{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:03:50Z","timestamp":1790053430907,"version":"4.0.1"},"reference-count":49,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Slovak Research and Development Agency","award":["APVV-23-0401"],"award-info":[{"award-number":["APVV-23-0401"]}],"id":[{"id":"https:\/\/ror.org\/037nx0e70","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Slovak Research and Development Agency","award":["VEGA 2\/0022\/24"],"award-info":[{"award-number":["VEGA 2\/0022\/24"]}],"id":[{"id":"https:\/\/ror.org\/037nx0e70","id-type":"ROR","asserted-by":"crossref"}]},{"award":["APVV-23-0401"],"award-info":[{"award-number":["APVV-23-0401"]}],"id":[{"id":"https:\/\/ror.org\/037nx0e70","id-type":"ROR","asserted-by":"publisher"}]},{"award":["VEGA 2\/0022\/24"],"award-info":[{"award-number":["VEGA 2\/0022\/24"]}],"id":[{"id":"https:\/\/ror.org\/037nx0e70","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["313011V578"],"award-info":[{"award-number":["313011V578"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"award":["313011V578"],"award-info":[{"award-number":["313011V578"]}],"id":[{"id":"https:\/\/ror.org\/01rkhv978","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Ministry of Education, Research, Development and Youth of the Slovak Republic"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study evaluated Leptospermum scoparium, (manuka) essential oil (LSEO, for its antibacterial activity, cyto\/genotoxicity, antioxidant potential, and its effects on wound closure, reflecting the growing interest in natural compounds with antimicrobial and potential wound-related cellular effects. Analyses were performed on the human keratinocyte cell line HaCaT. The genotoxic effect and the scratch assay of LSEO on human keratinocytes are described for the first time. The results revealed that LSEO did not induce significant DNA damage in vitro after 24 h of treatment. Moreover, treating HaCaT cells with LSEO increased the total antioxidant status level and enhanced wound closure at a concentration of 0.008 \u00b5g\/mL. Additionally, the inhibitory effect of LSEO against several multidrug-resistant bacterial strains, including Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, Enterobacter cloacae, and Staphylococcus aureus, was investigated. LSEO exhibited strong antibacterial activity, with inhibition zones ranging from 25 to 44 mm. The minimal inhibitory concentration and minimal bactericidal concentration of LSEO ranged from 0.10 to 0.5 \u03bcg\/mL and from 0.25 to 0.75 \u03bcg\/mL, respectively. The findings indicate that LSEO exhibits antibacterial activity against the tested multidrug-resistant bacterial isolates and may warrant further investigation as a potential antimicrobial agent.<\/jats:p>","DOI":"10.3390\/molecules31183304","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:12:09Z","timestamp":1789999929000},"page":"3304","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Leptospermum scoparium Essential Oil: Antibacterial Properties, Cyto\/Genotoxic Effects, Antioxidant Activity, and Wound-Healing Potential In Vitro"],"prefix":"10.3390","volume":"31","author":[{"given":"M\u00e1ria","family":"Bu\u010dkov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 21, 84551 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Pu\u0161k\u00e1rov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 21, 84551 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3597-3248","authenticated-orcid":false,"given":"Dominika","family":"Galov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 21, 84551 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6316-1934","authenticated-orcid":false,"given":"Marek","family":"Straka","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Faculty of Medicine, Comenius University and University Hospital Bratislava, 81108 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"Such\u00e1nkov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Faculty of Medicine, Comenius University and University Hospital Bratislava, 81108 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9115-9409","authenticated-orcid":false,"given":"Domenico","family":"Pangallo","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 21, 84551 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2758-2325","authenticated-orcid":false,"given":"Monika","family":"\u0160ramkov\u00e1","sequence":"additional","affiliation":[{"name":"Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6412-7962","authenticated-orcid":false,"given":"Katar\u00edna","family":"Kozics","sequence":"additional","affiliation":[{"name":"Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.ijbiomac.2018.10.115","article-title":"Wound Dressing Based on Electrospun PVA\/Chitosan\/Starch Nanofibrous Mats: Fabrication, Antibacterial and Cytocompatibility Evaluation and in Vitro Healing Assay","volume":"122","author":"Adeli","year":"2019","journal-title":"Int. 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