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The extracts were prepared using 90% v\/v ethanol and the phytochemical contents were assessed using spectrophotometric assays, while independent metabolites were identified and quantified using an LC\/MS\/ESI method. The antioxidant potential was evaluated by the DPPH radical and FRAP scavenging assays. Cytotoxicity was evaluated using human LX-2 hepatic stellate cells and HepG2 hepatocellular carcinoma cells. Cell viability assays were performed to determine the potential cytotoxic effects of the two extracts on cellular viability and overall metabolic activity. In vivo, hepatoprotective potential was assessed in a CCl4-induced model of hepatotoxicity. Results revealed the presence of flavonoids and polyphenolic compounds in the leaves of both species, with a more significant potential for the B. pendula leaf extracts. The cell viability assays showed that both extracts maintained high cell viability in LX-2 cells, whereas B. pubescens induced a more pronounced reduction in HepG2 cell viability than B. pendula under the tested experimental conditions. In vivo, CCl4 increased ALT activity to 193.0 \u00b1 8.5 U\/L, whereas B. pendula and B. pubescens at 200 mg\/kg reduced ALT to 30.4 \u00b1 6.6 and 117.4 \u00b1 24.3 U\/L, respectively. CCl4 also increased hepatic MDA levels from 202.7 \u00b1 31.8 to 717.1 \u00b1 31.6, which were reduced to 430.7 \u00b1 100.5 and 448.7 \u00b1 65.8 following treatment with B. pendula and B. pubescens at 200 mg\/kg, respectively.<\/jats:p>","DOI":"10.3390\/molecules31173037","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:24:03Z","timestamp":1788150243000},"page":"3037","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Metabolite Profiling of Betula pendula Roth and Betula pubescens Ehrh. 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