{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T13:24:30Z","timestamp":1786368270871,"version":"build-2736575974"},"reference-count":16,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Walloon Region"},{"name":"University of Li\u00e8ge"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MPs"],"abstract":"<jats:p>The main causes of mortality in horses are the gastrointestinal pathologies associated with septic shock. Stem cells have shown, through systemic injection, a capacity to decrease inflammation and to regenerate injured tissue faster. Nevertheless, to achieve this rapid and total regeneration, systemic injections of 1 to 2 million cells per kilogram of body weight must be considered. Here, we demonstrate for the first time the feasibility and expansion capacity of equine muscle-derived mesenchymal stromal cells (mdMSCs) in a functionally closed, automated, perfusion-based, hollow-fiber bioreactor (HFBR) called the Quantum\u2122 Cell Expansion System (Terumo Blood and Cell Technologies). This feature greatly increases the number of generated cells with a surface area of 1.7 m2. The expansion of mdMSCs is very efficient in this bioreactor. The maximum expansion generated twenty times more cells than the initial seeding in nine days. The best returns were observed with an optimal seeding between 10 and 25 million mdMSCs, using the Bull\u2019s eye loading method and with a run duration between 7 and 10 days. Moreover, all the generated cells kept their stem properties: the ability to adhere to plastic and to differentiate into chondroblasts, osteoblasts and adipocytes. They also showed the expression of CD-44 and CD-90 markers, with a positive rate above 93%, while CD-45 and MHCII were non-expressed, with a positive rate below 0.5%. By capitalizing on the scalability, automation and 3D culture capabilities of the Quantum\u2122, it is possible to generate large quantities of high-quality equine mdMSCs for gastrointestinal disorders and other clinical applications.<\/jats:p>","DOI":"10.3390\/mps7020032","type":"journal-article","created":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T01:26:10Z","timestamp":1712021170000},"page":"32","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Optimization of the Amplification of Equine Muscle-Derived Mesenchymal Stromal Cells in a Hollow-Fiber Bioreactor"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1080-855X","authenticated-orcid":false,"given":"Julien","family":"Duysens","sequence":"first","affiliation":[{"name":"Revatis SA, Rue de la Science 8, 6900 Marche-En-Famenne, Belgium"},{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Graide","sequence":"additional","affiliation":[{"name":"Revatis SA, Rue de la Science 8, 6900 Marche-En-Famenne, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Niesten","sequence":"additional","affiliation":[{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9777-3023","authenticated-orcid":false,"given":"Ange","family":"Mouithys-Mickalad","sequence":"additional","affiliation":[{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justine","family":"Ceusters","sequence":"additional","affiliation":[{"name":"Revatis SA, Rue de la Science 8, 6900 Marche-En-Famenne, Belgium"},{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Serteyn","sequence":"additional","affiliation":[{"name":"Revatis SA, Rue de la Science 8, 6900 Marche-En-Famenne, Belgium"},{"name":"Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,2]]},"reference":[{"key":"ref_1","first-page":"42","article-title":"Gastrointestinal disorders and colic in the horse: Review of recent developments and future perspectives","volume":"177","author":"Tamzali","year":"2008","journal-title":"Vet. J."},{"key":"ref_2","unstructured":"Mair, T., and Divers, T. (2017). Equine Gastrointestinal Surgery, John Wiley & Sons."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1177\/10406387221081217","article-title":"Special section on diseases of the equine gastrointestinal tract","volume":"34","author":"Navarro","year":"2022","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.cveq.2014.04.001","article-title":"Evaluation of the colic in horses: Decision for referral","volume":"30","author":"Cook","year":"2014","journal-title":"Vet. Clin. N. Am. Equine Pract."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"105881","DOI":"10.1136\/vr.105881","article-title":"Indicators of \u2018critical\u2019 outcomes in 941 horses seen \u2018out-of-hours\u2019 for colic","volume":"187","author":"Bowden","year":"2020","journal-title":"Vet. Rec."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Blangy-Letheule, A., Vergnaud, A., Dupas, T., Rozec, B., Lauzier, B., and Leroux, A.A. (2023). Spontaneous Sepsis in Adult Horses: From Veterinary to Human Medicine Perspectives. Cells, 12.","DOI":"10.3390\/cells12071052"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1002\/cyto.a.22402","article-title":"Human adult stem cells from diverse origins: An overview from multiparametric immunophenotyping to clinical applications","volume":"85","author":"Sousa","year":"2014","journal-title":"Cytom. Part A"},{"key":"ref_8","first-page":"8","article-title":"Mesenchymal stromal cells Beyond Regenerative Medicine","volume":"18","author":"Shammaa","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s12015-019-09932-0","article-title":"Therapeutic mesenchymal stromal stem cells: Isolation, characterization and role in equine regenerative medicine and metabolic disorders","volume":"16","author":"Bourebaba","year":"2020","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/j.cveq.2023.07.004","article-title":"Use of Biologics and Stem Cells in the Treatment of Other Inflammatory Diseases in the Horse","volume":"39","author":"Barrett","year":"2023","journal-title":"Vet. Clin. N. Am. Equine Pract."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.3727\/096368912X657990","article-title":"GMP-compliant isolation and expansion of bone marrow-derived MSCs in the closed, automated device quantum cell expansion system","volume":"22","author":"Rojewski","year":"2013","journal-title":"Cell Transplant."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/j.jcyt.2016.05.013","article-title":"Large-scale progenitor cell expansion for multiple donors in a monitored hollow fibre bioreactor","volume":"18","author":"Lambrechts","year":"2016","journal-title":"Cytotherapy"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2374","DOI":"10.1111\/trf.14805","article-title":"Characterization and cost-benefit analysis of automated bioreactor-expanded mesenchymal stromal cells for clinical applications","volume":"58","author":"Russell","year":"2018","journal-title":"Transfusion"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1038\/s41598-017-00803-7","article-title":"From skeletal muscle to stem cells: An innovative and minimally-invasive process for multiple species","volume":"7","author":"Ceusters","year":"2017","journal-title":"Sci. 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