{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T23:02:02Z","timestamp":1790550122188,"version":"4.1.0"},"reference-count":32,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T00:00:00Z","timestamp":1621209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>Obtaining a functional tooth is the ultimate goal of tooth engineering. However, the implantation of bioengineered teeth in the jawbone of adult animals never allows for spontaneous eruption due mainly to ankylosis within the bone crypt. The objective of this study was to develop an innovative approach allowing eruption of implanted bioengineered teeth through the isolation of the germ from the bone crypt using a polycaprolactone membrane (PCL). The germs of the first lower molars were harvested on the 14th day of embryonic development, cultured in vitro, and then implanted in the recipient site drilled in the maxillary bone of adult mice. To prevent the ankylosis of the dental germ, a PCL membrane synthesized by electrospinning was placed between the germ and the bone. After 10 weeks of follow-up, microtomography, and histology of the implantation site were performed. In control mice where germs were directly placed in contact with the bone, a spontaneous eruption of bioengineered teeth was only observed in 3.3% of the cases versus 19.2% in the test group where PCL biomembrane was used as a barrier (p &lt; 0.1). This preliminary study is the first to describe an innovative method allowing the eruption of bioengineered tooth implanted directly in the jawbone of mice. This new approach is a hope for the field of tooth regeneration, especially in children with oligodontia in whom titanium implants are not an optimal solution.<\/jats:p>","DOI":"10.3390\/nano11051315","type":"journal-article","created":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T12:19:57Z","timestamp":1621253997000},"page":"1315","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Eruption of Bioengineered Teeth: A New Approach Based on a Polycaprolactone Biomembrane"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0822-225X","authenticated-orcid":false,"given":"C\u00e9line","family":"Stutz","sequence":"first","affiliation":[{"name":"INSERM (French National Institute of Health and Medical Research), UMR 1260, CRBS Regenerative NanoMedicine (RNM), FMTS, 1 rue Eug\u00e8ne Boeckel, 67084 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Clauss","sequence":"additional","affiliation":[{"name":"INSERM (French National Institute of Health and Medical Research), UMR 1260, CRBS Regenerative NanoMedicine (RNM), FMTS, 1 rue Eug\u00e8ne Boeckel, 67084 Strasbourg, France"},{"name":"Faculty of Dentistry, University of Strasbourg (UDS), 8 rue Ste Elisabeth, 67000 Strasbourg, France"},{"name":"Department of Pediatric Dentistry, University Hospitals of Strasbourg (HUS), 1 Place de l\u2019H\u00f4pital, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7988-2290","authenticated-orcid":false,"given":"Olivier","family":"Huck","sequence":"additional","affiliation":[{"name":"INSERM (French National Institute of Health and Medical Research), UMR 1260, CRBS Regenerative NanoMedicine (RNM), FMTS, 1 rue Eug\u00e8ne Boeckel, 67084 Strasbourg, France"},{"name":"Faculty of Dentistry, University of Strasbourg (UDS), 8 rue Ste Elisabeth, 67000 Strasbourg, France"},{"name":"Department of Periodontology, University Hospitals of Strasbourg (HUS), 1 Place de l\u2019H\u00f4pital, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Schulz","sequence":"additional","affiliation":[{"name":"Core Facility Micro- and Nanotomography, Biomaterials Science Center (BMC), Department of Biomedical Engineering, University of Basel, Gewerbestrasse 14, 4123 Allschwil, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3059-3559","authenticated-orcid":false,"given":"Nadia","family":"Benkirane-Jessel","sequence":"additional","affiliation":[{"name":"INSERM (French National Institute of Health and Medical Research), UMR 1260, CRBS Regenerative NanoMedicine (RNM), FMTS, 1 rue Eug\u00e8ne Boeckel, 67084 Strasbourg, France"},{"name":"Faculty of Dentistry, University of Strasbourg (UDS), 8 rue Ste Elisabeth, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabien","family":"Bornert","sequence":"additional","affiliation":[{"name":"INSERM (French National Institute of Health and Medical Research), UMR 1260, CRBS Regenerative NanoMedicine (RNM), FMTS, 1 rue Eug\u00e8ne Boeckel, 67084 Strasbourg, France"},{"name":"Department of Pediatric Dentistry, University Hospitals of Strasbourg (HUS), 1 Place de l\u2019H\u00f4pital, 67000 Strasbourg, France"},{"name":"Department of Oral Medicine and Oral Surgery, University Hospitals of Strasbourg (HUS), 1 Place de l\u2019H\u00f4pital, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7497-1200","authenticated-orcid":false,"given":"Sabine","family":"Kuchler-Bopp","sequence":"additional","affiliation":[{"name":"INSERM (French National Institute of Health and Medical Research), UMR 1260, CRBS Regenerative NanoMedicine (RNM), FMTS, 1 rue Eug\u00e8ne Boeckel, 67084 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5557-674X","authenticated-orcid":false,"given":"Marion","family":"Strub","sequence":"additional","affiliation":[{"name":"INSERM (French National Institute of Health and Medical Research), UMR 1260, CRBS Regenerative NanoMedicine (RNM), FMTS, 1 rue Eug\u00e8ne Boeckel, 67084 Strasbourg, France"},{"name":"Faculty of Dentistry, University of Strasbourg (UDS), 8 rue Ste Elisabeth, 67000 Strasbourg, France"},{"name":"Department of Pediatric Dentistry, University Hospitals of Strasbourg (HUS), 1 Place de l\u2019H\u00f4pital, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/prd.12185","article-title":"Modern implant dentistry based on 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