{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T05:17:25Z","timestamp":1788239845658,"version":"build-2803163510"},"reference-count":55,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education","doi-asserted-by":"crossref","award":["N310 305239"],"award-info":[{"award-number":["N310 305239"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"crossref"}]},{"award":["N310 305239"],"award-info":[{"award-number":["N310 305239"]}],"id":[{"id":"https:\/\/ror.org\/05dwvd537","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Biodegradable alginate films containing silver nanoparticles (AgNPs) are being increasingly investigated as active agricultural materials (e.g., antimicrobial mulches) and active food packaging. However, since these materials ultimately degrade in soil after use, assessing their environmental compatibility and potential phytotoxicity upon release is crucial. The aim of this study was to synthesize films containing AgNPs in sodium alginate using xylose as a reducing agent and to determine their effect on culturable rhizosphere microorganisms and selected biochemical parameters in young arugula (Eruca vesicaria L. subsp. sativa) plants. Alginate films containing three nominal AgNP loadings (50, 100, and 150 mg L\u22121) and a control film without AgNPs were synthesized. The films were cut into square pieces (4 cm2) and placed in 0.076 L multipots filled with peat substrate, into which arugula seeds were sown. During the experiment, the abundance of culturable rhizosphere bacteria and fungi was determined, and the young arugula plants were subjected to biochemical analyses. The results showed that the AgNP-containing films did not significantly affect the abundance of bacteria and fungi in the rhizosphere under the conditions tested. The tested films also did not markedly alter the measured parameters in the tissues of young arugula plants, including ascorbic acid, photosynthetic pigments, sugars, dietary protein, and glutathione. However, they reduced phenolic content, altered antioxidant activity, and led to detectable silver accumulation in plant tissues, especially at the highest nominal AgNP loading (150 mg L\u22121). These findings indicate limited but selective biochemical effects during the early growth stage of arugula rather than a complete absence of plant response.<\/jats:p>","DOI":"10.3390\/molecules31173045","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:24:03Z","timestamp":1788150243000},"page":"3045","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Effect of Alginate-Based Silver Nanoparticle Films on Young Arugula Plants (Eruca vesicaria L. subsp. sativa)"],"prefix":"10.3390","volume":"31","author":[{"given":"Mi\u0142osz","family":"Rutkowski","sequence":"first","affiliation":[{"name":"Centre for Innovation and Research on Prohealthy and Safe Food, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland"},{"name":"Faculty of Food Technology, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland"},{"name":"Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4601-7145","authenticated-orcid":false,"given":"Gohar","family":"Khachatryan","sequence":"additional","affiliation":[{"name":"Faculty of Food Technology, University of Agriculture in Krakow, Al. 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Mickiewicza 21, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Lidia","family":"Krzemi\u0144ska-Fiedorowicz","sequence":"additional","affiliation":[{"name":"Faculty of Food Technology, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8437-9307","authenticated-orcid":false,"given":"Andrzej","family":"Kalisz","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Joanna","family":"Gil","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Adam","family":"Florkiewicz","sequence":"additional","affiliation":[{"name":"Faculty of Food Technology, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0254-5492","authenticated-orcid":false,"given":"Katarzyna","family":"Starzec","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8507-8928","authenticated-orcid":false,"given":"Przemys\u0142aw","family":"Petryszak","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4968-8833","authenticated-orcid":false,"given":"Pawe\u0142","family":"Kaszycki","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9655-5742","authenticated-orcid":false,"given":"Agnieszka","family":"S\u0119kara","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 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Nanomaterials, 16.","DOI":"10.3390\/nano16020134"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Salam, A., Khan, A.R., Zeeshan, M., Afridi, M.S., Yang, L., Zheng, Q., Ulhassan, Z., Wang, R., Zhang, Z., and Zhao, C. (2026). Nano-Enabled Solutions for Plant Abiotic Stress Tolerance and Soil Contaminant Remediation: A Review. Plants, 15.","DOI":"10.3390\/plants15040535"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Karata\u015f, H., Eker, F., Akda\u015f\u00e7i, E., Bechelany, M., and Karav, S. (2026). Silver Nanoparticles in Antibacterial Research: Mechanisms, Applications, and Emerging Perspectives. Int. J. Mol. Sci., 27.","DOI":"10.3390\/ijms27020927"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Guzm\u00e1n-B\u00e1ez, G.A., Trejo-T\u00e9llez, L.I., Navarro-L\u00f3pez, D.E., Mej\u00eda-M\u00e9ndez, J.L., and G\u00f3mez-Merino, F.C. (2026). Influence of Silver Nanoparticles (AgNPs) on Vegetative Growth and Concentrations of Nutrients and Phytohormones in Tomato. Plants, 15.","DOI":"10.3390\/plants15030405"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Rutkowski, M., Krzemi\u0144ska-Fiedorowicz, L., Khachatryan, G., Kabaci\u0144ska, J., Tischner, M., Suder, A., Kulik, K., and Lenart-Boro\u0144, A. (2022). Antibacterial Properties of Biodegradable Silver Nanoparticle Foils Based on Various Strains of Pathogenic Bacteria Isolated from the Oral Cavity of Cats, Dogs and Horses. Materials, 15.","DOI":"10.3390\/ma15031269"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Mihailovi\u0107, V., Sre\u0107kovi\u0107, N., Nedi\u0107, Z.P., Dimitrijevi\u0107, S., Mati\u0107, M., Obradovi\u0107, A., Selakovi\u0107, D., Rosi\u0107, G., and Katani\u0107 Stankovi\u0107, J.S. (2023). Green Synthesis of Silver Nanoparticles Using Salvia verticillata and Filipendula ulmaria Extracts: Optimization of Synthesis, Biological Activities, and Catalytic Properties. Molecules, 28.","DOI":"10.3390\/molecules28020808"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Paramasivam, D., Balasubramanian, B., Suresh, R., Kumaravelu, J., Vellingiri, M.M., Liu, W.-C., Meyyazhagan, A., Alanazi, A.M., Rengasamy, K.R.R., and Arumugam, V.A. (2023). One-Pot Synthesis of Silver Nanoparticles Derived from Aqueous Leaf Extract of Ageratum conyzoides and Their Biological Efficacy. Antibiotics, 12.","DOI":"10.3390\/antibiotics12040688"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Zhang, J., Yin, Y., Hu, S., Wang, G., Tong, Y., Zen, M., Liang, Z., Li, M., Yan, R., and Wang, Y. (2023). Green Synthesis of Anti-bacterial Nano Silver by Polysaccharide from Bletilla Striata. Inorganics, 11.","DOI":"10.3390\/inorganics11010040"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Lee, S.H., and Jun, B.-H. (2019). Silver Nanoparticles: Synthesis and Application for Nanomedicine. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20040865"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"100491","DOI":"10.1016\/j.stress.2024.100491","article-title":"Impact of silver nanoparticles in alginate gels on seed germination, growth and stress biochemical parameters of cucumber seedlings","volume":"12","author":"Rutkowski","year":"2024","journal-title":"Plant Stress"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Szopa, D., Wr\u00f3bel, P., Zwoli\u0144ska, J., Anwar, H., Kaniewski, M., and Witek-Krowiak, A. (2026). Influence of Deep Eutectic Solvents and Polyphenolic Extracts on the Structure and Functional Properties of Sodium Alginate Films. Polymers, 18.","DOI":"10.3390\/polym18020186"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s43088-021-00139-2","article-title":"Silver nanoparticles and Chlorella treatments induced glucosinolates and kaempferol key biosynthetic genes in Eruca sativa","volume":"10","author":"Elsherif","year":"2021","journal-title":"Beni-Suef Univ. J. Basic. Appl. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"50","DOI":"10.46793\/ChemN3.1.050A","article-title":"Eco-friendly polymer succinate capping on silver nano-particles for enhanced stability: A UV-Vis and electrochemical particle impact study","volume":"3","author":"Abbas","year":"2020","journal-title":"Chem. Naissensis"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"107981","DOI":"10.1016\/j.jwpe.2025.107981","article-title":"Dual colorimetric sensing of Hg(II) and Fe(III) using sulfanilamide-stabilized silver nanoparticles and evaluating their photodegradation and antibacterial properties","volume":"75","author":"Kanwal","year":"2025","journal-title":"J. Water Process Eng."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Vannini, C., Domingo, G., Onelli, E., Prinsi, B., Marsoni, M., Espen, L., and Bracale, M. (2013). Morphological and proteomic responses of Eruca sativa exposed to silver nanoparticles or silver nitrate. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0068752"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6791","DOI":"10.1007\/s00289-025-05802-7","article-title":"Improvement of antibacterial and mechanical properties of chitosan matrix biodegradable polymer coating materials with green synthesized nanosilver obtained from aromatic arugula (Eruca vesicaria)","volume":"82","author":"Erkan","year":"2025","journal-title":"Polym. Bull."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1126\/science.1114397","article-title":"Toxic potential of materials at the nanolevel","volume":"311","author":"Nel","year":"2006","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1007\/s10646-012-0862-y","article-title":"Practical considerations for conducting ecotoxicity test methods with manufactured nanomaterials: What have we learnt so far?","volume":"21","author":"Handy","year":"2012","journal-title":"Ecotoxicology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e202501882","DOI":"10.1002\/cssc.202501882","article-title":"Harnessing Sustainable Biopolymers: Engineered Alginate-Based Materials for Whole-Cell Environmental Remediation","volume":"19","author":"Gupta","year":"2026","journal-title":"ChemSusChem"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.soilbio.2015.08.019","article-title":"Effects of silver nanoparticles on soil microorganisms and maize biomass are linked in the rhizosphere","volume":"91","author":"Sillen","year":"2015","journal-title":"Soil Biol. Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1007\/s11051-013-1896-7","article-title":"Silver nanoparticles in soil\u2013plant systems","volume":"15","author":"Anjum","year":"2013","journal-title":"J. Nanopart. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/s11051-020-04866-y","article-title":"Silver nanoparticle detection and accumulation in tomato (Lycopersicon esculentum)","volume":"22","author":"Noori","year":"2020","journal-title":"J. Nanopart. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1002\/jps.2600521210","article-title":"Mechanism of sustained-action medication. Theoretical analysis of rate of release of solid drugs dispersed in solid matrices","volume":"52","author":"Higuchi","year":"1963","journal-title":"J. Pharm. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0378-5173(83)90064-9","article-title":"Mechanisms of solute release from porous hydrophilic polymers","volume":"15","author":"Korsmeyer","year":"1983","journal-title":"Int. J. Pharm."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ijpharm.2005.10.044","article-title":"On the use of the Weibull function for the discernment of drug release mechanisms","volume":"309","author":"Papadopoulou","year":"2006","journal-title":"Int. J. Pharm."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"OECD (2020). Guidance Document for the Testing of Dissolution and Dispersion Stability of Nanomaterials, and the Use of the Data for Further Environmental Testing and Assessment, OECD Publishing. OECD Series on Testing and Assessment, No. 318.","DOI":"10.1787\/f0539ec5-en"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Loveck\u00e1, P., Mac\u016frkov\u00e1, A., Z\u00e1ruba, K., Hub\u00e1\u010dek, T., Siegel, J., and Valentov\u00e1, O. (2021). Genomic Damage Induced in Nicotiana tabacum L. Plants by Colloidal Solution with Silver and Gold Nanoparticles. Plants, 10.","DOI":"10.3390\/plants10061260"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Jurkow, R., Pokluda, R., S\u0119kara, A., and Kalisz, A. (2020). Impact of foliar application of some metal nanoparticles on antioxidant system in oakleaf lettuce seedlings. BMC Plant Biol., 20.","DOI":"10.1186\/s12870-020-02490-5"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1016\/j.lwt.2007.06.019","article-title":"Physical, chemical and microbiological changes in stored green asparagus spears as affected by coating of silver nanoparticles-PVP","volume":"41","author":"An","year":"2008","journal-title":"LWT Food Sci. Technol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1007\/s13580-016-0083-z","article-title":"Silver nanoparticles and silver ions: Oxidative stress responses and toxicity in potato (Solanum tuberosum L.) grown in vitro","volume":"57","author":"Homaee","year":"2016","journal-title":"Hortic. Environ. Biotechnol."},{"key":"ref_31","first-page":"263","article-title":"Effect of silver nanoparticles and magnetic field on the yield and chemical composition of Triticum aestivum L. seedlings","volume":"3","author":"Najafi","year":"2014","journal-title":"Bull. Environ. Pharmacol. Life Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Alhammad, B.A., Abdel-Aziz, H.M.M., Seleiman, M.F., and Tourky, S.M.N. (2023). How Can Biological and Chemical Silver Nanoparticles Positively Impact Physio-Chemical and Chloroplast Ultrastructural Characteristics of Vicia faba Seedlings?. Plants, 12.","DOI":"10.3390\/plants12132509"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ecoenv.2012.10.018","article-title":"Effect of silver nanoparticles on Oryza sativa L. and its rhizosphere bacteria","volume":"88","author":"Mirzajani","year":"2013","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Hasan, M., Mehmood, K., Mustafa, G., Zafar, A., Tariq, T., Hassan, S.G., Loomba, S., Zia, M., Mazher, A., and Mahmood, N. (2021). Phytotoxic Evaluation of Phytosynthesized Silver Nanoparticles on Lettuce. Coatings, 11.","DOI":"10.3390\/coatings11020225"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Tomaszewska-Sowa, M., Lisiecki, K., and Pa\u0144ka, D. (2022). Response of Rapeseed (Brassica napus L.) to Silver and Gold Nanoparticles as a Function of Concentration and Length of Exposure. Agronomy, 12.","DOI":"10.3390\/agronomy12112885"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Aly, A.A., Eliwa, N.E., and Safwat, G. (2024). Role of gamma-irradiated sodium alginate on growth, physiological and active components of iceberg lettuce (Lactuca sativa) plant. BMC Plant Biol., 24.","DOI":"10.1186\/s12870-024-04853-8"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Zhou, Y., Wang, B., and Yuan, F. (2022). The Role of Transmembrane Proteins in Plant Growth, Development, and Stress Responses. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232113627"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s42269-019-0077-y","article-title":"Impact of silver nanoparticles on plant growth, some biochemical aspects, and yield of fenugreek plant (Trigonella foenum-graecum)","volume":"43","author":"Sadak","year":"2019","journal-title":"Bull. Natl. Res. Cent."},{"key":"ref_39","first-page":"190","article-title":"Effects of silver nanoparticles in some crop plants, common bean (Phaseolus vulgaris L.) and corn (Zea mays L.)","volume":"3","author":"Salama","year":"2012","journal-title":"Int. Res. J. Biotech."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Fu, J., Zheng, Y., Gao, Y., and Xu, W. (2022). Dietary Fiber Intake and Gut Microbiota in Human Health. Microorganisms, 10.","DOI":"10.3390\/microorganisms10122507"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Jeandet, P., Formela-Luboi\u0144ska, M., Labudda, M., and Morkunas, I. (2022). The Role of Sugars in Plant Responses to Stress and Their Regulatory Function during Development. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23095161"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Angelini, J., Klassen, R., \u0160irok\u00e1, J., Nov\u00e1k, O., Z\u00e1ruba, K., Siegel, J., Novotn\u00e1, Z., and Valentov\u00e1, O. (2022). Silver Nanoparticles Alter Microtubule Arrangement, Dynamics and Stress Phytohormone Levels. Plants, 11.","DOI":"10.3390\/plants11030313"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Sta\u0142anowska, K., Szabli\u0144ska-Piernik, J., Okorski, A., and Lahuta, L.B. (2023). Zinc Oxide Nanoparticles Affect Early Seedlings\u2019 Growth and Polar Metabolite Profiles of Pea (Pisum sativum L.) and Wheat (Triticum aestivum L.). Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241914992"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"141954","DOI":"10.1016\/j.chemosphere.2024.141954","article-title":"Enhancing nitrogen removal performance using immobilized aerobic denitrifying bacteria by modified polyvinyl alcohol\/sodium alginate (PVA\/SA)","volume":"357","author":"Jin","year":"2024","journal-title":"Chemosphere"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Rutkowski, M., Krzemi\u0144ska-Fiedorowicz, L., Khachatryan, G., Bulski, K., Ko\u0142ton, A., and Khachatryan, K. (2022). Biodegradable Silver Nanoparticles Gel and Its Impact on Tomato Seed Germination Rate in In Vitro Cultures. Appl. Sci., 12.","DOI":"10.3390\/app12052722"},{"key":"ref_46","first-page":"14","article-title":"Bacterial population dynamics in waste oily emulsions from the metal-processing industry","volume":"15","author":"Kaszycki","year":"2014","journal-title":"J. Ecol. Eng."},{"key":"ref_47","unstructured":"(2004). Foodstuffs-Determination of Trace Elements-Determination of Lead, Cadmium, Zinc, Copper and Iron by Atomic Absorption Spectrometry (AAS) after Microwave Digestion (Standard No. PN-EN 14084:2004)."},{"key":"ref_48","first-page":"47","article-title":"Iodometric determination of the ascorbic acid (vitamin C) content of some fruits consumed in a university community in Nigeria","volume":"17","author":"Ikewuchi","year":"2011","journal-title":"Glob. J. Pure Appl. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"733","DOI":"10.4141\/cjps83-090","article-title":"Variation in glutathione and ascorbic acid content among selected cultivars of Phaseolus vulgaris prior to and after exposure to ozone","volume":"63","author":"Guri","year":"1983","journal-title":"Can. J. Plant Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.foodchem.2005.04.028","article-title":"Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compound","volume":"97","author":"Djeridane","year":"2006","journal-title":"Food Chem."},{"key":"ref_51","first-page":"211","article-title":"The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity","volume":"26","author":"Molyneux","year":"2004","journal-title":"Songklanakarin J. Sci. Technol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1042\/bst0110591","article-title":"Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents","volume":"11","author":"Lichtenthaler","year":"1983","journal-title":"Biochem. Soc. Trans."},{"key":"ref_53","unstructured":"(2008). Food Products\u2014Determination of the Total Nitrogen Content by Combustion According to the Dumas Principle and Calculation of the Crude Protein Content. Part 1: Oilseeds and Animal Feeding Stuffs (Standard No. PN-EN ISO 16634-1:2008)."},{"key":"ref_54","unstructured":"Horwitz, W. (2003). Total dietary fiber in foods, enzymatic-gravimetric method. Official Methods of Analysis of AOAC International, AOAC International."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1042\/bj0570508","article-title":"The estimation of carbohydrates in plant extracts by anthrone","volume":"54","author":"Yemm","year":"1954","journal-title":"Biochem. J."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/17\/3045\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:33:27Z","timestamp":1788237207000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/17\/3045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,30]]},"references-count":55,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["molecules31173045"],"URL":"https:\/\/doi.org\/10.3390\/molecules31173045","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,30]]}}}