{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:44:40Z","timestamp":1789865080672,"version":"4.0.1"},"reference-count":29,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2017,8,13]],"date-time":"2017-08-13T00:00:00Z","timestamp":1502582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>This paper describes the evaluation of polymeric nanoparticles (NPs) as a potential carrier for lung administration of fluticasone propionate (FP). The chosen polymeric material to produce NPs was a copolymer based on \u03b1,\u03b2-poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) whose backbone was derivatised with different molecules, such as poly(lactic acid) (PLA) and polyethylenglycol (PEG). The chosen method to produce NPs from PHEA-PLA-PEG2000 was the method based on high-pressure homogenization and subsequent solvent evaporation by adding Pluronic F68 during the process and trehalose before lyophilisation. Obtained colloidal FP-loaded NPs showed a slightly negative surface charge and nanometric dimensions that are maintained after storage for one year at \u221220 \u00b0C and 5 \u00b0C. The FP loading was about 2.9 wt % and the drug was slowly released in simulated lung fluid. Moreover, the obtained NPs, containing the drug or not, were biocompatible and did not induce cell necrosis and cell apoptosis on bronchial epithelial cells (16-HBE). Further in vitro testing on cigarette smoke extract (CSE)-stimulated 16-HBE revealed that FP-loaded NPs were able to reduce the survivin expression, while either free FP or empty NPs were not able to significantly reduce this effect.<\/jats:p>","DOI":"10.3390\/nano7080222","type":"journal-article","created":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T10:23:12Z","timestamp":1502706192000},"page":"222","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Polyaspartamide-Based Nanoparticles Loaded with Fluticasone Propionate and the In Vitro Evaluation towards Cigarette Smoke Effects"],"prefix":"10.3390","volume":"7","author":[{"given":"Emanuela","family":"Craparo","sequence":"first","affiliation":[{"name":"Laboratory of Biocompatible Polymers, Dipartimento di Scienze e Tecnologie, Biologiche, Chimiche e Farmaceutiche (STEBICEF), Universit\u00e0 di Palermo, via Archirafi 32, 90123 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Ferraro","sequence":"additional","affiliation":[{"name":"Istituto di Biomedicina e Immunologia Molecolare, Consiglio Nazionale delle Ricerche-via Ugo La Malfa 153, 90146 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Pace","sequence":"additional","affiliation":[{"name":"Istituto di Biomedicina e Immunologia Molecolare, Consiglio Nazionale delle Ricerche-via Ugo La Malfa 153, 90146 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Bond\u00ec","sequence":"additional","affiliation":[{"name":"Istituto per lo Studio dei Materiali Nanostrutturati-U.O.S. di Palermo, Consiglio Nazionale delle Ricerche-via Ugo La Malfa 153, 90146 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaetano","family":"Giammona","sequence":"additional","affiliation":[{"name":"Laboratory of Biocompatible Polymers, Dipartimento di Scienze e Tecnologie, Biologiche, Chimiche e Farmaceutiche (STEBICEF), Universit\u00e0 di Palermo, via Archirafi 32, 90123 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gennara","family":"Cavallaro","sequence":"additional","affiliation":[{"name":"Laboratory of Biocompatible Polymers, Dipartimento di Scienze e Tecnologie, Biologiche, Chimiche e Farmaceutiche (STEBICEF), Universit\u00e0 di Palermo, via Archirafi 32, 90123 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,8,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.ijpharm.2015.07.008","article-title":"Formulation and Nebulization of Fluticasone Propionate-Loaded Lipid Nanocarriers","volume":"493","author":"Umerska","year":"2015","journal-title":"Int. J. Pharm."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1517\/17425247.2015.993968","article-title":"Pulmonary Delivery of Anti-Inflammatory Agents","volume":"12","author":"Ali","year":"2015","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1183\/09031936.00074905","article-title":"Relevance of Pharmacokinetics and Pharmacodynamics of Inhaled Corticosteroids to Asthma","volume":"28","author":"Derendorf","year":"2006","journal-title":"Eur. Respir. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.ijpharm.2016.02.031","article-title":"Enhanced Pulmonary Delivery of Fluticasone Propionate in Rodents by Mucus-Penetrating Nanoparticles","volume":"502","author":"Popov","year":"2016","journal-title":"Int. J. Pharm."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.jconrel.2014.01.007","article-title":"Lung Gene Therapy with Highly Compacted DNA Nanoparticles That Overcome the Mucus Barrier","volume":"178","author":"Suk","year":"2014","journal-title":"J. Control. Release"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.jconrel.2011.08.031","article-title":"Highly Compacted DNA Nanoparticles with Low MW PEG Coatings: In Vitro, Ex Vivo and in Vivo Evaluation","volume":"157","author":"Boylan","year":"2012","journal-title":"J. Control. Release"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1023\/A:1012248721523","article-title":"Biodistribution of Long-Circulating PEG-Grafted Nanocapsules in Mice: Effects of PEG Chain Length and Density","volume":"18","author":"Mosqueira","year":"2001","journal-title":"Pharm. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.pupt.2015.07.003","article-title":"Nanocomplexes for Gene Therapy of Respiratory Diseases: Targeting and Overcoming the Mucus Barrier","volume":"34","author":"Trapani","year":"2015","journal-title":"Pulm. Pharmacol. Ther."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1021\/acs.biomac.5b01480","article-title":"PegylatedPolyaspartamide\u2014Polylactide-Based Nanoparticles Penetrating Cystic Fibrosis Artificial Mucus","volume":"17","author":"Craparo","year":"2016","journal-title":"Biomacromolecules"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.1002\/pola.1987.080251016","article-title":"Reaction of \u03b1,\u03b2-poly(N-Hydroxyethyl)-dl-Aspartamide with Derivatives of Carboxylic Acids","volume":"25","author":"Giammona","year":"1987","journal-title":"J. Polym. Sci. Part A Polym. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4871","DOI":"10.1016\/S0032-3861(03)00486-5","article-title":"Molecular Characterization of \u03b1,\u03b2-poly(N-2-Hydroxyethyl)-dl-Aspartamide Derivatives as Potential Self-Assembling Copolymers Forming Polymeric Micelles","volume":"44","author":"Mendichi","year":"2003","journal-title":"Polymer"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3083","DOI":"10.1021\/bm060570c","article-title":"PEGylated Nanoparticles Based on a Polyaspartamide. Preparation, Physico-Chemical Characterization, and Intracellular Uptake","volume":"7","author":"Craparo","year":"2006","journal-title":"Biomacromolecules"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.ijpharm.2016.06.042","article-title":"Realization of Polyaspartamide-Based Nanoparticles and in Vivo Lung Biodistribution Evaluation of a Loaded Glucocorticoid after Aerosolization in Mice","volume":"510","author":"Craparo","year":"2016","journal-title":"Int. J. Pharm."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.ijpharm.2012.04.052","article-title":"PHEA-Graft-Polybutylmethacrylate Copolymer Microparticles for Delivery of Hydrophobic Drugs","volume":"433","author":"Licciardi","year":"2012","journal-title":"Int. J. Pharm."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1166\/jbn.2013.1608","article-title":"Novel Composed Galactosylated Nanodevices Containing a Ribavirin Prodrug as Hepatic Cell-Targeted Carriers for HCV Treatment","volume":"9","author":"Craparo","year":"2013","journal-title":"J. Biomed. Nanotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1002\/jbm.a.31316","article-title":"CrosslinkedHyaluronan with a Protein-like Polymer: Novel Bioresorbable Films for Biomedical Applications","volume":"84","author":"Pitarresi","year":"2008","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.polymdegradstab.2015.05.003","article-title":"Evaluation of Biodegradability on Polyaspartamide-Polylactic Acid Based Nanoparticles by Chemical Hydrolysis Studies","volume":"119","author":"Craparo","year":"2015","journal-title":"Polym. Degrad. Stab."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/j.ijpharm.2015.09.050","article-title":"PHEA-PLA Biocompatible Nanoparticles by Technique of Solvent Evaporation from Multiple Emulsions","volume":"495","author":"Cavallaro","year":"2015","journal-title":"Int. J. Pharm."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2629","DOI":"10.1007\/s11051-009-9842-4","article-title":"Nanoparticles Based on Novel Amphiphilic Polyaspartamide Copolymers","volume":"12","author":"Craparo","year":"2010","journal-title":"J. Nanopart. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1002\/macp.200400128","article-title":"Preparation of Polymeric Nanoparticles by Photo-Crosslinking of an AcryloylatedPolyaspartamide in w\/o Microemulsion","volume":"205","author":"Craparo","year":"2004","journal-title":"Macromol. Chem. Phys."},{"key":"ref_21","unstructured":"(2017, August 13). Stability Testing of New Drug Substances and Products Q1A (R2). Available online: https:\/\/urlsand.esvalabs.com\/?u=http%3A%2F%2Facademy.gmp-compliance.org%2Fguidemgr%2Ffiles%2FQ1A%28R2%29STEP4.PDF&e=541e9c83&h=6551e918&f=y&p=y."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1111\/j.1365-2567.2007.02788.x","article-title":"Cigarette smoke increases Toll-like receptor 4 and modify lipopolysaccharide-mediated responses in airway epithelial cells","volume":"124","author":"Pace","year":"2008","journal-title":"Immunology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1007\/s12192-013-0424-0","article-title":"Comparative cytoprotective effects of carbocysteine and fluticasone propionate in cigarette smoke extract-stimulated bronchial epithelial cells","volume":"18","author":"Pace","year":"2013","journal-title":"Cell Stress Chaperones"},{"key":"ref_24","first-page":"21","article-title":"Effects in Cigarette Smoke Stimulated Bronchial Epithelial Cells of a Corticosteroid Entrapped into Nanostructured Lipid Carriers","volume":"12","author":"Ferraro","year":"2014","journal-title":"J. Nanobiotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.pharmthera.2010.10.005","article-title":"At the Stem of Youth and Health","volume":"129","author":"Beltrami","year":"2011","journal-title":"Pharmacol. Ther."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.exger.2016.05.013","article-title":"Carbocysteine Counteracts the Effects of Cigarette Smoke on Cell Growth and on the SIRT1\/FoxO3 Axis in Bronchial Epithelial Cells","volume":"81","author":"Pace","year":"2016","journal-title":"Exp. Gerontol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"15","DOI":"10.14227\/DT180311P15","article-title":"Simulated Biologic Fluids with Possible Application in Dissolution Testing","volume":"18","author":"Marques","year":"2011","journal-title":"Dissolut. Technol."},{"key":"ref_28","first-page":"735","article-title":"A Transformed Human Epithelial Cell Line That Retains Tight Junctions Post Crisis. In Vitro","volume":"28","author":"Cozens","year":"1992","journal-title":"Cell. Dev. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1165\/ajrcmb.19.5.3091","article-title":"Effect of Cigarette Smoke Extract on Nitric Oxide Synthase in Pulmonary Artery Endothelial Cells","volume":"19","author":"Su","year":"1998","journal-title":"Am. J. Respir. Cell Mol. Biol."}],"container-title":["Nanomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4991\/7\/8\/222\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:42:15Z","timestamp":1760208135000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4991\/7\/8\/222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,13]]},"references-count":29,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2017,8]]}},"alternative-id":["nano7080222"],"URL":"https:\/\/doi.org\/10.3390\/nano7080222","relation":{},"ISSN":["2079-4991"],"issn-type":[{"value":"2079-4991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,13]]}}}