{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:44:28Z","timestamp":1790405068052,"version":"4.1.0"},"reference-count":117,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T00:00:00Z","timestamp":1697241600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Beijing Municipal Education Commission project","award":["KM202210011002"],"award-info":[{"award-number":["KM202210011002"]}]},{"name":"Beijing Municipal Education Commission project","award":["51972017"],"award-info":[{"award-number":["51972017"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["KM202210011002"],"award-info":[{"award-number":["KM202210011002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["51972017"],"award-info":[{"award-number":["51972017"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>To fight against antibacterial-resistant bacteria-induced infections, the development of highly efficient antibacterial agents with a low risk of inducing resistance is exceedingly urgent. Nanozymes can rapidly kill bacteria with high efficiency by generating reactive oxygen species via enzyme-mimetic catalytic reactions, making them promising alternatives to antibiotics for antibacterial applications. However, insufficient catalytic activity greatly limits the development of nanozymes to eliminate bacterial infection. By increasing atom utilization to the maximum, single-atom nanozymes (SAzymes) with an atomical dispersion of active metal sites manifest superior enzyme-like activities and have achieved great results in antibacterial applications in recent years. In this review, the latest advances in antibacterial SAzymes are summarized, with specific attention to the action mechanism involved in antibacterial applications covering wound disinfection, osteomyelitis treatment, and marine antibiofouling. The remaining challenges and further perspectives of SAzymes for practical antibacterial applications are also discussed.<\/jats:p>","DOI":"10.3390\/nano13202760","type":"journal-article","created":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T14:38:42Z","timestamp":1697294322000},"page":"2760","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Burgeoning Single-Atom Nanozymes for Efficient Bacterial Elimination"],"prefix":"10.3390","volume":"13","author":[{"given":"Tongyu","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Chemistry, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5344-3655","authenticated-orcid":false,"given":"Huanxiang","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2239-347X","authenticated-orcid":false,"given":"Ruilian","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Science, Beijing Jiaotong University, Beijing 100044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1093\/infdis\/jiz134","article-title":"Future Antibacterial Strategies: From Basic Concepts to Clinical Challenges","volume":"220","author":"Cattoir","year":"2019","journal-title":"J. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1038\/nature06536","article-title":"Global Trends in Emerging Infectious Diseases","volume":"451","author":"Jones","year":"2008","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1177\/003335491412900402","article-title":"Antibiotic Resistance: A Public Health Crisis","volume":"129","author":"Lushniak","year":"2014","journal-title":"Public Health Rep."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/nrmicro3155","article-title":"Ribosome-Targeting Antibiotics and Mechanisms of Bacterial Resistance","volume":"12","author":"Wilson","year":"2014","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/s41579-019-0288-0","article-title":"The Global Preclinical Antibacterial Pipeline","volume":"18","author":"Theuretzbacher","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"106103","DOI":"10.1016\/j.ejps.2021.106103","article-title":"Antimicrobial Resistance: Prevalence, Economic Burden, Mechanisms of Resistance and Strategies to Overcome","volume":"170","author":"Pulingam","year":"2022","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"160461","DOI":"10.1016\/j.scitotenv.2022.160461","article-title":"Bacterial Resistance to Antibacterial Agents: Mechanisms, Control Strategies, and Implications for Global Health","volume":"860","author":"Li","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S122","DOI":"10.1038\/nm1145","article-title":"Antibacterial Resistance Worldwide: Causes, Challenges and Responses","volume":"10","author":"Levy","year":"2004","journal-title":"Nat. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1038\/nature.2017.21550","article-title":"The Drug-Resistant Bacteria That Pose the Greatest Health Threats","volume":"543","author":"Willyard","year":"2017","journal-title":"Nature"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1038\/s41579-020-0385-0","article-title":"Biofilm Dispersion","volume":"18","author":"Rumbaugh","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1038\/nrmicro.2017.99","article-title":"Targeting Microbial Biofilms: Current and Prospective Therapeutic Strategies","volume":"15","author":"Koo","year":"2017","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1900608","DOI":"10.1002\/adhm.201900608","article-title":"Rejuvenated Photodynamic Therapy for Bacterial Infections","volume":"8","author":"Jia","year":"2019","journal-title":"Adv. Healthcare Mater."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2103868","DOI":"10.1002\/smll.202103868","article-title":"Chemodynamic Therapy via Fenton and Fenton-Like Nanomaterials: Strategies and Recent Advances","volume":"18","author":"Jia","year":"2022","journal-title":"Small"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"12999","DOI":"10.1039\/D2NR01847K","article-title":"Recent Developments of Sonodynamic Therapy in Antibacterial Application","volume":"14","author":"Wang","year":"2022","journal-title":"Nanoscale"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2003065","DOI":"10.1002\/adma.202003065","article-title":"Metal\u2013Organic-Framework-Engineered Enzyme-Mimetic Catalysts","volume":"32","author":"Ma","year":"2020","journal-title":"Adv. Mater."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Li, H., Zhou, X., Huang, Y., Liao, B., Cheng, L., and Ren, B. (2021). Reactive Oxygen Species in Pathogen Clearance: The Killing Mechanisms, the Adaption Response, and the Side Effects. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.622534"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ren, X., Chen, D., Wang, Y., Li, H., Zhang, Y., Chen, H., Li, X., and Huo, M. (2022). Nanozymes-Recent Development and Biomedical Applications. J. Nanobiotechnol., 20.","DOI":"10.1186\/s12951-022-01295-y"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"257","DOI":"10.15541\/jim20200273","article-title":"Nanozyme: A New Strategy Combating Bacterial","volume":"36","author":"Fu","year":"2020","journal-title":"J. Inorg. Mater."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4357","DOI":"10.1021\/acs.chemrev.8b00672","article-title":"Nanozymes: Classification, Catalytic Mechanisms, Activity Regulation, and Applications","volume":"119","author":"Huang","year":"2019","journal-title":"Chem. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"214170","DOI":"10.1016\/j.ccr.2021.214170","article-title":"Nanozymes: Activity Origin, Catalytic Mechanism, and Biological Application","volume":"448","author":"Yang","year":"2021","journal-title":"Coord. Chem. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1905994","DOI":"10.1002\/adma.201905994","article-title":"Single-Atom Catalysts in Catalytic Biomedicine","volume":"32","author":"Xiang","year":"2020","journal-title":"Adv. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1007\/s12274-022-4856-7","article-title":"Recent Progress in Single-Atom Nanozymes Research","volume":"16","author":"Jiang","year":"2023","journal-title":"Nano Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/s41929-021-00609-x","article-title":"Matching the Kinetics of Natural Enzymes with a Single-Atom Iron Nanozyme","volume":"4","author":"Ji","year":"2021","journal-title":"Nat. Catal."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"12758","DOI":"10.1021\/acs.analchem.1c02842","article-title":"Axial Ligand-Engineered Single-Atom Catalysts with Boosted Enzyme-Like Activity for Sensitive Immunoassay","volume":"93","author":"Xu","year":"2021","journal-title":"Anal. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1007\/s40820-019-0324-7","article-title":"Revealing the Intrinsic Peroxidase-Like Catalytic Mechanism of Heterogeneous Single-Atom Co\u2013MoS2","volume":"11","author":"Wang","year":"2019","journal-title":"Nano-Micro Lett."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"9741","DOI":"10.1039\/D0SC03522J","article-title":"Atomic Engineering of Single-Atom Nanozymes for Enzyme-Like Catalysis","volume":"11","author":"Wu","year":"2020","journal-title":"Chem. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Cai, S., Zhang, W., and Yang, R. (2023). Emerging Single-Atom Nanozymes for Catalytic Biomedical Uses. Nano Res.","DOI":"10.1007\/s12274-023-5864-y"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"108817","DOI":"10.1016\/j.microc.2023.108817","article-title":"Advances in the Application of Single-Atom Nanozymes for Heavy Metal Ion Detection, Tumor Therapy and Antimicrobial Therapy","volume":"191","author":"Wang","year":"2023","journal-title":"Microchem. J."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Hou, J., and Xianyu, Y. (2023). Tailoring the Surface and Composition of Nanozymes for Enhanced Bacterial Binding and Antibacterial Activity. Small, 2302640.","DOI":"10.1002\/smll.202302640"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1992","DOI":"10.1007\/s12274-022-5060-5","article-title":"Single-Atom Nanozymes: From Bench to Bedside","volume":"16","author":"Jin","year":"2023","journal-title":"Nano Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"139411","DOI":"10.1016\/j.cej.2022.139411","article-title":"Nanozymes with Atomically Dispersed Metal Centers: Structure-Activity Relationships and Biomedical Applications","volume":"452","author":"Meng","year":"2023","journal-title":"Chem. Eng. J."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Peng, C., Pang, R., Li, J., and Wang, E. (2023). Current Advances on the Single-Atom Nanozyme and Its Bioapplications. Adv. Mater., 2211724.","DOI":"10.1002\/adma.202211724"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4289","DOI":"10.1039\/D3QI00430A","article-title":"Single-Atom Nanozymes as Promising Catalysts for Biosensing and Biomedical Applications","volume":"10","author":"Xiao","year":"2023","journal-title":"Inorg. Chem. Front."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2300750","DOI":"10.1002\/smll.202300750","article-title":"Engineering Single-Atom Nanozymes for Catalytic Biomedical Applications","volume":"19","author":"Zhu","year":"2023","journal-title":"Small"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"100342","DOI":"10.1016\/j.mtadv.2023.100342","article-title":"Tunable Single-Atom Nanozyme Catalytic System for Biological Applications of Therapy and Diagnosis","volume":"17","author":"Chan","year":"2023","journal-title":"Mater. Today Adv."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1802304","DOI":"10.1002\/adma.201802304","article-title":"Harnessing the Wisdom in Colloidal Chemistry to Make Stable Single-Atom Catalysts","volume":"30","author":"Zhang","year":"2018","journal-title":"Adv. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1801649","DOI":"10.1002\/adma.201801649","article-title":"Fabrication of Single-Atom Catalysts with Precise Structure and High Metal Loading","volume":"30","author":"Wang","year":"2018","journal-title":"Adv. Mater."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Song, H., Zhang, M., and Tong, W. (2022). Single-Atom Nanozymes: Fabrication, Characterization, Surface Modification and Applications of Ros Scavenging and Antibacterial. Molecules, 27.","DOI":"10.3390\/molecules27175426"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1801995","DOI":"10.1002\/adma.201801995","article-title":"Carbon-Supported Single Atom Catalysts for Electrochemical Energy Conversion and Storage","volume":"30","author":"Peng","year":"2018","journal-title":"Adv. Mater."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1900540","DOI":"10.1002\/smtd.201900540","article-title":"Densely Populated Single Atom Catalysts","volume":"4","author":"Wu","year":"2020","journal-title":"Small Methods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2635","DOI":"10.1016\/j.chempr.2021.08.020","article-title":"Single-Atom Engineering of Metal-Organic Frameworks toward Healthcare","volume":"7","author":"Wang","year":"2021","journal-title":"Chem"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"12089","DOI":"10.1021\/acs.chemrev.9b00757","article-title":"Metal-Organic Framework-Based Catalysts with Single Metal Sites","volume":"120","author":"Wei","year":"2020","journal-title":"Chem. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"eaav5490","DOI":"10.1126\/sciadv.aav5490","article-title":"Single-Atom Nanozymes","volume":"5","author":"Huang","year":"2019","journal-title":"Sci. Adv."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2205674","DOI":"10.1002\/adma.202205674","article-title":"Regulating the Coordination Environment of Mesopore-Confined Single Atoms from Metalloprotein-MOFs for Highly Efficient Biocatalysis","volume":"34","author":"Liang","year":"2022","journal-title":"Adv. Mater."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2200331","DOI":"10.1002\/adfm.202200331","article-title":"Reducing Valence States of Co Active Sites in a Single-Atom Nanozyme for Boosted Tumor Therapy","volume":"32","author":"Wang","year":"2022","journal-title":"Adv. Funct. Mater."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"100971","DOI":"10.1016\/j.nantod.2020.100971","article-title":"Boron-Doped Fe-N-C Single-Atom Nanozymes Specifically Boost Peroxidase-Like Activity","volume":"35","author":"Jiao","year":"2020","journal-title":"Nano Today"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1902031","DOI":"10.1002\/adma.201902031","article-title":"Supported Noble-Metal Single Atoms for Heterogeneous Catalysis","volume":"31","author":"Li","year":"2019","journal-title":"Adv. Mater."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1901485","DOI":"10.1002\/smll.201901485","article-title":"Single-Atom Nanozyme Based on Nanoengineered Fe\u2013N\u2013C Catalyst with Superior Peroxidase-Like Activity for Ultrasensitive Bioassays","volume":"15","author":"Cheng","year":"2019","journal-title":"Small"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"11552","DOI":"10.1021\/acsnano.9b05075","article-title":"Nanozyme-Based Bandage with Single-Atom Catalysis for Brain Trauma","volume":"13","author":"Yan","year":"2019","journal-title":"ACS Nano"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1016\/j.joule.2018.06.019","article-title":"Single-Atom Catalysts: Synthetic Strategies and Electrochemical Applications","volume":"2","author":"Chen","year":"2018","journal-title":"Joule"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Li, L., Zhu, Y., Liu, M., Jin, D., Zhang, L., Cheng, J., and Liu, Y. (2021). Conjugation of Oxaliplatin with PEGylated-Nanobody for Enhancing Tumor Targeting and Prolonging Circulation. J. Inorg. Biochem., 223.","DOI":"10.1016\/j.jinorgbio.2021.111553"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"12971","DOI":"10.1002\/anie.202101924","article-title":"Single-Atom Pd Nanozyme for Ferroptosis-Boosted Mild-Temperature Photothermal Therapy","volume":"60","author":"Chang","year":"2021","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.actbio.2023.03.040","article-title":"Modulating the Local Coordination Environment of Cobalt Single-Atomic Nanozymes for Enhanced Catalytic Therapy against Bacteria","volume":"164","author":"Zhang","year":"2023","journal-title":"Acta Biomater."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"112811","DOI":"10.1016\/j.colsurfb.2022.112811","article-title":"Pba Functionalized Single-Atom Fe for Efficient Therapy of Multidrug-Resistant Bacterial Infections","volume":"219","author":"Yang","year":"2022","journal-title":"Colloids Surf. B"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.1038\/s41596-018-0001-1","article-title":"Standardized Assays for Determining the Catalytic Activity and Kinetics of Peroxidase-Like Nanozymes","volume":"13","author":"Jiang","year":"2018","journal-title":"Nat. Protoc."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"5995","DOI":"10.7150\/thno.73681","article-title":"Emerging Nanozyme-Based Multimodal Synergistic Therapies in Combating Bacterial Infections","volume":"12","author":"Zhang","year":"2022","journal-title":"Theranostics"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2206181","DOI":"10.1002\/advs.202206181","article-title":"Engineering Cell Membrane-Cloaked Catalysts as Multifaceted Artificial Peroxisomes for Biomedical Applications","volume":"10","author":"Yang","year":"2023","journal-title":"Adv. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1007\/s12274-020-2678-z","article-title":"Mimicking Peroxidase Active Site Microenvironment by Functionalized Graphene Quantum Dots","volume":"13","author":"Xin","year":"2020","journal-title":"Nano Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2212798","DOI":"10.1002\/adfm.202212798","article-title":"Implanting of Single Zinc Sites into 2D Metal\u2013Organic Framework Nanozymes for Boosted Antibiofilm Therapy","volume":"33","author":"Wang","year":"2023","journal-title":"Adv. Funct. Mater."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Dai, X., Liu, H., Cai, B., Liu, Y., Song, K., Chen, J., Ni, S.-Q., Kong, L., and Zhan, J. (2023). A Bioinspired Atomically Thin Nanodot Supported Single-Atom Nanozyme for Antibacterial Textile Coating. Small, 2303901.","DOI":"10.1002\/smll.202303901"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Ou, H., Qian, Y., Yuan, L., Li, H., Zhang, L., Chen, S., Zhou, M., Yang, G., Wang, D., and Wang, Y. (2023). Spatial Position Regulation of Cu Single Atom Site Realizes Efficient Nanozyme Photocatalytic Bactericidal Activity. Adv. Mater., 2305077.","DOI":"10.1002\/adma.202305077"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e202300119","DOI":"10.1002\/anie.202300119","article-title":"Tuning Local Coordination Environments of Manganese Single-Atom Nanozymes with Multi-Enzyme Properties for Selective Colorimetric Biosensing","volume":"62","author":"Wang","year":"2023","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1021\/acs.nanolett.2c04944","article-title":"Regulating the N Coordination Environment of Co Single-Atom Nanozymes for Highly Efficient Oxidase Mimics","volume":"23","author":"Li","year":"2023","journal-title":"Nano Lett."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/j.chempr.2020.10.023","article-title":"Coordination Number Regulation of Molybdenum Single-Atom Nanozyme Peroxidase-Like Specificity","volume":"7","author":"Wang","year":"2021","journal-title":"Chem"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"18643","DOI":"10.1021\/jacs.1c08581","article-title":"Thermal Atomization of Platinum Nanoparticles into Single Atoms: An Effective Strategy for Engineering High-Performance Nanozymes","volume":"143","author":"Chen","year":"2021","journal-title":"J. Am. Chem. Soc."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"131572","DOI":"10.1016\/j.cej.2021.131572","article-title":"Multiple Application of Sazyme Based on Carbon Nitride Nanorod-Supported Pt Single-Atom for H2O2 Detection, Antibiotic Detection and Antibacterial Therapy","volume":"427","author":"Fan","year":"2022","journal-title":"Chem. Eng. J."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2207507","DOI":"10.1002\/advs.202207507","article-title":"Designing Single-Atom Active Sites on Sp2-Carbon Linked Covalent Organic Frameworks to Induce Bacterial Ferroptosis-Like for Robust Anti-Infection Therapy","volume":"10","author":"Sun","year":"2023","journal-title":"Adv. Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.trac.2018.05.012","article-title":"Nanozyme: An Emerging Alternative to Natural Enzyme for Biosensing and Immunoassay","volume":"105","author":"Wang","year":"2018","journal-title":"Trac Trend Anal. Chem."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"3688","DOI":"10.1039\/D1CS00421B","article-title":"Engineering Single-Atom Catalysts toward Biomedical Applications","volume":"51","author":"Chang","year":"2022","journal-title":"Chem. Soc. Rev."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Shen, L., Khan, M.A., Wu, X., Cai, J., Lu, T., Ning, T., Liu, Z., Lu, W., Ye, D., and Zhao, H. (2022). Fe\u2013N\u2013C Single-Atom Nanozymes Based Sensor Array for Dual Signal Selective Determination of Antioxidants. Biosens. Bioelectron., 205.","DOI":"10.1016\/j.bios.2022.114097"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"7755","DOI":"10.1021\/acsnano.1c11647","article-title":"How to Make Personal Protective Equipment Spontaneously and Continuously Antimicrobial (Incorporating Oxidase-Like Catalysts)","volume":"16","author":"Jin","year":"2022","journal-title":"ACS Nano"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"3364","DOI":"10.1021\/cr050313i","article-title":"Nature\u2019s Inventory of Halogenation Catalysts:\u2009 Oxidative Strategies Predominate","volume":"106","author":"Vaillancourt","year":"2006","journal-title":"Chem. Rev."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1707073","DOI":"10.1002\/adma.201707073","article-title":"Functional Enzyme Mimics for Oxidative Halogenation Reactions That Combat Biofilm Formation","volume":"30","author":"Herget","year":"2018","journal-title":"Adv. Mater."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"2205461","DOI":"10.1002\/adfm.202205461","article-title":"Photothermal-Amplified Single Atom Nanozyme for Biofouling Control in Seawater","volume":"32","author":"Wang","year":"2022","journal-title":"Adv. Funct. Mater."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"4389","DOI":"10.1016\/j.bioactmat.2021.04.024","article-title":"Copper Single-Atom Catalysts with Photothermal Performance and Enhanced Nanozyme Activity for Bacteria-Infected Wound Therapy","volume":"6","author":"Wang","year":"2021","journal-title":"Bioact. Mater."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"0031","DOI":"10.34133\/research.0031","article-title":"Alternative Copper-Based Single-Atom Nanozyme with Superior Multienzyme Activities and Nir-Ii Responsiveness to Fight against Deep Tissue Infections","volume":"6","author":"Bai","year":"2023","journal-title":"Research"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1021\/acsnano.2c11550","article-title":"Single Copper Atom Photocatalyst Powers an Integrated Catalytic Cascade for Drug-Resistant Bacteria Elimination","volume":"17","author":"Wu","year":"2023","journal-title":"ACS Nano"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Fan, X., Gao, Y., Yang, F., Low, J.L., Wang, L., Paulus, B., Wang, Y., Trampuz, A., Cheng, C., and Haag, R. (2023). A Copper Single-Atom Cascade Bionanocatalyst for Treating Multidrug-Resistant Bacterial Diabetic Ulcer. Adv. Funct. Mater., 2301986.","DOI":"10.1002\/adfm.202301986"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"3341","DOI":"10.1016\/j.matt.2022.07.031","article-title":"Emerging Carbon-Supported Single-Atom Catalysts for Biomedical Applications","volume":"5","author":"Liao","year":"2022","journal-title":"Matter"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.jcis.2021.08.054","article-title":"Spherical Mesoporous Fe-N-C Single-Atom Nanozyme for Photothermal and Catalytic Synergistic Antibacterial Therapy","volume":"606","author":"Feng","year":"2022","journal-title":"J. Colloid Interf. Sci."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"135636","DOI":"10.1016\/j.cej.2022.135636","article-title":"Manganese Single-Atom Catalysts for Catalytic-Photothermal Synergistic Anti-Infected Therapy","volume":"438","author":"Xu","year":"2022","journal-title":"Chem. Eng. J."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"139587","DOI":"10.1016\/j.cej.2022.139587","article-title":"Single-Atom-Catalyzed Mxene-Based Nanoplatform with Photo-Enhanced Peroxidase-Like Activity Nanotherapeutics for Staphylococcus Aureus Infection","volume":"452","author":"Li","year":"2023","journal-title":"Chem. Eng. J."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"155866","DOI":"10.1016\/j.apsusc.2022.155866","article-title":"NIR-II-Enhanced Single-Atom-Nanozyme for Sustainable Accelerating Bacteria-Infected Wound Healing","volume":"612","author":"Liu","year":"2023","journal-title":"Appl. Surf. Sci."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.chom.2018.03.018","article-title":"Time Heals All Wounds \u2026 but Wounds Heal Faster with Lactobacillus","volume":"23","author":"Davis","year":"2018","journal-title":"Cell Host Microbe"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1016\/j.drudis.2022.04.020","article-title":"Pharmaceutical Strategies for the Treatment of Bacterial Biofilms in Chronic Wounds","volume":"27","author":"Razdan","year":"2022","journal-title":"Drug Discov. Today"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"5947","DOI":"10.2147\/IJN.S382796","article-title":"Recent Advances in Nanozymes for Bacteria-Infected Wound Therapy","volume":"17","author":"Mo","year":"2022","journal-title":"Int. J. Nanomed."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"4911","DOI":"10.1002\/anie.201813994","article-title":"A Single-Atom Nanozyme for Wound Disinfection Applications","volume":"58","author":"Xu","year":"2019","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"4808","DOI":"10.1007\/s12274-021-3432-x","article-title":"A Highly Accessible Copper Single-Atom Catalyst for Wound Antibacterial Application","volume":"14","author":"Zhao","year":"2021","journal-title":"Nano Res."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1901834","DOI":"10.1002\/smll.201901834","article-title":"Construction of Single-Iron-Atom Nanocatalysts for Highly Efficient Catalytic Antibiotics","volume":"15","author":"Huo","year":"2019","journal-title":"Small"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"2207342","DOI":"10.1002\/advs.202207342","article-title":"Copper-Zinc Bimetallic Single-Atom Catalysts with Localized Surface Plasmon Resonance-Enhanced Photothermal Effect and Catalytic Activity for Melanoma Treatment and Wound-Healing","volume":"10","author":"Liu","year":"2023","journal-title":"Adv. Sci."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/nrmicro.2016.94","article-title":"Biofilms: An Emergent Form of Bacterial Life","volume":"14","author":"Flemming","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"2101374","DOI":"10.1002\/adhm.202101374","article-title":"A Biofilm Microenvironment-Activated Single-Atom Iron Nanozyme with NIR-Controllable Nanocatalytic Activities for Synergetic Bacteria-Infected Wound Therapy","volume":"10","author":"Xu","year":"2021","journal-title":"Adv. Healthcare Mater."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.mattod.2020.12.018","article-title":"Bone Biomaterials for Overcoming Antimicrobial Resistance: Advances in Non-Antibiotic Antimicrobial Approaches for Regeneration of Infected Osseous Tissue","volume":"46","author":"Sadowska","year":"2021","journal-title":"Mater. Today"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"E18","DOI":"10.1093\/cid\/ciq146","article-title":"Clinical Practice Guidelines by the Infectious Diseases Society of America for the Treatment of Methicillin-Resistant Staphylococcus Aureus Infections in Adults and Children","volume":"52","author":"Liu","year":"2011","journal-title":"Clin. Infect. Dis."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"126821","DOI":"10.1016\/j.cej.2020.126821","article-title":"Silicon Incorporation into Hydroxyapatite Nanocarrier Counteracts the Side Effects of Vancomycin for Efficient Chronic Osteomyelitis Treatment","volume":"406","author":"Xu","year":"2021","journal-title":"Chem. Eng. J."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/j.bioactmat.2020.04.020","article-title":"Antibacterial and Cytocompatible Coatings Based on Poly(Adipic Anhydride) for a Ti Alloy Surface","volume":"5","author":"Student","year":"2020","journal-title":"Bioact. Mater."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"2100150","DOI":"10.1002\/adma.202100150","article-title":"Engineering Single-Atomic Iron-Catalyst-Integrated 3d-Printed Bioscaffolds for Osteosarcoma Destruction with Antibacterial and Bone Defect Regeneration Bioactivity","volume":"33","author":"Wang","year":"2021","journal-title":"Adv. Mater."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"2105775","DOI":"10.1002\/smll.202105775","article-title":"Ultrasonic Interfacial Engineering of MoS2-Modified Zn Single-Atom Catalysts for Efficient Osteomyelitis Sonodynamic Ion Therapy","volume":"18","author":"Feng","year":"2022","journal-title":"Small"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"2003587","DOI":"10.1002\/adfm.202003587","article-title":"Sonoactivated Chemodynamic Therapy: A Robust Ros Generation Nanotheranostic Eradicates Multidrug-Resistant Bacterial Infection","volume":"30","author":"Song","year":"2020","journal-title":"Adv. Funct. Mater."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"10628","DOI":"10.1021\/acsnano.1c03424","article-title":"Single-Atom Catalysis for Efficient Sonodynamic Therapy of Methicillin-Resistant Staphylococcus Aureus-Infected Osteomyelitis","volume":"15","author":"Yu","year":"2021","journal-title":"ACS Nano"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/j.progpolymsci.2014.02.002","article-title":"Polymer Brush Coatings for Combating Marine Biofouling","volume":"39","author":"Yang","year":"2014","journal-title":"Prog. Polym. Sci."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s42114-023-00718-0","article-title":"Hybrid Nickel-Molybdenum Bimetallic Sulfide Nanozymes for Antibacterial and Antibiofouling Applications","volume":"6","author":"Wang","year":"2023","journal-title":"Adv. Compos. Hybrid. Mater."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1080\/08927014.2012.700478","article-title":"The Impact and Control of Biofouling in Marine Aquaculture: A Review","volume":"28","author":"Fitridge","year":"2012","journal-title":"Biofouling"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1038\/nature08303","article-title":"Mechanistic Considerations of Halogenating Enzymes","volume":"460","author":"Butler","year":"2009","journal-title":"Nature"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"1603823","DOI":"10.1002\/adma.201603823","article-title":"Haloperoxidase Mimicry by CeO2\u2212x Nanorods Combats Biofouling","volume":"29","author":"Herget","year":"2017","journal-title":"Adv. Mater."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1038\/nnano.2012.91","article-title":"Vanadium Pentoxide Nanoparticles Mimic Vanadium Haloperoxidases and Thwart Biofilm Formation","volume":"7","author":"Natalio","year":"2012","journal-title":"Nat. Nanotechnol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"14218","DOI":"10.1021\/acsami.2c00172","article-title":"Transition Metal Engineering of Molybdenum Disulfide Nanozyme for Biomimicking Anti-Biofouling in Seawater","volume":"14","author":"Luo","year":"2022","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"133483","DOI":"10.1016\/j.cej.2021.133483","article-title":"Single-Atom Tungsten Engineering of Mofs with Biomimetic Antibiofilm Activity toward Robust Uranium Extraction from Seawater","volume":"431","author":"Wang","year":"2022","journal-title":"Chem. Eng. J."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"242","DOI":"10.3103\/S1063455X15050069","article-title":"Generation of Hydrogen Peroxide in the Seawater-Air-Mineral Dynamic System","volume":"37","author":"Kazdobin","year":"2015","journal-title":"J. Water Chem. Technol."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"2105346","DOI":"10.1002\/advs.202105346","article-title":"Atomic Chromium Coordinated Graphitic Carbon Nitride for Bioinspired Antibiofouling in Seawater","volume":"9","author":"Luo","year":"2022","journal-title":"Adv. Sci."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"7482","DOI":"10.1021\/acsnano.1c00894","article-title":"Rough Carbon-Iron Oxide Nanohybrids for near-Infrared-II Light-Responsive Synergistic Antibacterial Therapy","volume":"15","author":"Liu","year":"2021","journal-title":"Acs Nano"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"16236","DOI":"10.1002\/anie.201908289","article-title":"Defect-Rich Adhesive Nanozymes as Efficient Antibiotics for Enhanced Bacterial Inhibition","volume":"58","author":"Cao","year":"2019","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.1021\/acsami.8b12873","article-title":"Surface-Adaptive and Initiator-Loaded Graphene as a Light-Induced Generator with Free Radicals for Drug-Resistant Bacteria Eradication","volume":"11","author":"Yu","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"2000265","DOI":"10.1002\/adhm.202000265","article-title":"Multifunctional Glyco-Nanosheets to Eradicate Drug-Resistant Bacteria on Wounds","volume":"9","author":"Guo","year":"2020","journal-title":"Adv. Healthcare Mater."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.carbon.2019.10.077","article-title":"Boronic Acid Functionalized Graphene Platforms for Diabetic Wound Healing","volume":"158","author":"Beyranvand","year":"2020","journal-title":"Carbon"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/biot.201200313","article-title":"Antimicrobial Enzymes: An Emerging Strategy to Fight Microbes and Microbial Biofilms","volume":"8","author":"Thallinger","year":"2013","journal-title":"Biotechnol. J."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"2328","DOI":"10.1039\/C9NR08467C","article-title":"Enhanced Eradication of Bacterial Biofilms with DNase I-Loaded Silver-Doped Mesoporous Silica Nanoparticles","volume":"12","author":"Tasia","year":"2020","journal-title":"Nanoscale"}],"container-title":["Nanomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4991\/13\/20\/2760\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:06:55Z","timestamp":1760130415000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4991\/13\/20\/2760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,14]]},"references-count":117,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["nano13202760"],"URL":"https:\/\/doi.org\/10.3390\/nano13202760","relation":{},"ISSN":["2079-4991"],"issn-type":[{"value":"2079-4991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,14]]}}}