{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T16:50:42Z","timestamp":1778691042612,"version":"3.51.4"},"reference-count":31,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T00:00:00Z","timestamp":1724630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"NEI","doi-asserted-by":"publisher","award":["5K08EY023982"],"award-info":[{"award-number":["5K08EY023982"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"NEI","doi-asserted-by":"publisher","award":["R01EY029675"],"award-info":[{"award-number":["R01EY029675"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"NEI","doi-asserted-by":"publisher","award":["P30 EY007003"],"award-info":[{"award-number":["P30 EY007003"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"NEI","doi-asserted-by":"publisher","award":["EY020895"],"award-info":[{"award-number":["EY020895"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"NEI","doi-asserted-by":"publisher","award":["EY033527"],"award-info":[{"award-number":["EY033527"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Eye Institute","award":["5K08EY023982"],"award-info":[{"award-number":["5K08EY023982"]}]},{"name":"National Eye Institute","award":["R01EY029675"],"award-info":[{"award-number":["R01EY029675"]}]},{"name":"National Eye Institute","award":["P30 EY007003"],"award-info":[{"award-number":["P30 EY007003"]}]},{"name":"National Eye Institute","award":["EY020895"],"award-info":[{"award-number":["EY020895"]}]},{"name":"National Eye Institute","award":["EY033527"],"award-info":[{"award-number":["EY033527"]}]},{"name":"NIH","award":["5K08EY023982"],"award-info":[{"award-number":["5K08EY023982"]}]},{"name":"NIH","award":["R01EY029675"],"award-info":[{"award-number":["R01EY029675"]}]},{"name":"NIH","award":["P30 EY007003"],"award-info":[{"award-number":["P30 EY007003"]}]},{"name":"NIH","award":["EY020895"],"award-info":[{"award-number":["EY020895"]}]},{"name":"NIH","award":["EY033527"],"award-info":[{"award-number":["EY033527"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurology International"],"abstract":"<jats:p>Our previous study discussed crystallin family induction in an experimental rat model of retinal detachment. Therefore, we attempted to evaluate the role of \u03b1-crystallin in photoreceptor survival in an experimental model of retinal detachment, as well as its association with the intrinsically neuroprotective protein Fas-apoptotic inhibitory molecule 2 (FAIM2). Separation of retina and RPE was induced in rat and mouse eyes by subretinal injection of hyaluronic acid. Retinas were subsequently analyzed for the presence \u03b1A-crystallin (HSPB4) and \u03b1B-crystallin (HSPB5) proteins using immunohistochemistry and immunoblotting. Photoreceptor death was analyzed using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) staining and cell counts. The 661W cells subjected to FasL were used as a cell model of photoreceptor degeneration to assess the mechanisms of the protective effect of \u03b1A-crystallin and its dependence on its phosphorylation on T148. We further evaluated the interaction between FAIM2 and \u03b1A-crystallin using a co-immunoprecipitation assay. Our results showed that \u03b1-crystallin protein levels were rapidly induced in response to retinal detachment, with \u03b1A-crystallin playing a particularly important role in protecting photoreceptors during retinal detachment. Our data also show that the photoreceptor intrinsically neuroprotective protein FAIM2 is induced and interacts with \u03b1-crystallins following retinal detachment. Mechanistically, our work also demonstrated that the phosphorylation of \u03b1A-crystallin is important for the interaction of \u03b1A-crystallin with FAIM2 and their neuroprotective effect. Thus, \u03b1A-crystallin is involved in the regulation of photoreceptor survival during retinal detachment, playing a key role in the stabilization of FAIM2, serving as an important modulator of photoreceptor cell survival under chronic stress conditions.<\/jats:p>","DOI":"10.3390\/neurolint16050068","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T11:58:46Z","timestamp":1724759926000},"page":"905-917","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["HSPB4\/CRYAA Protect Photoreceptors during Retinal Detachment in Part through FAIM2 Regulation"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7154-2024","authenticated-orcid":false,"given":"Cagri G.","family":"Besirli","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI 48105, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2738-3799","authenticated-orcid":false,"given":"Madhu","family":"Nath","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI 48105, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyu","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI 48105, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mercy","family":"Pawar","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI 48105, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela M.","family":"Myers","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI 48105, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Zacks","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI 48105, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3956-1908","authenticated-orcid":false,"given":"Patrice E.","family":"Fort","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI 48105, USA"},{"name":"Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48105, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"E312","DOI":"10.1503\/cmaj.191337","article-title":"Retinal detachment","volume":"192","author":"Kwok","year":"2020","journal-title":"CMAJ"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1001\/archopht.125.10.1389","article-title":"Role of the fas-signaling pathway in photoreceptor neuroprotection","volume":"125","author":"Zacks","year":"2007","journal-title":"Arch. 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Int."}],"container-title":["Neurology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2035-8377\/16\/5\/68\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:42:44Z","timestamp":1760110964000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2035-8377\/16\/5\/68"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,26]]},"references-count":31,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["neurolint16050068"],"URL":"https:\/\/doi.org\/10.3390\/neurolint16050068","relation":{},"ISSN":["2035-8377"],"issn-type":[{"value":"2035-8377","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,26]]}}}