{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T18:56:02Z","timestamp":1781895362120,"version":"3.54.5"},"reference-count":14,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,2,6]],"date-time":"2022-02-06T00:00:00Z","timestamp":1644105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["21-15-00111"],"award-info":[{"award-number":["21-15-00111"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MPs"],"abstract":"<jats:p>Cellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in vitro. On the other hand, organotypic culture can be used as a powerful model system and can complement cell culture and in vivo studies in different biomedical applications. Uterine leiomyoma (UL) is a very common benign tumor and often leads to many reproductive complications. Herein we describe a fast and reliable method of isolation and UL primary cells culturing along with the development of a UL organotypic model. We propose the usage of UL primary cells in experimental work at a first passage to prevent loss of driver mutations in MED12 and HMGA2 genes. New optimized conditions for the growth and maintenance of 2D and 3D models of uterine leiomyoma in vitro are suggested.<\/jats:p>","DOI":"10.3390\/mps5010016","type":"journal-article","created":{"date-parts":[[2022,2,6]],"date-time":"2022-02-06T20:38:40Z","timestamp":1644179920000},"page":"16","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma"],"prefix":"10.3390","volume":"5","author":[{"given":"Natalia","family":"Shved","sequence":"first","affiliation":[{"name":"Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, MendeleevskayaLine 3, 199034 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Egorova","sequence":"additional","affiliation":[{"name":"Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, MendeleevskayaLine 3, 199034 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Osinovskaya","sequence":"additional","affiliation":[{"name":"Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, MendeleevskayaLine 3, 199034 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2487-2423","authenticated-orcid":false,"given":"Anton","family":"Kiselev","sequence":"additional","affiliation":[{"name":"Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, MendeleevskayaLine 3, 199034 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,6]]},"reference":[{"key":"ref_1","first-page":"165","article-title":"Action of progesterone receptor modulators on uterine leiomyomas","volume":"35","author":"Ohara","year":"2008","journal-title":"Clin. Exp. Obstet. Gynecol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.biopha.2012.02.003","article-title":"Gestrinone inhibits growth of human uterine leiomyoma may relate to activity regulation of ER\u03b1, Src and P38 MAPK","volume":"66","author":"Zhu","year":"2012","journal-title":"Biomed. Pharmacother."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1093\/humupd\/dmt058","article-title":"Proceedings from the third national institutes of health international congress on advances in uterine leiomyoma research: Comprehensive review, conference summary and future recommendations","volume":"20","author":"Segars","year":"2014","journal-title":"Hum. Reprod. Update"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1134\/S1022795421090118","article-title":"Molecular Genetic Basis and Prospects of Gene Therapy of Uterine Leiomyoma","volume":"57","author":"Shtykalova","year":"2021","journal-title":"Russ. J. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1002\/gcc.22142","article-title":"Cell cultures in uterine leiomyomas: Rapid disappearance of cells carrying MED12 mutations","volume":"53","author":"Tadayyon","year":"2014","journal-title":"Genes Chromosomes Cancer"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s13039-015-0177-9","article-title":"A rare coincidence of different types of driver mutations among uterine leiomyomas (UL)","volume":"8","author":"Holzmann","year":"2015","journal-title":"Mol. 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Reprod."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5212","DOI":"10.1038\/s41598-020-62158-w","article-title":"Organotypic culture as a research and preclinical model to study uterine leiomyomas","volume":"10","author":"Salas","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1097\/PGP.0000000000000255","article-title":"Frequency and Spectrum of MED12 Exon 2 Mutations in Multiple Versus Solitary Uterine Leiomyomas From Russian Patients","volume":"35","author":"Osinovskaya","year":"2016","journal-title":"Int. J. Gynecol. Pathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1136\/jclinpath-2016-203976","article-title":"Catechol-O-methyltransferase Val158Met polymorphism is associated with increased risk of multiple uterine leiomyomas either positive or negative for MED12 exon 2 mutations","volume":"70","author":"Dzhemlikhanova","year":"2017","journal-title":"J. Clin. Pathol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/j.fertnstert.2014.05.013","article-title":"Tranilast, an orally active antiallergic compound, inhibits extracellular matrix production in human uterine leiomyoma and myometrial cells","volume":"102","author":"Islam","year":"2014","journal-title":"Fertil. Steril."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1478-811X-8-10","article-title":"Human uterine leiomyoma-derived fibroblasts stimulate uterine leiomyoma cell proliferation and collagen type I production, and activate RTKs and TGF beta receptor signaling in coculture","volume":"8","author":"Moore","year":"2010","journal-title":"Cell Commun. Signal."}],"container-title":["Methods and Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2409-9279\/5\/1\/16\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:15:08Z","timestamp":1760134508000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2409-9279\/5\/1\/16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,6]]},"references-count":14,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["mps5010016"],"URL":"https:\/\/doi.org\/10.3390\/mps5010016","relation":{},"ISSN":["2409-9279"],"issn-type":[{"value":"2409-9279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,6]]}}}