{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:22:11Z","timestamp":1791346931975,"version":"4.3.1"},"reference-count":53,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Debrecen Scientific Research Bridging Fund (DETKA)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>Selenium (Se) is an essential trace element required for various physiological functions in agriculture. Nanotechnology is applied to produce selenium nanoparticles (SeNPs) that offer new advantages, enhancing their bioavailability and reducing toxicity. To further improve the stability of Se nanoelements in the poultry industry, the grey form of Se was recently offered as a potential alternative. However, its impact on bioaccessibility, metabolism, and overall animal efficiency remains undetermined. This study investigates the impact of red and grey SeNPs on Se content in the liver, blood cellular fraction (BCF), kidney, testis, and eyes, as well as the feed intake (FI) and growth performance, of adult Japanese quails. Adult quails were randomly assigned to five groups: a control (C0) and four groups receiving either red or grey Se nanoparticles (SeNPs) at 0.05 or 0.5 mg\/kg, in addition to the basal diet which already contained 0.042 mg\/kg Se from the premix, resulting in total Se contents of approximately 0.092 and 0.542 mg\/kg in the treatment groups (T1\u2013T4), with four replicates per group. The growth performance of quails fed with nano-Se-supplemented diets showed significant variation across groups (p &lt; 0.05), with body weight differing by up to 20% between the highest performing group (T2) and the lowest (T1). FI showed no significant differences across groups. The results indicated that Se accumulation differed significantly between treatments. The selenium levels in the liver increased in a dose-dependent manner, with the highest accumulation observed in T4 (0.5 mg\/kg grey SeNPs), at 42% above control levels. This pattern suggests that the liver is a primary organ for selenium storage and metabolism. The greatest Se content in BCFs was recorded in the groups that received grey selenium (T3 and T4) and red selenium at high concentrations (T2), while the group given red selenium at low concentrations (T1) and the control (C0) had the lowest Se accumulation. In the kidney tissues and testis, the Se content exhibited no significant differences between the treated groups and the control. The observed variations in the eye and breast muscle Se content among treatment groups reflect the differences in selenium bioavailability, metabolism, and tissue-specific regulatory mechanisms. These findings demonstrate that grey SeNPs can significantly elevate Se bioavailability in quails, particularly in target organs, and enhance the growth performance without notable changes in feed intake. This highlights the potential of SeNPs in enhancing quail nutrition, although further research is needed to establish optimal dosing strategies for safe, effective use.<\/jats:p>","DOI":"10.3390\/nano15110801","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T04:46:38Z","timestamp":1748493998000},"page":"801","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comparative Study of Red and Grey Selenium Nanoparticles on Organ-Specific Selenium Deposition and Growth Performance in Japanese Quails"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2758-0757","authenticated-orcid":false,"given":"Aya","family":"Ferroudj","sequence":"first","affiliation":[{"name":"Nanofood Laboratory, Department of Animal Husbandry, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 138 B\u00f6sz\u00f6rm\u00e9nyi Street, 4032 Debrecen, Hungary"},{"name":"Doctoral School of Animal Husbandry, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5297-2811","authenticated-orcid":false,"given":"Arjun","family":"Muthu","sequence":"additional","affiliation":[{"name":"Nanofood Laboratory, Department of Animal Husbandry, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 138 B\u00f6sz\u00f6rm\u00e9nyi Street, 4032 Debrecen, Hungary"},{"name":"Institute of Agricultural Chemistry and Soil Science, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 138 B\u00f6sz\u00f6rm\u00e9nyi Street, 4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniella","family":"S\u00e1ri","sequence":"additional","affiliation":[{"name":"Nanofood Laboratory, Department of Animal Husbandry, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 138 B\u00f6sz\u00f6rm\u00e9nyi Street, 4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1604-1985","authenticated-orcid":false,"given":"Gr\u00e9ta","family":"T\u00f6r\u0151s","sequence":"additional","affiliation":[{"name":"Nanofood Laboratory, Department of Animal Husbandry, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 138 B\u00f6sz\u00f6rm\u00e9nyi Street, 4032 Debrecen, Hungary"},{"name":"Doctoral School of Animal Husbandry, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5436-6911","authenticated-orcid":false,"given":"\u00c1ron","family":"Beni","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Chemistry and Soil Science, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 138 B\u00f6sz\u00f6rm\u00e9nyi Street, 4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Levente","family":"Czegl\u00e9di","sequence":"additional","affiliation":[{"name":"Doctoral School of Animal Husbandry, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, 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Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 138 B\u00f6sz\u00f6rm\u00e9nyi Street, 4032 Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1007\/s12011-014-0002-z","article-title":"Effects on Liver Hydrogen Peroxide Metabolism Induced by Dietary Selenium Deficiency or Excess in Chickens","volume":"159","author":"Xu","year":"2014","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1080\/1828051X.2020.1786739","article-title":"Effects of Selenium Deficiency on Testis Development and Autophagy in Chicks","volume":"19","author":"Li","year":"2020","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3923\/ijp.2014.160.167","article-title":"Effects of Dietary Nano-Selenium on Tissue Selenium Deposition, Antioxidant Status and Immune Functions in Layer Chicks","volume":"10","author":"Mohapatra","year":"2014","journal-title":"Int. J. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"18557","DOI":"10.1038\/s41598-024-67399-7","article-title":"Selenium Nanoparticle Inclusion in Broiler Diets for Enhancing Sustainable Production and Health","volume":"14","author":"Elkhateeb","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4191","DOI":"10.1007\/s12011-023-03996-3","article-title":"Biological Selenium Nanoparticles in Quail Nutrition: Biosynthesis and Its Impact on Performance, Carcass, Blood Chemistry, and Cecal Microbiota","volume":"202","author":"Reda","year":"2024","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1039\/C9FO02861G","article-title":"The Proteomic Profiling of Multiple Tissue Damage in Chickens for a Selenium Deficiency Biomarker Discovery","volume":"11","author":"Zhang","year":"2020","journal-title":"Food Funct."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1297\/cpe.30.19","article-title":"Thyroid Function in Patients with Selenium Deficiency Exhibits High Free T4 to T3 Ratio","volume":"30","author":"Kobayashi","year":"2021","journal-title":"Clin. Pediatr. Endocrinol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1017\/S0007114523000934","article-title":"Selenium Deficiency-Induced High Concentration of Reactive Oxygen Species Restricts Hypertrophic Growth of Skeletal Muscle in Juvenile Zebrafish by Suppressing TORC1-Mediated Protein Synthesis","volume":"130","author":"Wang","year":"2023","journal-title":"Br. J. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1007\/s12011-011-9266-8","article-title":"Nanoparticles of Selenium as Species with Stronger Physiological Effects in Sheep in Comparison with Sodium Selenite","volume":"146","author":"Sadeghian","year":"2012","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Abdelnour, S.A., Alagawany, M., Hashem, N.M., Farag, M.R., Alghamdi, E.S., Hassan, F.U., Bilal, R.M., Elnesr, S.S., Dawood, M.A.O., and Nagadi, S.A. (2021). Nanominerals: Fabrication Methods, Benefits and Hazards, and Their Applications in Ruminants with Special Reference to Selenium and Zinc Nanoparticles. Animals, 11.","DOI":"10.3390\/ani11071916"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"779305","DOI":"10.3389\/fmars.2021.779305","article-title":"The Effect of Oxidized Fish Oil on the Spleen Index, Antioxidant Activity, Histology and Transcriptome in Juvenile Hybrid Grouper (\u2640 Epinephelus Fuscoguttatus \u00d7 \u2642 Epinephelus lanceolatus)","volume":"8","author":"Long","year":"2021","journal-title":"Front. Mar. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1002\/vms3.650","article-title":"Selenium Nanoparticles Are More Efficient than Sodium Selenite in Reducing the Toxicity of Aflatoxin B1 in Japanese Quail","volume":"8","author":"Khazraei","year":"2022","journal-title":"Vet. Med. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1039\/c3mt00309d","article-title":"Absorption, Distribution, Metabolism and Excretion of Selenium Following Oral Administration of Elemental Selenium Nanoparticles or Selenite in Rats","volume":"6","author":"Loeschner","year":"2014","journal-title":"Metallomics"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Ringuet, M.T., Hunne, B., Lenz, M., Bravo, D.M., and Furness, J.B. (2021). Analysis of Bioavailability and Induction of Glutathione Peroxidase by Dietary Nanoelemental, Organic and Inorganic Selenium. Nutrients, 13.","DOI":"10.3390\/nu13041073"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"942","DOI":"10.2527\/2001.794942x","article-title":"Comparative Effects of High Dietary Levels of Organic and Inorganic Selenium on Selenium Toxicity of Growing-Finishing Pigs","volume":"79","author":"Kim","year":"2001","journal-title":"J. Anim. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"127235","DOI":"10.1016\/j.jtemb.2023.127235","article-title":"Toxicity of Repeated Oral Intake of Organic Selenium, Inorganic Selenium, and Selenium Nanoparticles: A Review","volume":"79","author":"Hadrup","year":"2023","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref_17","first-page":"374","article-title":"Selenosis due to Brazil nut","volume":"40","author":"Ruiz","year":"2024","journal-title":"Med. Int. Mex."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/j.lfs.2004.08.015","article-title":"Comparison of Short-Term Toxicity between Nano-Se and Selenite in Mice","volume":"76","author":"Zhang","year":"2005","journal-title":"Life Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Takahashi, K., Suzuki, N., and Ogra, Y. (2017). Bioavailability Comparison of Nine Bioselenocompounds In Vitro and In Vivo. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18030506"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1093\/jn\/nxaa433","article-title":"Revisiting Selenium Toxicity","volume":"151","author":"Cheng","year":"2021","journal-title":"J. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1002\/ijc.33902","article-title":"Selenium and Cancer Risk: Wide-Angled Mendelian Randomization Analysis","volume":"150","author":"Yuan","year":"2022","journal-title":"Int. J. Cancer"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Alagawany, M., Qattan, S.Y.A., Attia, Y.A., El-Saadony, M.T., Elnesr, S.S., Mahmoud, M.A., Madkour, M., Abd El-Hack, M.E., and Reda, F.M. (2021). Use of Chemical Nano-Selenium as an Antibacterial and Antifungal Agent in Quail Diets and Its Effect on Growth, Carcasses, Antioxidant, Immunity and Caecal Microbes. Animals, 11.","DOI":"10.21203\/rs.3.rs-495839\/v1"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"127122","DOI":"10.1016\/j.ijbiomac.2023.127122","article-title":"Preparation of Ribes nigrum L. Polysaccharides-Stabilized Selenium Nanoparticles for Enhancement of the Anti-Glycation and \u03b1-Glucosidase Inhibitory Activities","volume":"253","author":"Zhao","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1186\/s13568-024-01777-2","article-title":"Nano Selenium in Broiler Feeding: Physiological Roles and Nutritional Effects","volume":"14","author":"Kvan","year":"2024","journal-title":"AMB Express"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"16159","DOI":"10.1007\/s11356-020-07962-7","article-title":"Nanoparticles of Selenium as High Bioavailable and Non-Toxic Supplement Alternatives for Broiler Chickens","volume":"27","author":"Gangadoo","year":"2020","journal-title":"Env. Sci. Pollut. Res. Int."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2107","DOI":"10.2147\/IJN.S157541","article-title":"Nano-Selenium and Its Nanomedicine Applications: A Critical Review","volume":"13","author":"Hosnedlova","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_27","first-page":"289","article-title":"Preparation of Red and Grey Elemental Selenium for Food Fortification","volume":"50","author":"Khandsuren","year":"2021","journal-title":"Acta Aliment."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"133287","DOI":"10.1016\/j.jhazmat.2023.133287","article-title":"Amorphous Structure and Crystal Stability Determine the Bioavailability of Selenium Nanoparticles","volume":"465","author":"Li","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1080\/00071660600830629","article-title":"Effect of Higher Levels of Dietary Selenium on Production Performance and Immune Responses in Growing Japanese Quail","volume":"47","author":"Biswas","year":"2006","journal-title":"Br. Poult. Sci."},{"key":"ref_30","unstructured":"National Research Council Subcommittee on Poultry Nutrition (1994). Nutrient Requirements of Poultry: 1994, National Academies Press."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1007\/s11250-020-02200-1","article-title":"Growth Performance, Hemato-Biochemical Indices, Thyroid Activity, Antioxidant Status, and Immune Response of Growing Japanese Quail Fed Diet with Full-Fat Canola Seeds","volume":"52","author":"Sabic","year":"2020","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1017\/S1466252318000105","article-title":"Effects of Selenium Source and Level in Diet on Glutathione Peroxidase Activity, Tissue Selenium Distribution, and Growth Performance in Poultry","volume":"19","year":"2018","journal-title":"Anim. Health. Res. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"206","DOI":"10.32819\/2020.83030","article-title":"Efficiency of Application of Inorganic and Nanopreparations of Selenium and Probiotics for Growing Young Quails","volume":"8","author":"Tsekhmistrenko","year":"2020","journal-title":"Theor. Appl. Vet. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"skae290","DOI":"10.1093\/jas\/skae290","article-title":"In Ovo Feeding of L-Arginine and Selenium Nanoparticles Influences Post-Hatch Growth, Muscle Development, Antioxidant Status, and Meat Quality in Slow-Growing Chickens","volume":"102","author":"Kaewsatuan","year":"2024","journal-title":"J. Anim. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Ren, Z., Okyere, S.K., Zhang, M., Zhang, X., He, H., and Hu, Y. (2022). Glycine Nano-Selenium Enhances Immunoglobulin and Cytokine Production in Mice Immunized with H9N2 Avian Influenza Virus Vaccine. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23147914"},{"key":"ref_36","unstructured":"European Commission (2022). Commission Implementing Regulation (EU) 2022\/1459 of 2 September 2022 Amending Implementing Regulation (EU) 2019\/804 as Regards the Terms of Authorisation of the Organic Form of Selenium Produced by Saccharomyces Cerevisiae CNCM I-3060 as Feed Additive for All Animal Species (Text with EEA Relevance). Off. J. Eur. Union, 229, 22\u201325."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/s13237-019-00303-1","article-title":"Selenium Nanoparticles Are Less Toxic than Inorganic and Organic Selenium to Mice in Vivo","volume":"62","author":"Bhattacharjee","year":"2019","journal-title":"Nucleus"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"624621","DOI":"10.3389\/fbioe.2020.624621","article-title":"Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles with Different Surface Chemistry and Structure","volume":"8","author":"Zheng","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"680","DOI":"10.3382\/ps.2010-00977","article-title":"Influence of Dietary Nano Elemental Selenium on Growth Performance, Tissue Selenium Distribution, Meat Quality, and Glutathione Peroxidase Activity in Guangxi Yellow Chicken","volume":"90","author":"Zhou","year":"2011","journal-title":"Poult. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1177\/0960327116651124","article-title":"Evaluation of Nanoselenium (Nano-Se) Effect on Hematological and Serum Biochemical Parameters of Rat in Experimentally Lead Poisoning","volume":"36","author":"Dehkordi","year":"2017","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"597","DOI":"10.5455\/javar.2020.g457","article-title":"Dietary Supplementation of Nano-Selenium Improves Reproductive Performance, Sexual Behavior and Deposition of Selenium in the Testis and Ovary of Japanese Quail","volume":"7","author":"Kazaz","year":"2020","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"216","DOI":"10.3382\/ps.0490216","article-title":"Role of Selenium in Neural Physiology of Avian Species: 1. The Distribution of Selenium in Tissues of Chickens, Turkeys and Coturnix","volume":"49","author":"McFarland","year":"1970","journal-title":"Poult. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1001\/jamaophthalmol.2014.3478","article-title":"Age-Related Cataract in Men in the Selenium and Vitamin E Cancer Prevention Trial Eye Endpoints Study: A Randomized Clinical Trial","volume":"133","author":"Christen","year":"2015","journal-title":"JAMA Ophthalmol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"16","DOI":"10.9734\/or\/2023\/v18i6404","article-title":"Vitamin E and Supplements Offer Eye Neuroprotection\u2014Myth or Reality?","volume":"18","author":"Chandrinos","year":"2023","journal-title":"Ophthalmol. Res. Int. J."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Gawor, A., Ruszczynska, A., Czauderna, M., and Bulska, E. (2020). Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods. Animals, 10.","DOI":"10.3390\/ani10050808"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.aquaculture.2015.04.021","article-title":"Effects of Different Levels of Dietary Selenium Nanoparticles on Growth Performance, Muscle Composition, Blood Biochemical Profiles and Antioxidant Status of Common Carp (Cyprinus carpio)","volume":"446","author":"Ashouri","year":"2015","journal-title":"Aquaculture"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"265","DOI":"10.17221\/361-CJAS","article-title":"Effect of Dietary Selenium Sources on Growth Performance, Breast Muscle Selenium, Glutathione Peroxidase Activity and Oxidative Stability in Broilers","volume":"53","author":"Zita","year":"2008","journal-title":"Czech J. Anim. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.aquaculture.2009.03.007","article-title":"Effects of Different Dietary Selenium Sources (Selenium Nanoparticle and Selenomethionine) on Growth Performance, Muscle Composition and Glutathione Peroxidase Enzyme Activity of Crucian Carp (Carassius auratus gibelio)","volume":"291","author":"Zhou","year":"2009","journal-title":"Aquaculture"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1071\/AN19581","article-title":"Nano Selenium Improves Humoral Immunity, Growth Performance and Breast-Muscle Selenium Concentration of Broiler Chickens","volume":"60","author":"Mohammadi","year":"2020","journal-title":"Anim. Prod. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Mahmoud, R., Salama, B., Safhi, F.A., Pet, I., Pet, E., and Ateya, A. (2024). Assessing the Impacts of Different Levels of Nano-Selenium on Growth Performance, Serum Metabolites, and Gene Expression in Heat-Stressed Growing Quails. Vet. Sci., 11.","DOI":"10.3390\/vetsci11060228"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"31","DOI":"10.2754\/avb201281010031","article-title":"Effects of Increased Content of Organic Selenium in Feed on the Selenium Content and Fatty Acid Profile in Broiler Breast Muscle","volume":"81","author":"Kralik","year":"2012","journal-title":"Acta Vet. Brno"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/s12011-020-02052-8","article-title":"Selenomethionine Improves Antioxidant Capacity of Breast Muscle in Geese Via Stimulating Glutathione System and Thiol Pool","volume":"198","author":"Wan","year":"2020","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1017\/S1751731113002395","article-title":"Meta-Analysis of Selenium Accumulation and Expression of Antioxidant Enzymes in Chicken Tissues","volume":"8","author":"Zoidis","year":"2014","journal-title":"Animal"}],"container-title":["Nanomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4991\/15\/11\/801\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:41:22Z","timestamp":1760031682000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4991\/15\/11\/801"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,27]]},"references-count":53,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["nano15110801"],"URL":"https:\/\/doi.org\/10.3390\/nano15110801","relation":{},"ISSN":["2079-4991"],"issn-type":[{"value":"2079-4991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,27]]}}}