{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:56:02Z","timestamp":1790942162045,"version":"4.1.0"},"reference-count":47,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["24\u201345-00071"],"award-info":[{"award-number":["24\u201345-00071"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"award":["24\u201345-00071"],"award-info":[{"award-number":["24\u201345-00071"]}],"id":[{"id":"https:\/\/ror.org\/03y2gwe85","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82361138563"],"award-info":[{"award-number":["82361138563"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["82361138563"],"award-info":[{"award-number":["82361138563"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>1-Isobutylpurine-2,6-diones possess a broad spectrum of pharmacological activities, highlighting their relevance in the search for new biologically active agents. In this study, we report the synthesis of a series of novel thietane-containing 1-isobutylpurine-2,6-dione derivatives. A crucial aspect of this work is the detailed stereochemical and conformational analysis of the thietanyl substituent. For the first time, the unambiguous assignment of axial and equatorial protons within the thietane ring was achieved using 2D NMR spectroscopy, exemplified by a comprehensive study of the starting substance. Furthermore, the newly synthesized compounds were evaluated for their antiplatelet activity, revealing inhibition of platelet aggregation exceeding that of acetylsalicylic acid. Based on structural analogy to an established antiplatelet agent, we hypothesize that the observed biological effect is mediated via the inhibition of the glycoprotein IIb\/IIIa (GPIIb\/IIIa) receptor complex. These findings demonstrate that the incorporation of a thietane fragment into the 1-isobutylxanthine scaffold yields promising candidates for the development of novel GPIIb\/IIIa-targeted antiplatelet therapeutics.<\/jats:p>","DOI":"10.3390\/molecules31193521","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:21:49Z","timestamp":1790940109000},"page":"3521","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stereochemical 2D NMR Assignment of Thietane, Synthesis and Antiplatelet Activity of Novel Thietane-Containing 1-Isobutylpurine-2,6-dione Derivatives as Potential GPIIb\/IIIa Inhibitors"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0564-4246","authenticated-orcid":false,"given":"Ferkat A.","family":"Khaliullin","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Bashkir State Medical University, Lenina Str., 3, Ufa 450008, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Zhekshen K.","family":"Mamatov","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Business Organization and Pharmacognosy, Osh State University, Alymbek Datka Str., 331, Osh 723500, Kyrgyzstan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5432-1975","authenticated-orcid":false,"given":"Iuliia V.","family":"Shabalina","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Bashkir State Medical University, Lenina Str., 3, Ufa 450008, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hangzhou Normal University, Yuhangtan, 2318, Hangzhou 310030, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9223-508X","authenticated-orcid":false,"given":"Aleksandr N.","family":"Lobov","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Bashkir State Medical University, Lenina Str., 3, Ufa 450008, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Elena E.","family":"Klen","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Bashkir State Medical University, Lenina Str., 3, Ufa 450008, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Kudaiberdi G.","family":"Kozhobekov","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Business Organization and Pharmacognosy, Osh State University, Alymbek Datka Str., 331, Osh 723500, Kyrgyzstan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Dmitry A.","family":"Kudlay","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119435, Russia"},{"name":"Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Leninskie Gory 1, Building 73, Moscow 119234, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"project-administration","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9302-499X","authenticated-orcid":false,"given":"Aleksandr V.","family":"Samorodov","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Bashkir State Medical University, Lenina Str., 3, Ufa 450008, Russia"},{"name":"Department of Pharmacology, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119435, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1016\/S0022-3565(25)24562-1","article-title":"Differential 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