{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T13:39:11Z","timestamp":1776087551756,"version":"3.50.1"},"reference-count":53,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T00:00:00Z","timestamp":1538006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This work was financially supported by the National Centre for Research and Development","award":["LIDER\/ 154\/L-6\/14\/NCBR\/2015."],"award-info":[{"award-number":["LIDER\/ 154\/L-6\/14\/NCBR\/2015."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>The aim of this work was to investigate of biocompatibility of polymeric implants modified with silver nanoparticles (AgNPs). Middle ear prostheses (otoimplants) made of the (poly)acrylonitrile butadiene styrene (ABS) and ABS modified with silver nanoparticles were prepared through extrusion and injection moulding process. The obtained prostheses were characterized by SEM-EDX, micro-CT and mechanical tests, confirming their proper shape, good AgNPs homogenization and mechanical parameters stability. The biocompatibility of the implants was evaluated in vivo on rats, after 4, 12, 24 and 48 weeks of implantation. The tissue-healing process and cytotoxicity of the implants were evaluated on the basis of microscopic observations of the materials morphology after histochemical staining with cytochrome c oxidase (OCC) and acid phosphatase (AP), as well as via micro-tomography (ex vivo). The in vivo studies confirmed biocompatibility of the implants in the surrounding tissue environment. Both the pure ABS and nanosilver-modified ABS implants exhibited a distinct decrease in the area of granulation tissue which was replaced with the regenerating muscle tissue. Moreover, a slightly smaller area of granulation tissue was observed in the surroundings of the silver-doped prosthesis than in the case of pure ABS prosthesis. The kinetics of silver ions releasing from implants was investigated by ICP-MS spectrometry. The measurement confirmed that concentration of the silver ions increased within the implant\u2019s immersion period. Our results showed that middle ear implant with the nanoscale modification is biocompatible and might be used in ossicular reconstruction.<\/jats:p>","DOI":"10.3390\/nano8100764","type":"journal-article","created":{"date-parts":[[2018,9,28]],"date-time":"2018-09-28T02:54:54Z","timestamp":1538103294000},"page":"764","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Biocompatible Nanocomposite Implant with Silver Nanoparticles for Otology\u2014In Vivo Evaluation"],"prefix":"10.3390","volume":"8","author":[{"given":"Magdalena","family":"Zi\u0105bka","sequence":"first","affiliation":[{"name":"Department of Ceramics and Refractories, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30-059 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0602-3583","authenticated-orcid":false,"given":"El\u017cbieta","family":"Menaszek","sequence":"additional","affiliation":[{"name":"Department of Cytobiology, Collegium Medicum, Faculty of Pharmacy, UJ Jagiellonian University, 30-001 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacek","family":"Tarasiuk","sequence":"additional","affiliation":[{"name":"Department of Condensed Matter Physics, Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, 30-059 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Wro\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Condensed Matter Physics, Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, 30-059 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1177\/019459988409200515","article-title":"TORPs and PORPs: Causes of failure\u2014A report on 446 operations","volume":"92","author":"Sheehy","year":"1984","journal-title":"Otolaryngol. Head Neck Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1097\/00129492-200105000-00005","article-title":"Prognostic Factors in Ossiculoplasty: A Statistical Staging System","volume":"22","author":"Dornhoffer","year":"2001","journal-title":"Otol. Neurotol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1097\/00129492-200211000-00004","article-title":"Preliminary ossiculoplasty results using the Kurz titanium prostheses","volume":"23","author":"Krueger","year":"2002","journal-title":"Otol. Neurotol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1097\/00005537-200401000-00011","article-title":"Results with titanium ossicular reconstruction prostheses","volume":"114","author":"Gardner","year":"2004","journal-title":"Laryngoscope"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1097\/00005537-200004000-00005","article-title":"Hearing results in pediatric patients with chronic otitis Media after ossicular reconstruction with partial ossicular replacement prostheses and total ossicular replacement prostheses","volume":"110","author":"Murphy","year":"2000","journal-title":"Laryngoscope"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1055\/s-2000-298","article-title":"Rekonstruktion der gehorknochelchenkette mit titan-Implantaten. ergebnisse einer multicenter-Studie","volume":"79","author":"Begall","year":"2000","journal-title":"Laryngorhinootologie"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/j.medengphy.2009.06.009","article-title":"Finite element analysis of the middle ear transfer functions and related pathologies","volume":"31","author":"Zhao","year":"2009","journal-title":"Med. Eng. Phys."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Zahnert, T., Bornitz, M., and Huttenbrink, K.B. (2003, January 9\u201312). Calculation and experiments on the influence of prosthesis couplin to the middle ear transfer function. Proceedings of the 3rd Symposium on Middle Ear Mechanics in Research and Otology: Matsuyama, Ehime, Japan.","DOI":"10.1142\/9789812703019_0012"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1007\/s001060050033","article-title":"Standardisierte Messungen der Schall\u00fcbertragung verschiedener Mittelohrprothesen","volume":"48","author":"Meister","year":"2000","journal-title":"HNO"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s001060050232","article-title":"Messung der Schwingungseigenschaften von Mittelohrimplantaten mit einem mechanischen Mittelohrmodell","volume":"46","author":"Meister","year":"1998","journal-title":"HNO"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1007\/s004050050123","article-title":"Standardized measurements of the sound transmission of middle ear implants using a mechanical middle ear model","volume":"256","author":"Meister","year":"1999","journal-title":"Eur. Arch. Otorhinolaryngol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Suaste-G\u00f3mez, E., Rodr\u00edguez-Rold\u00e1n, G., Reyes-Cruz, H., and Ter\u00e1n-Jim\u00e9nez, O. (2016). Developing an ear prosthesis fabricated in polyvinylidene fluoride by a 3D printer with sensory intrinsic properties of pressure and temperature. Sensors, 16.","DOI":"10.3390\/s16030332"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4548","DOI":"10.1021\/cm100023p","article-title":"Toxicity of silver nanoparticles increases during storage because of slow dissolution under release of silver ions","volume":"22","author":"Kittler","year":"2010","journal-title":"Chem. Mater."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1007\/s12262-012-0726-y","article-title":"Nanotechnology for Surgeons","volume":"75","author":"Shrikant","year":"2013","journal-title":"Indian J. Surg."},{"key":"ref_15","first-page":"4777","article-title":"Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures","volume":"7","author":"Mendieta","year":"2012","journal-title":"Int. J. Nanomed."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1093\/jac\/dkn034","article-title":"Antimicrobial surface functionalization of plastic catheters by silver nanoparticles","volume":"61","author":"Roe","year":"2008","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_17","first-page":"65","article-title":"A review of materials used in middle ear prosthetics","volume":"70","year":"2018","journal-title":"Ceram. Mater."},{"key":"ref_18","first-page":"164","article-title":"Titanium middle ear prostheses in staged ossiculoplasty: Does mass really matter?","volume":"28","author":"Hales","year":"2007","journal-title":"Am. J. Otolaryngol. Head Neck Med. Surg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1053\/ajot.2002.124191","article-title":"Ossicular reconstruction using hydroxyapatite prostheses with interposed cartilage","volume":"23","author":"Kobayashi","year":"2002","journal-title":"Am. J. Otolaryngol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"15","DOI":"10.5152\/tao.2015.775","article-title":"Titanium versus Hydroxyapatite prostheses: Comparison of hearing and anatomical outcomes after ossicular chain reconstruction","volume":"53","author":"Ocak","year":"2015","journal-title":"Turk. Arch. Otorhinolaryngol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1097\/mao.0b013e31815c2575","article-title":"Titanium CliP piston versus platinum-ribbon Teflon piston: Piston and fenestra size affect air\u2013bone gap","volume":"29","author":"Mangham","year":"2008","journal-title":"Otol. Neurotol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1159\/000058021","article-title":"Histopathology of biocompatible hydroxylapatite-polyethylene composite in ossiculoplasty","volume":"64","author":"Meijer","year":"2002","journal-title":"ORL"},{"key":"ref_23","first-page":"2455","article-title":"Tragal cartilage versus polytetrafluoroethylene (TEFLON) partial ossicular replacement prosthesis (PORP): A comparative study of outcomes of ossiculoplasty","volume":"1","author":"Pathan","year":"2016","journal-title":"Int. J. Innov. Res. Med. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6603","DOI":"10.1016\/j.apsusc.2007.01.062","article-title":"Rapid prototyping of ossicular replacement prostheses","volume":"253","author":"Ovsianikov","year":"2007","journal-title":"Appl. Surf. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Maassen, M.M., Lowenheim, H., Pfister, M., Herberhold, S., Jorge, J.R., Baumann, I., Nusser, A., Zimmermann, R., Brosch, S., and Zenner, H.P. (2005). Surgical-handling properties of the titanium prosthesis in ossiculoplasty. ENT Ear Nose Throat J., 142\u2013149.","DOI":"10.1177\/014556130508400310"},{"key":"ref_26","first-page":"1","article-title":"Passive and active middle ear implants","volume":"8","author":"Beutner","year":"2009","journal-title":"GMS Curr. Top. Otorhinolaryngol. Head Neck Surg."},{"key":"ref_27","first-page":"315","article-title":"Synthesis of antimicrobial silver nanoparticles through a photomediated reaction in an aqueous environment","volume":"11","author":"Banasiuk","year":"2016","journal-title":"Int. J. Nanomed."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Zi\u0105bka, M., Dziadek, M., Menaszek, E., Banasiuk, R., and Kr\u00f3licka, A. (2017). Middle ear prosthesis with bactericidal efficacy\u2014In vitro investigation. Molecules, 22.","DOI":"10.3390\/molecules22101681"},{"key":"ref_29","unstructured":"(2016). PN-EN ISO 10993\u201310996: 2016, Biological Evaluation of Medical Devices. Tests for Local Effects after Implantation, International Organization for Standardization."},{"key":"ref_30","unstructured":"(2012). Polish Norm PN-EN ISO 527\u20131. Plastics. Determination of Mechanical Properties at Static Stretching. General Rules, International Organization for Standardization."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3237","DOI":"10.1021\/am5079732","article-title":"Chemical functionalization of graphene to augment stem cell osteogenesis and inhibit biofilm formation on polymer composites for orthopedic applications","volume":"7","author":"Kumar","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.joca.2006.10.010","article-title":"Elevated high-sensitivity C-reactive protein levels are associated with local inflammatory findings in patients with osteoarthritis","volume":"15","author":"Pearlea","year":"2007","journal-title":"Osteoarthr. Cartil."},{"key":"ref_33","first-page":"196","article-title":"Preliminary biological evaluation of polyoxymethylene\/nanosilver composites","volume":"12","author":"Mertas","year":"2009","journal-title":"Eng. Biomater."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Hardes, J., Streitburger, A., and Ahrens, H. (2007). The influence of elementary silver versus titanium on osteoblasts behaviour in vitro using human osteosarcoma cell lines. Sarcoma.","DOI":"10.1155\/2007\/26539"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1097\/00003086-198111000-00018","article-title":"Antibacterial and osteoinductive properties of demineralized bone matrix treated with silver","volume":"161","author":"Kramer","year":"1981","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1258\/la.2008.007152","article-title":"Experimental middle ear surgery in rabbits: A new approach for reconstructing the ossicular chain","volume":"43","author":"Stieve","year":"2009","journal-title":"Lab. Anim."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1080\/00016480601053032","article-title":"Histological evaluation of novel ossicular chain replacement prostheses: An animal study in rabbits","volume":"127","author":"Turck","year":"2007","journal-title":"Acta Oto-Laryngol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/S0142-9612(02)00408-8","article-title":"Soft-tissue response to injectable calcium phosphate cements","volume":"24","author":"Ooms","year":"2003","journal-title":"Biomaterials"},{"key":"ref_39","first-page":"e22","article-title":"Radiological and micro-computed tomography analysis of the bone at dental implants inserted 2, 3 and 4 mm apart in a minipig model with platform switching incorporated","volume":"25","author":"Elian","year":"2012","journal-title":"Clin. Oral Implants Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"41842","DOI":"10.1038\/srep41842","article-title":"Correlative imaging of the murine hind limb vasculature and muscle tissue by microct and light microscopy","volume":"7","author":"Schaad","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5407","DOI":"10.1007\/s10853-005-4339-8","article-title":"The effect of filler particle size on the antibacterial properties of compounded polymer\/silver fibers","volume":"40","author":"Jeong","year":"2005","journal-title":"J. Mater. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.jconrel.2008.05.020","article-title":"Antibiotic-eluting medical devices for various applications","volume":"130","author":"Zilberman","year":"2008","journal-title":"J. Control. Release"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1007\/s12034-015-0959-z","article-title":"Effect of silver nanoparticle on the properties of poly(methyl methacrylate) nanocomposite network made by in situ photoiniferter-mediated photopolymerization","volume":"38","author":"Makvandi","year":"2015","journal-title":"Bull. Mater. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1016\/j.biomaterials.2004.05.030","article-title":"Silver ion release from antimicrobial polyamide\/silver composites","volume":"26","author":"Kumar","year":"2005","journal-title":"Biomaterials"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2051","DOI":"10.1021\/la703085e","article-title":"Antibacterial properties of novel poly(methyl methacrylate) nanofiber containing silver nanoparticles","volume":"24","author":"Kong","year":"2008","journal-title":"Langmuir"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.1039\/c2ob06920b","article-title":"Resorcinarene bis-crown silver complexes and their application as antibacterial langmuir\u2212blodgett films","volume":"10","author":"Helttunen","year":"2012","journal-title":"Org. Biomol. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.jfda.2014.01.010","article-title":"Molecular toxicity mechanism of nanosilver","volume":"22","author":"McShan","year":"2014","journal-title":"J. Food Drug Anal."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"171113","DOI":"10.1098\/rsos.171113","article-title":"Silver nanoparticles as a medical device in healthcare settings: A five-step approach for candidate screening of coating agents","volume":"5","author":"Marassi","year":"2018","journal-title":"R. Soc. Open Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"461","DOI":"10.4012\/dmj.28.461","article-title":"Effect of surface roughness on initial responses of osteoblast-like cells on two types of zirconia","volume":"28","author":"Yamashita","year":"2009","journal-title":"Dent. Mater. J."},{"key":"ref_50","first-page":"e88","article-title":"In vitro preliminary study of osteoblast response to surface roughness of titanium discs and topical application of melatonin","volume":"20","year":"2015","journal-title":"Med. Oral Patol. Oral Cir. Bucal"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.nantod.2014.06.003","article-title":"Nanomedicine in the management of microbial infection\u2014Overview and perspectives","volume":"9","author":"Zhu","year":"2014","journal-title":"Nano Today"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1590\/1678-775720160185","article-title":"Silver nanoparticle incorporation effect on mechanical and thermal properties of denture base acrylic resins","volume":"24","author":"Dede","year":"2016","journal-title":"J. Appl. Oral Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"45807","DOI":"10.1002\/app.45807","article-title":"Comparison of mechanical properties of heat-polymerized acrylic resin with silver nanoparticles added at different concentrations and sizes","volume":"135","author":"Oyar","year":"2018","journal-title":"J. Appl. Polym. Sci."}],"container-title":["Nanomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4991\/8\/10\/764\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:22:46Z","timestamp":1760196166000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4991\/8\/10\/764"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,27]]},"references-count":53,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2018,10]]}},"alternative-id":["nano8100764"],"URL":"https:\/\/doi.org\/10.3390\/nano8100764","relation":{},"ISSN":["2079-4991"],"issn-type":[{"value":"2079-4991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,27]]}}}