in Combination with Cytarabine or Daunorubicin. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26157468"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Santiago-Osorio, E., Romero-Trejo, D., Mac\u00edas-Zaragoza, V.M., Rodr\u00edguez-Ter\u00e1n, K.M., Mendoza-N\u00fa\u00f1ez, V.M., Sierra-Mondrag\u00f3n, E., Romero-L\u00f3pez, E., Shira, L., Hern\u00e1ndez-\u00c1lvarez, D., and Agui\u00f1iga-S\u00e1nchez, I. (2025). Antileukemic Potential of Sodium Caseinate in Cytarabine-Resistant HL60-CR50 Human Leukemia Cells. Molecules, 30.","DOI":"10.3390\/molecules30244759"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Yang, Y., Liu, Y., Wang, Y., Chao, Y., Zhang, J., Jia, Y., Tie, J., and Hu, D. (2022). Regulation of SIRT1 and Its Roles in Inflammation. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.831168"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2012","DOI":"10.7150\/jca.24275","article-title":"SIRT1 Promotes Formation of Breast Cancer through Modulating Akt Activity","volume":"9","author":"Jin","year":"2018","journal-title":"J. Cancer"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"125","DOI":"10.3892\/or.2013.2470","article-title":"SIRT1 Suppresses Breast Cancer Growth through Downregulation of the Bcl-2 Protein","volume":"30","author":"Kuo","year":"2013","journal-title":"Oncol. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s10238-012-0186-5","article-title":"MiR-34a Inhibits Proliferation and Migration of Breast Cancer through down-Regulation of Bcl-2 and SIRT1","volume":"13","author":"Li","year":"2013","journal-title":"Clin. Exp. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/sj.onc.1209049","article-title":"Sirt1 Inhibitor, Sirtinol, Induces Senescence-like Growth Arrest with Attenuated Ras\u2013MAPK Signaling in Human Cancer Cells","volume":"25","author":"Ota","year":"2006","journal-title":"Oncogene"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1016\/j.bbapap.2010.05.002","article-title":"SIRT1 and P53, Effect on Cancer, Senescence and Beyond","volume":"1804","author":"Yi","year":"2010","journal-title":"Biochim. Biophys. Acta BBA-Proteins Proteom."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1530\/JOE-11-0217","article-title":"Sirtuin 1 (SIRT1) and Steroid Hormone Receptor Activity in Cancer","volume":"213","author":"Moore","year":"2012","journal-title":"J. Endocrinol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6726","DOI":"10.7150\/thno.100667","article-title":"Targeting Sirtuins for Cancer Therapy: Epigenetics Modifications and Beyond","volume":"14","author":"Shen","year":"2024","journal-title":"Theranostics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5289","DOI":"10.1074\/jbc.M110.163667","article-title":"FoxO1 Mediates an Autofeedback Loop Regulating SIRT1 Expression","volume":"286","author":"Xiong","year":"2011","journal-title":"J. Biol. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Mansouri, R., Heydarpour, F., Yari, K., Naseroleslami, M., and Payandeh, M. (2025). Study of the Association between HDAC8, SIRT1, and P53 Gene Expression with Drug Resistance in Chronic Myeloid Leukemia Patients. BMC Cancer, 25.","DOI":"10.1186\/s12885-025-15070-3"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"147917","DOI":"10.1016\/j.gene.2023.147917","article-title":"circ_SIRT1 Upregulates ATG12 to Facilitate Imatinib Resistance in CML through Interacting with EIF4A3","volume":"893","author":"Zhang","year":"2024","journal-title":"Gene"},{"key":"ref_21","first-page":"41","article-title":"Production, Genetics, Postharvest Management and Pharmacological Characteristics of Sechium edule (Jacq)","volume":"1","author":"Cisneros","year":"2007","journal-title":"Sw. Fresh Prod."},{"key":"ref_22","first-page":"27","article-title":"The Antihypertensive Effects of the Jamaican Cho-Cho (Sechium edule)","volume":"49","author":"Gordon","year":"2000","journal-title":"West Indian Med. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1080\/01635581.2015.989370","article-title":"Fruit Extract from A Sechium edule Hybrid Induce Apoptosis in Leukaemic Cell Lines but Not in Normal Cells","volume":"67","year":"2015","journal-title":"Nutr. Cancer"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Salazar-Aguilar, S., Ruiz-Posadas, L., Cadena-I\u00f1iguez, J., Soto-Hern\u00e1ndez, M., Santiago-Osorio, E., Agui\u00f1iga-S\u00e1nchez, I., Rivera-Mart\u00ednez, A., and Aguirre-Medina, J. (2017). Sechium edule (Jacq.) Swartz, a New Cultivar with Antiproliferative Potential in a Human Cervical Cancer HeLa Cell Line. Nutrients, 9.","DOI":"10.3390\/nu9080798"},{"key":"ref_25","first-page":"481","article-title":"Las Variedades Del Chayote (Sechium edule (Jacq.) Sw.) y Su Comercio Mundial","volume":"9","year":"2012","journal-title":"Agric. Soc. Desarro."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Delgado-Tiburcio, E.E., Soto-Hern\u00e1ndez, R.M., Agui\u00f1iga-S\u00e1nchez, I., Cadena-I\u00f1iguez, J., Ruiz-Posadas, L.D.M., Pe\u00f1a-Valdivia, C.B., and G\u00f3mez-Y\u00e1\u00f1ez, H. (2025). Pharmacokinetic Profile of Extracts from the Chayote (Sechium edule) H387 07 Hybrid and Phytochemical Characterization of Its Segregant H387 M16 for Potential Therapeutic Applications. Molecules, 30.","DOI":"10.3390\/molecules30193948"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Agui\u00f1iga-S\u00e1nchez, I., Soto-Hern\u00e1ndez, M., Cadena-I\u00f1iguez, J., Suwalsky, M., Colina, J.R., Castillo, I., Rosado-P\u00e9rez, J., Mendoza-N\u00fa\u00f1ez, V.M., and Santiago-Osorio, E. (2020). Phytochemical Analysis and Antioxidant and Anti-Inflammatory Capacity of the Extracts of Fruits of the Sechium Hybrid. Molecules, 25.","DOI":"10.3390\/molecules25204637"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1016\/0145-2126(93)90161-D","article-title":"Mutational Inactivation of the P53 Gene in the Human Erythroid Leukemic K562 Cell Line","volume":"17","author":"Law","year":"1993","journal-title":"Leuk. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.ccr.2011.12.020","article-title":"Activation of P53 by SIRT1 Inhibition Enhances Elimination of CML Leukemia Stem Cells in Combination with Imatinib","volume":"21","author":"Li","year":"2012","journal-title":"Cancer Cell"},{"key":"ref_30","first-page":"5233","article-title":"Recent Advances of SIRT1 and Implications in Chemotherapeutics Resistance in Cancer","volume":"11","author":"Yousafzai","year":"2021","journal-title":"Am. J. Cancer Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.7754\/Clin.Lab.2020.200835","article-title":"Sirtuin1 and Chronic Myeloid Leukemia: A Comprehensive Glance at Drug Resistance","volume":"67","author":"Abbasian","year":"2021","journal-title":"Clin. Lab."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2685","DOI":"10.1172\/JCI127080","article-title":"SIRT1 Regulates Metabolism and Leukemogenic Potential in CML Stem Cells","volume":"129","author":"Abraham","year":"2019","journal-title":"J. Clin. Investig."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1097\/MOH.0b013e328360ab64","article-title":"Roles of SIRT1 in Leukemogenesis","volume":"20","author":"Chen","year":"2013","journal-title":"Curr. Opin. Hematol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.canlet.2009.07.015","article-title":"Cucurbitacin B Inhibits STAT3 and the Raf\/MEK\/ERK Pathway in Leukemia Cell Line K562","volume":"289","author":"Chan","year":"2010","journal-title":"Cancer Lett."},{"key":"ref_35","first-page":"63","article-title":"Desarrollo y Transferencia Del H\u00edbrido Amargo de Chayote [Sechium edule (Jacq) Sw.] \u201cH 387 07\u201d","volume":"3","year":"2024","journal-title":"Agro-Divulgaci\u00f3n"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Heinrich, M., Jalil, B., Abdel-Tawab, M., Echeverria, J., Kuli\u0107, \u017d., McGaw, L.J., Pezzuto, J.M., Potterat, O., and Wang, J.-B. (2022). Best Practice in the Chemical Characterisation of Extracts Used in Pharmacological and Toxicological Research\u2014The ConPhyMP\u2014Guidelines12. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.953205"},{"key":"ref_37","unstructured":"Cadena I\u00f1iguez, J. (2005). Caracterizaci\u00f3n Morfoestructural, Fisiol\u00f3gica, Qu\u00edmica y Gen\u00e9tica de Diferentes Tipos de Chayote (Sechium edule (Jacq.)Sw). [Ph.D. Thesis, Colegio de Postgraduados]."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/17\/2998\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T08:18:19Z","timestamp":1789114699000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/17\/2998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,27]]},"references-count":38,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["molecules31172998"],"URL":"https:\/\/doi.org\/10.3390\/molecules31172998","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,27]]}}}