{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:11:36Z","timestamp":1792789896785,"version":"4.5.0"},"reference-count":32,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Alchemist Project of the Korea Evaluation Institute of Industrial Technology","award":["KEIT 20018560"],"award-info":[{"award-number":["KEIT 20018560"]}],"id":[{"id":"https:\/\/ror.org\/03z9cwa38","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Alchemist Project of the Korea Evaluation Institute of Industrial Technology","award":["NTIS 1415184668"],"award-info":[{"award-number":["NTIS 1415184668"]}],"id":[{"id":"https:\/\/ror.org\/03z9cwa38","id-type":"ROR","asserted-by":"crossref"}]},{"award":["KEIT 20018560"],"award-info":[{"award-number":["KEIT 20018560"]}],"id":[{"id":"https:\/\/ror.org\/03z9cwa38","id-type":"ROR","asserted-by":"publisher"}]},{"award":["NTIS 1415184668"],"award-info":[{"award-number":["NTIS 1415184668"]}],"id":[{"id":"https:\/\/ror.org\/03z9cwa38","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Ministry of Trade, Industry & Energy","id":[{"id":"https:\/\/ror.org\/008nkqk13","id-type":"ROR","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/008nkqk13","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Plant-derived extracellular vesicle-like nanoparticles (EVNPs) have emerged as bioactive nanomaterials with potential applications in skin biology; however, their isolation from complex plant matrices and their integrated biological effects remain insufficiently characterized. In this study, hemp seed-derived vesicle-like nanoparticles (hs- extracellular VLNPs) were isolated using a multi-step workflow combining pre-clarification, sequential filtration, electrokinetic capture, and tangential flow filtration. The isolated hs-VLNPs exhibited membrane-enclosed vesicle-like morphology, a nanoscale size distribution predominantly within 100\u2013200 nm, and a negative surface charge. hs-VLNPs maintained cellular viability within the concentration range used for most functional assays and exhibited multiple biological activities associated with skin cellular homeostasis. In human dermal fibroblasts, hs-VLNPs increased procollagen type I production and attenuated UVB-induced matrix metalloproteinase-1 (MMP-1) expression following UVB exposure. In HaCaT keratinocytes, hs-VLNPs enhanced scratch closure under non-cytotoxic treatment conditions. Inflammatory mediator responses were cell- and mediator-dependent, with differential effects observed in RAW264.7 macrophages and human dermal fibroblasts rather than uniform suppression of inflammatory mediators. In addition, hs-VLNPs reduced \u03b1-melanocyte-stimulating hormone (\u03b1-MSH)-induced melanin production while exhibiting little direct inhibition of tyrosinase activity, suggesting that the anti-melanogenic effect may involve cellular regulatory mechanisms rather than direct enzymatic inhibition. Collectively, these findings demonstrate that electrokinetic capture combined with membrane-based filtration enables the enrichment of biologically active vesicle-like nanoparticles from hemp seeds. Rather than exerting a single biological effect, hs-VLNPs modulated multiple interconnected processes involving extracellular matrix homeostasis, cellular migration, immune responses, and melanogenesis, providing a foundation for further investigation of hemp seed-derived EVNPs as plant-derived nanomaterials for skin-related applications.<\/jats:p>","DOI":"10.3390\/molecules31193455","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T11:35:59Z","timestamp":1790595359000},"page":"3455","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hemp Seed-Derived Vesicle-like Nanoparticles Modulate Dermal Collagen Homeostasis and Attenuate UVB-Induced Photoaging-Related Responses"],"prefix":"10.3390","volume":"31","author":[{"given":"Hannah S.","family":"Park","sequence":"first","affiliation":[{"name":"Engineering Research Center for Biofluid Biopsy, Seoul 02841, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2611-5610","authenticated-orcid":false,"given":"Sehyun","family":"Shin","sequence":"additional","affiliation":[{"name":"Engineering Research Center for Biofluid Biopsy, Seoul 02841, Republic of Korea"},{"name":"School of Mechanical Engineering, Korea University, Seoul 02841, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/jidsymp.2009.8","article-title":"Matrix-degrading metalloproteinases in photoaging","volume":"14","author":"Quan","year":"2009","journal-title":"J. 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