{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:37:57Z","timestamp":1769506677532,"version":"3.49.0"},"reference-count":39,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,7,10]],"date-time":"2022-07-10T00:00:00Z","timestamp":1657411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Collaborative Research Infrastructure Strategy (NCRIS)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MPs"],"abstract":"<jats:p>The molecular analysis of small or rare patient tissue samples is challenging and often limited by available technologies and resources, such as reliable antibodies against a protein of interest. Although targeted approaches provide some insight, here, we describe the workflow of two complementary mass spectrometry approaches, which provide a more comprehensive and non-biased analysis of the molecular features of the tissue of interest. Matrix-assisted laser desorption\/ionization (MALDI) mass spectrometry imaging (MSI) generates spatial intensity maps of molecular features, which can be easily correlated with histology. Additionally, liquid chromatography tandem mass spectrometry (LC-MS\/MS) can identify and quantify proteins of interest from a consecutive section of the same tissue. Here, we present data from concurrent precancerous lesions from the endometrium and fallopian tube of a single patient. Using this complementary approach, we monitored the abundance of hundreds of proteins within the precancerous and neighboring healthy regions. The method described here represents a useful tool to maximize the number of molecular data acquired from small sample sizes or even from a single case. Our initial data are indicative of a migratory phenotype in these lesions and warrant further research into their malignant capabilities.<\/jats:p>","DOI":"10.3390\/mps5040057","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T00:06:21Z","timestamp":1657497981000},"page":"57","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Protocol for the Acquisition of Comprehensive Proteomics Data from Single Cases Using Formalin-Fixed Paraffin Embedded Sections"],"prefix":"10.3390","volume":"5","author":[{"given":"Mitchell","family":"Acland","sequence":"first","affiliation":[{"name":"Adelaide Proteomics Centre, School of Biological Sciences, The University of Adelaide, Adelaide, SA 5005, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parul","family":"Mittal","sequence":"additional","affiliation":[{"name":"Clinical & Health Science, Mawson Lakes Campus, University of South Australia, Adelaide, SA 5095, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgia","family":"Arentz","sequence":"additional","affiliation":[{"name":"Adelaide Proteomics Centre, School of Biological Sciences, The University of Adelaide, Adelaide, SA 5005, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fergus","family":"Whitehead","sequence":"additional","affiliation":[{"name":"Clinpath Pathology, 21 James Congdon Drive, Mile End, Adelaide, SA 5031, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Clinical & Health Science, Mawson Lakes Campus, University of South Australia, Adelaide, SA 5095, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Klingler-Hoffmann","sequence":"additional","affiliation":[{"name":"Clinical & Health Science, Mawson Lakes Campus, University of South Australia, Adelaide, SA 5095, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin K.","family":"Oehler","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology, Royal Adelaide Hospital, North Terrace, Adelaide, SA 5000, Australia"},{"name":"Robinson Research Institute, Discipline of Obstetrics and Gynaecology, Adelaide Medical School, The University of Adelaide, Adelaide, SA 5000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"339695","DOI":"10.1016\/j.aca.2022.339695","article-title":"DIA-MS proteome analysis of formalin-fixed paraffin-embedded glioblastoma tissues","volume":"1204","author":"Weke","year":"2022","journal-title":"Anal. 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