{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T02:06:39Z","timestamp":1790561199078,"version":"4.1.0"},"reference-count":70,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T00:00:00Z","timestamp":1607472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"publisher","award":["POSDRU\/89\/1.5\/S\/60746"],"award-info":[{"award-number":["POSDRU\/89\/1.5\/S\/60746"]}],"id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Romanian Ministry of Education and Research","award":["PCCDI63\/2018 (PN-III-P1-1.2- PCCDI2017-0728);529PED\/2020 (PN-III-P2-2.1-PED-2019-2695) (L.R)"],"award-info":[{"award-number":["PCCDI63\/2018 (PN-III-P1-1.2- PCCDI2017-0728);529PED\/2020 (PN-III-P2-2.1-PED-2019-2695) (L.R)"]}]},{"name":"Romanian National Nucleu Program","award":["LAPLAS VI (contract No. 16N\/2019)"],"award-info":[{"award-number":["LAPLAS VI (contract No. 16N\/2019)"]}]},{"name":"Structural and Functional Proteomics Research Program of the Institute of Biochemistry of the Romanian Academy.","award":["Structural and Functional Proteomics Research Program of the Institute of Biochemistry of the Romanian Academy."],"award-info":[{"award-number":["Structural and Functional Proteomics Research Program of the Institute of Biochemistry of the Romanian Academy."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>Osseous implantology\u2019s material requirements include a lack of potential for inducing allergic disorders and providing both functional and esthetic features for the patient\u2019s benefit. Despite being bioinert, Zirconia ceramics have become a candidate of interest to be used as an alternative to titanium dental and cochlear bone-anchored hearing aid (BAHA) implants, implying the need for endowing the surface with biologically instructive properties by changing basic parameters such as surface texture. Within this context, we propose anisotropic and isotropic patterns (linear microgroove arrays, and superimposed crossline microgroove arrays, respectively) textured in zirconia substrates, as bioinstructive interfaces to guide the cytoskeletal organization of human mesenchymal stem cells (hMSCs). The designed textured micro-nano interfaces with either steep ridges and microgratings or curved edges, and nanoroughened walls obtained by direct femtosecond laser texturing are used to evaluate the hMSC response parameters and osteogenic differentiation to each topography. Our results show parallel micro line anisotropic surfaces are able to guide cell growth only for the steep surfaces, while the curved ones reduce the initial response and show the lowest osteogenic response. An improved osteogenic phenotype of hMSCs is obtained when grown onto isotropic grid\/pillar-like patterns, showing an improved cell coverage and Ca\/P ratio, with direct implications for BAHA prosthetic development, or other future applications in regenerating bone defects.<\/jats:p>","DOI":"10.3390\/nano10122465","type":"journal-article","created":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T09:17:58Z","timestamp":1607505478000},"page":"2465","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3404-4162","authenticated-orcid":false,"given":"Livia","family":"Elena Sima","sequence":"first","affiliation":[{"name":"Department of Molecular Cell Biology, Institute of Biochemistry, Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8508-0916","authenticated-orcid":false,"given":"Anca","family":"Bonciu","sequence":"additional","affiliation":[{"name":"Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, Romania"},{"name":"Faculty of Physics, University of Bucharest, 077125 Magurele, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madalina","family":"Baciu","sequence":"additional","affiliation":[{"name":"Otorhinolaryngology Department, MedLife Clinic, 79-81 Bvd. Tomis, 900178 Constanta, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iulia","family":"Anghel","sequence":"additional","affiliation":[{"name":"Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6180-7073","authenticated-orcid":false,"given":"Luminita Nicoleta","family":"Dumitrescu","sequence":"additional","affiliation":[{"name":"Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5689-8038","authenticated-orcid":false,"given":"Laurentiu","family":"Rusen","sequence":"additional","affiliation":[{"name":"Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3641-5342","authenticated-orcid":false,"given":"Valentina","family":"Dinca","sequence":"additional","affiliation":[{"name":"Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, Romania"},{"name":"IN2-FOTOPLASMAT Center, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/S1369-7021(11)70058-X","article-title":"Biomaterials & scaffolds for tissue engineering","volume":"14","year":"2011","journal-title":"Mater. 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