{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T03:31:20Z","timestamp":1777433480762,"version":"3.51.4"},"reference-count":76,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,3,26]],"date-time":"2021-03-26T00:00:00Z","timestamp":1616716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>Lignin, and its derivatives, are the subject of current research for the exciting properties shown by this biomass. Particularly attractive are lignin nanoparticles for their eco- and biocompatibility compared to other nanomaterials. In this context, the effect of nanostructured lignin microparticles (LNP), obtained from alkaline lignin by acid treatment, on maize plants was investigated. To this end, maize seeds were primed with LNP at five concentrations: 80 mg L\u22121 (T80), 312 mg L\u22121 (T312), 1250 mg L\u22121 (T1250), 5000 mg L\u22121 (T5000) and 20,000 mg L\u22121 (T20000). Concerning the dose applied, LNP prompted positive effects on the first stages of maize development (germination and radicle length). Furthermore, the study of plant growth, biochemical and chemical parameters on the developed plants indicated that concerning the dose applied. LNP stimulated beneficial effects on the seedlings (fresh weight and length of shoots and roots). Besides, specific treatments increased the content of chlorophyll (a and b), carotenoid, and anthocyanin. Finally, the soluble protein content showed a positive trend in response to specific dosages. These effects are significant, given the essential biological function performed by these biomolecules. In conclusion, this research indicates as the nanostructured lignin microparticles can be used, at appropriate dosages, to induce positive biological responses in maize. This beneficial action deserves attention as it candidates LNP for biostimulating a crop through seed priming.<\/jats:p>","DOI":"10.3390\/nano11040846","type":"journal-article","created":{"date-parts":[[2021,3,26]],"date-time":"2021-03-26T06:59:42Z","timestamp":1616741982000},"page":"846","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Lignin Nanoparticles: A Promising Tool to Improve Maize Physiological, Biochemical, and Chemical Traits"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4084-4366","authenticated-orcid":false,"given":"Daniele","family":"Del Buono","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Universit\u00e0 degli Studi di Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8785-5033","authenticated-orcid":false,"given":"Francesca","family":"Luzi","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Perugia, Strada di Pentima 4, 05100 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8515-7813","authenticated-orcid":false,"given":"Debora","family":"Puglia","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Perugia, Strada di Pentima 4, 05100 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3502","DOI":"10.1021\/acssuschemeng.7b03782","article-title":"Valorization of Acid Isolated High Yield Lignin Nanoparticles as Innovative Antioxidant\/Antimicrobial Organic Materials","volume":"6","author":"Yang","year":"2018","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"107685","DOI":"10.1016\/j.biotechadv.2020.107685","article-title":"Lignin Nanoparticles Enter the Scene: A Promising Versatile Green Tool for Multiple Applications","volume":"47","author":"Schneider","year":"2021","journal-title":"Biotechnol. Adv."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3272","DOI":"10.1039\/C7GC00944E","article-title":"From Lignin Subunits to Aggregates: Insights into Lignin Solubilization","volume":"19","author":"Zhao","year":"2017","journal-title":"Green Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.pmatsci.2017.12.001","article-title":"Properties and Chemical Modifications of Lignin: Towards Lignin-Based Nanomaterials for Biomedical Applications","volume":"93","author":"Figueiredo","year":"2018","journal-title":"Prog. Mater. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Do Espirito Santo Pereira, A., Caixeta Oliveira, H., Fernandes Fraceto, L., and Santaella, C. (2021). Nanotechnology Potential in Seed Priming for Sustainable Agriculture. Nanomaterials, 11.","DOI":"10.3390\/nano11020267"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3389\/fnano.2020.579954","article-title":"Nanoparticle-Based Sustainable Agriculture and Food Science: Recent Advances and Future Outlook","volume":"2","author":"Mittal","year":"2020","journal-title":"Front. Nanotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.cej.2019.04.111","article-title":"Ultrafast Adsorption of Heavy Metal Ions onto Functionalized Lignin-Based Hybrid Magnetic Nanoparticles","volume":"372","author":"Zhang","year":"2019","journal-title":"Chem. Eng. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1002\/open.201800157","article-title":"Plant-Based Antioxidant Nanoparticles without Biological Toxicity","volume":"7","author":"Shikinaka","year":"2018","journal-title":"ChemistryOpen"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5492","DOI":"10.1021\/acsanm.9b01083","article-title":"Lignin-Based Magnesium Hydroxide Nanocomposite. Synthesis and Application for the Removal of Potentially Toxic Metals from Aqueous Solution","volume":"2","author":"Ponomarev","year":"2019","journal-title":"ACS Appl. Nano Mater."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7181","DOI":"10.1021\/acssuschemeng.8b01345","article-title":"Application of Lignin and Its Derivatives in Adsorption of Heavy Metal Ions in Water: A Review","volume":"6","author":"Ge","year":"2018","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1515\/zpch-2018-1209","article-title":"Lignin and Lignin Based Materials for the Removal of Heavy Metals from Waste Water-An Overview","volume":"233","author":"Naseer","year":"2019","journal-title":"Z. F\u00fcr Phys. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jconrel.2018.12.012","article-title":"Nano-Based Smart Pesticide Formulations: Emerging Opportunities for Agriculture","volume":"294","author":"Kumar","year":"2019","journal-title":"J. Control. Release"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"6487","DOI":"10.1021\/acs.jafc.7b02178","article-title":"Nanofertilizer for Precision and Sustainable Agriculture: Current State and Future Perspectives","volume":"66","author":"Raliya","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1802315","DOI":"10.1002\/advs.201802315","article-title":"Targeted Drug Delivery in Plants: Enzyme-Responsive Lignin Nanocarriers for the Curative Treatment of the Worldwide Grapevine Trunk Disease Esca","volume":"6","author":"Fischer","year":"2019","journal-title":"Adv. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"9754","DOI":"10.1038\/s41598-017-08571-0","article-title":"Cu-Chitosan Nanoparticle Boost Defense Responses and Plant Growth in Maize (Zea mays L.)","volume":"7","author":"Choudhary","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6148","DOI":"10.1021\/acs.jafc.6b02239","article-title":"Cu-Chitosan Nanoparticle Mediated Sustainable Approach to Enhance Seedling Growth in Maize by Mobilizing Reserved Food","volume":"64","author":"Saharan","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"19768","DOI":"10.1038\/srep19768","article-title":"Nanoparticles Based on Chitosan as Carriers for the Combined Herbicides Imazapic and Imazapyr","volume":"6","author":"Maruyama","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"101079","DOI":"10.1016\/j.bcab.2019.101079","article-title":"Agrochemical Loaded Biocompatible Chitosan Nanoparticles for Insect Pest Management","volume":"18","author":"Sharma","year":"2019","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"10211","DOI":"10.1021\/acssuschemeng.8b01511","article-title":"Preparation of MSNs-Chitosan@Prochloraz Nanoparticles for Reducing Toxicity and Improving Release Properties of Prochloraz","volume":"6","author":"Liang","year":"2018","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.nantod.2014.09.009","article-title":"Agricultural Nanotechnologies: What Are the Current Possibilities?","volume":"10","author":"Parisi","year":"2015","journal-title":"Nano Today"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1039\/C7EN00766C","article-title":"Opportunities to Advance Sustainable Design of Nano-Enabled Agriculture Identified through a Literature Review","volume":"5","author":"Gilbertson","year":"2018","journal-title":"Environ. Sci. Nano"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1002\/cssc.201900480","article-title":"Lignin for Nano- and Microscaled Carrier Systems: Applications, Trends, and Challenges","volume":"12","author":"Sipponen","year":"2019","journal-title":"ChemSusChem"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8241","DOI":"10.1021\/acssuschemeng.7b01903","article-title":"Lignin Nanoparticle as a Novel Green Carrier for the Efficient Delivery of Resveratrol","volume":"5","author":"Dai","year":"2017","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"9342","DOI":"10.1021\/acssuschemeng.8b01652","article-title":"Understanding Lignin Aggregation Processes. A Case Study: Budesonide Entrapment and Stimuli Controlled Release from Lignin Nanoparticles","volume":"6","author":"Sipponen","year":"2018","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_25","first-page":"40","article-title":"Opportunities and Challenges in Biological Lignin Valorization","volume":"42","author":"Beckham","year":"2016","journal-title":"Chem. Biotechnol. Pharm. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"141763","DOI":"10.1016\/j.scitotenv.2020.141763","article-title":"Can Biostimulants Be Used to Mitigate the Effect of Anthropogenic Climate Change on Agriculture? It Is Time to Respond","volume":"751","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"40","DOI":"10.3389\/fpls.2020.00040","article-title":"Editorial: Biostimulants in Agriculture","volume":"11","author":"Rouphael","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1007\/s00344-017-9696-4","article-title":"Humic-Like Water-Soluble Lignins from Giant Reed (Arundo donax L.) Display Hormone-Like Activity on Plant Growth","volume":"36","author":"Savy","year":"2017","journal-title":"J. Plant Growth Regul."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"19958","DOI":"10.3390\/molecules201119671","article-title":"Water-Soluble Lignins from Different Bioenergy Crops Stimulate the Early Development of Maize (Zea mays L.)","volume":"20","author":"Savy","year":"2015","journal-title":"Molecules"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.scitotenv.2018.07.334","article-title":"Bioactivity of Humic Substances and Water Extracts from Compost Made by Ligno-Cellulose Wastes from Biorefinery","volume":"646","author":"Spaccini","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7441","DOI":"10.1021\/acsomega.8b00697","article-title":"One-Pot Transformation of Technical Lignins into Humic-Like Plant Stimulants through Fenton-Based Advanced Oxidation: Accelerating Natural Fungus-Driven Humification","volume":"3","author":"Jeong","year":"2018","journal-title":"ACS Omega"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1016\/j.jclepro.2019.01.018","article-title":"The Potential of Green Synthesized Zinc Oxide Nanoparticles as Nutrient Source for Plant Growth","volume":"214","author":"Singh","year":"2019","journal-title":"J. Clean. Prod."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4235","DOI":"10.1002\/cphc.201200537","article-title":"Fabrication of Environmentally Biodegradable Lignin Nanoparticles","volume":"13","author":"Frangville","year":"2012","journal-title":"ChemPhysChem"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9966","DOI":"10.1021\/acssuschemeng.8b01202","article-title":"Citric Acid as Green Modifier for Tuned Hydrophilicity of Surface Modified Cellulose and Lignin Nanoparticles","volume":"6","author":"He","year":"2018","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.indcrop.2015.05.032","article-title":"Structure and Properties of Biodegradable Wheat Gluten Bionanocomposites Containing Lignin Nanoparticles","volume":"74","author":"Yang","year":"2015","journal-title":"Ind. Crop. Prod."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.plaphy.2016.09.004","article-title":"Enhanced Plant Growth Promoting Role of Phycomolecules Coated Zinc Oxide Nanoparticles with P Supplementation in Cotton (Gossypium hirsutum L.)","volume":"110","author":"Venkatachalam","year":"2017","journal-title":"Plant Physiol. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"F4.3.1","DOI":"10.1002\/0471142913.faf0403s01","article-title":"Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy","volume":"1","author":"Lichtenthaler","year":"2001","journal-title":"Curr. Protoc. Food Anal. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_39","unstructured":"R Core Team (2019). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: http:\/\/www.r-project.org\/."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5263","DOI":"10.1021\/acsabm.0c00637","article-title":"Synergic Effect of Nanolignin and Metal Oxide Nanoparticles into Poly(l-Lactide) Bionanocomposites: Material Properties, Antioxidant Activity, and Antibacterial Performance","volume":"3","author":"Lizundia","year":"2020","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1007\/BF01480830","article-title":"The Colloidal Behaviour of Kraft Lignin III. Swelling Behaviour and Mechanical Properties of Kraft Lignin Gels","volume":"258","author":"Westman","year":"1980","journal-title":"Colloid Polym. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0927-7757(01)00753-1","article-title":"Aggregation of Kraft Lignin Derivatives under Conditions Relevant to the Process, Part I: Phase Behaviour","volume":"194","author":"Norgren","year":"2001","journal-title":"Colloids Surf. Physicochem. Eng. Asp."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1023\/A:1020678430092","article-title":"Effect of the Concentration of Sulfate Lignin on the Aggregation Stability of Its Aqueous Dispersions","volume":"64","author":"Chernoberezhskii","year":"2002","journal-title":"Colloid J."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Ab Rahim, A.H., Man, Z., Sarwono, A., Wan Hamzah, W.S., Yunus, N.M., and Wilfred, C.D. (2018, January 13\u201315). Extraction and Comparative Analysis of Lignin Extract from Alkali and Ionic Liquid Pretreatment. Proceedings of the Institute of Physics Publishing. 5th International Conference on Fundamental and Applied Sciences (ICFAS), Kuala Lumpur, Malaysia.","DOI":"10.1088\/1742-6596\/1123\/1\/012052"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1080\/17458080.2015.1055842","article-title":"Preparation and Characterisation of Lignin Nanoparticles: Evaluation of Their Potential as Antioxidants and UV Protectants","volume":"11","author":"Yearla","year":"2016","journal-title":"J. Exp. Nanosci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.ultsonch.2014.08.021","article-title":"Obtaining Lignin Nanoparticles by Sonication","volume":"23","author":"Gilca","year":"2015","journal-title":"Ultrason. Sonochem."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Bhattacharyya, S., Matsakas, L., Rova, U., and Christakopoulos, P. (2020). Melt Stable Functionalized Organosolv and Kraft Lignin Thermoplastic. Processes, 8.","DOI":"10.3390\/pr8091108"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Cavallo, E., He, X., Luzi, F., Dominici, F., Cerrutti, P., Bernal, C., Foresti, M.L., Torre, L., and Puglia, D. (2020). UV Protective, Antioxidant, Antibacterial and Compostable Polylactic Acid Composites Containing Pristine and Chemically Modified Lignin Nanoparticles. Mol. Basel Switz., 26.","DOI":"10.3390\/molecules26010126"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.biomaterials.2016.12.034","article-title":"In Vitro Evaluation of Biodegradable Lignin-Based Nanoparticles for Drug Delivery and Enhanced Antiproliferation Effect in Cancer Cells","volume":"121","author":"Figueiredo","year":"2017","journal-title":"Biomaterials"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"24915","DOI":"10.1039\/C9RA05064G","article-title":"Continuous Production of Lignin Nanoparticles Using a Microchannel Reactor and Its Application in UV-Shielding Films","volume":"9","author":"Ju","year":"2019","journal-title":"RSC Adv."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Trevisan, H., and Rezende, C.A. (2020). Pure, Stable and Highly Antioxidant Lignin Nanoparticles from Elephant Grass. Ind. Crop. Prod., 145.","DOI":"10.1016\/j.indcrop.2020.112105"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Zhou, Y., Han, Y., Li, G., Yang, S., and Chu, F. (2019). Lignin-Based Hollow Nanoparticles for Controlled Drug Delivery: Grafting Preparation Using \u03b2-Cyclodextrin\/Enzymatic-Hydrolysis Lignin. Nanomaterials, 9.","DOI":"10.3390\/nano9070997"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"581","DOI":"10.3389\/fpls.2020.00581","article-title":"Quantitative Structure-Activity Relationship of Humic-Like Biostimulants Derived From Agro-Industrial Byproducts and Energy Crops","volume":"11","author":"Savy","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Campobenedetto, C., Mannino, G., Agliassa, C., Acquadro, A., Contartese, V., Garabello, C., and Bertea, C.M. (2020). Transcriptome Analyses and Antioxidant Activity Profiling Reveal the Role of a Lignin-Derived Biostimulant Seed Treatment in Enhancing Heat Stress Tolerance in Soybean. Plants, 9.","DOI":"10.3390\/plants9101308"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.indcrop.2007.07.019","article-title":"Lignin and Polyphenols as Allelochemicals","volume":"27","author":"Popa","year":"2008","journal-title":"Ind. Crop. Prod."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"19935","DOI":"10.1021\/acssuschemeng.9b05462","article-title":"When Sustainable Nanochemistry Meets Agriculture: Lignin Nanocapsules for Bioactive Compound Delivery to Plantlets","volume":"7","author":"Falsini","year":"2019","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.plaphy.2020.02.009","article-title":"Versatile Roles of Aquaporin in Physiological Processes and Stress Tolerance in Plants","volume":"149","author":"Singh","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Lima, R.B., Salvador, V.H., Dos Santos, W.D., Bubna, G.A., Finger-Teixeira, A., Soares, A.R., Marchiosi, R., Ferrarese, M.D.L.L., and Ferrarese-Filho, O. (2013). Enhanced Lignin Monomer Production Caused by Cinnamic Acid and Its Hydroxylated Derivatives Inhibits Soybean Root Growth. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0080542"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1007\/s11738-012-1186-5","article-title":"Seed Priming for Abiotic Stress Tolerance: An Overview","volume":"35","author":"Jisha","year":"2013","journal-title":"Acta Physiol. Plant"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s11104-015-2780-2","article-title":"Humic-like Bioactivity on Emergence and Early Growth of Maize (Zea mays L.) of Water-Soluble Lignins Isolated from Biomass for Energy","volume":"402","author":"Savy","year":"2016","journal-title":"Plant Soil"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/BF02902248","article-title":"GA, ABA, Phenol Interaction in the Control of Growth: Phenolic Compounds as Effective Modulators of GA-ABA Interaction in Radish Seedlings","volume":"28","author":"Ray","year":"1986","journal-title":"Biol. Plant"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"836","DOI":"10.3389\/fpls.2020.00836","article-title":"A Biostimulant Seed Treatment Improved Heat Stress Tolerance During Cucumber Seed Germination by Acting on the Antioxidant System and Glyoxylate Cycle","volume":"11","author":"Campobenedetto","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1002\/jsfa.8610","article-title":"Biostimulants from Food Processing By-Products: Agronomic, Quality and Metabolic Impacts on Organic Tomato (Solanum lycopersicum L.): Biostimulants for Enhancing Organic Tomato Quality","volume":"98","author":"Cavoski","year":"2018","journal-title":"J. Sci. Food Agric."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"11940","DOI":"10.1021\/jf202473e","article-title":"Effect of Commercial Lignosulfonate-Humate on Zea mays L. Metabolism","volume":"59","author":"Ertani","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1023\/A:1024214528514","article-title":"Low-Molecular-Weight Organic Acids and Hormone-Like Activity of Dissolved Organic Matter in Two Forest Soils in N Italy","volume":"29","author":"Nardi","year":"2003","journal-title":"J. Chem. Ecol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1023\/A:1006269716762","article-title":"Effect of Phenolic Compounds on the Germination of Six Weeds Species","volume":"28","author":"Reigosa","year":"1999","journal-title":"Plant Growth Regul."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.indcrop.2014.05.039","article-title":"Physiological and Biochemical Responses Induced by Spruce Bark Aqueous Extract and Deuterium Depleted Water with Synergistic Action in Sunflower (Helianthus annuus L.) Plants","volume":"60","author":"Tanase","year":"2014","journal-title":"Ind. Crop. Prod."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"472","DOI":"10.3389\/fpls.2018.00472","article-title":"A Vegetal Biopolymer-Based Biostimulant Promoted Root Growth in Melon While Triggering Brassinosteroids and Stress-Related Compounds","volume":"9","author":"Lucini","year":"2018","journal-title":"Front. Plant Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1007\/s11427-006-2022-1","article-title":"The Anti-Photooxidation of Anthocyanins-Rich Leaves of a Purple Rice Cultivar","volume":"49","author":"Changlian","year":"2006","journal-title":"Sci. China Ser. C"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Sonobe, R., Yamashita, H., Mihara, H., Morita, A., and Ikka, T. (2020). Estimation of Leaf Chlorophyll a, b and Carotenoid Contents and Their Ratios Using Hyperspectral Reflectance. Remote Sens., 12.","DOI":"10.3390\/rs12193265"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/s11099-010-0046-z","article-title":"Coordinate Expression of Light-Harvesting Chlorophyll a\/b Gene Family of Photosystem II and Chlorophyll a Oxygenase Gene Regulated by Salt-Induced Phosphorylation in Dunaliella Salina","volume":"48","author":"Chen","year":"2010","journal-title":"Photosynthetica"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"8757","DOI":"10.1021\/acs.jafc.0c03851","article-title":"Blue:Red LED Light Proportion Affects Vegetative Parameters, Pigment Content, and Oxidative Status of Einkorn ( Triticum monococcum L. ssp","volume":"68","author":"Bartucca","year":"2020","journal-title":"Monococcum ) Wheatgrass. J. Agric. Food Chem."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"109811","DOI":"10.1016\/j.ecoenv.2019.109811","article-title":"Modification of Oxidative Stress through Changes in Some Indicators Related to Phenolic Metabolism in Malva Parviflora Exposed to Cadmium","volume":"187","author":"Zoufan","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Bu, C., Zhang, Q., Zeng, J., Cao, X., Hao, Z., Qiao, D., Cao, Y., and Xu, H. (2020). Identification of a Novel Anthocyanin Synthesis Pathway in the Fungus Aspergillus Sydowii H-1. BMC Genom., 21.","DOI":"10.1186\/s12864-019-6442-2"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"111851","DOI":"10.1016\/j.ecoenv.2020.111851","article-title":"Cadmium Toxicity in Salvia Sclarea L.: An Integrative Response of Element Uptake, Oxidative Stress Markers, Leaf Structure and Photosynthesis","volume":"209","author":"Dobrikova","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"109378","DOI":"10.1016\/j.ecoenv.2019.109378","article-title":"Compared to Antioxidants and Polyamines, the Role of Maize Grain-Derived Organic Biostimulants in Improving Cadmium Tolerance in Wheat Plants","volume":"182","author":"Alzahrani","year":"2019","journal-title":"Ecotoxicol. Environ. 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