{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T04:41:02Z","timestamp":1778474462140,"version":"3.51.4"},"reference-count":37,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T00:00:00Z","timestamp":1693785600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["21-74-00083"],"award-info":[{"award-number":["21-74-00083"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MPs"],"abstract":"<jats:p>The recently discovered Jingmenvirus group includes viruses with a segmented genome, RNA of a positive polarity, and several proteins with distant homology to the proteins of the members of the genus Orthoflavivirus. Some Jingmenvirus group members, namely the Alongshan virus (ALSV) and Jingmen tick virus, are reported to be tick-borne human pathogens that can cause a wide variety of symptoms. The ALSV is widely distributed in Eurasia, yet no reliable assay that can detect it exists. We describe a qPCR system for ALSV detection. Our data showed that this system can detect as little as 104 copies of the ALSV in a sample. The system showed no amplification of the common tick-borne viruses circulating in Eurasia, i.e., the Yanggou tick virus\u2014which is another Jingmenvirus group member\u2014or some known members of the genus Orthoflavivirus. The qPCR system was tested and had no nonspecific signal for the Ixodes ricinus, I. persulcatus, Dermacentor reticulatus, D. marginatus, Haemaphysalis concinna, and H. japonica ticks. The qPCR system had no nonspecific signal for human and sheep serum as well. Overall, the qPCR system described here can be used for reliable and quantitative ALSV detection.<\/jats:p>","DOI":"10.3390\/mps6050079","type":"journal-article","created":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T02:59:55Z","timestamp":1693796395000},"page":"79","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Quantitative Polymerase Chain Reaction System for Alongshan Virus Detection"],"prefix":"10.3390","volume":"6","author":[{"given":"Alexander G.","family":"Litov","sequence":"first","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI \u201cChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Egor V.","family":"Okhezin","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI \u201cChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"},{"name":"Department of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3764-7081","authenticated-orcid":false,"given":"Ivan S.","family":"Kholodilov","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI \u201cChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra E.","family":"Polienko","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI \u201cChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Galina G.","family":"Karganova","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI \u201cChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS\u201d (Institute of Poliomyelitis), 108819 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,4]]},"reference":[{"key":"ref_1","unstructured":"Knipe, D.M., and Howley, P.M. 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