{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T12:36:17Z","timestamp":1765370177475,"version":"build-2065373602"},"reference-count":59,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T00:00:00Z","timestamp":1681776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondazione Fibrosi Cistica (FFC)","award":["FFC#7\/2018","FISR2020IP_04128","CIB-Unife-2020","FAR-AF-Unife-2018","FAR-AF-Unife-2019","FAR-AF-Unife-2020"],"award-info":[{"award-number":["FFC#7\/2018","FISR2020IP_04128","CIB-Unife-2020","FAR-AF-Unife-2018","FAR-AF-Unife-2019","FAR-AF-Unife-2020"]}]},{"name":"MUR-FISR COVID-miRNAPNA","award":["FFC#7\/2018","FISR2020IP_04128","CIB-Unife-2020","FAR-AF-Unife-2018","FAR-AF-Unife-2019","FAR-AF-Unife-2020"],"award-info":[{"award-number":["FFC#7\/2018","FISR2020IP_04128","CIB-Unife-2020","FAR-AF-Unife-2018","FAR-AF-Unife-2019","FAR-AF-Unife-2020"]}]},{"name":"Interuniversity Consortium for Biotechnology (CIB)","award":["FFC#7\/2018","FISR2020IP_04128","CIB-Unife-2020","FAR-AF-Unife-2018","FAR-AF-Unife-2019","FAR-AF-Unife-2020"],"award-info":[{"award-number":["FFC#7\/2018","FISR2020IP_04128","CIB-Unife-2020","FAR-AF-Unife-2018","FAR-AF-Unife-2019","FAR-AF-Unife-2020"]}]},{"name":"University Fund for Scientific Research FAR","award":["FFC#7\/2018","FISR2020IP_04128","CIB-Unife-2020","FAR-AF-Unife-2018","FAR-AF-Unife-2019","FAR-AF-Unife-2020"],"award-info":[{"award-number":["FFC#7\/2018","FISR2020IP_04128","CIB-Unife-2020","FAR-AF-Unife-2018","FAR-AF-Unife-2019","FAR-AF-Unife-2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ncRNA"],"abstract":"<jats:p>(1) Background: MicroRNAs are involved in the expression of the gene encoding the chloride channel CFTR (Cystic Fibrosis Transmembrane Conductance Regulator); the objective of this short report is to study the effects of the treatment of bronchial epithelial Calu-3 cells with molecules mimicking the activity of pre-miR-145-5p, pre-miR-335-5p, and pre-miR-101-3p, and to discuss possible translational applications of these molecules in pre-clinical studies focusing on the development of protocols of possible interest in therapy; (2) Methods: CFTR mRNA was quantified by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR). The production of the CFTR protein was assessed by Western blotting; (3) Results: The treatment of Calu-3 cells with agomiR-145-5p caused the highest inhibition of CFTR mRNA accumulation and CFTR production; (4) Conclusions: The treatment of target cells with the agomiR pre-miR-145-5p should be considered when CFTR gene expression should be inhibited in pathological conditions, such as polycystic kidney disease (PKD), some types of cancer, cholera, and SARS-CoV-2 infection.<\/jats:p>","DOI":"10.3390\/ncrna9020029","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T01:09:22Z","timestamp":1681866562000},"page":"29","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Cystic Fibrosis Transmembrane Conductance Regulator Gene (CFTR) Is under Post-Transcriptional Control of microRNAs: Analysis of the Effects of agomiRNAs Mimicking miR-145-5p, miR-101-3p, and miR-335-5p"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9242-1766","authenticated-orcid":false,"given":"Chiara","family":"Papi","sequence":"first","affiliation":[{"name":"Department of Life Sciences and Biotechnology, Section of Biochemistry and Molecular Biology, University of Ferrara, 44121 Ferrara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Gasparello","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Biotechnology, Section of Biochemistry and Molecular Biology, University of Ferrara, 44121 Ferrara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6131-3727","authenticated-orcid":false,"given":"Matteo","family":"Zurlo","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Biotechnology, Section of Biochemistry and Molecular Biology, University of Ferrara, 44121 Ferrara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2949-7191","authenticated-orcid":false,"given":"Lucia Carmela","family":"Cosenza","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Biotechnology, Section of Biochemistry and Molecular Biology, University of Ferrara, 44121 Ferrara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Gambari","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Biotechnology, Section of Biochemistry and Molecular Biology, University of Ferrara, 44121 Ferrara, Italy"},{"name":"Research Center on Innovative Therapies for Cystic Fibrosis, University of Ferrara, 44121 Ferrara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7638-515X","authenticated-orcid":false,"given":"Alessia","family":"Finotti","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Biotechnology, Section of Biochemistry and Molecular Biology, University of Ferrara, 44121 Ferrara, Italy"},{"name":"Research Center on Innovative Therapies for Cystic Fibrosis, University of Ferrara, 44121 Ferrara, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1038\/nrg1379","article-title":"MicroRNAs: Small RNAs with a big role in gene regulation","volume":"5","author":"He","year":"2004","journal-title":"Nat. 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