{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:02:56Z","timestamp":1792036976698,"version":"4.4.0"},"reference-count":98,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T00:00:00Z","timestamp":1582243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003451","name":"Euskal Herriko Unibertsitatea","doi-asserted-by":"publisher","award":["UFI11\/56 and UFI11\/25"],"award-info":[{"award-number":["UFI11\/56 and UFI11\/25"]}],"id":[{"id":"10.13039\/501100003451","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003086","name":"Gobierno Vasco","doi-asserted-by":"publisher","award":["SAIOTEK S-PE13UN004"],"award-info":[{"award-number":["SAIOTEK S-PE13UN004"]}],"id":[{"id":"10.13039\/501100003086","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>Drug resistance of pathogenic microorganisms has become a global public health problem, which has prompted the development of new materials with antimicrobial properties. In this context, antimicrobial nanohybrids are an alternative due to their synergistic properties. In this study, we used an environmentally friendly one-step approach to synthesize graphene oxide (GO) decorated with silver nanoparticles (GO\u2013AgNPs). By this process, spherical AgNPs of average size less than 4 nm homogeneously distributed on the surface of the partially reduced GO can be generated in the absence of any stabilizing agent, only with ascorbic acid (L-AA) as a reducing agent and AgNO3 as a metal precursor. The size of the AgNPs can be controlled by the AgNO3 concentration and temperature. Smaller AgNPs are obtained at lower concentrations of the silver precursor and lower temperatures. The antimicrobial properties of nanohybrids against Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, Gram-positive Staphylococcus aureus, and the yeast Candida albicans were found to be concentration- and time-dependent. C. albicans and S. aureus showed the highest susceptibility to GO\u2013AgNPs. These nanohybrids can be used as nanofillers in polymer nanocomposites to develop materials with antimicrobial activity for applications in different areas, and another potential application could be cancer therapeutic agents.<\/jats:p>","DOI":"10.3390\/nano10020376","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T08:59:47Z","timestamp":1582275587000},"page":"376","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":202,"title":["Graphene Oxide\u2013Silver Nanoparticle Nanohybrids: Synthesis, Characterization, and Antimicrobial Properties"],"prefix":"10.3390","volume":"10","author":[{"given":"M\u00f3nica","family":"Cobos","sequence":"first","affiliation":[{"name":"Department of Polymer Science and Technology, Faculty of Chemistry, University of the Basque Country (UPV\/EHU), Paseo Manuel Lardiz\u00e1bal 3, 20018 San Sebasti\u00e1n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iker","family":"De-La-Pinta","sequence":"additional","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Medicine and Nursing, University of the Basque Country (UPV\/EHU), Barrio Sarriena s\/n, Leioa 48940, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0438-2248","authenticated-orcid":false,"given":"Guillermo","family":"Quind\u00f3s","sequence":"additional","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Medicine and Nursing, University of the Basque Country (UPV\/EHU), Barrio Sarriena s\/n, Leioa 48940, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Jes\u00fas","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Polymer Science and Technology, Faculty of Chemistry, University of the Basque Country (UPV\/EHU), Paseo Manuel Lardiz\u00e1bal 3, 20018 San Sebasti\u00e1n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6668-8192","authenticated-orcid":false,"given":"M. Dolores","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Polymer Science and Technology, Faculty of Chemistry, University of the Basque Country (UPV\/EHU), Paseo Manuel Lardiz\u00e1bal 3, 20018 San Sebasti\u00e1n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cis.2012.12.001","article-title":"Insights into biogenic and chemical production of inorganic nanomaterials and nanostructures","volume":"189\u2212190","author":"Faramarzi","year":"2013","journal-title":"Adv. Colloid Interfac."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"7278","DOI":"10.3390\/ma8115377","article-title":"Green synthesis of metallic nanoparticles via biological entities","volume":"8","author":"Shah","year":"2015","journal-title":"Materials"},{"key":"ref_3","first-page":"385","article-title":"Synthesis of silver nanoparticles: Chemical, physical and biological methods","volume":"9","author":"Iravani","year":"2014","journal-title":"Res. Pharm. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.tibtech.2013.01.003","article-title":"The greener synthesis of nanoparticles","volume":"31","author":"Kharissova","year":"2013","journal-title":"Trends Biotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Scala, A., Piperno, A., Hada, A., Astilean, S., Vulpoi, A., Ginestra, G., Marino, A., Nostro, A., Zammuto, V., and Gugliandolo, C. (2019). Marine bacterial exopolymers-mediated green synthesis of noble metal nanoparticles with antimicrobial properties. Polymers, 11.","DOI":"10.3390\/polym11071157"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"N\u00fa\u00f1ez, Y.A.R., Castro, R.I., Arenas, F.A., Lopez-Caba\u00f1a, Z.E., Carre\u00f1o, G., Carrasco-Sanchez, V., Marican, A., Villase\u00f1or, J., Vargas, E., and Santos, L.S. (2019). Preparation of hydrogel\/silver nanohybrids mediated by tunable-size silver nanoparticles for potential antibacterial applications. Polymers, 11.","DOI":"10.3390\/polym11040716"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.1128\/AEM.02218-06","article-title":"Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli","volume":"73","author":"Pal","year":"2007","journal-title":"Appl. Environ. Microb."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev-bioeng-071811-150124","article-title":"The effect of nanoparticle size, shape, and surface chemistry on biological systems","volume":"14","author":"Albanese","year":"2012","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Raza, M.A., Kanwal, Z., Rauf, A., Sabri, A.N., Riaz, S., and Naseem, S. (2016). Size- and shape-dependent antibacterial studies of silver nanoparticles synthesized by wet chemical routes. Nanomaterials, 6.","DOI":"10.3390\/nano6040074"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2346","DOI":"10.1088\/0957-4484\/16\/10\/059","article-title":"The bactericidal effect of silver nanoparticles","volume":"16","author":"Morones","year":"2005","journal-title":"Nanotechnology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.jcis.2011.05.009","article-title":"Synthesis and characterization of silver nanoparticle and graphene oxide nanosheet composites as a bactericidal agent for water disinfection","volume":"360","author":"Bao","year":"2011","journal-title":"J. Colloid Interf. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.colsurfb.2010.10.033","article-title":"Synthesis of silver nanoparticles in an aqueous suspension of graphene oxide sheets and its antimicrobial activity","volume":"83","author":"Das","year":"2011","journal-title":"Colloid Surf. B"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.colsurfb.2012.12.033","article-title":"The synthesis of citrate-modified silver nanoparticles in an aqueous suspension of graphene oxide nanosheets and their antibacterial activity","volume":"105","author":"Das","year":"2013","journal-title":"Colloids Surf. B"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s12274-010-1037-x","article-title":"Facile synthesis and application of Ag-chemically converted graphene nanocomposite","volume":"3","author":"Shen","year":"2010","journal-title":"Nano Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1186\/1556-276X-7-541","article-title":"Antibacterial performance of Ag nanoparticles and AgGO nanocomposites prepared via rapid microwave-assisted synthesis method","volume":"7","author":"Chook","year":"2012","journal-title":"Nanoscale Res. Lett."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3350","DOI":"10.1039\/C0JM02806A","article-title":"Preparation, characterization and antibacterial properties of silver-modified graphene oxide","volume":"21","author":"Ma","year":"2011","journal-title":"J. Mater. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3867","DOI":"10.1021\/am4005495","article-title":"Graphene oxide\u2212silver nanocomposite as a highly effective antibacterial agent with species-specific mechanisms","volume":"5","author":"Tang","year":"2013","journal-title":"ACS Appl. Mater. Inter. ACS Appl. Mater. Inter."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.colsurfb.2013.08.006","article-title":"Anti-adhesion and antibacterial activity of silver nanoparticles supported on graphene oxide sheets","volume":"113","author":"Martinez","year":"2014","journal-title":"Colloids Surf. B"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1016\/j.apsusc.2012.08.094","article-title":"Green synthesis of graphene\/Ag nanocomposites","volume":"261","author":"Yuan","year":"2012","journal-title":"App. Surf. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3407","DOI":"10.1016\/j.carbon.2012.02.002","article-title":"The use of polyethyleneimine-modified reduced graphene oxide as a substrate for silver nanoparticles to produce a material with lower cytotoxicity and long-term antibacterial activity","volume":"50","author":"Cai","year":"2012","journal-title":"Carbon"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1002\/smll.200900726","article-title":"A facile and novel synthesis of Ag\u2013graphene-based nanocomposites","volume":"5","author":"Pasricha","year":"2009","journal-title":"Small"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.actamat.2013.10.045","article-title":"Green synthesis of dimension-controlled silver nanoparticle-graphene oxide with in situ ultrasonication","volume":"64","author":"Hui","year":"2014","journal-title":"Acta Mater."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"7795","DOI":"10.1039\/c1jm10671f","article-title":"One-pot hydrothermal synthesis of Ag-reduced graphene oxide composite with ionic liquid","volume":"21","author":"Shen","year":"2011","journal-title":"J. Mater. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"8433","DOI":"10.1021\/la401662d","article-title":"Fast Process to decorate silver nanoparticles on carbon nanomaterials for preparing high-performance flexible transparent conductive films","volume":"29","author":"Li","year":"2013","journal-title":"Langmuir"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"7295","DOI":"10.1039\/C3TA15307J","article-title":"Highly efficient silver-assisted reduction of graphene oxide dispersions at room temperature: Mechanism, and catalytic and electrochemical performance of the resulting hybrids","volume":"2","author":"Paredes","year":"2014","journal-title":"J. Mater. Chem. A"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ccr.2015.12.005","article-title":"Metal nanoparticles supported on two-dimensional graphenes as heterogeneous catalysts","volume":"312","author":"Navalon","year":"2016","journal-title":"Coordin. Chem. Rev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.bios.2012.10.029","article-title":"Green synthesis of silver nanoparticles\u2013graphene oxidenanocomposite and its application in electrochemical sensing of tryptophan","volume":"42","author":"Li","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2748","DOI":"10.1021\/acsanm.8b00444","article-title":"Surfactant-free synthesis of graphene oxide coated silver nanoparticles for SERS biosensing and intracellular drug delivery","volume":"1","author":"Zeng","year":"2018","journal-title":"ACS Appl. Nano Mater."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Vi, T.T.T., Kumar, S.R., Rout, B., Liu, C., Wong, C., Chang, C., Chen, C., Chen, D.W., and Lue, S.J. (2018). The preparation of graphene oxide-silver nanocomposites: The effect of silver loads on Gram-positive and Gram-negative antibacterial activities. Nanomaterials, 8.","DOI":"10.3390\/nano8030163"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"282001","DOI":"10.1088\/1361-6528\/aabbff","article-title":"Nanocomposites: Suitable alternatives as antimicrobial agents","volume":"29","author":"Matharu","year":"2018","journal-title":"Nanotechnology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5603","DOI":"10.1016\/j.polymer.2008.09.014","article-title":"Use of electrospinning technique for biomedical applications","volume":"49","author":"Agarwal","year":"2008","journal-title":"Polymer"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"041401","DOI":"10.1063\/1.5110965","article-title":"Experimental and theoretical investigation of the fluid behavior during polymeric fiber formation with and without pressure","volume":"6","author":"Alenezi","year":"2019","journal-title":"Appl. Phys. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"20170058","DOI":"10.1098\/rsfs.2017.0058","article-title":"The effect of graphene-poly(methyl methacrylate) fibres on microbial growth","volume":"8","author":"Matharu","year":"2018","journal-title":"Interface Focus"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Burdusel, A.C., Gherasim, O., Grumezescu, A.M., Mogoanta, L., Ficai, A., and Andronescu, E. (2018). Biomedical applications of silver nanoparticles: An up-to-date overview. Nanomaterials, 8.","DOI":"10.3390\/nano8090681"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"45092","DOI":"10.1002\/app.45092","article-title":"Chitosan\u2013graphene oxide nanocomposites: Effect of graphene oxide nanosheets and glycerol plasticizer on thermal and mechanical properties","volume":"134","author":"Cobos","year":"2017","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1002\/cphc.200500113","article-title":"Synthesis and optical properties of silver nanoparticles and arrays","volume":"6","author":"Evanoff","year":"2005","journal-title":"Chem. Phys. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1002\/ejic.200901048","article-title":"Ag\/graphene heterostructures: Synthesis, characterization and optical properties","volume":"8","author":"Li","year":"2010","journal-title":"Eur. J. Inorg. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.colsurfb.2010.05.024","article-title":"Bioprospective of Sorbus aucuparia leaf extract in development of silver and gold nanocolloids","volume":"80","author":"Dubey","year":"2010","journal-title":"Colloids Surf. B"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Huang, L., Yang, H., Zhang, Y., and Xiao, W. (2016). Study on synthesis and antibacterial properties of AgNPs\/GO nanocomposites. J. Nanomater., 5685967.","DOI":"10.1155\/2016\/5685967"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4742","DOI":"10.1021\/ic300332x","article-title":"Environmentally friendly, one-pot synthesis of ag nanoparticle decorated reduced graphene oxide composites and their application to photocurrent generation","volume":"51","author":"Tian","year":"2012","journal-title":"Inorg. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Jiang, X.C., Chen, W.M., Chen, C.Y., Xiong, S.X., and Yu, A.B. (2011). Role of temperature in the growth of silver nanoparticles through a synergetic reduction approach. Nanoscale Res. Lett., 6.","DOI":"10.1007\/s11671-010-9780-1"},{"key":"ref_42","first-page":"1366","article-title":"Effect of temperature on the size of biosynthesized silver nanoparticle: Deep insight into microscopic kinetics analysis","volume":"23","author":"Liu","year":"2017","journal-title":"Arab. J. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.2147\/IDR.S177247","article-title":"Health care-associated infections\u2014An overview","volume":"11","author":"Haque","year":"2018","journal-title":"Infect. Drug. Resist."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s10123-018-0014-1","article-title":"The continuous changes in the aetiology and epidemiology of invasive candidiasis: From the familiar Candida albicans to multiresistant Candida Auris","volume":"21","author":"Mateo","year":"2018","journal-title":"Int. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Kramer, A., Schwebke, I., and Kampf, G. (2006). How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC. Infect. Dis., 6.","DOI":"10.1186\/1471-2334-6-130"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1111\/j.1469-0691.2012.03903.x","article-title":"The changing epidemiology of Staphylococcus aureus bloodstream infection: A multinational population-based surveillance study","volume":"19","author":"Laupland","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s13756-014-0039-y","article-title":"Temporal trends and risk factors for extended-spectrum beta-lactamase-producing Escherichia coli in adults with catheter-associated urinary tract infections","volume":"3","author":"Spadafino","year":"2014","journal-title":"Antimicrob Resist Infect Control"},{"key":"ref_48","first-page":"332","article-title":"Pseudomonas aeruginosa infections in patients, hospital means, and personnel\u2019s specimens","volume":"17","author":"Fazeli","year":"2012","journal-title":"J. Res. Med. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1086\/421946","article-title":"Nosocomial bloodstream infections in US hospitals: Analysis of 24,179 cases from a prospective nationwide surveillance study","volume":"39","author":"Wisplinghoff","year":"2004","journal-title":"Clin. Infect. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"8100","DOI":"10.1021\/nn202699t","article-title":"Graphene oxide: A nonspecific enhancer of cellular growth","volume":"5","author":"Ruiz","year":"2011","journal-title":"ACS Nano"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"165101","DOI":"10.1088\/0957-4484\/25\/16\/165101","article-title":"Graphene oxide as an anaerobic membrane scaffold for the enhancement of B. adolescentis proliferation and antagonistic effects against pathogens E. coli and S. aureus","volume":"25","author":"Chen","year":"2014","journal-title":"Nanotechnology"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"6971","DOI":"10.1021\/nn202451x","article-title":"Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: Membrane and oxidative stress","volume":"5","author":"Liu","year":"2011","journal-title":"ACS Nano"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"4317","DOI":"10.1021\/nn101097v","article-title":"Graphene-based antibacterial paper","volume":"4","author":"Hu","year":"2010","journal-title":"ACS Nano"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"7226","DOI":"10.1021\/acsnano.5b02067","article-title":"Antimicrobial properties of graphene oxide nanosheets: Why size matters","volume":"9","author":"Perreault","year":"2015","journal-title":"ACS Nano"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"12364","DOI":"10.1021\/la3023908","article-title":"Lateral dimension-dependent antibacterial activity of graphene oxide sheets","volume":"28","author":"Liu","year":"2012","journal-title":"Langmuir"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"996","DOI":"10.4315\/0362-028X.JFP-14-463","article-title":"Toxicity of graphene oxide on intestinal bacteria and caco-2 cells","volume":"78","author":"Nguyen","year":"2015","journal-title":"J. Food Protect."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"3882","DOI":"10.1016\/j.biomaterials.2013.02.001","article-title":"The antifungal activity of graphene oxide-silver nanocomposites","volume":"34","author":"Li","year":"2013","journal-title":"Biomaterials"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"230","DOI":"10.3844\/ojbsci.2014.230.239","article-title":"Graphene oxide as antimicrobial against two Gram-positive and two Gram-negative bacteria in addition to one fungus","volume":"14","author":"Patan","year":"2014","journal-title":"OnLine J. Biol. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.1021\/jacs.5b11411","article-title":"Mechanisms of the antimicrobial activities of graphene materials","volume":"138","author":"Zou","year":"2016","journal-title":"J. Am. Chem. Soc."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"6279","DOI":"10.1021\/jp200686k","article-title":"Wrapping bacteria by graphene nanosheets for isolation from environment, reactivation by sonication, and inactivation by near-infrared irradiation","volume":"115","author":"Akhavan","year":"2011","journal-title":"J. Phys. Chem. B"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"5901","DOI":"10.2147\/IJN.S37397","article-title":"Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa","volume":"7","author":"Gurunathan","year":"2012","journal-title":"Int. J. Nanomed."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"13183","DOI":"10.1021\/am503070z","article-title":"Availability of the basal planes of graphene oxide determines whether it is antibacterial","volume":"6","author":"Hui","year":"2014","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Gusev, A., Zakharova, O., Muratov, D.S., Vorobeva, N.S., Sarker, M., Rybkin, I., Bratashov, D., Kolesnikov, E., Lapanje, A., and Kuznetsov, D.V. (2019). Medium-dependent antibacterial properties and bacterial filtration ability of reduced graphene oxide. Nanomaterials, 9.","DOI":"10.3390\/nano9101454"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"544","DOI":"10.3389\/fmicb.2014.00544","article-title":"The importance of growth kinetic analysis in determining bacterial susceptibility against antibiotics and silver nanoparticles","volume":"5","author":"Theophel","year":"2014","journal-title":"Front. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"045007","DOI":"10.1088\/2053-1591\/1\/4\/045007","article-title":"Facile fabrication of graphene oxide loaded with silver nanoparticles as antifungal materials","volume":"1","author":"Cui","year":"2014","journal-title":"Mater. Res. Express"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Jaworski, S., Wierzbicki, M., Sawosz, E., Jung, A., Gielerak, G., Biernat, J., Jaremek, H., \u0141ojkowski, W., Wo\u017aniak, B., and Wojnarowicz, J. (2018). Graphene oxide-based nanocomposites decorated with silver nanoparticles as an antibacterial agent. Nanoscale Res. Lett., 13.","DOI":"10.1186\/s11671-018-2533-2"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"6966","DOI":"10.1021\/acsami.5b00937","article-title":"Preparation, characterization, and antibacterial activity of silver nanoparticle-decorated graphene oxide nanocomposite","volume":"7","author":"Shao","year":"2015","journal-title":"ACS Appl. Mater. Inter."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1002\/elsc.201700138","article-title":"Graphene oxide\/silver nanohybrid: Optimization, antibacterial activity and its impregnation on bacterial cellulose as a potential wound dressing based on GO\u2013Ag nanocomposite-coated BC","volume":"18","author":"Mohammadnejad","year":"2018","journal-title":"Eng. Life Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1007\/s10856-013-4894-5","article-title":"Size-dependent antibacterial activities of silver nanoparticles against oral anaerobic pathogenic bacteria","volume":"24","author":"Lu","year":"2013","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.2147\/IJN.S191340","article-title":"Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa","volume":"14","author":"Liao","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"16248","DOI":"10.1021\/jp063826h","article-title":"Silver colloid nanoparticles: Synthesis, characterization, and their antibacterial activity","volume":"110","author":"Panacek","year":"2006","journal-title":"J. Phys. Chem. B"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1007\/s11051-008-9428-6","article-title":"Synthesis and antibacterial activity of silver nanoparticles with different sizes","volume":"10","author":"Ruiz","year":"2008","journal-title":"J. Nanopart. Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.talanta.2013.09.017","article-title":"Preparation of graphene oxide-silver nanoparticle nanohybrids with highly antibacterial capability","volume":"117","author":"Zhu","year":"2013","journal-title":"Talanta"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"18323","DOI":"10.1039\/c3ra41731j","article-title":"Developing green photochemical approaches towards the synthesis of carbon nanofiber-and graphene-supported silver nanoparticles and their use in the catalytic reduction of 4-nitrophenol","volume":"3","author":"Guardia","year":"2013","journal-title":"RSC Adv."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1021\/pr0504079","article-title":"Proteomic analysis of the mode of antibacterial action of silver nanoparticles","volume":"5","author":"Lok","year":"2006","journal-title":"J. Proteome Res."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"5649","DOI":"10.1021\/es101072s","article-title":"Antibacterial activity of nanosilver ions and particles","volume":"44","author":"Sotiriou","year":"2010","journal-title":"Environ. Sci. Technol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"244","DOI":"10.3109\/17435390.2010.525669","article-title":"Antibacterial efficacy of silver nanoparticles of different sizes, surface conditions and synthesis methods","volume":"5","author":"Samberg","year":"2011","journal-title":"Nanotoxicology"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.jcis.2004.02.012","article-title":"Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria","volume":"275","author":"Sondi","year":"2004","journal-title":"J. Colloid Interf. Sci."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"3974","DOI":"10.1039\/C3RA44507K","article-title":"Size-controlled silver nanoparticles synthesized over the range 5\u2212100 nm using the same protocol and their antibacterial efficacy","volume":"4","author":"Agnihotri","year":"2014","journal-title":"RSC Adv."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1166\/jnn.2005.034","article-title":"Synthesis and antibacterial properties of silver nanoparticles","volume":"5","author":"Baker","year":"2005","journal-title":", J. Nanosci. Nanotechnol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"4583","DOI":"10.1021\/es703238h","article-title":"Size dependent and reactive oxygen species related nanosilver toxicity to nitrifying bacteria","volume":"42","author":"Choi","year":"2008","journal-title":"Environ. Sci. Technol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"4271","DOI":"10.1021\/nl301934w","article-title":"Negligible particle-specific antibacterial activity of silver nanoparticles","volume":"12","author":"Xiu","year":"2012","journal-title":"Nano Lett."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Kim, M., Jee, S.-C., Shinde, S.K., Mistry, B.M., Saratale, R.G., Saratale, G.D., Ghodake, G.S., Kim, D.-Y., Sung, J.-S., and Kadam, A.A. (2019). Green-synthesis of anisotropic peptone-silver nanoparticles and its potential application as anti-bacterial agent. Polymers, 11.","DOI":"10.3390\/polym11020271"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s10856-019-6281-3","article-title":"Effect of biomaterials hydrophobicity and roughness on biofilm development","volume":"30","author":"Cobos","year":"2019","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.carbon.2019.05.011","article-title":"One-step eco-friendly synthesized silver-graphene oxide\/poly(vinylalcohol) antibacterial nanocomposites","volume":"150","author":"Cobos","year":"2019","journal-title":"Carbon"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jare.2017.10.008","article-title":"A systematic review on silver nanoparticles-induced cytotoxicity: Physicochemical properties and perspectives","volume":"9","author":"Akter","year":"2018","journal-title":"J. Adv. Res."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s12951-016-0165-1","article-title":"Comparative in vitro toxicity of a graphene oxide-silver nanocomposite and the pristine counterparts toward macrophages","volume":"14","author":"Consonni","year":"2016","journal-title":"J. Nanobiotechnol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1186\/1556-276X-9-459","article-title":"Oxidative stress mediated cytotoxicity of biologically synthesized silver nanoparticles in human lung epithelial adenocarcinoma cell line","volume":"9","author":"Han","year":"2014","journal-title":"Nanoscale Res. Lett."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1002\/jat.3199","article-title":"Cytotoxicity and apoptosis induced by silver nanoparticles in human liver HepG2 cells in different dispersion media","volume":"36","author":"Xue","year":"2015","journal-title":"J. Appl. Toxicol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1002\/jbm.a.34053","article-title":"Size-dependent cellular toxicity of silver nanoparticles","volume":"100","author":"Kim","year":"2012","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"012025","DOI":"10.1088\/1742-6596\/429\/1\/012025","article-title":"Comparison of toxicity of uncoated and coated silver nanoparticles","volume":"429","author":"Nguyen","year":"2013","journal-title":"J. Phys. Conf. Ser."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Cameron, S.J., Hosseinian, F., and Willmore, W.G. (2018). A Current Overview of the Biological and Cellular Effects of Nanosilver. Int. J. Mol. Sci., 12.","DOI":"10.3390\/ijms19072030"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"9810","DOI":"10.1016\/j.biomaterials.2011.08.085","article-title":"The effect of particle size on the cytotoxicity, inflammation, developmental toxicity and genotoxicity of silver nanoparticles","volume":"32","author":"Park","year":"2011","journal-title":"Biomaterials"},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"De Matteis, V., Cascione, M., Toma, C.C., and Leporatti, S. (2018). Silver Nanoparticles: Synthetic Routes, In vitro toxicity and theranostic applications for cancer disease. Nanomaterials, 8.","DOI":"10.3390\/nano8050319"},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Gurunathan, S., Kang, M.-H., Qasim, M., and Kim, J.-H. (2018). Nanoparticle-Mediated Combination Therapy: Two-in-One Approach for Cancer. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19103264"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1016\/j.jcis.2017.07.002","article-title":"Anticancer activity of graphene oxide- reduced graphene oxide- silver nanoparticle composites","volume":"505","author":"Kavinkumar","year":"2017","journal-title":"J. Colloid Interface Sci."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1021\/nn800596w","article-title":"Cytotoxicity and genotoxicity of silver nanoparticles in human cells","volume":"3","author":"AshaRani","year":"2009","journal-title":"ACS Nano"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Choi, Y.J., Gurunathan, S., and Kim, J.H. (2018). Graphene oxide-silver nanocomposite enhances cytotoxic and apoptotic potential of salinomycin in human ovarian cancer stem cells (OvCSCs): A novel approach for cancer therapy. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19030710"}],"container-title":["Nanomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4991\/10\/2\/376\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:59:39Z","timestamp":1760173179000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4991\/10\/2\/376"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,21]]},"references-count":98,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["nano10020376"],"URL":"https:\/\/doi.org\/10.3390\/nano10020376","relation":{},"ISSN":["2079-4991"],"issn-type":[{"value":"2079-4991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,21]]}}}