{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T04:57:58Z","timestamp":1790657878816,"version":"4.1.0"},"reference-count":38,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>An acidic heteropolysaccharide, named LCP-90-3, with a relative molecular weight of 14.02 kDa, was isolated and purified from the traditional heat-clearing Chinese medicine, Lysimachia christinae Hance. The monosaccharide residue analysis indicated that LCP-90-3 was composed of glucuronic acid, mannose, and arabinose in a molar ratio of 79.2: 3.6: 17.2. Linkage analysis further indicated that LCP-90-3 was mainly composed of 1,4-GlcpA, 1,4,6-GlcpA, 1,2-Araf, T-Manp, 1,3-Araf, and 1,4-Manp residues. Furthermore, LCP-90-3 demonstrated potent anti-inflammatory activity by significantly inhibiting the secretion of nitric oxide (NO), tumor necrosis factor-\u03b1 (TNF-\u03b1), and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-treated ANA-1 cells. Interestingly, oral administration of LCP-90-3 at doses of 20, 40, and 80 mg\/kg effectively mitigated LPS-induced acute liver injury in vivo by suppressing the overexpression of inflammatory mediators (NO, TNF-\u03b1, and IL-6), aminotransferases (ALT and AST) in serum, and markers of antioxidant capacity (MDA, CAT, and SOD) in the liver. Overall, these findings highlight LCP-90-3 as a novel leading polysaccharide for new drug candidate development against liver injury through modulation of inflammatory responses and oxidative stress.<\/jats:p>","DOI":"10.3390\/molecules31193432","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T11:35:59Z","timestamp":1790595359000},"page":"3432","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preliminary Structural Characterization of an Anti-Inflammatory Polysaccharide from Lysimachia christinae and Its Preventive Effect on Lipopolysaccharide-Induced Acute Liver Injury in Mice"],"prefix":"10.3390","volume":"31","author":[{"given":"Yuanyuan","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4879-7690","authenticated-orcid":false,"given":"Zhou","family":"Hong","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Ningxia Vocational and Technical University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Xiwei","family":"Jia","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Ningxia Vocational and Technical University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Meimei","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Hao","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Liming","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Lishuang","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang 524023, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/s41392-024-02072-z","article-title":"Liver diseases: Epidemiology, causes, trends and predictions","volume":"5","author":"Gan","year":"2025","journal-title":"Signal. 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