{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T01:38:16Z","timestamp":1780882696599,"version":"3.54.1"},"reference-count":33,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,16]],"date-time":"2023-12-16T00:00:00Z","timestamp":1702684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Vinnova","award":["2018-00739"],"award-info":[{"award-number":["2018-00739"]}]},{"name":"Ad\u00e8me","award":["2018-00739"],"award-info":[{"award-number":["2018-00739"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>The synthesis of core\u2013shell magnetic mesoporous nanoparticles (MMSNs) through a phase transfer process is usually performed at the 100\u2013250 mg scale. At the gram scale, nanoparticles without cores or with multicore systems are observed. Iron oxide core nanoparticles (IO) were synthesized through a thermal decomposition procedure of \u03b1-FeO(OH) in oleic acid. A phase transfer from chloroform to water was then performed in order to wrap the IO nanoparticles with a mesoporous silica shell through the sol\u2013gel procedure. MMSNs were then functionalized with DTPA (diethylenetriaminepentacetic acid) and used for the separation of metal ions. Their toxicity was evaluated. The phase transfer procedure was crucial to obtaining MMSNs on a large scale. Three synthesis parameters were rigorously controlled: temperature, time and glassware. The homogeneous dispersion of MMSNs on the gram scale was successfully obtained. After functionalization with DTPA, the MMSN-DTPAs were shown to have a strong affinity for Ni ions. Furthermore, toxicity was evaluated in cells, zebrafish and seahorse cell metabolic assays, and the nanoparticles were found to be nontoxic. We developed a method of preparing MMSNs at the gram scale. After functionalization with DTPA, the nanoparticles were efficient in metal ion removal and separation; furthermore, no toxicity was noticed up to 125 \u00b5g mL\u22121 in zebrafish.<\/jats:p>","DOI":"10.3390\/nano13243155","type":"journal-article","created":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T05:41:35Z","timestamp":1702878095000},"page":"3155","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Upscale Synthesis of Magnetic Mesoporous Silica Nanoparticles and Application to Metal Ion Separation: Nanosafety Evaluation"],"prefix":"10.3390","volume":"13","author":[{"given":"Mathilde","family":"M\u00e9nard","sequence":"first","affiliation":[{"name":"ICGM, Univ Montpellier, CNRS, ENSCM, 34193 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1176-5335","authenticated-orcid":false,"given":"Lamiaa M. A.","family":"Ali","sequence":"additional","affiliation":[{"name":"IBMM, Univ Montpellier, CNRS, ENSCM, 34193 Montpellier, France"},{"name":"Department of Biochemistry, Medical Research Institute, University of Alexandria, Alexandria 21561, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0928-8525","authenticated-orcid":false,"given":"Ani","family":"Vardanyan","sequence":"additional","affiliation":[{"name":"Department of Molecular Sciences, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8796-3701","authenticated-orcid":false,"given":"Clarence","family":"Charnay","sequence":"additional","affiliation":[{"name":"ICGM, Univ Montpellier, CNRS, ENSCM, 34193 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Raehm","sequence":"additional","affiliation":[{"name":"ICGM, Univ Montpellier, CNRS, ENSCM, 34193 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9rique","family":"Cunin","sequence":"additional","affiliation":[{"name":"ICGM, Univ Montpellier, CNRS, ENSCM, 34193 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0082-546X","authenticated-orcid":false,"given":"Aur\u00e9lie","family":"Bessi\u00e8re","sequence":"additional","affiliation":[{"name":"ICGM, Univ Montpellier, CNRS, ENSCM, 34193 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7828-9137","authenticated-orcid":false,"given":"Erwan","family":"Oliviero","sequence":"additional","affiliation":[{"name":"ICGM, Univ Montpellier, CNRS, ENSCM, 34193 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodossis A.","family":"Theodossiou","sequence":"additional","affiliation":[{"name":"Department of Radiation Biology, Institute for Cancer Research, Radium Hospital, Oslo University Hospital, Montebello, 0379 Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0072-6082","authenticated-orcid":false,"given":"Gulaim A.","family":"Seisenbaeva","sequence":"additional","affiliation":[{"name":"Department of Molecular Sciences, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9641-212X","authenticated-orcid":false,"given":"Magali","family":"Gary-Bobo","sequence":"additional","affiliation":[{"name":"IBMM, Univ Montpellier, CNRS, ENSCM, 34193 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4606-2576","authenticated-orcid":false,"given":"Jean-Olivier","family":"Durand","sequence":"additional","affiliation":[{"name":"ICGM, Univ Montpellier, CNRS, ENSCM, 34193 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"174001","DOI":"10.1088\/1361-6528\/aafe1c","article-title":"Design of hybrid protein-coated magnetic core-mesoporous silica shell nanocomposites for MRI and drug release assessed in a 3D tumor cell model","volume":"30","author":"Meyer","year":"2019","journal-title":"Nanotechnology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4321","DOI":"10.1039\/C7RA12446E","article-title":"A theranostic nanocomposite system based on radial mesoporous silica hybridized with Fe3O4 nanoparticles for targeted magnetic field responsive chemotherapy of breast cancer","volume":"8","author":"Gao","year":"2018","journal-title":"RSC Adv."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5253","DOI":"10.1021\/acs.langmuir.7b04268","article-title":"Magnetic Mesoporous Silica Gated with Doped Carbon Dot for Site-Specific Drug Delivery, Fluorescence, and MR Imaging","volume":"34","author":"Das","year":"2018","journal-title":"Langmuir"},{"key":"ref_4","first-page":"395","article-title":"Inorganic nanomaterials: A brief overview of the applications and developments in sensing and drug delivery","volume":"3","author":"Nasirzadeh","year":"2016","journal-title":"J. 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