{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T19:17:13Z","timestamp":1789327033827,"version":"build-2803163510"},"reference-count":54,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2014NextGenerationEU, Mission 4 Component 2, Inv. 1.5","award":["CUP B83C22003920001"],"award-info":[{"award-number":["CUP B83C22003920001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>We present a dual biosensing strategy integrating Quartz Crystal Microbalance (QCM) and Surface-Enhanced Raman Spectroscopy (SERS) for the quantitative and molecular-specific detection of FKBP12. Silver nanodendritic arrays were electrodeposited onto QCM sensors, optimized for SERS enhancement using Rhodamine 6G, and functionalized with a custom-designed receptor to selectively capture FKBP12. QCM measurements revealed a two-step Langmuir adsorption behavior, enabling sensitive mass quantification with a low limit of detection. Concurrently, in situ SERS analysis on the same sensor provided vibrational fingerprints of FKBP12, resolved through comparative studies of the free protein, surface-bound receptor, and surface-bound receptor\u2013protein complex. Ethanol-induced denaturation confirmed protein-specific peaks, while shifts in receptor vibrational modes\u2014linked to FKBP12 binding\u2014demonstrated dynamic molecular interactions. A ratiometric parameter, derived from key peak intensities, served as a robust, concentration-dependent signature of complex formation. This platform bridges quantitative (QCM) and structural (SERS) biosensing, offering real-time mass tracking and conformational insights. The nanodendritic substrate\u2019s dual functionality, combined with the receptor\u2019s selectivity, advances label-free protein detection for applications in drug diagnostics, with potential adaptability to other target analytes.<\/jats:p>","DOI":"10.3390\/nano15161230","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T15:51:02Z","timestamp":1755013862000},"page":"1230","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Combining QCM and SERS on a Nanophotonic Chip: A Dual-Functional Sensor for Biomolecular Interaction Analysis and Protein Fingerprinting"],"prefix":"10.3390","volume":"15","author":[{"given":"Cosimo","family":"Bartolini","sequence":"first","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy"},{"name":"Center for Colloid and Surface Science (CSGI), University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Tozzetti","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy"},{"name":"Center for Colloid and Surface Science (CSGI), University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3112-6680","authenticated-orcid":false,"given":"Cristina","family":"Gellini","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7894-7737","authenticated-orcid":false,"given":"Marilena","family":"Ricci","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6745-7484","authenticated-orcid":false,"given":"Stefano","family":"Menichetti","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2667-3847","authenticated-orcid":false,"given":"Piero","family":"Procacci","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5947-7757","authenticated-orcid":false,"given":"Gabriella","family":"Caminati","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cUgo Schiff\u201d, University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy"},{"name":"Center for Colloid and Surface Science (CSGI), University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, FI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.2144\/000113382","article-title":"Antibody Validation","volume":"48","author":"Bordeaux","year":"2010","journal-title":"Biotechniques"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.4103\/1673-5374.284980","article-title":"Mounting Evidence of FKBP12 Implication in Neurodegeneration","volume":"15","author":"Caminati","year":"2020","journal-title":"Neural Regen. 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