{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T06:36:03Z","timestamp":1775802963936,"version":"3.50.1"},"reference-count":15,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,5,15]],"date-time":"2019-05-15T00:00:00Z","timestamp":1557878400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MPs"],"abstract":"<jats:p>Tetrahymena pyriformis (protozoa) is intensely investigated as a model organism, offering numerous advantages in comprehensive and multidisciplinary studies using morphologic or molecular methods. Since DNA extraction is a vital step of any molecular experiment, here a new mixed surfactant (Sodium dodecyl sulfate (SDS) 20%\/Triton X-100) was adopted for effective DNA extraction from Tetrahymena pyriformis under an easy, fast protocol. The efficiency of this technique was then compared with three widely-used alternative techniques, namely the Chelex 100 matrix, Ammonium pyrrolidine dithiocarbamate (APD) complex and SDS\u2013chloroform methods. DNA extraction was analyzed by pulsed-field gel electrophoresis, spectral measurement, fluorometry (Qubit), restriction enzyme digestion, and polymerase chain reaction. Data analysis revealed that the quantity and quality of the recovered DNA varied depending on the applied DNA extraction method. The new method (SDS 20%\/Triton X-100) was the most efficient for extracting DNA from Tetrahymena pyriformis with high integrity and purity, affordable cost, less time, and suitability for molecular applications.<\/jats:p>","DOI":"10.3390\/mps2020040","type":"journal-article","created":{"date-parts":[[2019,5,15]],"date-time":"2019-05-15T11:37:40Z","timestamp":1557920260000},"page":"40","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis"],"prefix":"10.3390","volume":"2","author":[{"given":"Ezzouhra","family":"El Maaiden","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry and Neurosciences, Department of Biology, University Hassan I, BP 577, 26000 Settat, Morocco"},{"name":"Laboratory of Applied Chemistry and Environment, Department of Chemistry, University Hassan I, BP 577, 26000 Settat, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youssef","family":"El Kharrassi","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Neurosciences, Department of Biology, University Hassan I, BP 577, 26000 Settat, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdel Khalid","family":"Essamadi","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Neurosciences, Department of Biology, University Hassan I, BP 577, 26000 Settat, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khadija","family":"Moustaid","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Chemistry and Environment, Department of Chemistry, University Hassan I, BP 577, 26000 Settat, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boubker","family":"Nasser","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Neurosciences, Department of Biology, University Hassan I, BP 577, 26000 Settat, Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0378-4274(01)00542-2","article-title":"The effect of lofepramine and other related agents on the motility of Tetrahymena pyriformis","volume":"128","author":"Darcy","year":"2002","journal-title":"Toxicol. Lett."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00898-996-0005-0","article-title":"Concanavalin A Receptors and the Chemosensory Behaviour of Tetrahymena thermophila","volume":"1","author":"Leick","year":"1996","journal-title":"Exp. Biol. Online"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.chroma.2007.11.120","article-title":"New hydroxyapatite monolithic column for DNA extraction and its application in the purification of Bacillus subtilis crude lysate","volume":"1183","author":"Yu","year":"2008","journal-title":"J. Chromatogr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3564","DOI":"10.1002\/elps.200406161","article-title":"DNA sequencing and genotyping in miniaturized electrophoresis systems","volume":"25","author":"Kan","year":"2004","journal-title":"Electrophoresis"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Barbosa, C., Nogueira, S., Gadanho, M., and Chaves, S. (2016). DNA extraction: Finding the most suitable method. Molecular Microbial Diagnostic Methods, Academic Press.","DOI":"10.1016\/B978-0-12-416999-9.00007-1"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2891","DOI":"10.1128\/AEM.02234-06","article-title":"SYBR Green real-time PCR method to detect Clostridium botulinum type A","volume":"73","author":"Fenicia","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1111\/j.1432-1033.1979.tb04254.x","article-title":"Characterization of Preribosomal Ribonucleoprotein Particles from Tetrahymena pyriformis","volume":"102","author":"Pousada","year":"1979","journal-title":"Eur. J. Biochem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"506","DOI":"10.2144\/000114018","article-title":"Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material","volume":"54","author":"Walsh","year":"2013","journal-title":"BioTechniques"},{"key":"ref_9","first-page":"123","article-title":"A New Method for Rapid DNA Extraction from Artemia (Branchiopoda, Crustacea)","volume":"6","author":"Manaffar","year":"2010","journal-title":"Int. J. Bioeng. Life Sci."},{"key":"ref_10","unstructured":"Green, M.R., and Sambrook, J. (2012). Molecular cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press. [4th ed.]."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1126\/science.3952500","article-title":"Electrophoretic separations of large DNA molecule by periodic inversion of electric field","volume":"232","author":"Carle","year":"1986","journal-title":"Science"},{"key":"ref_12","first-page":"654","article-title":"Tilapia metallothionein genes: PCR-cloning and gene expression studies","volume":"1731","author":"Cheung","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_13","first-page":"900","article-title":"Macronuclear DNA Molecules of Tetrahymena thermophila","volume":"6","author":"Conover","year":"1986","journal-title":"Mol. Cell. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BF00284863","article-title":"Comparison of the DNA sequences of Tetrahymena macro- and micronuclei","volume":"48","author":"Yao","year":"1974","journal-title":"Chromosoma"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7436","DOI":"10.1073\/pnas.78.12.7436","article-title":"Repeated hexanucleotide C-C-C-C-A-A is present near free ends of macronuclear DNA of Tetrahymena","volume":"78","author":"Yao","year":"1981","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Methods and Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2409-9279\/2\/2\/40\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:52:12Z","timestamp":1760187132000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2409-9279\/2\/2\/40"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,15]]},"references-count":15,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,6]]}},"alternative-id":["mps2020040"],"URL":"https:\/\/doi.org\/10.3390\/mps2020040","relation":{},"ISSN":["2409-9279"],"issn-type":[{"value":"2409-9279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,15]]}}}