{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T05:24:20Z","timestamp":1787117060489,"version":"build-2736575974"},"reference-count":80,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","award":["PID2023-151881OB-I00"],"award-info":[{"award-number":["PID2023-151881OB-I00"]}]},{"award":["PID2023-151881OB-I00"],"award-info":[{"award-number":["PID2023-151881OB-I00"]}],"id":[{"id":"https:\/\/ror.org\/05r0vyz12","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006393","name":"Universidad de Granada","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006393","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/04njjy449","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>Magnetopolymeric nanocomposites represent promising platforms for advanced nanomedicine due to their ability to combine magnetic responsiveness with polymer-mediated biological functionality. In this study, a hybrid nanocomposite system based on maghemite (Mh), poly(ethyl cyanoacrylate) (PECA), and chitosan (Cs) was developed and systematically characterized for biomedical applications. Mh nanoparticles (NPs) were incorporated as magnetic cores, while PECA acted as a biodegradable polymeric matrix, and chitosan provided surface functionalization and enhanced biocompatibility. The nanocomposites were prepared following anionic polymerization and coacervation methods and characterized in terms of structure particle size, electrokinetics and magnetic responsiveness. The results demonstrated the formation of a nanoscale (core\/shell)\/shell system with superparamagnetic properties. Importantly, the nanocomposites were evaluated as magnetic resonance imaging (MRI) contrast agents, exhibiting significant transverse relaxivity. In vitro cytotoxicity assays demonstrated the absence of significant toxic effects, while ex vivo hemocompatibility studies confirmed their compatibility with blood components. Furthermore, their potential as hyperthermia agents was demonstrated in vitro under the influence of an alternating magnetic field (AMF). Overall, the (core@shell)@shell nanocomposites combined superparamagnetic functionalities with favorable biological properties, supporting their potential use as multifunctional platforms for theranostic applications, including MRI contrast enhancement and magnetically induced hyperthermia.<\/jats:p>","DOI":"10.3390\/nano16161013","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T10:04:36Z","timestamp":1786961076000},"page":"1013","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineering Chitosan-Functionalized Magnetopolymeric Nanoparticles as a Theranostic Platform for MRI-Guided Magnetic Hyperthermia"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9462-2834","authenticated-orcid":false,"given":"Silvia","family":"Fuerte-Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8275-7733","authenticated-orcid":false,"given":"Lorena","family":"Garc\u00eda-Hevia","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, University of Cantabria, 39005 Santander, Spain"},{"name":"Nanomedicine Group, Marqu\u00e9s de Valdecilla Research Institute (IDIVAL), Avda. Cardenal Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-3234","authenticated-orcid":false,"given":"Juan","family":"Gallo","sequence":"additional","affiliation":[{"name":"Advanced (Magnetic) Theranostic Nanostructures Lab, Nanomedicine Group, International Iberian Nanotechnology Laboratory (INL), Avda. Mestre Jos\u00e9 Veiga, 4715-330 Braga, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4319-2631","authenticated-orcid":false,"given":"Manuel","family":"Ba\u00f1obre-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Advanced (Magnetic) Theranostic Nanostructures Lab, Nanomedicine Group, International Iberian Nanotechnology Laboratory (INL), Avda. 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Clin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/s41392-023-01536-y","article-title":"Nanomedicine in Cancer Therapy","volume":"8","author":"Fan","year":"2023","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Bayda, S., Adeel, M., Tuccinardi, T., Cordani, M., and Rizzolio, F. (2019). The History of Nanoscience and Nanotechnology: From Chemical\u2013Physical Applications to Nanomedicine. Molecules, 25.","DOI":"10.3390\/molecules25010112"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.jconrel.2022.03.043","article-title":"Strategies to Improve the EPR Effect: A Mechanistic Perspective and Clinical Translation","volume":"345","author":"Wang","year":"2022","journal-title":"J. Control. Release"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1163\/156856189X00191","article-title":"Developments and Applications of Cyanoacrylate Adhesives","volume":"3","author":"Shantha","year":"1989","journal-title":"J. Adhes. Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jconrel.2003.08.005","article-title":"Drug Delivery to Resistant Tumors: The Potential of Poly(Alkyl Cyanoacrylate) Nanoparticles","volume":"93","author":"Vauthier","year":"2003","journal-title":"J. Control. Release"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1002\/ijc.30517","article-title":"Nanomedicine in the Management of Hepatocellular Carcinoma: Challenges and Opportunities","volume":"140","author":"Mohamed","year":"2017","journal-title":"Int. J. Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1186\/s12951-022-01615-2","article-title":"Emerging Nanobiotechnology for Precise Theranostics of Hepatocellular Carcinoma","volume":"20","author":"Xu","year":"2022","journal-title":"J. Nanobiotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"19436","DOI":"10.1021\/acsami.4c22386","article-title":"Superparamagnetic Superparticles for Magnetic Hyperthermia Therapy: Overcoming the Particle Size Limit","volume":"17","author":"Attanayake","year":"2025","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"134208","DOI":"10.1016\/j.colsurfa.2024.134208","article-title":"Enhancing the MRI Contrast and Hyperthermic Properties of Magnetic Nanoflowers by Modulating Magnetisation Dynamics Using Poly(Butyl Cyanoacrylate) Shells","volume":"695","author":"Moloney","year":"2024","journal-title":"Colloids Surf. Physicochem. Eng. Asp."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"790","DOI":"10.3109\/02656736.2013.822993","article-title":"Magnetic Nanoparticle Heating and Heat Transfer on a Microscale: Basic Principles, Realities and Physical Limitations of Hyperthermia for Tumour Therapy","volume":"29","author":"Dutz","year":"2013","journal-title":"Int. J. Hyperth."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"R167","DOI":"10.1088\/0022-3727\/36\/13\/201","article-title":"Applications of Magnetic Nanoparticles in Biomedicine","volume":"36","author":"Pankhurst","year":"2003","journal-title":"J. Phys. D Appl. Phys."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.cis.2011.04.003","article-title":"Magnetic Fluid Hyperthermia: Focus on Superparamagnetic Iron Oxide Nanoparticles","volume":"166","author":"Laurent","year":"2011","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Luengo, Y., D\u00edaz-Riascos, Z.V., Garc\u00eda-Soriano, D., Teran, F.J., Art\u00e9s-Ib\u00e1\u00f1ez, E.J., Ibarrola, O., Somoza, \u00c1., Miranda, R., Schwartz, S., and Abasolo, I. (2022). Fine Control of in Vivo Magnetic Hyperthermia Using Iron Oxide Nanoparticles with Different Coatings and Degrees of Aggregation. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14081526"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.1021\/cr068445e","article-title":"Magnetic Iron Oxide Nanoparticles: Synthesis, Stabilization, Vectorization, Physicochemical Characterizations, and Biological Applications","volume":"108","author":"Laurent","year":"2008","journal-title":"Chem. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3445","DOI":"10.2147\/IJN.S30320","article-title":"Superparamagnetic Iron Oxide Nanoparticles: Magnetic Nanoplatforms as Drug Carriers","volume":"7","author":"Wahajuddin","year":"2012","journal-title":"Int. J. Nanomed."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4607","DOI":"10.1021\/nl102623x","article-title":"Coating Optimization of Superparamagnetic Iron Oxide Nanoparticles for High T2 Relaxivity","volume":"10","author":"Tong","year":"2010","journal-title":"Nano Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2575","DOI":"10.1039\/C1CS15248C","article-title":"Designed Synthesis of Uniformly Sized Iron Oxide Nanoparticles for Efficient Magnetic Resonance Imaging Contrast Agents","volume":"41","author":"Lee","year":"2012","journal-title":"Chem. Soc. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"103166","DOI":"10.1016\/j.cis.2024.103166","article-title":"Advances in Surface Design and Biomedical Applications of Magnetic Nanoparticles","volume":"328","author":"Carneiro","year":"2024","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3042","DOI":"10.1208\/s12249-017-0792-3","article-title":"Development and Characterization of Magnetite\/Poly(Butyl Cyanoacrylate) Nanoparticles for Magnetic Targeted Drug Delivery","volume":"18","author":"Cabeza","year":"2017","journal-title":"AAPS PharmSciTech"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/s12929-025-01161-7","article-title":"Chitosan-Based Formulations for Therapeutic Applications: A Recent Overview","volume":"32","author":"Gonciarz","year":"2025","journal-title":"J. Biomed. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.carbpol.2013.01.072","article-title":"Comparative Study of Chitosan Shielding Effect on Nanocarrier Hydrophilicity and Biodistribution","volume":"94","author":"Ishak","year":"2013","journal-title":"Carbohydr. Polym."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Imran, H., Tang, Y., Wang, S., Yan, X., Liu, C., Guo, L., Wang, E., and Xu, C. (2024). Optimized Doxorubicin Delivery via Chitosan-Mediated Nanoparticles and Stimuli-Responsive Systems in Cancer Chemotherapy: A Review. Molecules, 29.","DOI":"10.3390\/molecules29010031"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s00339-024-07452-4","article-title":"Evaluating PVP Coated Iron Oxide Particles for Localized Magnetic Hyperthermia and MRI Imaging","volume":"130","author":"Orsini","year":"2024","journal-title":"Appl. Phys. A"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"9057","DOI":"10.1039\/D5DT00609K","article-title":"Iron Oxide Nanospheres: Dual Functionality as MRI Contrast Agents and Magnetic Fluid Hyperthermia Therapeutics","volume":"54","author":"Porru","year":"2025","journal-title":"Dalton Trans."},{"key":"ref_26","first-page":"245","article-title":"Imaging-Guided Precision Hyperthermia with Magnetic Nanoparticles","volume":"3","author":"Bulte","year":"2025","journal-title":"Nat. Rev. Bioeng."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3103","DOI":"10.1002\/pol.20230820","article-title":"Polymer\/Iron Oxide Nanocomposites as Magnetic Resonance Imaging Contrast Agents: Polymer Modulation and Probe Property Control","volume":"62","author":"Gu","year":"2024","journal-title":"J. Polym. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"de la Harpe, K.M., Kondiah, P.P.D., Choonara, Y.E., Marimuthu, T., du Toit, L.C., and Pillay, V. (2019). The Hemocompatibility of Nanoparticles: A Review of Cell\u2013Nanoparticle Interactions and Hemostasis. Cells, 8.","DOI":"10.3390\/cells8101209"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1109\/TMAG.1981.1061188","article-title":"Preparation of Aqueous Magnetic Liquids in Alkaline and Acidic Media","volume":"17","author":"Massart","year":"1981","journal-title":"IEEE Trans. Magn."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1002\/wnan.15","article-title":"Synthesis of Poly(Alkyl Cyanoacrylate)-Based Colloidal Nanomedicines","volume":"1","author":"Nicolas","year":"2009","journal-title":"WIREs Nanomed. Nanobiotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1002\/(SICI)1097-4628(19970103)63:1<125::AID-APP13>3.0.CO;2-4","article-title":"Novel Hydrophilic Chitosan\u2013Polyethylene Oxide Nanoparticles as Protein Carriers","volume":"63","author":"Calvo","year":"1997","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.colsurfb.2005.06.001","article-title":"Modulation of Surface Charge, Particle Size and Morphological Properties of Chitosan\u2013TPP Nanoparticles Intended for Gene Delivery","volume":"44","author":"Gan","year":"2005","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.jconrel.2004.08.010","article-title":"Recent Advances on Chitosan-Based Micro- and Nanoparticles in Drug Delivery","volume":"100","author":"Agnihotri","year":"2004","journal-title":"J. Control. Release"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.jconrel.2007.09.008","article-title":"Tegafur Loading and Release Properties of Magnetite\/Poly(Alkylcyanoacrylate) (Core\/Shell) Nanoparticles","volume":"125","author":"Arias","year":"2008","journal-title":"J. Control. Release Off. J. Control. Release Soc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0168-3659(01)00519-3","article-title":"Synthesis and Characterization of Poly(Ethyl-2-Cyanoacrylate) Nanoparticles with a Magnetic Core","volume":"77","author":"Arias","year":"2001","journal-title":"J. Control. Release Off. J. Control. Release Soc."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.jcis.2006.03.005","article-title":"Preparation and Characterization of Carbonyl Iron\/Poly(Butylcyanoacrylate) Core\/Shell Nanoparticles","volume":"299","author":"Arias","year":"2006","journal-title":"J. Colloid Interface Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4218","DOI":"10.1039\/C9RA10857B","article-title":"PLGA Nanoparticle Preparations by Emulsification and Nanoprecipitation Techniques: Effects of Formulation Parameters","volume":"10","year":"2020","journal-title":"RSC Adv."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Mahmoud, B.S., and McConville, C. (2025). Formulation and Systematic Optimisation of Polymeric Blend Nanoparticles via Box\u2013Behnken Design. Pharmaceutics, 17.","DOI":"10.3390\/pharmaceutics17101351"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"11614","DOI":"10.1039\/D1CS00427A","article-title":"Magnetic Nanoparticles and Clusters for Magnetic Hyperthermia: Optimizing Their Heat Performance and Developing Combinatorial Therapies to Tackle Cancer","volume":"50","author":"Avugadda","year":"2021","journal-title":"Chem. Soc. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5158","DOI":"10.1021\/acsabm.5c00501","article-title":"Enhanced Hyperthermic Response of Dextrose-Coated Mn0.5Zn0.5Fe2O4 Nanorings Containing Magnetic Fluid and Its Cytotoxicity and Uptake in HeLa Cells","volume":"8","author":"Patel","year":"2025","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s00418-024-02283-z","article-title":"Increased Endocytosis Rate and Enhanced Lysosomal Pathway of Silica-Coated Superparamagnetic Nanoparticles into M-HeLa Cells Compared with Cultured Primary Motor Neurons","volume":"161","author":"Sibgatullina","year":"2024","journal-title":"Histochem. Cell Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ijpharm.2015.01.029","article-title":"Carbon Encapsulated Iron Oxide Nanoparticles Surface Engineered with Polyethylene Glycol-Folic Acid to Induce Selective Hyperthermia in Folate over Expressed Cancer Cells","volume":"480","author":"Sadhasivam","year":"2015","journal-title":"Int. J. Pharm."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jmmm.2006.10.1156","article-title":"Magnetic Particle Hyperthermia\u2014Biophysical Limitations of a Visionary Tumour Therapy","volume":"311","author":"Hergt","year":"2007","journal-title":"J. Magn. Magn. Mater."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1118\/1.595376","article-title":"Temperature Distributions in Hyperthermia by Electromagnetic Induction: A Theoretical Model for the Thorax","volume":"10","author":"Brezovich","year":"1983","journal-title":"Med. Phys."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Weber, M., Steinle, H., Golombek, S., Hann, L., Schlensak, C., Wendel, H.P., and Avci-Adali, M. (2018). Blood-Contacting Biomaterials: In Vitro Evaluation of the Hemocompatibility. Front. Bioeng. Biotechnol., 6.","DOI":"10.3389\/fbioe.2018.00099"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"25518","DOI":"10.1038\/srep25518","article-title":"An Evaluation of Blood Compatibility of Silver Nanoparticles","volume":"6","author":"Chen","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2180","DOI":"10.1021\/nl0805615","article-title":"Method for Analysis of Nanoparticle Hemolytic Properties in Vitro","volume":"8","author":"Dobrovolskaia","year":"2008","journal-title":"Nano Lett."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.jconrel.2016.06.017","article-title":"DLS and Zeta Potential\u2013What They Are and What They Are Not?","volume":"235","author":"Bhattacharjee","year":"2016","journal-title":"J. Control. Release"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Danaei, M., Dehghankhold, M., Ataei, S., Hasanzadeh Davarani, F., Javanmard, R., Dokhani, A., Khorasani, S., and Mozafari, M.R. (2018). Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems. Pharmaceutics, 10.","DOI":"10.3390\/pharmaceutics10020057"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"16014","DOI":"10.1038\/natrevmats.2016.14","article-title":"Analysis of Nanoparticle Delivery to Tumours","volume":"1","author":"Wilhelm","year":"2016","journal-title":"Nat. Rev. Mater."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1038\/s44222-023-00138-1","article-title":"Controlling the Biodistribution and Clearance of Nanomedicines","volume":"2","author":"Cabral","year":"2024","journal-title":"Nat. Rev. Bioeng."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1042\/bj20031253","article-title":"Size-Dependent Internalization of Particles via the Pathways of Clathrin- and Caveolae-Mediated Endocytosis","volume":"377","author":"Rejman","year":"2004","journal-title":"Biochem. J."},{"key":"ref_53","first-page":"311","article-title":"Nanomedicine: Current Status and Future Prospects","volume":"19","author":"Moghimi","year":"2005","journal-title":"FASEB J. Off. Publ. Fed. Am. Soc. Exp. Biol."},{"key":"ref_54","unstructured":"Silverstein, R.M., and Webster, F.X. (1998). Spectrometric Identification of Organic Compounds, Wiley. [6th ed.]."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1039\/D1BM01963E","article-title":"Superparamagnetic Iron Oxide Nanoparticles for Magnetic Hyperthermia: Recent Advancements, Molecular Effects, and Future Directions in the Omics Era","volume":"10","author":"Pucci","year":"2022","journal-title":"Biomater. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"100875","DOI":"10.1016\/j.jsamd.2025.100875","article-title":"Iron Oxide Nanoparticles Enhancing Magnetic Resonance Imaging: A Review of the Latest Advancements","volume":"10","author":"Aboushoushah","year":"2025","journal-title":"J. Sci. Adv. Mater. Devices"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"106054","DOI":"10.1016\/j.ebiom.2025.106054","article-title":"Superparamagnetic Iron Oxide Nanoparticles in Clinical Applications: Current Status and Future Perspectives","volume":"82","author":"Guo","year":"2025","journal-title":"eBioMedicine"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"224001","DOI":"10.1088\/0022-3727\/42\/22\/224001","article-title":"Progress in Applications of Magnetic Nanoparticles in Biomedicine","volume":"42","author":"Pankhurst","year":"2009","journal-title":"J. Phys. D Appl. Phys."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"582","DOI":"10.33263\/BRIAC136.582","article-title":"Assessment of the Functionalization of Chitosan\/Iron Oxide Nanoparticles","volume":"13","author":"Ismail","year":"2023","journal-title":"Biointerface Res. Appl. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1002\/anie.200602866","article-title":"Magnetic Nanoparticles: Synthesis, Protection, Functionalization, and Application","volume":"46","author":"Lu","year":"2007","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"6965","DOI":"10.1039\/D0NR08615K","article-title":"Mechanism of Magnetization Reduction in Iron Oxide Nanoparticles","volume":"13","author":"Feoktystov","year":"2021","journal-title":"Nanoscale"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/978-1-4939-6840-4_4","article-title":"Magnetic Characterization of Iron Oxide Nanoparticles for Biomedical Applications","volume":"Volume 1570","author":"Petrosko","year":"2017","journal-title":"Biomedical Nanotechnology"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"10935","DOI":"10.1021\/nn304477s","article-title":"Cooperative Organization in Iron Oxide Multi-Core Nanoparticles Potentiates Their Efficiency as Heating Mediators and MRI Contrast Agents","volume":"6","author":"Lartigue","year":"2012","journal-title":"ACS Nano"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1038\/nm1467","article-title":"Artificially Engineered Magnetic Nanoparticles for Ultra-Sensitive Molecular Imaging","volume":"13","author":"Lee","year":"2007","journal-title":"Nat. Med."},{"key":"ref_65","first-page":"214","article-title":"Recent Advances in Engineering Iron Oxide Nanoparticles for Effective Magnetic Resonance Imaging","volume":"12","author":"Zhao","year":"2021","journal-title":"Bioact. Mater."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"131673","DOI":"10.1016\/j.matchemphys.2025.131673","article-title":"Relaxivity and Cytotoxicity of Iron Oxide Nanoparticles with Different Coatings for Contrast Agents in Magnetic Resonance Imaging","volume":"348","author":"Vargas","year":"2026","journal-title":"Mater. Chem. Phys."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.7150\/thno.23789","article-title":"Surface Impact on Nanoparticle-Based Magnetic Resonance Imaging Contrast Agents","volume":"8","author":"Zhang","year":"2018","journal-title":"Theranostics"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"5681","DOI":"10.1016\/j.biomaterials.2004.01.023","article-title":"Biomaterial-Associated Thrombosis: Roles of Coagulation Factors, Complement, Platelets and Leukocytes","volume":"25","author":"Gorbet","year":"2004","journal-title":"Biomaterials"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"408","DOI":"10.2174\/0929867323666160106151428","article-title":"Action of Nanoparticles on Platelet Activation and Plasmatic Coagulation","volume":"23","year":"2016","journal-title":"Curr. Med. Chem."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Luna-V\u00e1zquez-G\u00f3mez, R., Arellano-Garc\u00eda, M.E., Garc\u00eda-Ramos, J.C., Radilla-Ch\u00e1vez, P., Salas-Vargas, D.S., Casillas-Figueroa, F., Ruiz-Ruiz, B., Bogdanchikova, N., and Pestryakov, A. (2021). Hemolysis of Human Erythrocytes by ArgovitTM AgNPs from Healthy and Diabetic Donors: An In Vitro Study. Materials, 14.","DOI":"10.3390\/ma14112792"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"4963","DOI":"10.1039\/D1TB00354B","article-title":"Engineering of Stealth (Maghemite\/PLGA)\/Chitosan (Core\/Shell)\/Shell Nanocomposites with Potential Applications for Combined MRI and Hyperthermia against Cancer","volume":"9","author":"Caro","year":"2021","journal-title":"J. Mater. Chem. B"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1039\/D4NR03034F","article-title":"The Interaction of Glycogen Nanoparticles with Human Blood","volume":"17","author":"Davydiuk","year":"2025","journal-title":"Nanoscale"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"10530","DOI":"10.1038\/s41598-020-67207-y","article-title":"Efficient Uptake and Retention of Iron Oxide-Based Nanoparticles in HeLa Cells Leads to an Effective Intracellular Delivery of Doxorubicin","volume":"10","author":"Popescu","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"3161","DOI":"10.1016\/j.biomaterials.2008.04.016","article-title":"Universal Cell Labelling with Anionic Magnetic Nanoparticles","volume":"29","author":"Wilhelm","year":"2008","journal-title":"Biomaterials"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/S0304-8853(02)00706-0","article-title":"Heating Magnetic Fluid with Alternating Magnetic Field","volume":"252","author":"Rosensweig","year":"2002","journal-title":"J. Magn. Magn. Mater."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"452001","DOI":"10.1088\/0957-4484\/25\/45\/452001","article-title":"Magnetic Particle Hyperthermia\u2014A Promising Tumour Therapy?","volume":"25","author":"Dutz","year":"2014","journal-title":"Nanotechnology"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"3080","DOI":"10.1021\/nn2048137","article-title":"Water-Soluble Iron Oxide Nanocubes with High Values of Specific Absorption Rate for Cancer Cell Hyperthermia Treatment","volume":"6","author":"Guardia","year":"2012","journal-title":"ACS Nano"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1021\/ja067457e","article-title":"Size-Sorted Anionic Iron Oxide Nanomagnets as Colloidal Mediators for Magnetic Hyperthermia","volume":"129","author":"Fortin","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Vilas-Boas, V., Carvalho, F., and Espi\u00f1a, B. (2020). Magnetic Hyperthermia for Cancer Treatment: Main Parameters Affecting the Outcome of In Vitro and In Vivo Studies. Molecules, 25.","DOI":"10.3390\/molecules25122874"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s11060-006-9195-0","article-title":"Intracranial Thermotherapy Using Magnetic Nanoparticles Combined with External Beam Radiotherapy: Results of a Feasibility Study on Patients with Glioblastoma Multiforme","volume":"81","author":"Rothe","year":"2007","journal-title":"J. Neurooncol."}],"container-title":["Nanomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4991\/16\/16\/1013\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T04:29:08Z","timestamp":1787113748000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4991\/16\/16\/1013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":80,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["nano16161013"],"URL":"https:\/\/doi.org\/10.3390\/nano16161013","relation":{},"ISSN":["2079-4991"],"issn-type":[{"value":"2079-4991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]}}}