{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T02:59:14Z","timestamp":1792897154484,"version":"4.5.0"},"reference-count":56,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T00:00:00Z","timestamp":1564099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute Of Allergy And Infectious Diseases of the National Institutes of Health","award":["R21AI111159"],"award-info":[{"award-number":["R21AI111159"]}]},{"name":"NIH-NIGMS-RISE","award":["1R25GM106995-01"],"award-info":[{"award-number":["1R25GM106995-01"]}]},{"name":"National Science Foundation (NSF)-CREST","award":["HRD-1241701"],"award-info":[{"award-number":["HRD-1241701"]}]},{"name":"NSF-HBCU-RISE","award":["HRD-1646729"],"award-info":[{"award-number":["HRD-1646729"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nanomaterials"],"abstract":"<jats:p>Inflammation, as induced by the presence of cytokines and chemokines, is an integral part of chlamydial infections. The anti-inflammatory cytokine, interleukin (IL)-10, has been reported to efficiently suppress the secretion of inflammatory cytokines triggered by Chlamydia in mouse macrophages. Though IL-10 is employed in clinical applications, its therapeutic usage is limited due to its short half-life. Here, we document the successful encapsulation of IL-10 within the biodegradable polymeric nanoparticles of PLA-PEG (Poly (lactic acid)-Poly (ethylene glycol), to prolong its half-life. Our results show the encapsulated-IL-10 size (~238 nm), zeta potential (\u221214.2 mV), polydispersity index (0.256), encapsulation efficiency (~77%), and a prolonged slow release pattern up to 60 days. Temperature stability of encapsulated-IL-10 was favorable, demonstrating a heat capacity of up to 89 \u00b0C as shown by differential scanning calorimetry analysis. Encapsulated-IL-10 modulated the release of IL-6 and IL-12p40 in stimulated macrophages in a time- and concentration-dependent fashion, and differentially induced SOCS1 and SOCS3 as induced by chlamydial stimulants in macrophages. Our finding offers the tremendous potential for encapsulated-IL-10 not only for chlamydial inflammatory diseases but also biomedical therapeutic applications.<\/jats:p>","DOI":"10.3390\/nano9081074","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T08:45:39Z","timestamp":1564130739000},"page":"1074","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Prolonged Release and Functionality of Interleukin-10 Encapsulated within PLA-PEG Nanoparticles"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5640-1872","authenticated-orcid":false,"given":"Skyla A.","family":"Duncan","sequence":"first","affiliation":[{"name":"Center for NanoBiotechnology &amp; Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurabh","family":"Dixit","sequence":"additional","affiliation":[{"name":"Center for NanoBiotechnology &amp; Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6270-2946","authenticated-orcid":false,"given":"Rajnish","family":"Sahu","sequence":"additional","affiliation":[{"name":"Center for NanoBiotechnology &amp; Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Martin","sequence":"additional","affiliation":[{"name":"Center for NanoBiotechnology &amp; Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieudonn\u00e9 R.","family":"Baganizi","sequence":"additional","affiliation":[{"name":"Center for NanoBiotechnology &amp; Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elijah","family":"Nyairo","sequence":"additional","affiliation":[{"name":"Center for NanoBiotechnology &amp; Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois","family":"Villinger","sequence":"additional","affiliation":[{"name":"New Iberia Research Center, University of Louisiana at Lafayette, 4401 W Admiral Doyle Drive, New Iberia, LA 70560, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3571-2222","authenticated-orcid":false,"given":"Shree R.","family":"Singh","sequence":"additional","affiliation":[{"name":"Center for NanoBiotechnology &amp; Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vida A.","family":"Dennis","sequence":"additional","affiliation":[{"name":"Center for NanoBiotechnology &amp; Life Sciences Research, Department of Biological Sciences, Alabama State University, 915 South Jackson Street, Montgomery, AL 36104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,26]]},"reference":[{"key":"ref_1","first-page":"303","article-title":"Genital Chlamydia trachomatis: An update","volume":"138","author":"Malhotra","year":"2013","journal-title":"Indian J. 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