{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:02:35Z","timestamp":1790672555231,"version":"4.1.0"},"reference-count":100,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Qatar Research and Development Innovation Council","award":["ARG01-0531-230424"],"award-info":[{"award-number":["ARG01-0531-230424"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Lung and ovarian cancers account for substantial cancer morbidity and mortality worldwide. Plant-derived natural products represent promising alternatives in cancer therapy because of their structural diversity and ability to target multiple signaling pathways. Prosopis species are ethnomedicinal plants traditionally known for their antimicrobial, anti-inflammatory, and antioxidant activities; however, their anticancer potential remains poorly explored. This study evaluates the anticancer properties of extracts of Prosopis cineraria, Prosopis farcta, and Prosopis juliflora, the Prosopis species growing in Qatar, against human lung (A549) and ovarian (SK-OV-3) cancer cells. Gas chromatography\u2013mass spectrometry (GC\u2013MS) was used to characterize their phytochemical composition. The cytotoxicity and anti-migratory activity of the three extracts were evaluated. Western blot analysis was performed to investigate the molecular effects of the extracts on proliferation- and apoptosis-related proteins. GC\u2013MS profiling identified diverse low-molecular-weight bioactive compounds across the extracts, including several compounds detected only in P. juliflora, which exhibited the most potent cytotoxicity toward A549 and SK-OV-3 cells. All three extracts inhibited cancer cell migration, with P. juliflora showing the strongest effect. Furthermore, P. juliflora reduced the expression of proliferation and survival markers and increased the expression of pro-apoptotic proteins. These findings identify P. juliflora as the most active of the three tested Prosopis extracts and support further investigation of its in vitro anticancer potential.<\/jats:p>","DOI":"10.3390\/molecules31193464","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:12:42Z","timestamp":1790669562000},"page":"3464","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Profiles and Anticancer Activity of the Three Prosopis Species Growing in Qatar Against Lung and Ovarian Cancer Cells"],"prefix":"10.3390","volume":"31","author":[{"given":"Salma M. S.","family":"Ahmad","sequence":"first","affiliation":[{"name":"Biomedical Research Center (BRC), QU Health, Qatar University, Doha P.O. Box 2713, Qatar"},{"name":"Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zainab","family":"Farooq","sequence":"additional","affiliation":[{"name":"Biomedical Research Center (BRC), QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4753-7492","authenticated-orcid":false,"given":"Maysa Z.","family":"Masalmeh","sequence":"additional","affiliation":[{"name":"Biomedical Research Center (BRC), QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Iman","family":"Saleh","sequence":"additional","affiliation":[{"name":"Biological Science Program, Department of Biological and Environmental Sciences, College of Art and Science, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Fatiha","family":"Seglab","sequence":"additional","affiliation":[{"name":"Biomedical Research Center (BRC), QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Layla","family":"Al-Mansoori","sequence":"additional","affiliation":[{"name":"Biomedical Research Center (BRC), QU Health, Qatar University, Doha P.O. Box 2713, Qatar"},{"name":"Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0417-3414","authenticated-orcid":false,"given":"Mohammed H.","family":"Abu-Dieyeh","sequence":"additional","affiliation":[{"name":"Biological Science Program, Department of Biological and Environmental Sciences, College of Art and Science, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3524-7962","authenticated-orcid":false,"given":"Abdullah A.","family":"Shaito","sequence":"additional","affiliation":[{"name":"Biomedical Research Center (BRC), QU Health, Qatar University, Doha P.O. Box 2713, Qatar"},{"name":"Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"},{"name":"College of Medicine, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"ref_1","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Shaito, A.A., Omairi, I., Al-Thani, N., Seglab, F., Ad-Darwish, E., Kobeissy, F., and Nasreddine, S. (2024). 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