{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:36:31Z","timestamp":1791830191101,"version":"4.3.4"},"reference-count":70,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Science and Engineering Research Board (SERB, Govt. of India)","award":["SUR\/2022\/002186"],"award-info":[{"award-number":["SUR\/2022\/002186"]}]},{"name":"the Science and Engineering Research Board (SERB, Govt. of India)","award":["2"],"award-info":[{"award-number":["2"]}]},{"name":"the Science and Engineering Research Board (SERB, Govt. of India)","award":["19"],"award-info":[{"award-number":["19"]}]},{"name":"the Science and Engineering Research Board (SERB, Govt. of India)","award":["2024-3-032"],"award-info":[{"award-number":["2024-3-032"]}]},{"name":"Rashtriya Uchchatar Shiksha Abhiyan (RUSA)","award":["SUR\/2022\/002186"],"award-info":[{"award-number":["SUR\/2022\/002186"]}]},{"name":"Rashtriya Uchchatar Shiksha Abhiyan (RUSA)","award":["2"],"award-info":[{"award-number":["2"]}]},{"name":"Rashtriya Uchchatar Shiksha Abhiyan (RUSA)","award":["19"],"award-info":[{"award-number":["19"]}]},{"name":"Rashtriya Uchchatar Shiksha Abhiyan (RUSA)","award":["2024-3-032"],"award-info":[{"award-number":["2024-3-032"]}]},{"name":"the Science and Technology Bureau of Jinhua City","award":["SUR\/2022\/002186"],"award-info":[{"award-number":["SUR\/2022\/002186"]}]},{"name":"the Science and Technology Bureau of Jinhua City","award":["2"],"award-info":[{"award-number":["2"]}]},{"name":"the Science and Technology Bureau of Jinhua City","award":["19"],"award-info":[{"award-number":["19"]}]},{"name":"the Science and Technology Bureau of Jinhua City","award":["2024-3-032"],"award-info":[{"award-number":["2024-3-032"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>The use of metal nanoparticles is gaining popularity owing to their low cost and high efficacy. We focused on green synthesis of silver nanoparticles (AgNPs) using Tinospora cordifolia (Tc) leaf extracts. The structural characteristics of Tc nanoparticles (TcAgNPs) were determined using several advanced techniques. Pharmacological activities, including antioxidant, anti-inflammatory, and antibacterial properties, were evaluated through in vitro studies. In the results, the change in sample color from yellow to brown after adding silver nitrate revealed the synthesis of TcAgNPs, and the UV\u2013visible spectrum confirmed their formation. X-ray diffraction studies showed the presence of reducing agents and the crystalline nature of the nanoparticles. Fourier-transform infrared spectra revealed the existence of essential secondary metabolites, which act as reducing\/capping agents and stabilize the nanoparticles. The size of the TcAgNPs was small (range 36\u2013168 nm) based on the measurement method. Their negative zeta potential (\u221232.3 mV) ensured their stability in water suspensions. The TcAgNPs were predominantly spherical, as evidenced from scanning electron microscopy and transmission electron microscopy. Atomic absorption spectroscopy data further revealed the conversion of silver nitrate into silver nanoparticles, and thermogravimetric analysis data showed their thermal stability. The TcAgNPs showed significant DPPH\/ABTS radical scavenging ability in a concentration-dependent manner (25\u2013100 \u00b5g\/mL). Membrane lysis assays showed an effective anti-inflammatory activity of the TcAgNPs. Furthermore, the TcAgNPs showed potent antibacterial effects against multidrug-resistant bacteria (Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, and Staphylococcus aureus). The TcAgNPs treatment also exhibited antibiofilm activity against bacterial strains, in a concentration-dependent manner. Our findings demonstrate the structural characteristics of green-synthesized TcAgNPs using advanced techniques. TcAgNPs can be developed as potential antioxidant, anti-inflammatory, and antibacterial drugs.<\/jats:p>","DOI":"10.3390\/nano15050381","type":"journal-article","created":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T09:04:49Z","timestamp":1740992689000},"page":"381","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Green Synthesis and Characterization of Silver Nanoparticles from Tinospora cordifolia Leaf Extract: Evaluation of Their Antioxidant, Anti-Inflammatory, Antibacterial, and Antibiofilm Efficacies"],"prefix":"10.3390","volume":"15","author":[{"given":"Vijaya Durga V. V.","family":"Lekkala","sequence":"first","affiliation":[{"name":"Department of Genetics and Genomics, Yogi Vemana University, Kadapa 516005, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9722-1554","authenticated-orcid":false,"given":"Arun Vasista","family":"Muktinutalapati","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, Yogi Vemana University, Kadapa 516005, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8089-5219","authenticated-orcid":false,"given":"Veeranjaneya Reddy","family":"Lebaka","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yogi Vemana University, Kadapa 516005, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dakshayani","family":"Lomada","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomics, Yogi Vemana University, Kadapa 516005, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4038-1368","authenticated-orcid":false,"given":"Mallikarjuna","family":"Korivi","sequence":"additional","affiliation":[{"name":"College of Physical Education and Health Sciences, Zhejiang Normal University, Jinhua 321004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"College of Physical Education and Health Sciences, Zhejiang Normal University, Jinhua 321004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4885-0437","authenticated-orcid":false,"given":"Madhava C.","family":"Reddy","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, Yogi Vemana University, Kadapa 516005, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"101365","DOI":"10.1016\/j.nanoso.2024.101365","article-title":"Advancements in nanoparticles-based therapies for biomedical applications","volume":"40","author":"Lekkala","year":"2024","journal-title":"Nano-Struct. 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