{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T23:28:19Z","timestamp":1793834899462,"version":"4.6.1"},"reference-count":37,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006472","name":"Grigore T. Popa\u201d University of Medicine and Pharmacy","doi-asserted-by":"publisher","award":["10060\/15.05.2025"],"award-info":[{"award-number":["10060\/15.05.2025"]}],"id":[{"id":"10.13039\/501100006472","id-type":"DOI","asserted-by":"publisher"}]},{"award":["10060\/15.05.2025"],"award-info":[{"award-number":["10060\/15.05.2025"]}],"id":[{"id":"https:\/\/ror.org\/03hd30t45","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurology International"],"abstract":"<jats:p>Background: Standardized and ethically compliant animal models remain essential for improving translational research in Alzheimer\u2019s disease. Although A\u03b21\u201342-induced rodent models are widely used, methodological variability continues to limit reproducibility. Methods: We explored the feasibility of a stereotactic intrahippocampal A\u03b21\u201342 rat model established by bilaterally injecting pre-aggregated peptide into the hippocampus of adult Sprague Dawley rats. Model feasibility and targeting accuracy were assessed intraoperatively. Cognitive performance was evaluated using the Y-maze for spatial recognition memory and the novel object recognition (NOR) test. Histological examination was performed using hematoxylin\u2013eosin (H&amp;E) and Congo red staining to assess cytoarchitecture and to provide supportive evidence of amyloid-like deposits. Results: The surgical procedure was well-tolerated, and the injected animals showed reduced performance in behavioural testing, including reduced spatial recognition memory in the Y-maze and decreased discrimination indices in the NOR test. The animals also showed histological changes, including Congo red-positive birefringent structures consistent with amyloid-like congophilic material. Conclusions: This study presents a feasible experimental framework for intrahippocampal A\u03b21\u201342 administration, showing behavioural and histological changes under the present experimental conditions. However, further validation, including sham-operated controls and molecular characterization, will be required before these findings can be interpreted as specific to A\u03b2-driven pathology.<\/jats:p>","DOI":"10.3390\/neurolint18050079","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T17:26:18Z","timestamp":1777051578000},"page":"79","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cognitive and Histological Methodological Framework for an Intrahippocampal A\u03b21\u201342 Rat Model of Alzheimer\u2019s Disease"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6510-4220","authenticated-orcid":false,"given":"Loredana Mariana","family":"Agavriloaei","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5719-4083","authenticated-orcid":false,"given":"Bogdan Florin","family":"Iliescu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania"},{"name":"Department of Neurosurgery, \u201cProf. Dr. N. Oblu\u201d Emergency Clinical Hospital, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4440-9628","authenticated-orcid":false,"given":"Gabriela Dumitri\u021ba","family":"Stanciu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6396-0105","authenticated-orcid":false,"given":"Ivona","family":"Costachescu","sequence":"additional","affiliation":[{"name":"Advanced Research and Development Center for Experimental Medicine \u201cProf. Ostin C. Mungiu\u201d\u2014CEMEX, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4734-4509","authenticated-orcid":false,"given":"Andrei","family":"Szilagyi","sequence":"additional","affiliation":[{"name":"Advanced Research and Development Center for Experimental Medicine \u201cProf. Ostin C. Mungiu\u201d\u2014CEMEX, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria-Raluca","family":"Gogu","sequence":"additional","affiliation":[{"name":"Advanced Research and Development Center for Experimental Medicine \u201cProf. Ostin C. Mungiu\u201d\u2014CEMEX, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9868-1999","authenticated-orcid":false,"given":"Bogdan Ionel","family":"Tamba","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania"},{"name":"Advanced Research and Development Center for Experimental Medicine \u201cProf. Ostin C. Mungiu\u201d\u2014CEMEX, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1216-3459","authenticated-orcid":false,"given":"Mihaela Dana","family":"Turliuc","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania"},{"name":"Department of Neurosurgery, \u201cProf. Dr. N. Oblu\u201d Emergency Clinical Hospital, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1159\/000543578","article-title":"Global Burden of Alzheimer\u2019s Disease and Other Dementias during 1990\u20132021: A Global Burden of Disease and Risk Factors Study 2021-Based Study","volume":"60","author":"Wang","year":"2025","journal-title":"Neuroepidemiology"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"(2025). 2025 Alzheimer\u2019s disease facts and figures. Alzheimer\u2019s Dement., 21, e70235.","DOI":"10.1002\/alz.70235"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e6068","DOI":"10.1002\/gps.6068","article-title":"Variability in Alzheimer\u2019s disease mortality from European vital statistics, 2012\u20132020","volume":"39","author":"Zuin","year":"2024","journal-title":"Int. J. 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