{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T15:35:36Z","timestamp":1778340936431,"version":"3.51.4"},"reference-count":64,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T00:00:00Z","timestamp":1599696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["LO1508"],"award-info":[{"award-number":["LO1508"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["CZ.2.16\/3.1.00\/21528"],"award-info":[{"award-number":["CZ.2.16\/3.1.00\/21528"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["CEITEC 2020 (LQ1601)"],"award-info":[{"award-number":["CEITEC 2020 (LQ1601)"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CzechNanoLab Research Infrastructure","award":["LM2018110"],"award-info":[{"award-number":["LM2018110"]}]},{"DOI":"10.13039\/501100009553","name":"Agentura Pro Zdravotnick\u00fd V\u00fdzkum \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["17-32285A"],"award-info":[{"award-number":["17-32285A"]}],"id":[{"id":"10.13039\/501100009553","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>Vitiligo is the most common depigmentation disorder of the skin. Currently, its therapy focuses on the halting of the immune response and stimulation of the regenerative processes, leading to the restoration of normal melanocyte function. Platelet-rich plasma (PRP) represents a safe and cheap regenerative therapy option, as it delivers a wide spectrum of native growth factors, cytokines and other bioactive molecules. The aim of this study was to develop a simple delivery system to prolong the effects of the bioactive molecules released from platelets. The surface of electrospun and centrifugally spun poly-\u03b5-caprolactone (PCL) fibrous scaffolds was functionalized with various concentrations of platelets; the influence of the morphology of the scaffolds and the concentration of the released platelet-derived bioactive molecules on melanocytes, was then assessed. An almost two-fold increase in the amount of the released bioactive molecules was detected on the centrifugally spun vs. electrospun scaffolds, and a sustained 14-day release of the bioactive molecules was demonstrated. A strong concentration-dependent response of melanocyte to the bioactive molecules was observed; higher concentrations of bioactive molecules resulted in improved metabolic activity and proliferation of melanocytes. This simple system improves melanocyte viability, offers on-site preparation and is suitable for prolonged topical PRP administration.<\/jats:p>","DOI":"10.3390\/nano10091801","type":"journal-article","created":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T09:10:09Z","timestamp":1599729009000},"page":"1801","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5664-0109","authenticated-orcid":false,"given":"Karolina","family":"Vocetkova","sequence":"first","affiliation":[{"name":"Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic"},{"name":"Department of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic"},{"name":"University Centre for Energy Efficient Buildings, Czech Technical University in Prague, Trinecka 1024, 273 43 Bustehrad, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera","family":"Sovkova","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic"},{"name":"Department of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic"},{"name":"University Centre for Energy Efficient Buildings, Czech Technical University in Prague, Trinecka 1024, 273 43 Bustehrad, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matej","family":"Buzgo","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic"},{"name":"University Centre for Energy Efficient Buildings, Czech Technical University in Prague, Trinecka 1024, 273 43 Bustehrad, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0227-3347","authenticated-orcid":false,"given":"Vera","family":"Lukasova","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic"},{"name":"University Centre for Energy Efficient Buildings, Czech Technical University in Prague, Trinecka 1024, 273 43 Bustehrad, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radek","family":"Divin","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic"},{"name":"Department of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic"},{"name":"University Centre for Energy Efficient Buildings, Czech Technical University in Prague, Trinecka 1024, 273 43 Bustehrad, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michala","family":"Rampichova","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4029-1389","authenticated-orcid":false,"given":"Pavel","family":"Blazek","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology, Brno University of Technology, Purkynova 656\/123, 616 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas","family":"Zikmund","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology, Brno University of Technology, Purkynova 656\/123, 616 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7397-125X","authenticated-orcid":false,"given":"Jozef","family":"Kaiser","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology, Brno University of Technology, Purkynova 656\/123, 616 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zdenek","family":"Karpisek","sequence":"additional","affiliation":[{"name":"Institute of Mathematics, Faculty of Mechanical Engineering, Brno University of Technology, Technicka 2, 616 69 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evzen","family":"Amler","sequence":"additional","affiliation":[{"name":"Department of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic"},{"name":"University Centre for Energy Efficient Buildings, Czech Technical University in Prague, Trinecka 1024, 273 43 Bustehrad, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1028-605X","authenticated-orcid":false,"given":"Eva","family":"Filova","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic"},{"name":"Department of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.jaad.2010.11.061","article-title":"Vitiligo: A comprehensive overview: Part I. Introduction, epidemiology, quality of life, diagnosis, differential diagnosis, associations, histopathology, etiology, and work-up","volume":"65","author":"Alikhan","year":"2011","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Cai, Y., Shi, M., Jiang, S., Cui, S., and Wu, Y. (2016). The Prevalence of Vitiligo: A Meta-Analysis. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0163806"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1111\/j.1346-8138.2010.01139.x","article-title":"Vitiligo: A review of the published work","volume":"38","author":"Yaghoobi","year":"2011","journal-title":"J. Dermatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1111\/ijd.14286","article-title":"Use of platelet-rich plasma to suspend noncultured epidermal cell suspension improves repigmentation after autologous transplantation in stable vitiligo: A double-blind randomized controlled trial","volume":"58","author":"Parambath","year":"2019","journal-title":"Int. J. Dermatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1111\/j.1365-2230.2009.03765.x","article-title":"Vitiligo is more than skin deep: A survey of members of the Vitiligo Society","volume":"35","author":"Talsania","year":"2010","journal-title":"Clin. Exp. Dermatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.jaad.2010.10.043","article-title":"Vitiligo: A comprehensive overview: Part II: Treatment options and approach to treatment","volume":"65","author":"Felsten","year":"2011","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"340","DOI":"10.3109\/09546634.2014.952610","article-title":"Treatment of vitiligo with NB-UVB: A systematic review","volume":"26","author":"Xiao","year":"2015","journal-title":"J. Dermatol. Treat."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.jaad.2008.07.053","article-title":"Narrowband ultraviolet B phototherapy and 308-nm excimer laser in the treatment of vitiligo: A review","volume":"60","author":"Nicolaidou","year":"2009","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/jocd.12194","article-title":"The effect of platelet-rich plasma on the outcome of short-term narrowband\u2013ultraviolet B phototherapy in the treatment of vitiligo: A pilot study","volume":"15","author":"Ibrahim","year":"2016","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1111\/jocd.12397","article-title":"Combined treatment with fractional carbon dioxide laser, autologous platelet-rich plasma, and narrow band ultraviolet B for vitiligo in different body sites: A prospective, randomized comparative trial","volume":"17","author":"Abdelghani","year":"2018","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.tmrv.2014.11.001","article-title":"Platelet Derivatives in Regenerative Medicine: An Update","volume":"29","author":"Sommese","year":"2015","journal-title":"Transfus. Med. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.biomaterials.2015.10.065","article-title":"Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation?","volume":"76","author":"Burnouf","year":"2016","journal-title":"Biomaterials"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.1177\/0363546509349921","article-title":"Platelet-Rich Plasma","volume":"37","author":"Foster","year":"2009","journal-title":"Am. J. Sports Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1097\/DSS.0000000000001083","article-title":"Autologous Pure Platelet-Rich Plasma Dermal Injections for Facial Skin Rejuvenation","volume":"43","author":"Cameli","year":"2017","journal-title":"Dermatol. Surg."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1111\/jocd.12258","article-title":"Assessment of the efficacy and safety of single platelet-rich plasma injection on different types and grades of facial wrinkles","volume":"16","author":"Elnehrawy","year":"2017","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_16","first-page":"191","article-title":"Efficacy of activated 3X platelet-rich plasma in the treatment of androgenic alopecia","volume":"11","author":"James","year":"2016","journal-title":"J. Stem Cells"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1111\/jocd.12207","article-title":"Combined autologous platelet-rich plasma with microneedling verses microneedling with distilled water in the treatment of atrophic acne scars: A concurrent split-face study","volume":"15","author":"Asif","year":"2016","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1111\/jocd.12673","article-title":"Applications and efficacy of platelet-rich plasma in dermatology: A clinical review","volume":"17","author":"Zhang","year":"2018","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actbio.2015.11.007","article-title":"Engineering growth factors for regenerative medicine applications","volume":"30","author":"Mitchell","year":"2016","journal-title":"Acta Biomater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.addr.2017.12.018","article-title":"Blood derivatives awaken in regenerative medicine strategies to modulate wound healing","volume":"129","author":"Mendes","year":"2018","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1038\/nature07039","article-title":"Wound repair and regeneration","volume":"453","author":"Gurtner","year":"2008","journal-title":"Nature"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1089\/ten.2005.11.101","article-title":"Potential of Nanofiber Matrix as Tissue-Engineering Scaffolds","volume":"11","author":"Ma","year":"2005","journal-title":"Tissue Eng."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2011\/290602","article-title":"Polymeric scaffolds in tissue engineering application: A review","volume":"2011","author":"Dhandayuthapani","year":"2011","journal-title":"Int. J. Polym. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1016\/j.polymer.2008.02.002","article-title":"Electrospinning jets and polymer nanofibers","volume":"49","author":"Reneker","year":"2008","journal-title":"Polymer"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.msec.2018.12.069","article-title":"Needleless electrospun and centrifugal spun poly-\u03b5-caprolactone scaffolds as a carrier for platelets in tissue engineering applications: A comparative study with hMSCs","volume":"97","author":"Buzgo","year":"2019","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3263","DOI":"10.2147\/IJN.S63095","article-title":"Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-\u03b5-caprolactone nanofibers and growth factors for prevention of incisional hernia formation","volume":"9","author":"Plencner","year":"2014","journal-title":"Int. J. Nanomed."},{"key":"ref_27","first-page":"530","article-title":"In vivo performance of antibiotic embedded electrospun PCL membranes for prevention of abdominal adhesions","volume":"81","author":"Korkusuz","year":"2007","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_28","first-page":"58","article-title":"Polycaprolactone nanofiber mesh with adhered liposomes as a simple delivery system for bioactive growth factors","volume":"1","author":"Staffa","year":"2017","journal-title":"Transl. Med. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"24","DOI":"10.14311\/APP.2017.8.0024","article-title":"Functionalization of 3D fibrous scaffolds prepared using centrifugal spinning with liposomes as a simple drug delivery system","volume":"8","author":"Rampichova","year":"2017","journal-title":"Acta Polytechnol. CTU Proc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1046\/j.1365-2818.2002.01010.x","article-title":"Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object","volume":"206","author":"Paganin","year":"2002","journal-title":"J. Microsc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1109\/TIM.2019.2910338","article-title":"Characterization of a laboratory-based X-ray computed nanotomography system for propagation-based method of phase contrast imaging","volume":"69","author":"Kalasova","year":"2019","journal-title":"IEEE Trans. Instrum. Meas."},{"key":"ref_32","unstructured":"(2020, May 05). VGStudio MAX, Available online: https:\/\/www.volumegraphics.com."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/jocd.12167","article-title":"Systematic review of the use of platelet-rich plasma in aesthetic dermatology","volume":"14","author":"Leo","year":"2015","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jid.2019.03.1142","article-title":"Harnessing the Power of Regenerative Therapy for Vitiligo and Alopecia Areata","volume":"140","author":"Barbulescu","year":"2020","journal-title":"J. Investig. Dermatol."},{"key":"ref_35","unstructured":"Lim, H., Shin, M., and Lee, M. (2011, January 21\u201324). Clinical application of platelet-rich plasma in vitiligo: A pilot study. Proceedings of the International Pigment Cell Conference, Bordeaux, France."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"551","DOI":"10.2147\/CCID.S178817","article-title":"Platelet-rich plasma versus combined fractional carbon dioxide laser with platelet-rich plasma in the treatment of vitiligo: A comparative study","volume":"11","author":"Kadry","year":"2018","journal-title":"Clin. Cosmet. Investig. Dermatol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1111\/jocd.13103","article-title":"Evaluation of combined excimer laser and platelet-rich plasma for the treatment of nonsegmental vitiligo: A prospective comparative study","volume":"19","author":"Khattab","year":"2019","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_38","first-page":"1","article-title":"Treatment of Vitiligo by PRP and Umbilical Cord Blood: A prospective study in Treatment of Vitiligo by PRP and Umbilical Cord Blood: A prospective study in 120 cases","volume":"18","author":"Saify","year":"2019","journal-title":"IOSR J. Den. Med. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1039\/C6RA26606A","article-title":"Emulsion centrifugal spinning for production of 3D drug releasing nanofibres with core\/shell structure","volume":"7","author":"Buzgo","year":"2017","journal-title":"RSC Adv."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Rogalski, J., Bastiaansen, C., and Peijs, T. (2018). PA6 Nanofibre Production: A Comparison between Rotary Jet Spinning and Electrospinning. Fibers, 6.","DOI":"10.3390\/fib6020037"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4349","DOI":"10.1016\/j.actbio.2012.07.032","article-title":"Influence of the fiber diameter and surface roughness of electrospun vascular grafts on blood activation","volume":"8","author":"Milleret","year":"2012","journal-title":"Acta Biomater."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1177\/0040517515609258","article-title":"Morphology and pore size distribution of electrospun and centrifugal forcespun nylon 6 nanofiber membranes","volume":"86","author":"Krifa","year":"2016","journal-title":"Text. Res. J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"36","DOI":"10.4161\/cam.27477","article-title":"Cell penetration to nanofibrous scaffolds Forcespinning, an alternative approach for fabricating 3D nanofibers","volume":"8","author":"Buzgo","year":"2014","journal-title":"Cell Adhes. Migr."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1111\/j.1365-2184.2011.00737.x","article-title":"Immobilization of thrombocytes on PCL nanofibres enhances chondrocyte proliferation in vitro","volume":"44","author":"Jakubova","year":"2011","journal-title":"Cell Prolif."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1111\/cpr.12276","article-title":"Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells","volume":"49","author":"Vocetkova","year":"2016","journal-title":"Cell Prolif."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1365-2516.2010.02253.x","article-title":"Plasma and cellular contributions to fibrin network formation, structure and stability","volume":"16","author":"Wolberg","year":"2010","journal-title":"Haemophilia"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1002\/ar.20413","article-title":"Ultrastructural comparison of the morphology of three different platelet and fibrin fiber preparations","volume":"290","author":"Pretorius","year":"2007","journal-title":"Anat. Rec."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.addr.2017.12.007","article-title":"Fibrin-based delivery strategies for acute and chronic wound healing","volume":"129","author":"Heher","year":"2018","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1016\/j.biomaterials.2007.12.002","article-title":"The role of actively released fibrin-conjugated VEGF for VEGF receptor 2 gene activation and the enhancement of angiogenesis","volume":"29","author":"Ehrbar","year":"2008","journal-title":"Biomaterials"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.actbio.2016.04.041","article-title":"Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications","volume":"38","author":"Zhang","year":"2016","journal-title":"Acta Biomater."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.jconrel.2010.06.025","article-title":"Fibrin glue as a drug delivery system","volume":"148","author":"Spicer","year":"2010","journal-title":"J. Control. Release"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3772","DOI":"10.1182\/blood.V96.12.3772","article-title":"Vascular endothelial growth factor binds to fibrinogen and fibrin and stimulates endothelial cell proliferation","volume":"96","author":"Sahni","year":"2000","journal-title":"Blood"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"7554","DOI":"10.1074\/jbc.273.13.7554","article-title":"Binding of basic fibroblast growth factor to fibrinogen and fibrin","volume":"273","author":"Sahni","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1046\/j.1538-7836.2003.00250.x","article-title":"FGF-2 but not FGF-1 binds fibrin and supports prolonged endothelial cell growth","volume":"1","author":"Sahni","year":"2003","journal-title":"J. Thromb. Haemost."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1089\/ten.tec.2007.0262","article-title":"Controlled Release of Bioactive Transforming Growth Factor Beta-1 from Fibrin Gels In Vitro","volume":"14","author":"Catelas","year":"2008","journal-title":"Tissue Eng. Part C Methods"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"4563","DOI":"10.1073\/pnas.1221602110","article-title":"Heparin-binding domain of fibrin(ogen) binds growth factors and promotes tissue repair when incorporated within a synthetic matrix","volume":"110","author":"Martino","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1002\/jbm.a.35536","article-title":"Polycaprolactone fiber meshes provide a 3D environment suitable for cultivation and differentiation of melanocytes from the outer root sheath of hair follicle","volume":"104","author":"Savkovic","year":"2016","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1080\/09537104.2017.1316838","article-title":"Platelet lysate as a serum replacement for skin cell culture on biomimetic PCL nanofibers","volume":"29","author":"Sovkova","year":"2018","journal-title":"Platelets"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.jdermsci.2016.05.010","article-title":"Platelet-derived growth factor regulates the proliferation and differentiation of human melanocytes in a differentiation-stage-specific manner","volume":"83","author":"Hirobe","year":"2016","journal-title":"J. Dermatol. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1016\/j.biomaterials.2004.05.002","article-title":"Formation of melanocyte spheroids on the chitosan-coated surface","volume":"26","author":"Lin","year":"2005","journal-title":"Biomaterials"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2011\/532139","article-title":"Amniotic membrane as a scaffold for melanocyte transplantation in patients with stable vitiligo","volume":"532139","author":"Redondo","year":"2011","journal-title":"Dermatol. Res. Pract."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1111\/dsu.12000","article-title":"Efficacy and safety of autologous cultured melanocytes delivered on poly(DL-lactic acid) film: A prospective, open-label, randomized, multicenter study","volume":"38","author":"Ghosh","year":"2012","journal-title":"Dermatol. Surg."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1554","DOI":"10.1038\/sj.jid.5701214","article-title":"Simplifying the delivery of melanocytes and keratinocytes for the treatment of vitiligo using a chemically defined carrier dressing","volume":"128","author":"Eves","year":"2008","journal-title":"J. Investig. Dermatol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1016\/j.biomaterials.2005.08.031","article-title":"Enhanced cell survival of melanocyte spheroids in serum starvation condition","volume":"27","author":"Lin","year":"2006","journal-title":"Biomaterials"}],"container-title":["Nanomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4991\/10\/9\/1801\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:08:34Z","timestamp":1760177314000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4991\/10\/9\/1801"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,10]]},"references-count":64,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["nano10091801"],"URL":"https:\/\/doi.org\/10.3390\/nano10091801","relation":{},"ISSN":["2079-4991"],"issn-type":[{"value":"2079-4991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,10]]}}}