{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:10:44Z","timestamp":1788779444929,"version":"build-2803163510"},"reference-count":31,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science, Research and Innovation Fund","award":["B13F680066"],"award-info":[{"award-number":["B13F680066"]}]},{"name":"University of Phayao and the Thailand Science Research and Innovation Fund","award":["2368\/2568"],"award-info":[{"award-number":["2368\/2568"]}]},{"name":"Frontier Research and Innovation Cluster Fund, Naresuan University, Thailand","award":["R2569C006"],"award-info":[{"award-number":["R2569C006"]}]},{"award":["R2569C006"],"award-info":[{"award-number":["R2569C006"]}],"id":[{"id":"https:\/\/ror.org\/03e2qe334","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Center of Excellence for Innovation in Chemistry (PERCH-CIC), Office of the Ministry of Higher Education, Science, Research and Innovation, Thailand"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Artocarpin is a promising bioactive compound with anti-inflammatory potential; however, its limited aqueous solubility may restrict its direct biological application. Therefore, artocarpin-loaded chitosan microparticles (CSPs\/AE) were prepared as a biocompatible carrier to improve artocarpin delivery. This study aimed to evaluate the cytocompatibility, antioxidant activity, and anti-inflammatory effects of CSPs\/AE in lipopolysaccharide (LPS)-stimulated chondrocytes. CSPs\/AE maintained high cell viability (&gt;80% at 0.125 and 0.5 mg\/mL) and showed significantly lower cytotoxicity than free artocarpin. Morphological and DAPI analyses confirmed preserved cellular and nuclear integrity without marked apoptosis. CSPs\/AE at 0.25 mg\/mL reduced LPS-induced intracellular ROS accumulation by approximately 79% relative to the LPS group. Western blot results demonstrated inhibition of LPS-induced NF-\u03baB phosphorylation, with CSPs\/AE at 0.5 mg\/mL reducing p-NF-\u03baB expression to approximately 0.66-fold of that observed in the LPS group. CSPs\/AE also partially restored aggrecan and collagen II expression without promoting a fibrocartilage, as indicated by unchanged collagen I. These findings indicate that CSPs\/AE attenuate oxidative stress and inflammatory signaling while preserving cartilage matrix components, highlighting their potential for cartilage-protective applications.<\/jats:p>","DOI":"10.3390\/molecules31173129","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T10:30:46Z","timestamp":1788777046000},"page":"3129","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti-Inflammatory Effects of Artocarpin-Loaded Chitosan Microparticles on Inflammatory Chondrocytes"],"prefix":"10.3390","volume":"31","author":[{"given":"Piyapan","family":"Manklinniam","sequence":"first","affiliation":[{"name":"Division of Microbiology and Parasitology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8082-1623","authenticated-orcid":false,"given":"Ratsada","family":"Praphasawat","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, University of Phayao, Phayao 56000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Arthid","family":"Thim-Uam","sequence":"additional","affiliation":[{"name":"Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grissana","family":"Pook-In","sequence":"additional","affiliation":[{"name":"Division of Microbiology and Parasitology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Jarupa","family":"Viyoch","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9869-0529","authenticated-orcid":false,"given":"Atchariya","family":"Yosboonruang","sequence":"additional","affiliation":[{"name":"Division of Microbiology and Parasitology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.2147\/DDDT.S117432","article-title":"The Spice for Joint Inflammation: Anti-Inflammatory Role of Curcumin in Treating Osteoarthritis","volume":"10","author":"Chin","year":"2016","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Molnar, V., Mati\u0161i\u0107, V., Kodvanj, I., Bjelica, R., Jele\u010d, \u017d., Hudetz, D., Rod, E., \u010cukelj, F., Vrdoljak, T., and Vidovi\u0107, D. (2021). Cytokines and chemokines involved in osteoarthritis pathogenesis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22179208"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Chiang, Y.-F., Huang, K.-C., Wang, K.-L., Huang, Y.-J., Chen, H.-Y., Ali, M., Shieh, T.-M., and Hsia, S.-M. (2024). Protective Effects of an Oligo-Fucoidan-Based Formula against Osteoarthritis Development via iNOS and COX-2 Suppression following Monosodium Iodoacetate Injection. Mar. Drugs, 22.","DOI":"10.3390\/md22050211"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Chen, P., Ruan, A., Zhou, J., Huang, L., Zhang, X., Ma, Y., and Wang, Q. (2020). Cinnamic Aldehyde Inhibits Lipopolysaccharide-Induced Chondrocyte Inflammation and Reduces Cartilage Degeneration by Blocking the Nuclear Factor-Kappa B Signaling Pathway. Front. Pharmacol., 11.","DOI":"10.3389\/fphar.2020.00949"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"808","DOI":"10.3892\/etm.2021.10240","article-title":"Blocking TNF\u03b1 attenuates progressive cartilage matrix degradation in inflammatory arthritis","volume":"22","author":"Park","year":"2021","journal-title":"Exp. Ther. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"146","DOI":"10.11138\/ccmbm\/2017.14.1.146","article-title":"Effectiveness of Intra-Articular Injections of Hyalubrix Combined with Exercise Therapy in the Treatment of Hip Osteoarthritis","volume":"14","author":"Mauro","year":"2017","journal-title":"Clin. Cases Miner. Bone Metab."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"229","DOI":"10.7150\/ijbs.28830","article-title":"Psoralen Protects Chondrocytes, Exhibits Anti-Inflammatory Effects on Synoviocytes, and Attenuates Monosodium Iodoacetate-Induced Osteoarthritis","volume":"15","author":"Wang","year":"2019","journal-title":"Int. J. Biol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Maneechan, W., Khumfu, P., Charoensit, P., Tuanchai, A., Ross, S., Ross, G.M., and Viyoch, J. (2025). Bioactive Hydrogel Scaffolds Integrating Chitosan, Silk Fibroin, and Aloe vera Extract for Enhanced Cartilage Tissue Regeneration. Polymers, 17.","DOI":"10.3390\/polym17101409"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.fct.2013.07.029","article-title":"Artocarpin attenuates ultraviolet B-induced skin damage in hairless mice by antioxidant and anti-inflammatory effect","volume":"60","author":"Lee","year":"2013","journal-title":"Food Chem. Toxicol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"15599","DOI":"10.1038\/s41598-017-15876-7","article-title":"The Effects of Artocarpin on Wound Healing: In Vitro and In Vivo Studies","volume":"7","author":"Yeh","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6854","DOI":"10.1038\/s41598-021-86040-5","article-title":"Potential Chemopreventive, Anticancer and Anti-Inflammatory Properties of an Artocarpin-Rich Extract","volume":"11","author":"Morrison","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5067957","DOI":"10.1155\/2021\/5067957","article-title":"Prevention by Natural Artocarpin of Morphological and Biochemical Alterations on UVB-Induced HaCaT Cells","volume":"2021","author":"Luangpraditkun","year":"2021","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Manklinniam, P., Reuk-Ngam, P., Boontha, S., Luangpraditkun, K., Ross, S., Viyoch, J., and Yosboonruang, A. (2025). Development of Artocarpin-Loaded Chitosan Particles for Controlled Release and Inflammatory Application. Polymers, 18.","DOI":"10.3390\/polym18010008"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/j.ejpb.2007.03.019","article-title":"Targeted transfollicular delivery of artocarpin extract from Artocarpus incisus by means of microparticles","volume":"67","author":"Pitaksuteepong","year":"2007","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_15","first-page":"1","article-title":"Depigmenting action of a nanoemulsion containing heartwood extract of Artocarpus incisus on UVB-induced hyperpigmentation in C57BL\/6 mice","volume":"62","author":"Buranajaree","year":"2011","journal-title":"J. Cosmet. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"725","DOI":"10.3889\/oamjms.2022.8589","article-title":"Wound Healing Activity of Nanoemulgel Containing Artocarpus lakoocha Roxb. Extract on Burns Model in Rat","volume":"10","author":"Tanjung","year":"2022","journal-title":"Open Access Maced. J. Med. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.addr.2009.09.004","article-title":"Biodegradation, Biodistribution and Toxicity of Chitosan","volume":"62","author":"Kean","year":"2010","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Iacob, A.T., Lupascu, F.G., Apotrosoaei, M., Vasincu, I.M., Tauser, R.G., Lupascu, D., Giusca, S.E., Caruntu, I.D., and Profire, L. (2021). Recent Biomedical Approaches for Chitosan-Based Materials as Drug Delivery Nanocarriers. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13040587"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/j.progpolymsci.2011.02.001","article-title":"Chitosan\u2014A Versatile Semi-Synthetic Polymer in Biomedical Applications","volume":"36","author":"Dash","year":"2011","journal-title":"Prog. Polym. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.3390\/md13031133","article-title":"Chitin and Chitosan Preparation from Marine Sources: Structure, Properties and Applications","volume":"13","author":"Younes","year":"2015","journal-title":"Mar. Drugs"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"406","DOI":"10.31083\/ph.2019.8166","article-title":"Oxidative Stress Modulation Induced by Chitosan\u2013Glutathione Nanoparticles in Chondrocytes","volume":"74","author":"Macay","year":"2019","journal-title":"Pharmazie"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Park, S., Kim, B.K., and Park, S.K. (2022). Effects of Fisetin on Oxidative Stress and Age-Related Diseases. Pharmaceuticals, 15.","DOI":"10.3390\/ph15121528"},{"key":"ref_23","first-page":"257","article-title":"Formulation of Chitosan Patch Incorporating Artocarpus altilis Heartwood Extract for Improving Hyperpigmentation","volume":"68","author":"Kwankaew","year":"2017","journal-title":"J. Cosmet. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"125328","DOI":"10.1016\/j.foodchem.2019.125328","article-title":"Enhancing the oral bioavailability of curcumin using solid lipid nanoparticles","volume":"302","author":"Ban","year":"2019","journal-title":"Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Mei, J., Xiao, N., Xi, Y., Chen, X., Zha, X., Cui, L., Yan, F., Xue, R., Wang, Y., and Ma, Y. (2025). Regulation of apoptosis and interaction with cartilage degeneration in osteoarthritis. Front. Cell Dev. Biol., 13.","DOI":"10.3389\/fcell.2025.1571448"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"105642","DOI":"10.1016\/j.jbspin.2023.105642","article-title":"Role of Autophagy in Mitigating Osteoarthritis via Regulation of Chondrocyte Apoptosis","volume":"91","author":"Kong","year":"2024","journal-title":"Jt. Bone Spine"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Liu, L., Luo, P., Yang, M., Wang, J., Hou, W., and Xu, P. (2022). Role of Oxidative Stress in Knee Osteoarthritis Development. Front. Mol. Biosci., 9.","DOI":"10.3389\/fmolb.2022.1001212"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Choi, M.C., Jo, J., Park, J., Kang, H.K., and Park, Y. (2019). NF-\u03baB Signaling Pathways in Osteoarthritic Cartilage Destruction. Cells, 8.","DOI":"10.3390\/cells8070734"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3735","DOI":"10.2147\/DDDT.S314767","article-title":"Bardoxolone-Methyl Prevents Oxidative Stress-Mediated Apoptosis and Extracellular Matrix Degradation In Vitro and Alleviates Osteoarthritis In Vivo","volume":"15","author":"Pang","year":"2021","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_30","first-page":"601","article-title":"Anti-Inflammatory and Analgesic Effects of Artocarpin-Rich Extract and Artocarpin","volume":"6","author":"Bazmi","year":"2022","journal-title":"J. Angiother."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Rawangkan, A., Wongsirisin, P., Pook-In, G., Siriphap, A., Yosboonruang, A., Kiddee, A., Chuerduangphui, J., Reukngam, N., Duangjai, A., and Saokaew, S. (2022). Dinactin: A new antitumor antibiotic with cell cycle progression and cancer stemness inhibiting activities in lung cancer. 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