{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:08:00Z","timestamp":1788948480340,"version":"build-2803163510"},"reference-count":88,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008986","name":"Natural Health Product Research Alliance, University of Guelph","doi-asserted-by":"publisher","award":["053312"],"award-info":[{"award-number":["053312"]}],"id":[{"id":"10.13039\/100008986","id-type":"DOI","asserted-by":"publisher"}]},{"award":["053312"],"award-info":[{"award-number":["053312"]}],"id":[{"id":"https:\/\/ror.org\/01r7awg59","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Camu-camu (Myrciaria dubia) is an Amazonian shrub associated with riverine ecosystems and is recognized for its high vitamin C content and a broad range of nutritional and specialized metabolites. Previous studies have focused on specific metabolites or bioactive metabolites associated with health issues. Therefore, a comprehensive metabolomic survey using multiple analytical methods islacking. To address this gap in the scientific literature, we prepared a focused metabolomic inventory by integrating curated literature records with proton nuclear magnetic resonance (1H NMR) and liquid chromatography\u2013mass spectrometry (LC-MS) observations from berry materials, including pulp, skin\/peel, and seeds. Ourstudy added 107 metabolite annotation records that were not in the literature. The integration of the literature-derived and ourdatasets yielded a combined inventory of 260 metabolites. Seven solvents were compared using 1H NMR spectroscopy, and CH3OH:CD3OD (9:1, v\/v) provided the broadest assigned coverage. LC-MS expanded the detection of lower-abundance and structurally diverse features, particularly phospholipids, quaternary ammonium compounds, organic acids, amino acid derivatives, and related metabolites. Compound-level and class-level assignments were distinguished, and interpretations were limited to the confidence supported by the available analytical evidence. The resulting inventory provides an expanded foundation for camu-camu quality evaluation, solvent selection, clinical trials, and targeted functional food research.<\/jats:p>","DOI":"10.3390\/molecules31183167","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:24:02Z","timestamp":1788945842000},"page":"3167","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Assembling an Expanded Metabolome for Camu-Camu (Myrciaria Dubia): Bioactive and Nutritional Compounds Relevant to Functional Food Research"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5181-005X","authenticated-orcid":false,"given":"Subramanyam","family":"Ragupathy","sequence":"first","affiliation":[{"name":"College of Biological Science, University of Guelph, Guelph, ON N1G 2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9226-447X","authenticated-orcid":false,"given":"Varathan","family":"Vinayagam","sequence":"additional","affiliation":[{"name":"College of Biological Science, University of Guelph, Guelph, ON N1G 2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9427-7013","authenticated-orcid":false,"given":"Velautham","family":"Saravanan","sequence":"additional","affiliation":[{"name":"College of Biological Science, University of Guelph, Guelph, ON N1G 2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7948-2257","authenticated-orcid":false,"given":"Arunachalam","family":"Thirugnanasambandam","sequence":"additional","affiliation":[{"name":"College of Biological Science, University of Guelph, Guelph, ON N1G 2W1, Canada"},{"name":"Biological and Life Sciences, Canadian Light Source, Saskatoon, SK S7N 2V3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8707-7769","authenticated-orcid":false,"given":"Steven G.","family":"Newmaster","sequence":"additional","affiliation":[{"name":"College of Biological Science, University of Guelph, Guelph, ON N1G 2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Tropical American Myrtaceae","volume":"29","author":"McVaugh","year":"1963","journal-title":"Fieldiana Bot."},{"key":"ref_2","first-page":"55","article-title":"Botany of the Guyana Highland. 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