{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:48:48Z","timestamp":1789580928808,"version":"build-2803163510"},"reference-count":71,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,17]],"date-time":"2020-04-17T00:00:00Z","timestamp":1587081600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006435","name":"Central European Institute of Technology","doi-asserted-by":"publisher","award":["CEITEC 2020 (LQ1601)"],"award-info":[{"award-number":["CEITEC 2020 (LQ1601)"]}],"id":[{"id":"10.13039\/501100006435","id-type":"DOI","asserted-by":"publisher"}]},{"name":"EFRR project \u201cMultidisciplinary research to increase application potential of nanomaterials in agricultural practice\u201d","award":["(No. CZ.02.1.01\/0.0\/0.0\/16_025\/0007314)"],"award-info":[{"award-number":["(No. CZ.02.1.01\/0.0\/0.0\/16_025\/0007314)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>In this study, the titanium\u2013gadolinium quantum dots (TGQDs) were novel, first of its type to be synthesized, and fully characterized to date. Multiple physical characterization includes scanning electron microscopy (SEM), scanning electrochemical microscope (SCEM), x-ray fluorescence, spectrophotometry, and dynamic light scattering were carried out. The obtained results confirmed appropriate size and shape distributions in addition to processing optical features with high quantum yield. The synthesized TGQD was used as a fluorescent dye for bacterial detection and imaging by fluorescent microscopy and spectrophotometry, where TGQD stained only bacterial cells, but not human cells. The significant antibacterial activities of the TGQDs were found against a highly pathogenic bacterium (Staphylococcus aureus) and its antibiotic resistant strains (vancomycin and methicillin resistant Staphylococcus aureus) using growth curve analysis and determination of minimum inhibitory concentration (MIC) analysis. Live\/dead cell imaging assay using phase-contrast microscope was performed for further confirmation of the antibacterial activity. Cell wall disruption and release of cell content was observed to be the prime mode of action with the reduction of cellular oxygen demand (OD).<\/jats:p>","DOI":"10.3390\/nano10040778","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T03:23:06Z","timestamp":1587439386000},"page":"778","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A Novel Biocompatible Titanium\u2013Gadolinium Quantum Dot as a Bacterial Detecting Agent with High Antibacterial Activity"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1175-7194","authenticated-orcid":false,"given":"Vishma Pratap","family":"Sur","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1\/1665, CZ-61300 Brno, Czech Republic"},{"name":"Central European Institute of Technology (CEITEC), Brno University 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The WHO priority list of antibiotic-resistant bacteria and tuberculosis","volume":"18","author":"Tacconelli","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1093\/femsre\/fux007","article-title":"Antibiotic resistance in Staphylococcus aureus. Current status and future prospects","volume":"41","author":"Foster","year":"2017","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Barker, A.K., Brown, K., Ahsan, M., Sengupta, S., and Safdar, N. (2017). Social determinants of antibiotic misuse: A qualitative study of community members in Haryana, India. BMC Public Health, 17.","DOI":"10.1186\/s12889-017-4261-4"},{"key":"ref_4","unstructured":"(2020, April 16). Antibiotics: Are You Misusing Them?. Available online: https:\/\/www.mayoclinic.org\/healthy-lifestyle\/consumer-health\/in-depth\/antibiotics\/art-20045720."},{"key":"ref_5","unstructured":"NHS U. (2020, April 16). Side Effects of Antibiotics. Available online: https:\/\/www.nhs.uk\/conditions\/antibiotics\/side-effects\/."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1093\/jac\/33.3.387","article-title":"Hepatic side-effects of antibiotics","volume":"33","author":"Westphal","year":"1994","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1046\/j.1464-5491.2003.00860.x","article-title":"Methicillin-resistant Staphylococcus aureus in the diabetic foot clinic: A worsening problem","volume":"20","author":"Dang","year":"2003","journal-title":"Diabet. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.3389\/fmicb.2018.01441","article-title":"Nano-Strategies to Fight Multidrug Resistant Bacteria-\u201cA Battle of the Titans\u201d","volume":"9","author":"Baptista","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.apmt.2015.10.001","article-title":"Synergistic antibacterial activity of PEGylated silver\u2013graphene quantum dots nanocomposites","volume":"1","author":"Habiba","year":"2015","journal-title":"Appl. Mater. Today"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/j.arcmed.2005.06.009","article-title":"Resistance to Antibiotics: Are We in the Post-Antibiotic Era?","volume":"36","author":"Alanis","year":"2005","journal-title":"Arch. Med. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1017\/S1466252308001497","article-title":"Antimicrobial peptides and bacteriocins: Alternatives to traditional antibiotics","volume":"9","author":"Sang","year":"2008","journal-title":"Anim. Health Res. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Rai, M.K., and Kon, K.V. (2013). Chapter 1\u2014Alternative Antimicrobial Approaches to Fighting Multidrug-Resistant Infections. Fighting Multidrug Resistance with Herbal Extracts, Essential Oils and Their Components, Academic Press.","DOI":"10.1016\/B978-0-12-398539-2.00001-X"},{"key":"ref_13","unstructured":"Rai, M.K., and Kon, K.V. (2013). Chapter 3\u2014Bioactivity of Plant Constituents against Vancomycin-Resistant Enterococci. Fighting Multidrug Resistance with Herbal Extracts, Essential Oils and Their Components, Academic Press."},{"key":"ref_14","unstructured":"Rai, M.K., and Kon, K.V. (2013). Chapter 2\u2014Natural Plant Products Used against Methicillin-Resistant Staphylococcus aureus. Fighting Multidrug Resistance with Herbal Extracts, Essential Oils and Their Components, Academic Press."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Barros, C.H.N., Fulaz, S., Stanisic, D., and Tasic, L. (2018). Biogenic Nanosilver against Multidrug-Resistant Bacteria (MDRB). Antibiotics, 7.","DOI":"10.3390\/antibiotics7030069"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4485","DOI":"10.1038\/s41598-019-41005-7","article-title":"A Peptide-Nanoparticle System with Improved Efficacy against Multidrug Resistant Bacteria","volume":"9","author":"Pal","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"8211","DOI":"10.2147\/IJN.S132163","article-title":"Nanomaterials for alternative antibacterial therapy","volume":"12","author":"Hemeg","year":"2017","journal-title":"Int. J. Nanomed."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s12951-018-0392-8","article-title":"Nano based drug delivery systems: Recent developments and future prospects","volume":"16","author":"Patra","year":"2018","journal-title":"J. Nanobiotechnol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Sur, V.P., Kominkova, M., Buchtova, Z., Dolezelikova, K., Zitka, O., and Moulick, A. (2019). CdSe QD Biosynthesis in Yeast Using Tryptone-Enriched Media and Their Conjugation with a Peptide Hecate for Bacterial Detection and Killing. Nanomaterials, 9.","DOI":"10.3390\/nano9101463"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1007\/s11671-007-9081-5","article-title":"Nanoparticles for applications in cellular imaging","volume":"2","author":"Thurn","year":"2007","journal-title":"Nanoscale Res. Lett."},{"key":"ref_21","first-page":"1","article-title":"Toxicity of Nanoparticles and an Overview of Current Experimental Models","volume":"20","author":"Bahadar","year":"2016","journal-title":"Iran. Biomed. J."},{"key":"ref_22","unstructured":"Grumezescu, A.M. (2017). Chapter 18\u2014Toxicity of Nanoparticles: Etiology and Mechanisms. Antimicrobial Nanoarchitectonics, Elsevier."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1002\/cyto.a.20069","article-title":"Mechanism and use of the commercially available viability stain, BacLight","volume":"61","author":"Stocks","year":"2004","journal-title":"Cytom. Part A"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1093\/cid\/ciu1153","article-title":"Clinical Evaluation of Tuberculosis Viability Microscopy for Assessing Treatment Response","volume":"60","author":"Datta","year":"2014","journal-title":"Clin. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.ijmyco.2016.06.003","article-title":"Fluorescein diacetate vital staining for detecting viability of acid-fast bacilli in patients on antituberculosis treatment","volume":"5","author":"Kanade","year":"2016","journal-title":"Int. J. Mycobacteriol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1093\/jac\/dks379","article-title":"The enhanced permeability retention effect: A new paradigm for drug targeting in infection","volume":"68","author":"Azzopardi","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Anderson, D., and Mj, S. (2016). Nanotechnology: The Risks and Benefits for Medical Diagnosis and Treatment. J. Nanomed. Nanotechnol., 7.","DOI":"10.4172\/2157-7439.1000e143"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"35859","DOI":"10.1021\/acsami.8b15868","article-title":"Real-Time Visualization of Cell Membrane Damage Using Gadolinium\u2013Schiff Base Complex-Doped Quantum Dots","volume":"10","author":"Moulick","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4428","DOI":"10.1016\/j.biomaterials.2014.02.014","article-title":"Photodynamic antibacterial effect of graphene quantum dots","volume":"35","author":"Ristic","year":"2014","journal-title":"Biomaterials"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"26936","DOI":"10.1021\/acsami.8b08832","article-title":"Degradable Carbon Dots with Broad-Spectrum Antibacterial Activity","volume":"10","author":"Li","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jdent.2018.04.003","article-title":"Influence of zinc oxide quantum dots in the antibacterial activity and cytotoxicity of an experimental adhesive resin","volume":"73","author":"Garcia","year":"2018","journal-title":"J. Dent."},{"key":"ref_32","first-page":"138","article-title":"Role of CdSe quantum dots in the structure and antibacterial activity of chitosan\/poly \u025b-caprolactone thin films","volume":"5","author":"Meikhail","year":"2018","journal-title":"Egypt. J. Basic Appl. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.ejps.2017.07.020","article-title":"Anticancer drug-loaded quantum dots engineered polymeric nanoparticles: Diagnosis\/therapy combined approach","volume":"107","author":"Belletti","year":"2017","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1039\/C0CC04676K","article-title":"Semiconductor quantum dots photosensitizing release of anticancer drug","volume":"47","author":"Liu","year":"2011","journal-title":"Chem. Commun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.coche.2015.11.007","article-title":"Titanium dioxide-based antibacterial surfaces for water treatment","volume":"11","author":"Han","year":"2016","journal-title":"Curr. Opin. Chem. Eng."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Haugen, H., and Lyngstadaas, S. (2016). Antibacterial effects of titanium dioxide in wounds. Wound Healing Biomaterials, Elsevier.","DOI":"10.1016\/B978-1-78242-456-7.00021-0"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4134","DOI":"10.1038\/srep04134","article-title":"Understanding the antimicrobial mechanism of TiO 2-based nanocomposite films in a pathogenic bacterium","volume":"4","author":"Kubacka","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/17435390.2012.742935","article-title":"The antibacterial effects of silver, titanium dioxide and silica dioxide nanoparticles compared to the dental disinfectant chlorhexidine on Streptococcus mutans using a suite of bioassays","volume":"8","author":"Besinis","year":"2014","journal-title":"Nanotoxicology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1023\/B:TMCH.0000014490.54611.5a","article-title":"Synthesis, spectroscopic characterization and antibacterial activity of lanthanide\u2013tetracycline complexes","volume":"29","author":"Karthikeyan","year":"2004","journal-title":"Transit. Met. Chem."},{"key":"ref_40","first-page":"100","article-title":"Synthesis, characterization and antibacterial activity of complex for gadolinium iodide with thiourea","volume":"1","author":"Peng","year":"2011","journal-title":"Appl. Chem. Ind."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3339","DOI":"10.1021\/ja068360v","article-title":"Efficient and Color-Tunable Mn-Doped ZnSe Nanocrystal Emitters:\u2009 Control of Optical Performance via Greener Synthetic Chemistry","volume":"129","author":"Pradhan","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1111\/php.12398","article-title":"Application of CdTe\/ZnSe Quantum Dots in In Vitro Imaging of Chicken Tissue and Embryo","volume":"91","author":"Moulick","year":"2015","journal-title":"Photochem. Photobiol."},{"key":"ref_43","unstructured":"Jelinkova, P., Koudelkova, Z., Milosavljevic, V., Horky, P., Kopel, P., and Adam, V. (2016). Utilization of Selenium Nanoparticles with Schiff Base Chitosan as Antibacterial Agents. MendelNet, 989\u2013993. Available online: https:\/\/pdfs.semanticscholar.org\/6ba5\/800ec37f37ab26508fefcafd3ac3056f3201.pdf."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Herathge, N.D.S., George, J.T., and Rowley, D.A. (2011). Differential antimicrobial activities of Human Beta-Defensins against Methicillin Resistant (MRSA) and Methicillin sensitive (MSSA) Staphylococcus aureus. Science and Technology Against Microbial Pathogens, World Scientific.","DOI":"10.1142\/9789814354868_0003"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Mazumdar, A., Haddad, Y., Milosavljevic, V., Michalkova, H., Guran, R., Bhowmick, S., and Moulick, A. (2020). Peptide-Carbon Quantum Dots conjugate, Derived from Human Retinoic Acid Receptor Responder Protein 2, against Antibiotic-Resistant Gram Positive and Gram Negative Pathogenic Bacteria. Nanomaterials, 10.","DOI":"10.3390\/nano10020325"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.2147\/IDR.S160975","article-title":"Novel vancomycin-peptide conjugate as potent antibacterial agent against vancomycin-resistant Staphylococcus aureus","volume":"11","author":"Jelinkova","year":"2018","journal-title":"Infect. Drug Resist."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/1574-6968.12353","article-title":"Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection","volume":"351","author":"Chudobova","year":"2014","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3531","DOI":"10.1073\/pnas.1217337110","article-title":"Small molecule inhibitor of lipoteichoic acid synthesis is an antibiotic for Gram-positive bacteria","volume":"110","author":"Richter","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s12951-019-0459-1","article-title":"High time resolution and high signal-to-noise monitoring of the bacterial growth kinetics in the presence of plasmonic nanoparticles","volume":"17","author":"Vukomanovic","year":"2019","journal-title":"J. Nanobiotechnol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"38828","DOI":"10.1038\/srep38828","article-title":"General calibration of microbial growth in microplate readers","volume":"6","author":"Stevenson","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"6417","DOI":"10.1074\/jbc.M112.448738","article-title":"Methicillin-resistant Staphylococcus aureus Bacterial Nitric-oxide Synthase Affects Antibiotic Sensitivity and Skin Abscess Development","volume":"288","author":"Beasley","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s10096-017-3089-2","article-title":"In vitro antimicrobial susceptibility testing methods: Agar dilution to 3D tissue-engineered models","volume":"37","author":"Schumacher","year":"2018","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s00217-014-2238-4","article-title":"Effect of glycerol and dihydroxyacetone concentrations in the culture medium on the growth of acetic acid bacteria Gluconobacter oxydans ATCC 621","volume":"239","author":"Gientka","year":"2014","journal-title":"Eur. Food Res. Technol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3517","DOI":"10.1016\/j.molimm.2007.03.013","article-title":"Effects of surfactant protein D on growth, adhesion and epithelial invasion of intestinal Gram-negative bacteria","volume":"44","author":"Hogenkamp","year":"2007","journal-title":"Mol. Immunol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1128\/AAC.37.2.371","article-title":"New turbidimetric assay for quantitation of viable bacterial densities","volume":"37","author":"Li","year":"1993","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_56","first-page":"400","article-title":"Comparison of antimicrobial agents as therapy for experimental endocarditis: Caused by methicillin-resistant Staphylococcus aureus","volume":"37","author":"Sacar","year":"2010","journal-title":"Tex Heart Inst. J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1007\/s12223-010-0060-3","article-title":"Improved protocol for high-quality Co-extraction of DNA and RNA from rumen digesta","volume":"55","author":"Popova","year":"2010","journal-title":"Folia Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Welsh, S., Peakman, T., Sheard, S., and Almond, R. (2017). Comparison of DNA quantification methodology used in the DNA extraction protocol for the UK Biobank cohort. BMC Genom., 18.","DOI":"10.1186\/s12864-016-3391-x"},{"key":"ref_59","unstructured":"(2003). Quantification of DNA and RNA: A Spectrophotometric Method. eLS, Wiley."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1097\/01.gim.0000241904.32039.6f","article-title":"Quantity versus quality: Optimal methods for cell-free DNA isolation from plasma of pregnant women","volume":"8","author":"Jorgez","year":"2006","journal-title":"Genet. Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"474","DOI":"10.2144\/97223st01","article-title":"Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid Purity","volume":"22","author":"Wilfinger","year":"1997","journal-title":"BioTechniques"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Heger, Z., Merlos Rodrigo, M.A., Michalek, P., Polanska, H., Masarik, M., Vit, V., Plevova, M., Pacik, D., Eckschlager, T., and Stiborova, M. (2016). Sarcosine Up-Regulates Expression of Genes Involved in Cell Cycle Progression of Metastatic Models of Prostate Cancer. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0165830"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4172\/2157-7064.1000282","article-title":"Spectroscopic Characterization of Disodium Hydrogen Orthophosphate and Sodium Nitrate after Biofield Treatment","volume":"6","author":"Trivedi","year":"2015","journal-title":"J. Chromatogr. Sep. Tech."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1023\/A:1026081100860","article-title":"FT-IR Spectroscopy of Acid-Modified Titanium Alkoxides: Investigations on the Nature of Carboxylate Coordination and Degree of Complexation","volume":"28","author":"Perrin","year":"2003","journal-title":"J. Sol-Gel Sci. Technol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.jcis.2004.02.012","article-title":"Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria","volume":"275","author":"Sondi","year":"2004","journal-title":"J. Colloid Interface Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"961","DOI":"10.3201\/eid0809.010435","article-title":"First Characterization of a Cluster of VanA-Type Glycopeptide-Resistant Enterococcus faecium Colombia","volume":"8","author":"Diana","year":"2002","journal-title":"Emerg. Infect. Dis. J."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Qureshi, N.K., Yin, S., and Boyle-Vavra, S. (2014). The role of the Staphylococcal VraTSR regulatory system on vancomycin resistance and vanA operon expression in vancomycin-resistant Staphylococcus aureus. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0085873"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/S0022-0728(00)00436-8","article-title":"Scanning electrochemical microscopy of living cells: Part 2. Imaging redox and acid\/basic reactivities","volume":"500","author":"Liu","year":"2001","journal-title":"J. Electroanal. Chem."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Bard, A.J., and Mirkin, M.V. (2012). Scanning Electrochemical Microscopy, CRC Press.","DOI":"10.1201\/b11850"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/S0891-5849(02)00751-7","article-title":"Flavonoids of Inula britannica protect cultured cortical cells from necrotic cell death induced by glutamate","volume":"32","author":"Kim","year":"2002","journal-title":"Free Radic. Biol. Med."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.jep.2004.04.011","article-title":"Reduction of MTT by aqueous herbal extracts in the absence of cells","volume":"93","author":"Shoemaker","year":"2004","journal-title":"J. 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