{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:50:04Z","timestamp":1791435004455,"version":"4.3.3"},"reference-count":35,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University of Bia\u0142ystok","award":["B.SUB.25.335"],"award-info":[{"award-number":["B.SUB.25.335"]}],"id":[{"id":"https:\/\/ror.org\/00y4ya841","id-type":"ROR","asserted-by":"crossref"}]},{"award":["B.SUB.25.335"],"award-info":[{"award-number":["B.SUB.25.335"]}],"id":[{"id":"https:\/\/ror.org\/00y4ya841","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001870","name":"Foundation for Polish Science","doi-asserted-by":"publisher","award":["START 37.2026"],"award-info":[{"award-number":["START 37.2026"]}],"id":[{"id":"10.13039\/501100001870","id-type":"DOI","asserted-by":"publisher"}]},{"award":["START 37.2026"],"award-info":[{"award-number":["START 37.2026"]}],"id":[{"id":"https:\/\/ror.org\/048zd9m77","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Cannabigerol (CBG) exerts vasorelaxant effects in human pulmonary arteries and lowers blood pressure (BP) in normotensive mice. However, data on the cardiovascular effects of CBG in hypertension and platelet function remain limited. Consequently, the aims of our study were as follows: to assess the effect of CBG on BP and heart rate (HR) in spontaneously hypertensive rats (SHR) and their normotensive controls (Wistar Kyoto rats; WKY); to investigate the vasorelaxant potential of CBG in small mesenteric arteries (sMAs) and the aorta; and to investigate the effect of CBG on platelet function in human blood. Acute CBG administration (1, 3, and 10 mg\/kg) dose-dependently lowered BP and HR in SHR and WKY rats, with comparable effects between strains (except for a greater reduction in systolic BP in SHR). Furthermore, experiments on pithed rats support the possibility of both central and peripheral contributions to this hypotensive effect. The peripheral component is supported by CBG-induced vasorelaxation in sMAs and aortas, which was more pronounced in sMAs and in WKY rats. Additionally, we demonstrated that CBG inhibits platelet aggregation (collagen-induced) and secretion and reduces thrombus formation and platelet procoagulant response under arterial flow conditions. Taken together, these data indicate that CBG exerts multifaceted cardiovascular effects.<\/jats:p>","DOI":"10.3390\/molecules31193555","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:34:18Z","timestamp":1791279258000},"page":"3555","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cannabigerol Modulates Blood Pressure and Vascular Reactivity in a Rat Model of Hypertension and Platelet Responses in Human Blood In Vitro"],"prefix":"10.3390","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5986-3757","authenticated-orcid":false,"given":"Anna","family":"Krzy\u017cewska","sequence":"first","affiliation":[{"name":"Department of Experimental Physiology and Pathophysiology, Medical University of Bia\u0142ystok, Mickiewicz Str. 2A, 15-222 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6894-0953","authenticated-orcid":false,"given":"Krzysztof","family":"Mi\u0144czuk","sequence":"additional","affiliation":[{"name":"Department of Experimental Physiology and Pathophysiology, Medical University of Bia\u0142ystok, Mickiewicz Str. 2A, 15-222 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5403-9467","authenticated-orcid":false,"given":"Tomasz","family":"Misztal","sequence":"additional","affiliation":[{"name":"Department of Physical Chemistry, Medical University of Bia\u0142ystok, Mickiewicz Str. 2A, 15-222 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7374-0999","authenticated-orcid":false,"given":"Monika","family":"Kloza","sequence":"additional","affiliation":[{"name":"Department of Experimental Physiology and Pathophysiology, Medical University of Bia\u0142ystok, Mickiewicz Str. 2A, 15-222 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Koz\u0142owska","sequence":"additional","affiliation":[{"name":"Department of Experimental Physiology and Pathophysiology, Medical University of Bia\u0142ystok, Mickiewicz Str. 2A, 15-222 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Remiszewski, P., and Malinowska, B. (2022). Why Multitarget Vasodilatory (Endo)cannabinoids Are Not Effective as Antihypertensive Compounds after Chronic Administration: Comparison of Their Effects on Systemic and Pulmonary Hypertension. Pharmaceuticals, 15.","DOI":"10.3390\/ph15091119"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Jastrz\u0105b, A., Jarocka-Karpowicz, I., and Skrzydlewska, E. (2022). The Origin and Biomedical Relevance of Cannabigerol. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23147929"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/j.1476-5381.2009.00515.x","article-title":"Evidence that the plant cannabinoid cannabigerol is a highly potent alpha2-adrenoceptor agonist and moderately potent 5HT1A receptor antagonist","volume":"159","author":"Cascio","year":"2010","journal-title":"Br. J. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Vernail, V.L., Bingaman, S.S., Silberman, Y., Raup-Konsavage, W.M., Vrana, K.E., and Arnold, A.C. (2022). Acute Cannabigerol Administration Lowers Blood Pressure in Mice. Front. Physiol., 13.","DOI":"10.3389\/fphys.2022.871962"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Krzy\u017cewska, A., Kloza, M., and Koz\u0142owska, H. (2025). Comprehensive mini-review: Therapeutic potential of cannabigerol\u2014Focus on the cardiovascular system. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1561385"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"117383","DOI":"10.1016\/j.bcp.2025.117383","article-title":"Ex vivo study of the vasorelaxant activity induced by cannabigerol in human pulmonary artery- the role of endothelium, sex and selected clinical factors","volume":"242","author":"Kloza","year":"2025","journal-title":"Biochem. Pharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1111\/j.2042-7158.1989.tb06345.x","article-title":"The inhibitory effects of cannabinoids, the active constituents of Cannabis sativa L. on human and rabbit platelet aggregation","volume":"41","author":"Formukong","year":"1989","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"153460","DOI":"10.1016\/j.tox.2023.153460","article-title":"Safety assessment and redox status in rats after chronic exposure to cannabidiol and cannabigerol","volume":"488","author":"Polanska","year":"2023","journal-title":"Toxicology"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Kicman, A., and Toczek, M. (2020). The Effects of Cannabidiol, a Non-Intoxicating Compound of Cannabis, on the Cardiovascular System in Health and Disease. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21186740"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Weresa, J., P\u0119dzi\u0144ska-Betiuk, A., Mi\u0144czuk, K., Malinowska, B., and Schlicker, E. (2022). Why Do Marijuana and Synthetic Cannabimimetics Induce Acute Myocardial Infarction in Healthy Young People?. Cells, 11.","DOI":"10.3390\/cells11071142"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1097\/CRD.0000000000000566","article-title":"Cardiovascular Effects of Cannabinoids","volume":"33","author":"Wang","year":"2025","journal-title":"Cardiol. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1161\/01.HYP.0000193538.20705.23","article-title":"The prothrombotic paradox of hypertension: Role of the renin-angiotensin and kallikrein-kinin systems","volume":"46","author":"Dielis","year":"2005","journal-title":"Hypertension"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1586\/14779072.5.3.441","article-title":"Hypertension: Endothelial dysfunction, the prothrombotic state and antithrombotic therapy","volume":"5","author":"Kakar","year":"2007","journal-title":"Expert Rev. Cardiovasc. Ther."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Toczek, M., Schlicker, E., Remiszewski, P., and Malinowska, B. (2024). Function of Presynaptic Inhibitory Cannabinoid CB1 Receptors in Spontaneously Hypertensive Rats and Its Modification by Enhanced Endocannabinoid Tone. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25020858"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e87","DOI":"10.1161\/HYP.0000000000000090","article-title":"Animal Models of Hypertension: A Scientific Statement From the American Heart Association","volume":"73","author":"Lerman","year":"2019","journal-title":"Hypertension"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1111\/bph.15650","article-title":"Rodent models of hypertension","volume":"179","author":"Jama","year":"2022","journal-title":"Br. J. Pharmacol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1111\/j.1476-5381.2009.00315.x","article-title":"Urethane, but not pentobarbitone, attenuates presynaptic receptor function in rats: A contribution to the choice of anaesthetic","volume":"157","author":"Kurz","year":"2009","journal-title":"Br. J. Pharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e01003","DOI":"10.1002\/prp2.1003","article-title":"The signaling and selectivity of \u03b1-adrenoceptor agonists for the human \u03b12A, \u03b12B and \u03b12C-adrenoceptors and comparison with human \u03b11 and \u03b2-adrenoceptors","volume":"10","author":"Proudman","year":"2022","journal-title":"Pharmacol. Res. Perspect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2377","DOI":"10.1038\/s41440-024-01765-w","article-title":"More efficient adaptation of cardiovascular response to repeated restraint in spontaneously hypertensive rats: The role of autonomic nervous system","volume":"47","author":"Behuliak","year":"2024","journal-title":"Hypertens. Res."},{"key":"ref_20","first-page":"3119","article-title":"Mesenteric artery contraction and relaxation studies using automated wire myography","volume":"55","author":"Bridges","year":"2011","journal-title":"J. Vis. Exp."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"598271","DOI":"10.1155\/2014\/598271","article-title":"Endothelial dysfunction in experimental models of arterial hypertension: Cause or consequence?","volume":"2014","author":"Bernatova","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1093\/cvr\/cvv179","article-title":"Cannabidiol causes endothelium-dependent vasorelaxation of human mesenteric arteries via CB1 activation","volume":"107","author":"Stanley","year":"2015","journal-title":"Cardiovasc. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1097\/HJH.0000000000002333","article-title":"Vasodilatory effects of cannabidiol in human pulmonary and rat small mesenteric arteries: Modification by hypertension and the potential pharmacological opportunities","volume":"38","author":"Kloza","year":"2020","journal-title":"J. Hypertens."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Morales, P., Reggio, P.H., and Jagerovic, N. (2017). An Overview on Medicinal Chemistry of Synthetic and Natural Derivatives of Cannabidiol. Front. Pharmacol., 8.","DOI":"10.3389\/fphar.2017.00422"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"De Angelis, V., Koekman, A.C., Weeterings, C., Roest, M., de Groot, P.G., Herczenik, E., and Maas, C. (2014). Endocannabinoids control platelet activation and limit aggregate formation under flow. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0108282"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Kossakowski, R., Schlicker, E., Toczek, M., Weresa, J., and Malinowska, B. (2019). Cannabidiol Affects the Bezold-Jarisch Reflex via TRPV1 and 5-HT3 Receptors and Has Peripheral Sympathomimetic Effects in Spontaneously Hypertensive and Normotensive Rats. Front. Pharmacol., 10.","DOI":"10.3389\/fphar.2019.00500"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"110279","DOI":"10.1016\/j.neuropharm.2024.110279","article-title":"Angiotensin 1-7 injected into the rat paraventricular nucleus of hypothalamus increases blood pressure and heart rate via various receptors","volume":"266","author":"Schlicker","year":"2025","journal-title":"Neuropharmacology"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Baranowska-Kuczko, M., Koz\u0142owska, H., Kloza, M., Kusaczuk, M., Harasim-Symbor, E., Biernacki, M., Kasacka, I., and Malinowska, B. (2021). Vasoprotective Endothelial Effects of Chronic Cannabidiol Treatment and Its Influence on the Endocannabinoid System in Rats with Primary and Secondary Hypertension. Pharmaceuticals, 14.","DOI":"10.3390\/ph14111120"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4257","DOI":"10.1038\/ncomms5257","article-title":"Identification of platelet function defects by multi-parameter assessment of thrombus formation","volume":"5","author":"Swieringa","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_30","unstructured":"Rasband, W.S. (ImageJ, 2016). ImageJ, version 1.54."},{"key":"ref_31","unstructured":"Kenakin, T.P. (2019). A Pharmacology Primer: Techniques for More Effective and Strategic Drug Discovery, Academic Press. [5th ed.]."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Zagzoog, A., Halter, K., Ha, N., Jones, A.M., Andres, R., Kim, A., Michel, D., Alcorn, J., and Laprairie, R.B. (2025). Pharmacokinetics and pharmacodynamics of cannabigerol (CBG) in the C57BL\/6Crl mouse. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1672098"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4544","DOI":"10.1021\/acs.molpharmaceut.4c01357","article-title":"Effect of Quantitative Structural Properties and Drug Formulation in Four Cannabinoids (Cannabidiol, Cannabigerol, Cannabichromene, and Cannabinol) on Their Lymphatic Transport after Enteral Administration in Rats","volume":"22","author":"Housar","year":"2025","journal-title":"Mol. Pharm."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1089\/can.2023.0174","article-title":"Impact of Dietary Fat and Oral Delivery System on Cannabigerol Pharmacokinetics in Adults","volume":"9","author":"Story","year":"2024","journal-title":"Cannabis Cannabinoid Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1007\/978-3-319-77932-4_27","article-title":"Sex-Specific Physiology and Cardiovascular Disease","volume":"1065","author":"Shufelt","year":"2018","journal-title":"Adv. Exp. Med. Biol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/19\/3555\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:12:49Z","timestamp":1791432769000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/31\/19\/3555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,6]]},"references-count":35,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["molecules31193555"],"URL":"https:\/\/doi.org\/10.3390\/molecules31193555","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,6]]}}}