{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T01:45:16Z","timestamp":1778895916527,"version":"3.51.4"},"reference-count":31,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2016,3,16]],"date-time":"2016-03-16T00:00:00Z","timestamp":1458086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>Manufactured nanoparticles (NPs) are increasingly being used for commercial purposes and certain NP types have been shown to have broad spectrum antibacterial activity. In contrast, their activities against fungi and fungi-like oomycetes are less studied. Here, we examined the potential of two types of commercially available Zn NPs (Zn NPs and ZnO NPs) to inhibit spore germination and infectivity on tobacco leaves resulting from exposure to the fungi-like oomycete pathogen Peronospora tabacina (P. tabacina). Both types of NPs, as well as ZnCl2 and bulk ZnO control treatments, inhibited spore germination compared to a blank control. ZnO ENMs were shown to be a much more powerful suppressor of spore germination and infectivity than bulk ZnO. ZnO and Zn NPs significantly inhibited leaf infection at 8 and 10 mg\u00b7L\u22121, respectively. Both types of NPs were found to provide substantially higher concentration dependent inhibition of spore germination and infectivity than could be readily explained by the presence of dissolved Zn. These results suggest that both NP types have potential for use as economic, low-dose, potentially non-persistent anti-microbial agents against the oomycete P. tabacina.<\/jats:p>","DOI":"10.3390\/nano6030050","type":"journal-article","created":{"date-parts":[[2016,3,16]],"date-time":"2016-03-16T11:26:45Z","timestamp":1458127605000},"page":"50","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Nanoparticles Composed of Zn and ZnO Inhibit Peronospora tabacina Spore Germination in vitro and P. tabacina Infectivity on Tobacco Leaves"],"prefix":"10.3390","volume":"6","author":[{"given":"George","family":"Wagner","sequence":"first","affiliation":[{"name":"Department of Plant and Soil Sciences, University of Kentucky, Lexington 40546, KY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Korenkov","sequence":"additional","affiliation":[{"name":"Department of Plant and Soil Sciences, University of Kentucky, Lexington 40546, KY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Judy","sequence":"additional","affiliation":[{"name":"Department of Plant and Soil Sciences, University of Kentucky, Lexington 40546, KY, USA"},{"name":"CSIRO Land and Water, Urrbrae 5064, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Bertsch","sequence":"additional","affiliation":[{"name":"Department of Plant and Soil Sciences, University of Kentucky, Lexington 40546, KY, USA"},{"name":"CSIRO Land and Water Flagship, CSIRO, Dutton Park 4102, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,3,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1038\/nrmicro3028","article-title":"Antimicrobial activity of metals: Molecular targets and applications","volume":"11","author":"Lemire","year":"2013","journal-title":"Nat. Rev. Mircrobiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6003","DOI":"10.2147\/IJN.S35347","article-title":"Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: A comparative study","volume":"7","author":"Azam","year":"2012","journal-title":"Int. J. Nano Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1021\/nl052326h","article-title":"Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium","volume":"6","author":"Brayner","year":"2006","journal-title":"Nano Lett."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1897\/08-090.1","article-title":"Nanomaterials in the environment: Behavior, fate, bioavailability and effects","volume":"27","author":"Klaine","year":"2008","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8484","DOI":"10.1021\/es071445r","article-title":"Comparative toxicity of nanoparticulate ZnO, bulk ZnO, and ZnCl2 to a freshwater microalga (Pseudokirchneriella subcapita): The importance of particle solubility","volume":"41","author":"Franklin","year":"2007","journal-title":"Environ. Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"5580","DOI":"10.1021\/es800422x","article-title":"Root uptake and phytotoxicity of ZnO nanoparticles","volume":"42","author":"Lin","year":"2008","journal-title":"Environ. Sci. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1007\/s10646-008-0214-0","article-title":"Environmental behavior and ecotoxicity of engineered nanoparticles to algae, plants, and fungi","volume":"17","author":"Navarro","year":"2008","journal-title":"Ecotoxicology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"9473","DOI":"10.1021\/es901695c","article-title":"Assay-dependent phytoxicity of nanoparticles to plants","volume":"43","author":"Stampoulis","year":"2009","journal-title":"Environ. Sci. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2852","DOI":"10.1016\/j.scitotenv.2011.03.022","article-title":"Effects of ZnO particles on activated sludge: Role of particle dissolution","volume":"409","author":"Liu","year":"2011","journal-title":"Sci. Total Environ."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.colsurfa.2014.05.057","article-title":"The contribution of zinc ions to the antimicrobial activity of zinc oxide","volume":"457","author":"Pasquet","year":"2014","journal-title":"Colloids Surf. A"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Lipovsky, A., Nitzan, Y., Gedanken, A., and Lubart, R. (2011). Antifungal activity of ZnO nanoparticles\u2014The role of ROS mediated cell injury. Nanotechnology, 22.","DOI":"10.1088\/0957-4484\/22\/10\/105101"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1745-4565.2010.00287.x","article-title":"Antibacterial action of zinc oxide nanoparticles against foodborne pathogens","volume":"31","author":"Tayel","year":"2011","journal-title":"J. Food Saf."},{"key":"ref_13","unstructured":"Sparks, D. (2014). Bioavailability, Toxicity and Fate of Manufactured Nanomaterials in Terrestrial Ecosystems, Elsevier."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.envpol.2007.01.016","article-title":"Phytotoxicity of nanoparticles: Inhibition of seed germination and root growth","volume":"150","author":"Lin","year":"2007","journal-title":"Environ. Pollut."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7315","DOI":"10.1021\/es903891g","article-title":"Evidence of the differential biotransformation and genotoxicity of ZnO and CeO2 nanoparticles on soybean (Glycine max) Plants","volume":"44","author":"Botez","year":"2010","journal-title":"Environ. Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.1016\/j.scitotenv.2010.03.031","article-title":"Interactions between engineered nanoparticles (ENPs) and plants: Phytoxicity, uptake and accumulation","volume":"408","author":"Ma","year":"2010","journal-title":"Sci. Total Environ."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4842","DOI":"10.3390\/ma3104842","article-title":"Toxicity of transition metal oxide nanoparticles: Recent insights from in vitro studies","volume":"3","author":"Huang","year":"2010","journal-title":"Materials"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1039\/c0em00611d","article-title":"TiO2 and ZnO nanoparticles negatively affect wheat growth and soil enzyme activities in agricultrual soil","volume":"13","author":"Du","year":"2011","journal-title":"J. Environ. Monit."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1146\/annurev.phyto.34.1.549","article-title":"Resistance to phenylamide fungicides: A case study with Phytophthora infestans involving mating type and race structure","volume":"34","author":"Gisi","year":"1996","journal-title":"Ann. Rev. Phytopathol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1006\/fgbi.1998.1107","article-title":"Genetics of metalaxyl resistance in Phytophthora infestans","volume":"26","author":"Lee","year":"1999","journal-title":"Fungal Genet. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1104\/pp.107.097584","article-title":"Impacts of T-phylloplanin gene knockdown and of Heilanthus and Datura phylloplanins on Personospora tabacina spore germination and disease potential","volume":"144","author":"Kroumova","year":"2007","journal-title":"Plant Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.micres.2010.03.003","article-title":"Antifungal activity of zinc oxide nanoparticles against Botrytis cinerea and Penicillium expansum","volume":"166","author":"He","year":"2011","journal-title":"Microbol. Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Weast, R.C. (1969). Handbook of Chemistry and Physics, The Chemical Rubber Co.","DOI":"10.1097\/00000441-196906000-00020"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4757","DOI":"10.1021\/es404544w","article-title":"Multitechnique investigation of the pH dependence of phosphate induced transformations of ZnO nanoparticles","volume":"48","author":"Rathnayake","year":"2014","journal-title":"Environ. Sci. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2346","DOI":"10.1088\/0957-4484\/16\/10\/059","article-title":"The bactericidal effect of silver nanoparticles","volume":"16","author":"Morones","year":"2005","journal-title":"Nanotechnology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1128\/aem.36.6.906-914.1978","article-title":"Toxicity of zinc to fungi, bacteria, and coliphages: Influence of chloride ions","volume":"36","author":"Babich","year":"1978","journal-title":"Appl. Environ. Micrrobil."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/j.1462-5822.2006.00833.x","article-title":"Cell biology of plant-oomycete interactions","volume":"9","author":"Hardham","year":"2007","journal-title":"Cell. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8759","DOI":"10.1021\/acs.est.5b01211","article-title":"Toxicogenomic responses of the model legume Medicago truncatula to aged biosolids containing a mixture of nanomaterials (TiO2, Ag and ZnO) from a pilot wastewater treatment plant","volume":"49","author":"Chen","year":"2015","journal-title":"Environ. Sci. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"8751","DOI":"10.1021\/acs.est.5b01208","article-title":"Nanomaterials in biosolids inhibit nodulation, shift microbial community composition, and result in increased metal uptake relative to bulk metals","volume":"49","author":"Judy","year":"2015","journal-title":"Environ. Sci. Technol."},{"key":"ref_30","first-page":"1092","article-title":"Remocal of duvatrienes from the surface of tobacco leaves increases their susceptibility to blue mold","volume":"76","author":"Reuveni","year":"1987","journal-title":"Phytopathology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1105\/tpc.105.031559","article-title":"Phylloplanins of tobacco are defensive proteins deployed on aerial surfaces by short glandular trichomes","volume":"17","author":"Shepherd","year":"2005","journal-title":"Plant Cell"}],"container-title":["Nanomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4991\/6\/3\/50\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:20:49Z","timestamp":1760210449000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4991\/6\/3\/50"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,16]]},"references-count":31,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2016,3]]}},"alternative-id":["nano6030050"],"URL":"https:\/\/doi.org\/10.3390\/nano6030050","relation":{},"ISSN":["2079-4991"],"issn-type":[{"value":"2079-4991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,16]]}}}