{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:54:06Z","timestamp":1791852846039,"version":"4.3.4"},"reference-count":44,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LQ24C200009"],"award-info":[{"award-number":["LQ24C200009"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32472484"],"award-info":[{"award-number":["32472484"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["U24A20475"],"award-info":[{"award-number":["U24A20475"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32502405"],"award-info":[{"award-number":["32502405"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2023YFD2100802"],"award-info":[{"award-number":["2023YFD2100802"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>In this study, the structural changes and reconstruction mechanism of sesame protein-derived amyloid fibrils under varied digestive parameters (pepsin concentration, digestive pH and ionic strength) during gastric digestion were investigated, and the effect of fibril reconstruction on the gastric digestion stability of \u03b2-carotene nanoparticles was also explored. The results demonstrated that amyloid fibrils underwent a three-stage dynamic process of enzymatic hydrolysis, regeneration and degradation during gastric digestion. The pepsin concentration of 2 mg\/mL was found to promote the balance between fibril hydrolysis and regeneration. The fibrils displayed a pronounced regenerative capacity at pH values of 1.5 and 2.5, whereas at pH 3.5, which was proximal to the isoelectric point of protein, aggregation and precipitation were observed. Furthermore, it was found that 10 mM NaCl exerted minimal influence on fibril stability, whereas the higher concentrations of salt ions were shown to obstruct regeneration and promote aggregation. Analyses through SDS-PAGE, GPC, and MALDI-TOF-MS revealed a gradual reduction in the molecular weight of the fibrils during gastric digestion, with certain fragments reaggregating to form new fibril structures. The fibril-based delivery system formed a stable protective structure for \u03b2-carotene nanoparticles, which not only prevented their aggregation but also facilitated their release in the small intestine.<\/jats:p>","DOI":"10.3390\/nano15231829","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T10:34:01Z","timestamp":1764758041000},"page":"1829","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Reconstruction of Sesame Protein-Derived Amyloid Fibrils Alleviates the Gastric Digestion Instability of \u03b2-Carotene Nanoparticles"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6643-3069","authenticated-orcid":false,"given":"Liang","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing 314100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puxuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Technology Research Institute, CR Snow Breweries, Beijing 100010, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haocheng","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing 314100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing 314100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6375-5722","authenticated-orcid":false,"given":"Tengfei","family":"Yu","sequence":"additional","affiliation":[{"name":"Technology Research Institute, CR Snow Breweries, Beijing 100010, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8773-8504","authenticated-orcid":false,"given":"Guanchen","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing 314100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghong","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"},{"name":"Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing 314100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"142559","DOI":"10.1016\/j.foodchem.2024.142559","article-title":"Plant-based protein amyloid fibrils: Origins, formation, extraction, applications, and safety","volume":"469","author":"Liang","year":"2025","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Yu, Z., Li, N., Liu, Y., Zhang, B., Zhang, M., Wang, X., and Wang, X. (2024). Formation, structure and functional characteristics of amyloid fibrils formed based on soy protein isolates. Int. J. Biol. Macromol., 254.","DOI":"10.1016\/j.ijbiomac.2023.127956"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6806","DOI":"10.1038\/s41467-023-42486-x","article-title":"Food amyloid fibrils are safe nutrition ingredients based on in-vitro and in-vivo assessment","volume":"14","author":"Xu","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2760","DOI":"10.1021\/acsnano.9b09125","article-title":"Amyloid-Polyphenol Hybrid Nanofilaments Mitigate Colitis and Regulate Gut Microbial Dysbiosis","volume":"14","author":"Hu","year":"2020","journal-title":"ACS Nano"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3385","DOI":"10.1021\/acsnano.7b08969","article-title":"Polyphenol-Binding Amyloid Fibrils Self-Assemble into Reversible Hydrogels with Antibacterial Activity","volume":"12","author":"Hu","year":"2018","journal-title":"ACS Nano"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"110995","DOI":"10.1016\/j.foodhyd.2024.110995","article-title":"Astaxanthin complexes of five plant protein fibrils: Aggregation behavior, interaction, and delivery properties","volume":"162","author":"Wang","year":"2025","journal-title":"Food Hydrocoll."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"141849","DOI":"10.1016\/j.foodchem.2024.141849","article-title":"Amyloid fibrils for \u03b2-carotene delivery\u2014Influence of self-assembled structures on binding and in vitro release behavior","volume":"464","author":"Chen","year":"2025","journal-title":"Food Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e2401172","DOI":"10.1002\/advs.202401172","article-title":"Overcoming Biopotency Barriers: Advanced Oral Delivery Strategies for Enhancing the Efficacy of Bioactive Food Ingredients","volume":"11","author":"Liu","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8106","DOI":"10.1039\/C9FO00961B","article-title":"Formation of fibrils derived from whey protein isolate: Structural characteristics and protease resistance","volume":"10","author":"Hu","year":"2019","journal-title":"Food Funct."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.ijbiomac.2022.12.104","article-title":"Proteolysis-driven proliferation and rigidification of pepsin-resistant amyloid fibrils","volume":"227","author":"Cheong","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.foodchem.2015.07.044","article-title":"Evaluation of protease resistance and toxicity of amyloid-like food fibrils from whey, soy, kidney bean, and egg white","volume":"192","author":"Ulluwishewa","year":"2016","journal-title":"Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"193","DOI":"10.48130\/FIA-2023-0021","article-title":"A review on protein based nanocarriers for polyphenols: Interaction and stabilization mechanisms","volume":"2","author":"Xiao","year":"2023","journal-title":"Food Innov. Adv."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"111019","DOI":"10.1016\/j.foodhyd.2024.111019","article-title":"Functionalization of oilseed proteins via fibrillization: Comparison in structural characteristics, digestibility and 3D printability of amyloid fibrils","volume":"162","author":"Zhang","year":"2025","journal-title":"Food Hydrocoll."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"244","DOI":"10.48130\/fia-0024-0022","article-title":"Physical, thermal, and storage stability of multilayered emulsion loaded with \u03b2-carotene","volume":"3","author":"Sivabalan","year":"2024","journal-title":"Food Innov. Adv."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"222","DOI":"10.48130\/fia-0024-0021","article-title":"Protection mechanism of \u03b2-carotene on the chlorophyll photostability through aggregation: A quantum chemical perspective","volume":"3","author":"Li","year":"2024","journal-title":"Food Innov. 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Eng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"108043","DOI":"10.1016\/j.foodhyd.2022.108043","article-title":"Impact of biopolymer-surfactant interactions on the particle aggregation inhibition of \u03b2-carotene in high loaded microcapsules: Spontaneous dispersibility and in vitro digestion","volume":"134","author":"Zhang","year":"2023","journal-title":"Food Hydrocoll."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"109155","DOI":"10.1016\/j.foodcont.2022.109155","article-title":"Thermal-induced impact on physicochemical property and bioaccessibility of \u03b2-carotene in aqueous suspensions fabricated by wet-milling approach","volume":"141","author":"Zhang","year":"2022","journal-title":"Food Control"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"102750","DOI":"10.1016\/j.cis.2022.102750","article-title":"Recent progresses in the delivery of \u03b2-carotene: From nano\/microencapsulation to bioaccessibility","volume":"307","author":"Rostamabadi","year":"2022","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.foodhyd.2017.09.034","article-title":"Factors affecting the bioaccessibility of \u03b2-carotene in lipid-based microcapsules: Digestive conditions, the composition, structure and physical state of microcapsules","volume":"77","author":"Lin","year":"2018","journal-title":"Food Hydrocoll."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.foodhyd.2018.02.001","article-title":"Application of ultrasound pretreatment and glycation in regulating the heat-induced amyloid-like aggregation of \u03b2-lactoglobulin","volume":"80","author":"Zhao","year":"2018","journal-title":"Food Hydrocoll."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"313","DOI":"10.48130\/FIA-2023-0031","article-title":"Development of \u03ba-carrageenan hydrogels with mechanically stronger structures via a solvent-replacement method","volume":"2","author":"Wang","year":"2023","journal-title":"Food Innov. Adv."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wang, X., Hu, Y., Cao, Z., Liang, X., Zhang, Y., Jiang, L., Xu, Z., and Sui, X. (2024). Effect of protease hydrolysis on the structure of acidic heating-induced soy protein amyloid fibrils. Int. J. Biol. Macromol., 282.","DOI":"10.1016\/j.ijbiomac.2024.137100"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"111367","DOI":"10.1016\/j.foodhyd.2025.111367","article-title":"Interfacial and oil-in-water emulsifying properties of ovalbumin enriched in amyloid-like fibrils and peptides","volume":"166","author":"Huyst","year":"2025","journal-title":"Food Hydrocoll."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"111300","DOI":"10.1016\/j.foodhyd.2025.111300","article-title":"Formation and morphology of flaxseed protein isolate amyloid fibrils as governed by NaCl concentration","volume":"166","author":"Wang","year":"2025","journal-title":"Food Hydrocoll."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"111691","DOI":"10.1016\/j.jfoodeng.2023.111691","article-title":"Modulating physicochemical properties of \u03b2-carotene in the microcapsules by polyphenols co-milling","volume":"359","author":"Zhang","year":"2023","journal-title":"J. Food Eng."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.foodhyd.2017.06.024","article-title":"Effects of calcium on lipid digestion in nanoemulsions stabilized by modified starch: Implications for bioaccessibility of \u03b2-carotene","volume":"73","author":"Lin","year":"2017","journal-title":"Food Hydrocoll."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7120","DOI":"10.1021\/acs.jafc.9b01063","article-title":"Effects of Starch on the Digestibility of Gluten under Different Thermal Processing Conditions","volume":"67","author":"Wen","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"111549","DOI":"10.1016\/j.foodhyd.2025.111549","article-title":"Effects of high hydrostatic pressure treatment on structural, techno-functional and rheological properties of sesame protein isolate","volume":"168","author":"Parlak","year":"2025","journal-title":"Food Hydrocoll."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"109499","DOI":"10.1016\/j.foodhyd.2023.109499","article-title":"Influence of amyloid fibril length and ionic strength on WPI-based fiber-hydrogel composites: Microstructural, rheological and water holding properties","volume":"148","author":"Khalesi","year":"2024","journal-title":"Food Hydrocoll."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.idairyj.2010.02.014","article-title":"Tuning the properties of \u03b2-lactoglobulin nanofibrils with pH, NaCl and CaCl2","volume":"20","author":"Loveday","year":"2010","journal-title":"Int. Dairy J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"110659","DOI":"10.1016\/j.foodhyd.2024.110659","article-title":"Bromelain hydrolysis and CaCl2 coordination promote the fibrillation of quinoa protein at pH 7","volume":"159","author":"Fu","year":"2025","journal-title":"Food Hydrocoll."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"109604","DOI":"10.1016\/j.foodhyd.2023.109604","article-title":"Production and characterization of amaranth amyloid fibrils from food protein waste","volume":"149","author":"Mykolenko","year":"2024","journal-title":"Food Hydrocoll."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"108444","DOI":"10.1016\/j.foodhyd.2022.108444","article-title":"Improvement of the encapsulation capacity and emulsifying properties of soy protein isolate through controlled enzymatic hydrolysis","volume":"138","author":"Lv","year":"2023","journal-title":"Food Hydrocoll."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"115654","DOI":"10.1016\/j.foodres.2024.115654","article-title":"Enhancing the physical and oxidative stability of hempseed protein emulsion via comparative enzymolysis with different proteases: Interfacial properties of the adsorption layer","volume":"201","author":"Wang","year":"2025","journal-title":"Food Res. Int."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"138375","DOI":"10.1016\/j.jcis.2025.138375","article-title":"Polymorphism on the interfacial adhesion of amyloid-like fibrils: Insights from assembly units and secondary structures","volume":"700","author":"Guo","year":"2025","journal-title":"J. Colloid Interface Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.bbapap.2014.12.020","article-title":"Structural characterization of amyloid fibrils from the human parathyroid hormone","volume":"1854","author":"Gopalswamy","year":"2015","journal-title":"Biochim. Biophys. Acta (BBA)-Proteins Proteom."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"C363","DOI":"10.1111\/j.1365-2621.2004.tb10698.x","article-title":"Selective proteolysis of the glycinin and \u03b2-conglycinin fractions in a soy protein isolate by pepsin and papain with controlled pH and temperature","volume":"69","author":"Tsumura","year":"2004","journal-title":"J. Food Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1111\/ijfs.12482","article-title":"Effects of pepsin hydrolysis on the soy \u03b2-conglycinin aggregates formed by heat treatment at different pH","volume":"49","author":"He","year":"2014","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4384","DOI":"10.1039\/C7FO00812K","article-title":"Soy peptide aggregates formed during hydrolysis reduced protein extraction without decreasing their nutritional value","volume":"8","author":"Zhang","year":"2017","journal-title":"Food Funct."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"107615","DOI":"10.1016\/j.foodhyd.2022.107615","article-title":"Evaluation of the impact of stirring on the formation, structural changes and rheological properties of ovalbumin fibrils","volume":"128","author":"Wang","year":"2022","journal-title":"Food Hydrocoll."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.supflu.2018.04.006","article-title":"Production of \u03b2-carotene nanosuspensions using supercritical CO2 and improvement of its efficiency by Z-isomerization pre-treatment","volume":"138","author":"Ono","year":"2018","journal-title":"J. Supercrit. Fluids"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"144176","DOI":"10.1016\/j.foodchem.2025.144176","article-title":"Research on the release and absorption regularities of free amino acids and peptides in vitro digestion of yeast protein","volume":"482","author":"Qiao","year":"2025","journal-title":"Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"111569","DOI":"10.1016\/j.jfoodeng.2023.111569","article-title":"Hindering interparticle agglomeration of \u03b2-carotene by wall material complexation at the solid-liquid interface","volume":"355","author":"Zhang","year":"2023","journal-title":"J. Food Eng."}],"container-title":["Nanomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4991\/15\/23\/1829\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T05:23:48Z","timestamp":1764912228000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4991\/15\/23\/1829"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,3]]},"references-count":44,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["nano15231829"],"URL":"https:\/\/doi.org\/10.3390\/nano15231829","relation":{},"ISSN":["2079-4991"],"issn-type":[{"value":"2079-4991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,3]]}}}