{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T05:15:45Z","timestamp":1788498945149,"version":"build-2803163510"},"reference-count":57,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM152280"],"award-info":[{"award-number":["P20GM152280"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM103638"],"award-info":[{"award-number":["P20GM103638"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P30GM145499"],"award-info":[{"award-number":["P30GM145499"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"award":["P20GM152280"],"award-info":[{"award-number":["P20GM152280"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"publisher"}]},{"award":["P20GM103638"],"award-info":[{"award-number":["P20GM103638"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"publisher"}]},{"award":["P30GM145499"],"award-info":[{"award-number":["P30GM145499"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000890","name":"National Multiple Sclerosis Society","doi-asserted-by":"publisher","award":["RFA-2312-42463"],"award-info":[{"award-number":["RFA-2312-42463"]}],"id":[{"id":"10.13039\/100000890","id-type":"DOI","asserted-by":"publisher"}]},{"award":["RFA-2312-42463"],"award-info":[{"award-number":["RFA-2312-42463"]}],"id":[{"id":"https:\/\/ror.org\/03019wv17","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Research Experiences for Undergraduates","award":["1950293"],"award-info":[{"award-number":["1950293"]}]},{"award":["1950293"],"award-info":[{"award-number":["1950293"]}],"id":[{"id":"https:\/\/ror.org\/020fhsn68","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Cholesterol in the central nervous system (CNS) is largely unesterified (&gt;99%) and is predominantly present in the myelin sheath (~70% of total CNS cholesterol). However, cholesterol ester accumulation has been observed during neurological disease. In this cross-sectional study, we measured free cholesterol and cholesterol ester levels in the brains and the spinal cords of Plp1-iCKO-Myrf mouse model during postnatal myelination, demyelination, and remyelination using gas chromatography\u2013mass spectrometry with single ion monitoring (GC-MS-SIM) and liquid chromatography\u2013mass spectrometry (LC-MS). Cholesterol levels in healthy mouse brains increased steadily up to 38 weeks of age. In contrast, cholesterol in the healthy spinal cord increased until P42, and then remained steady out to 38 weeks of age. During demyelination, both brain and spinal cord cholesterol levels were significantly reduced compared to healthy mice and did not return to normal cholesterol levels even during remyelination. Semi-quantitative quantification of cholesterol esters during peak demyelination revealed that cholesterol esters comprise 19% of the measured cholesterol pool in the brain and 66% in the spinal cord. Robust methods for internal standard-based quantification of CNS cholesterol and cholesterol esters were developed, which are critical for revealing mechanisms of cholesterol regulation during disease.<\/jats:p>","DOI":"10.3390\/molecules31173033","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:24:03Z","timestamp":1788150243000},"page":"3033","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cholesterol Quantification in Brain and Spinal Cord During Development, Demyelination, and Remyelination"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6312-7163","authenticated-orcid":false,"given":"Disni","family":"Dedunupitiya","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, KS 66045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eden P.","family":"Go","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, KS 66045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Travis","family":"Witte","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, KS 66045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashlynn","family":"Elliott","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, KS 66045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nishama De Silva","family":"Mohotti","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, KS 66045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenna M.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, KS 66045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, KS 66045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashmi","family":"Binjawadagi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, KS 66045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2181-0112","authenticated-orcid":false,"given":"Heather","family":"Desaire","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, KS 66045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6229-2895","authenticated-orcid":false,"given":"Meredith D.","family":"Hartley","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, KS 66045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1097\/00041433-200104000-00003","article-title":"Cholesterol metabolism in the brain","volume":"12","author":"Dietschy","year":"2001","journal-title":"Curr. Opin. Lipidol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1002\/glia.70042","article-title":"Myelin Lipid Composition in the Central Nervous System Is Regionally Distinct and Requires Mechanistic Target of Rapamycin Signaling","volume":"73","author":"Mather","year":"2025","journal-title":"Glia"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1161\/01.ATV.0000120374.59826.1b","article-title":"Brain cholesterol: Long secret life behind a barrier","volume":"24","author":"Meaney","year":"2004","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"29","DOI":"10.3109\/10408369309084665","article-title":"The structure and function of central nervous system myelin","volume":"30","author":"Williams","year":"1993","journal-title":"Crit. Rev. Clin. Lab. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Poitelon, Y., Kopec, A.M., and Belin, S. (2020). Myelin fat facts: An overview of lipids and fatty acid metabolism. Cells, 9.","DOI":"10.3390\/cells9040812"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3154","DOI":"10.1073\/pnas.0813110106","article-title":"Interaction forces and adhesion of supported myelin lipid bilayers modulated by myelin basic protein","volume":"106","author":"Min","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1038\/nn1426","article-title":"High cholesterol level is essential for myelin membrane growth","volume":"8","author":"Saher","year":"2005","journal-title":"Nat. Neurosci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1007\/s13238-014-0131-3","article-title":"Cholesterol metabolism and homeostasis in the brain","volume":"6","author":"Zhang","year":"2015","journal-title":"Protein Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1126\/science.147.3662.1099","article-title":"Stability of the Myelin Membrane: Lipid molecules may impart stability to the myelin membrane through intermolecular cohesion","volume":"147","year":"1965","journal-title":"Science"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"159637","DOI":"10.1016\/j.bbalip.2025.159637","article-title":"Reduced cholesterol alters the biophysical properties of repaired myelin","volume":"1870","author":"Escobar","year":"2025","journal-title":"Biochim. Biophys. Acta Mol. Cell Biol. Lipids"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1159\/000537794","article-title":"The effects of neuroactive steroids on myelin in health and disease","volume":"33","author":"Kalakh","year":"2024","journal-title":"Med. Princ. Pract."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"292598","DOI":"10.1155\/2012\/292598","article-title":"Cholesterol: Its regulation and role in central nervous system disorders","volume":"2012","author":"Orth","year":"2012","journal-title":"Cholesterol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.msard.2015.10.005","article-title":"Cholesterol and markers of cholesterol turnover in multiple sclerosis: Relationship with disease outcomes","volume":"5","author":"Zhornitsky","year":"2016","journal-title":"Mult. Scler. Relat. Disord."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"109889","DOI":"10.1016\/j.celrep.2021.109889","article-title":"Neuronal cholesterol synthesis is essential for repair of chronically demyelinated lesions in mice","volume":"37","author":"Berghoff","year":"2021","journal-title":"Cell Rep."},{"key":"ref_15","first-page":"340","article-title":"Cholesteryl esters in apparently normal white matter in multiple sclerosis","volume":"10","author":"Wender","year":"1973","journal-title":"Eur. Neurol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1136\/jnnp.22.2.117","article-title":"The cholesterol and cholesterol ester content of cerebrospinal fluid in patients with multiple sclerosis and other neurological diseases","volume":"22","author":"Green","year":"1959","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1111\/j.1471-4159.1982.tb03968.x","article-title":"Lipid and protein alterations of spinal cord and cord myelin of multiple sclerosis","volume":"39","author":"Yu","year":"1982","journal-title":"J. Neurochem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/j.neuron.2019.12.007","article-title":"TREM2 regulates microglial cholesterol metabolism upon chronic phagocytic challenge","volume":"105","author":"Nugent","year":"2020","journal-title":"Neuron"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0014-5793(78)80091-X","article-title":"Experimental allergic encephalomyelitis: Identification of the myelin component responsible for the appearance of brain esterified cholesterol","volume":"86","author":"Roth","year":"1978","journal-title":"FEBS Lett."},{"key":"ref_20","first-page":"160","article-title":"Temporal dynamics of CNS cholesterol esters correlate with demyelination and remyelination","volume":"17","author":"Williams","year":"2025","journal-title":"ACS Chem. Neurosci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Huynh, T.N., Havrda, M.C., Zanazzi, G.J., Chang, C.C., and Chang, T.Y. (2024). Inhibiting the cholesterol storage enzyme ACAT1\/SOAT1 in myelin debris-treated microglial cell lines activates the gene expression of cholesterol efflux transporter ABCA1. Biomolecules, 14.","DOI":"10.3390\/biom14101301"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1194\/jlr.M800584-JLR200","article-title":"LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins","volume":"50","author":"Donkin","year":"2009","journal-title":"J. Lipid Res."},{"key":"ref_23","first-page":"2830","article-title":"Lipidomic analysis reveals differences in the extent of remyelination in the brain and spinal cord","volume":"23","author":"Kobayashi","year":"2023","journal-title":"J. Proteome Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"579022","DOI":"10.1016\/j.jneuroim.2026.579022","article-title":"Spinal cord microglia exhibit a dysfunctional response to myelin damage","volume":"419","author":"Zupan","year":"2026","journal-title":"J. Neuroimmunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2778","DOI":"10.1016\/j.jchromb.2009.05.017","article-title":"Analysis of neurosterols by GC-MS and LC-MS\/MS","volume":"877","author":"Griffiths","year":"2009","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2130","DOI":"10.1016\/j.jchromb.2010.06.010","article-title":"Quantitative analysis of five sterols in amniotic fluid by GC\u2013MS: Application to the diagnosis of cholesterol biosynthesis defects","volume":"878","author":"Amaral","year":"2010","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_27","unstructured":"Zaikin, V., and Halket, J.M. (2009). A Handbook of Derivatives for Mass Spectrometry, IM Publications."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.bbalip.2005.12.007","article-title":"High throughput quantification of cholesterol and cholesteryl ester by electrospray ionization tandem mass spectrometry (ESI-MS\/MS)","volume":"1761","author":"Liebisch","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.cell.2009.04.031","article-title":"Myelin gene regulatory factor is a critical transcriptional regulator required for CNS myelination","volume":"138","author":"Emery","year":"2009","journal-title":"Cell"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"12528","DOI":"10.1523\/JNEUROSCI.1069-12.2012","article-title":"Myelin gene regulatory factor is required for maintenance of myelin and mature oligodendrocyte identity in the adult CNS","volume":"32","author":"Koenning","year":"2012","journal-title":"J. Neurosci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e126329","DOI":"10.1172\/jci.insight.126329","article-title":"Myelin repair stimulated by CNS-selective thyroid hormone action","volume":"4","author":"Hartley","year":"2019","journal-title":"JCI Insight"},{"key":"ref_32","unstructured":"U.S. Food and Drug Administration (2018). Bioanalytical Method Validation\u2014Guidance for Industry."},{"key":"ref_33","unstructured":"Fox, J.G., Barthold, S.W., Davisson, M.T., Newcomer, C.E., Quimby, F.W., and Smith, A.L. (2007). Chapter 20\u2014Mouse models in aging research. The Mouse in Biomedical Research, Elsevier."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2743","DOI":"10.1016\/j.bmc.2017.03.047","article-title":"Ester-to-amide rearrangement of ethanolamine-derived prodrugs of sobetirome with increased blood-brain barrier penetration","volume":"25","author":"Ferrara","year":"2017","journal-title":"Bioorg. Med. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2468","DOI":"10.1021\/acschemneuro.7b00239","article-title":"Targeting fatty-acid amide hydrolase with prodrugs for CNS-selective therapy","volume":"8","author":"Meinig","year":"2017","journal-title":"ACS Chem. Neurosci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"15490","DOI":"10.1073\/pnas.0702759104","article-title":"Targeting thyroid hormone receptor-\u03b2 agonists to the liver reduces cholesterol and triglycerides and improves the therapeutic index","volume":"104","author":"Erion","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.tins.2022.01.001","article-title":"Local cholesterol metabolism orchestrates remyelination","volume":"45","author":"Berghoff","year":"2022","journal-title":"Trends Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"577468","DOI":"10.1016\/j.jneuroim.2020.577468","article-title":"Thyroid hormone and thyromimetics inhibit myelin and axonal degeneration and oligodendrocyte loss in EAE","volume":"352","author":"Chaudhary","year":"2021","journal-title":"J. Neuroimmunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1194\/jlr.R400004-JLR200","article-title":"Thematic review series: Brain Lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal","volume":"45","author":"Dietschy","year":"2004","journal-title":"J. Lipid Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Diotel, N., Charlier, T.D., Lefebvre d\u2019Hellencourt, C., Couret, D., Trudeau, V.L., Nicolau, J.C., Meilhac, O., Kah, O., and Pellegrini, E. (2018). Steroid transport, local synthesis, and signaling within the brain: Roles in neurogenesis, neuroprotection, and sexual behaviors. Front. Neurosci., 12.","DOI":"10.3389\/fnins.2018.00084"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"14352","DOI":"10.1016\/S0021-9258(18)47945-8","article-title":"Lipoproteins and their receptors in the central nervous system. Characterization of the lipoproteins in cerebrospinal fluid and identification of apolipoprotein B, E (LDL) receptors in the brain","volume":"262","author":"Pitas","year":"1987","journal-title":"J. Biol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1016\/S0022-2275(20)32188-X","article-title":"Cholesterol homeostasis in human brain: Turnover of 24S-hydroxycholesterol and evidence for a cerebral origin of most of this oxysterol in the circulation","volume":"39","author":"Diczfalusy","year":"1998","journal-title":"J. Lipid Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3169","DOI":"10.1021\/bi00862a026","article-title":"A comprehensive study of the postnatal changes in the concentration of the lipids of developing rat brain","volume":"6","author":"Wells","year":"1967","journal-title":"Biochemistry"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.devbrainres.2003.09.015","article-title":"Ontogenesis and regulation of cholesterol metabolism in the central nervous system of the mouse","volume":"146","author":"Quan","year":"2003","journal-title":"Brain Res. Dev. Brain Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.2174\/1570159X19666211111122311","article-title":"Cholesterol in brain development and perinatal brain injury: More than a building block","volume":"20","author":"Lu","year":"2022","journal-title":"Curr. Neuropharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2680","DOI":"10.1172\/JCI119813","article-title":"Fatty streak formation occurs in human fetal aortas and is greatly enhanced by maternal hypercholesterolemia. Intimal accumulation of low density lipoprotein and its oxidation precede monocyte recruitment into early atherosclerotic lesions","volume":"100","author":"Napoli","year":"1997","journal-title":"J. Clin. Investig."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/S0022-2275(20)39618-8","article-title":"Developing rat brain: Changes in cholesterol, galactolipids, and the individual fatty acids of gangliosides and glycerophosphatides","volume":"6","author":"Kishimoto","year":"1965","journal-title":"J. Lipid Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1111\/j.1365-2990.1980.tb00219.x","article-title":"Myelination of the mouse corpus callosum","volume":"6","author":"Sturrock","year":"1980","journal-title":"Neuropathol. Appl. Neurobiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1038\/s41593-018-0121-5","article-title":"Myelin remodeling through experience-dependent oligodendrogenesis in the adult somatosensory cortex","volume":"21","author":"Hughes","year":"2018","journal-title":"Nat. Neurosci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1038\/s41593-018-0120-6","article-title":"Lifelong cortical myelin plasticity and age-related degeneration in the live mammalian brain","volume":"21","author":"Hill","year":"2018","journal-title":"Nat. Neurosci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"110423","DOI":"10.1016\/j.celrep.2022.110423","article-title":"Cholesterol biosynthesis defines oligodendrocyte precursor heterogeneity between brain and spinal cord","volume":"38","author":"Khandker","year":"2022","journal-title":"Cell Rep."},{"key":"ref_52","unstructured":"Siegel, G.J. (1999). Myelin formation, structure and biochemistry. Basic Neurochemistry, Lippincott-Raven. [6th ed.]."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"188-e4","DOI":"10.1111\/ene.14055","article-title":"Cholesterol and neurodegeneration: Longitudinal changes in serum cholesterol biomarkers are associated with new lesions and gray matter atrophy in multiple sclerosis over 5 years of follow-up","volume":"27","author":"Murali","year":"2020","journal-title":"Eur. J. Neurol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"103088","DOI":"10.1016\/j.autrev.2022.103088","article-title":"The role of cholesterol metabolism in multiple sclerosis: From molecular pathophysiology to radiological and clinical disease activity","volume":"21","author":"Lorincz","year":"2022","journal-title":"Autoimmun. Rev."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1002\/gene.10154","article-title":"Inducible site-specific recombination in myelinating cells","volume":"35","author":"Doerflinger","year":"2003","journal-title":"Genesis"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"9148","DOI":"10.1038\/s41467-024-53429-5","article-title":"Remyelination protects neurons from DLK-mediated neurodegeneration","volume":"15","author":"Duncan","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/17\/3033\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T04:25:35Z","timestamp":1788495935000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/17\/3033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,28]]},"references-count":57,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["molecules31173033"],"URL":"https:\/\/doi.org\/10.3390\/molecules31173033","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,28]]}}}