{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:12:48Z","timestamp":1790809968303,"version":"4.1.0"},"reference-count":25,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T00:00:00Z","timestamp":1701907200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Biomedical Research Networking (CIBER), Spain","award":["RD16\/0006\/0012"],"award-info":[{"award-number":["RD16\/0006\/0012"]}]},{"name":"Biomedical Research Networking (CIBER), Spain","award":["RD16\/0011\/0022"],"award-info":[{"award-number":["RD16\/0011\/0022"]}]},{"name":"Biomedical Research Networking (CIBER), Spain","award":["C17.I1"],"award-info":[{"award-number":["C17.I1"]}]},{"name":"Biomedical Research Networking (CIBER), Spain","award":["PID2020-117977RA-I00"],"award-info":[{"award-number":["PID2020-117977RA-I00"]}]},{"name":"Plan de Recuperaci\u00f3n, Transformaci\u00f3n y Resiliencia","award":["RD16\/0006\/0012"],"award-info":[{"award-number":["RD16\/0006\/0012"]}]},{"name":"Plan de Recuperaci\u00f3n, Transformaci\u00f3n y Resiliencia","award":["RD16\/0011\/0022"],"award-info":[{"award-number":["RD16\/0011\/0022"]}]},{"name":"Plan de Recuperaci\u00f3n, Transformaci\u00f3n y Resiliencia","award":["C17.I1"],"award-info":[{"award-number":["C17.I1"]}]},{"name":"Plan de Recuperaci\u00f3n, Transformaci\u00f3n y Resiliencia","award":["PID2020-117977RA-I00"],"award-info":[{"award-number":["PID2020-117977RA-I00"]}]},{"name":"Plan Complementario de Biotecnolog\u00eda aplicada a la salud","award":["RD16\/0006\/0012"],"award-info":[{"award-number":["RD16\/0006\/0012"]}]},{"name":"Plan Complementario de Biotecnolog\u00eda aplicada a la salud","award":["RD16\/0011\/0022"],"award-info":[{"award-number":["RD16\/0011\/0022"]}]},{"name":"Plan Complementario de Biotecnolog\u00eda aplicada a la salud","award":["C17.I1"],"award-info":[{"award-number":["C17.I1"]}]},{"name":"Plan Complementario de Biotecnolog\u00eda aplicada a la salud","award":["PID2020-117977RA-I00"],"award-info":[{"award-number":["PID2020-117977RA-I00"]}]},{"name":"Spanish Association Against Cancer (AECC)","award":["RD16\/0006\/0012"],"award-info":[{"award-number":["RD16\/0006\/0012"]}]},{"name":"Spanish Association Against Cancer (AECC)","award":["RD16\/0011\/0022"],"award-info":[{"award-number":["RD16\/0011\/0022"]}]},{"name":"Spanish Association Against Cancer (AECC)","award":["C17.I1"],"award-info":[{"award-number":["C17.I1"]}]},{"name":"Spanish Association Against Cancer (AECC)","award":["PID2020-117977RA-I00"],"award-info":[{"award-number":["PID2020-117977RA-I00"]}]},{"name":"Association of Families and Friends of Children with Neuroblastoma (NEN)","award":["RD16\/0006\/0012"],"award-info":[{"award-number":["RD16\/0006\/0012"]}]},{"name":"Association of Families and Friends of Children with Neuroblastoma (NEN)","award":["RD16\/0011\/0022"],"award-info":[{"award-number":["RD16\/0011\/0022"]}]},{"name":"Association of Families and Friends of Children with Neuroblastoma (NEN)","award":["C17.I1"],"award-info":[{"award-number":["C17.I1"]}]},{"name":"Association of Families and Friends of Children with Neuroblastoma (NEN)","award":["PID2020-117977RA-I00"],"award-info":[{"award-number":["PID2020-117977RA-I00"]}]},{"name":"Spanish National R + D + i","award":["RD16\/0006\/0012"],"award-info":[{"award-number":["RD16\/0006\/0012"]}]},{"name":"Spanish National R + D + i","award":["RD16\/0011\/0022"],"award-info":[{"award-number":["RD16\/0011\/0022"]}]},{"name":"Spanish National R + D + i","award":["C17.I1"],"award-info":[{"award-number":["C17.I1"]}]},{"name":"Spanish National R + D + i","award":["PID2020-117977RA-I00"],"award-info":[{"award-number":["PID2020-117977RA-I00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>Using nanoparticles (NPs) in drug delivery has exhibited promising therapeutic potential in various cancer types. Nevertheless, several challenges must be addressed, including the formation of the protein corona, reduced targeting efficiency and specificity, potential immune responses, and issues related to NP penetration and distribution within 3-dimensional tissues. To tackle these challenges, we have successfully integrated iron oxide nanoparticles into neuroblastoma-derived extracellular vesicles (EVs) using the parental labeling method. We first developed a tissue-engineered (TE) neuroblastoma model, confirming the viability and proliferation of neuroblastoma cells for at least 12 days, supporting its utility for EV isolation. Importantly, EVs from long-term cultures exhibited no differences compared to short-term cultures. Concurrently, we designed Rhodamine (Rh) and Polyacrylic acid (PAA)-functionalized magnetite nanoparticles (Fe3O4@PAA-Rh) with high crystallinity, purity, and superparamagnetic properties (average size: 9.2 \u00b1 2.5 nm). We then investigated the internalization of Fe3O4@PAA-Rh nanoparticles within neuroblastoma cells within the TE model. Maximum accumulation was observed overnight while ensuring robust cell viability. However, nanoparticle internalization was low. Taking advantage of the enhanced glucose metabolism exhibited by cancer cells, glucose (Glc)-functionalized nanoparticles (Fe3O4@PAA-Rh-Glc) were synthesized, showing superior cell uptake within the 3D model without inducing toxicity. These glucose-modified nanoparticles were selected for parental labeling of the TE models, showing effective NP encapsulation into EVs. Our research introduces innovative approaches to advance NP delivery, by partially addressing the challenges associated with 3D systems, optimizing internalization, and enhancing NP stability and specificity through EV-based carriers. Also, our findings hold the promise of more precise and effective cancer therapies while minimizing potential side effects.<\/jats:p>","DOI":"10.3390\/nano13243097","type":"journal-article","created":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T05:57:08Z","timestamp":1701928628000},"page":"3097","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Engineering Biomimetic Nanoparticles through Extracellular Vesicle Coating in Cancer Tissue Models"],"prefix":"10.3390","volume":"13","author":[{"given":"Gema","family":"Qui\u00f1onero","sequence":"first","affiliation":[{"name":"Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-3234","authenticated-orcid":false,"given":"Juan","family":"Gallo","sequence":"additional","affiliation":[{"name":"Advanced Magnetic Theranostic Nanostructures Laboratory, International Iberian Nanotechnology Laboratory (INL), 4715-330 Braga, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Carrasco","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1140-3679","authenticated-orcid":false,"given":"Josep","family":"Samitier","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain"},{"name":"Department of Electronic and Biomedical Engineering, University of Barcelona, 08028 Barcelona, Spain"},{"name":"Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7919-9670","authenticated-orcid":false,"given":"Aranzazu","family":"Villasante","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain"},{"name":"Department of Electronic and Biomedical Engineering, University of Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"16078","DOI":"10.1038\/nrdp.2016.78","article-title":"Neuroblastoma","volume":"2","author":"Matthay","year":"2016","journal-title":"Nat. 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